微生物次级代谢的合成与调控

   

071007-遗传学

微生物次级代谢的合成与调控

1994-09--1997-05   中国科学院微生物研究所   硕士研究生
1987-09--1991-07   北京大学分校   学士

   

2007-05~现在, 中国科学院微生物研究所，微生物资源前期开发国家重点实验室, 副研究员
2001-07~2003-12,Department of Molecular Microbiology, John Innes Centre, UK, 访问学者
1999-05~2001-02,北京华大基因研究中心, 合作研究
1996-11~2007-04,中国科学院微生物研究所，微生物代谢工程研究中心, 助理研究员
1991-08~1996-10,中国科学院微生物研究所，遗传与育种研究室, 实习研究员

微生物遗传与分子生物学

   

（1） Reconstruction of a hybrid nucