General

Yuan Zhiming   Professor Wuhan Institute of Virology, CAS  Tel: 86-27-87198195

Fax: 86-27-87199480

E-mail: yzm@wh.iov.cn

Address: No.44 Xiao Hong Shan, Wuchang District, Wuhan, Hubei

Zip code: 430071

Research Areas

 The research group works on the diagnosis and drug discovery of aborviruses, genomics, insecticidal proteins and their mode of action, genetically modification of entomopathogenic bacteria (Bacillus spp), as well as the production, standardization and application of bio-pesticide and other microbial agents.

Education

1996~ 1999:      Ph.D. student on molecular pathology and genetic engineering, College of Biology Science, Sun Yat-sen University

1984~1987:      Graduate Student on microbiology physiology,    Biology Department, Yunnan University

1980~1984:       Undergraduate Student on biology, Biology Department,  Hubei University

 

Experience

Work Experience

1999.5-Present:    Professor on molecular biology of Bacillus sp. disease vector control and laboratory biosafety management, Wuhan Institute of Virology, Chinese Academy of Sciences.

2001.07~2001.10: Visiting Scholar on molecular biology of entomopathogenic bacteria in Danish Institute of Environmental Research.

1999.01-1999.12: Researcher on molecular biology of entomopathogenic bacteria, Royal Agricultural and Veterinary University, Denmark.

1996.11~1999.04: Associate Professor on molecular pathology and genetic engineering, Wuhan Institute of Virology, CAS.

1995.9~1996.10:   Visiting Scholar on molecular biology of Bacillus sphaericus, BIOTROP-IGENPAM, CIRAD, Montpellier; Unite des Bacteries Entopathogenes, Institut Pasteur, Paris, France.

1989.12~1995.09: Assistant Researcher on isolation, identification, classification, toxin biochemistry, production and application of B. sphaericus and B. thuringiensis, Wuhan Institute of Virology, CAS.

1987.09~1989.11: Assistant Professor on yeast and bacteria protoplast formation, regeneration and fusion, Biology Department, Yunnan University.

Publications

   
Papers

1)  Guo QY, Cai QX, Yan JP, Hu XM, Zheng DS, Yuan ZM*Single nucleotide deletion of cqm1 gene results in the development of resistance to Bacillus sphaericus in Culex quinquefasciatus. J Insect Physiol. 2013,59(9): 967-973

2)   Fan Jingjing, Yi Liu, Xuping Xie, Bo Zhang and Zhiming Yuan*, Inhibition of Japanese Encephalitis Virus Infection by Flavivirus Recombinant E Protein Domain IIIVirologica Sinica, 2013, 28 (3):152-160

3)   Chao Shan, Xiaodan Li, Chenglin Deng, Baodi Shang, Linlin Xu, Hanqing Ye, Zhiming Yuan and Bo Zhang, Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase, Virologica Sinica, 201328 (3):161-166

4)   Gao Q, Zheng D, Yuan Z*, Substrate preference of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase in Burkholderia thailandensis, FEMS Microbiol Lett. 2013339(2):110-6 IF: 2.49

5)   Shang B, Deng C, Ye H, Xu W, Yuan Z, Shi PY, Zhang BDevelopment and characterization of a stable eGFP enterovirus 71 for antiviral screening, Antiviral Res. 2013, 97(2):198-205 (IF: 3.925)

6)   Fan Jia, Jingjing Fan, Bo Zhang, Zhiming Yuan*Mutagenesis of D80-82 and G83 residues in West Nile virus NS2B: Effects on NS2B-NS3 activity and viral replicationVirologica Sinica2013, 28(1):16-23

7)   Li J, Yang L, Hu X, Zheng D, Yan J, Yuan Z*Nanoscale mono- and multi-layer cylinder structures formed by recombinant S-layer proteins of mosquitocidal Bacillus sphaericus C3-41Appl Microbiol Biotechnol. 2013, 97(16): 7275-83 (IF: 3.689)

8)   Xie X, Gayen S, Kang C, Yuan Z, Shi PY. Membrane topology and function of dengue virus NS2A protein, J Virol. 201387(8):4609-22(IF: 5.076)

9)  Wu Y, Hu X, Ge Y, Zheng D, Yuan Z*. Generation of mariner-based transposon insertion mutant library of Bacillus sphaericus 2297 and investigation of genes involved in sporulation and mosquito-larvicidal crystal protein synthesis. FEMS Microbiol Lett. 2012 330(2):105-12.

10)  Huang Y, Wei H, Wang Y, Shi Z, Raoul H, Yuan Z*. Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays. Virol Sin. 2012, 27(5):273-7.

11)  Li J, Huang C, Zheng D, Wang Y, Yuan Z*CcpA-mediated enhancement of sugar and amino acid metabolism in Lysinibacillus sphaericus by NMR-based metabolomics. J Proteome Res. 2012, 11(9):4654-61.

12)  Zhang W, Xu S, Li J, Shen X, Wang Y, Yuan Z*, 2011, Modulation of a thermoregulated type VI secretion system by AHL-dependent Quorum Sensing in Yersinia pseudotuberculosis. Arch Microbiol. 193(5):351-63.

13)  Ge Y, Hu X, Zheng D, Wu Y, Yuan Z*. 2011, Allelic Diversity and Population Structure of Bacillus sphaericus Revealed by Multilocus Sequence Typing. Appl Environ Microbiol. 77 (5): 5553–5556.

14)  Grant D, Tan GK, Qing M, Ng JK, Yip A, Zou G, Xie X, Yuan Z, Schreiber MJ, Schul W, Shi PY, Alonso S. 2011, A Single Amino Acid in Nonstructural Protein NS4B Confers Virulence to Dengue Virus in AG129 Mice through Enhancement of Viral RNA Synthesis. J. Virol., 85(15):7775-87.

15)  Xie X, Wang QY, Xu HY, Qing M, Kramer L, Yuan Z, Shi PY, 2011, Inhibition of Dengue Virus by Targeting Viral NS4B Protein. J. Virol. Aug 24.

16)  Yuan Yongming, Dasheng Zheng, Xiaomin Hu, Quanxin Cai, and Zhiming Yuan*. 2010, Conjugative Transfer of Insecticidal Plasmid pHT73 from Bacillus thuringiensis to B. anthracis and Compatibility of This Plasmid with pXO1 and pXO2. Appl Environ Microbiol. 76(2): 468–4733.801, 17%

17)  Ma Guangqiang, Zheng Dashen, Cai Quanxin, Yuan Zhiming*, 2010, Prevalence of Burkholderia pseudomallei in Guangxi, China, Epidemiol. Infect., 13837-39(2.360)

18)  Zhou Guoping, Dasheng Zheng, Lina Dou, Quanxin Cai, Zhiming Yuan*, 2010, Occurrence of psychrotolerant Bacillus cereus group strains in ice creams, International Journal of Food Microbiology, 137:143–146 (2.753, 7%)

19)  Qing M, Liu W, Yuan Z, Gu F, Shi PY. 2010, A high-throughput assay using dengue-1 virus-like particles for drug discovery. Antiviral Res. 2010  86(2):163-71

20)  Jia F, Zou G, Fan J, Yuan Z*2010, Identification of palmatine as an inhibitor of West Nile virus, Arch Virol. 155(8):1325-9

21)  Qing M, Zou G, Wang QY, Xu HY, Dong H, Yuan Z, Shi PY, 2010, Characterization of dengue virus resistance to brequinar in cell culture, Antimicrob Agents Chemother. 54:3686-95.

22)  Zou Gang, Francesc Puig-Basagoiti, Bo Zhang, Min Qing, Liqiang Chen, Krzysztof W. Pankiewicz, Krzysztof  Felczak, Zhiming Yuan, Pei-Yong Shi, 2009, A single-amino acid substitution in West Nile virus 2K peptide between NS4A and NS4B confers resistance to lycorine a flavivirus inhibitor, Virology 384 242–252.

23)  Zou G, Zhang B, Lim PY, Yuan Z, Bernard KA, Shi PY. 2009, Exclusion of West Nile virus superinfection through RNA replication. J Virol. 83(22):11765-76

24)  Puig-Basagoiti F, Qing M, Dong H, Zhang B, Zou G, Yuan Z, Shi PY. 2009, Identification and characterization of inhibitors of West Nile virus, Antiviral Res. 83: 71-9.

25)  Qing M, Yang F, Zhang B, Zou G, Robida JM, Yuan Z, Tang H, Shi PY. 2009, Cyclosporine inhibits flavivirus replication through blocking the interaction between host cyclophilins and viral NS5 protein. Antimicrob Agents Chemother. 53(8):3226-35.

26)  Qing Min Yuan Zhiming, Shi Peiyong, 2009, A Virus Specific Serological Diagnosis of Flavivirus Infection Using Virus-like Particles. Virologica Sinica, 24:136-145

27)  Liu Haizhou, Yan Jianping, Wang Qing, Karlson UG, Zou Gang, Yuan Zhiming*. 2009, Biodegradation of Methyl tert-Butyl Ether by Enriched Bacterial Culture.Curr Microbiol. 59:30-34.

28)  Ma Guangqiang, Zheng Dashen, Cai Quanxin, Yuan Zhiming*, 2009, Prevalence of Burkholderia pseudomallei in Guangxi, China, Epidemiol. Infect., 19:1-3.

29)  Li Jia, Hu Xiaomin, Yan Jianping, Yuan Zhiming*, 2009, Species-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41. Appl Environ Microbiol. 75(12):3891-5.

30)  Dou Jianlin, Janet Daly, Zhiming Yuan, Tao Jing and Tom Salomon, 2009, Bacterial cell surface display: a method for studying Japanese Encephalitis Virus pathogenicity Jpn. J. Infect. Dis. 62:402:408.

31)  Hu X, Fan W, Han B, Liu H, Liu D, Zheng D, Li Q, Yan J, Berry C, Yuan Z*. 2008, Complete genome sequences of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with closely related Bacillus species. J Bacteriol. 190: 2892-902.

32)  Zhou G, Liu H, He J, Yuan Y, Yuan Z*. 2008, The occurrence of Bacillus cereus, B. thuringiensis and B. mycoides in Chinese pasteurized full fat milk. Int J Food Microbiol. 121:195200.

33)  Hu X, Li J, Hansen BM, Yuan Z*. 2008Phylogenetic analysis and heterologous expression of surface layer protein SlpC of Bacillus sphaericus C3-41. Biosci Biotech Bioch. 72:1257-63.

34)  Zhou G, Yan J, Dasheng Z, Zhou X, Yuan Z*. 2008, The residual occurrences of Bacillus thuringiensis biopesticides in food and beverages. Int J Food Microbiol. 127:68-72.

35)  Zhang JT, Yan JP, Zheng DS, Sun YJ, Yuan ZM*. 2008, Expression of mel gene improves the UV resistance of Bacillus thuringiensis.  J Appl Microbiol. 105:151-7.

36)  Bingke Bai, Xinya Lua,Jin Meng, Qinxue Hu, Panyong Mao, Baojing Lu, Ze Chen, Zhiming Yuan, Hanzhong Wang. 2008, Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like coronavirus generates humoral and cellular immune responses, Molecular Immunology, 45: 868–875.

Research Interests

Molecular biology of entomopathogenic bacteria (Bacillusspp.)

Tropical Disease vector control with microbial agents

Diagnosis of aborviruses and other emerging disease pathogens

Laboratory biorisk management and applied biosafety research

Students

已指导学生

郑大胜  博士研究生  071005-微生物学  

胡晓敏  博士研究生  071005-微生物学  

韩蓓  博士研究生  071005-微生物学  

杨艳坤  博士研究生  071005-微生物学  

蔡亚君  博士研究生  071005-微生物学  

孙运军  博士研究生  071005-微生物学  

刘海舟  博士研究生  071005-微生物学  

薛建丽  硕士研究生  071005-微生物学  

高雅懿  硕士研究生  071005-微生物学  

魏素珍  硕士研究生  071005-微生物学  

吴恩应  硕士研究生  071005-微生物学  

郭青云  硕士研究生  071005-微生物学  

窦建琳  博士研究生  071005-微生物学  

袁永明  博士研究生  071005-微生物学  

周帼萍  博士研究生  071005-微生物学  

刘朋明  博士研究生  071005-微生物学  

邹罡  博士研究生  071005-微生物学  

马广强  博士研究生  071005-微生物学  

谢作蓉  硕士研究生  071005-微生物学  

陶琰  硕士研究生  071005-微生物学  

熊武辉  硕士研究生  071005-微生物学  

贾凡  博士研究生  071005-微生物学  

范晶晶  博士研究生  071005-微生物学  

黎佳  博士研究生  071005-微生物学  

高强  博士研究生  071005-微生物学  

青敏  博士研究生  071005-微生物学  

陈慧子  硕士研究生  071005-微生物学  

蒋玲芝  硕士研究生  071010-生物化学与分子生物学  

李晶  硕士研究生  071010-生物化学与分子生物学  

邹晶  硕士研究生  071005-微生物学  

谢旭平  博士研究生  071010-生物化学与分子生物学  

巫益鸣  博士研究生  071005-微生物学  

韩娜  博士研究生  071005-微生物学  

郭青云  博士研究生  071005-微生物学  

刘艺  博士研究生  071005-微生物学  

杨玲玲  硕士研究生  071005-微生物学  

葛勇  博士研究生  071005-微生物学  

张伟鹏  硕士研究生  071010-生物化学与分子生物学  

孙娇  硕士研究生  071005-微生物学  

石廷玉  博士研究生  071005-微生物学  

赵妮  博士研究生  071005-微生物学  

张振清  硕士研究生  071005-微生物学  

徐凯  硕士研究生  071005-微生物学  

邹晶  博士研究生  071005-微生物学  

宰俊杰  博士研究生  071005-微生物学  

葛赛  博士研究生  071005-微生物学  

史琛彦  硕士研究生  071005-微生物学  

梅小芬  硕士研究生  085238-生物工程  

胡轶敏  博士研究生  071005-微生物学  

伊尹华  硕士研究生  071005-微生物学  

付攀  博士研究生  071005-微生物学  

田申  博士研究生  071005-微生物学  

耿佩玲  博士研究生  071005-微生物学  

赵娜  硕士研究生  085238-生物工程  

刘孝云  博士研究生  071005-微生物学  

现指导学生

叶国国  博士研究生  071005-微生物学  

董倩男  硕士研究生  071010-生物化学与分子生物学  

孙佳慧  硕士研究生  071005-微生物学  

赵路  博士研究生  071005-微生物学  

任南洁  硕士研究生  071005-微生物学  

万小芙  博士研究生  071005-微生物学  

梁梦莹  硕士研究生  085238-生物工程  

王小钰  硕士研究生  100103-病原生物学  

Honors & Distinctions

2000             Award of Outstanding Innovative Youth in Science and Technology

2007              Medal of Youth of May 4th in Hubei Province

2008             Award of Distinguished Young Specialist in Hubei Province.

2012             State Council Expert for Special Allowance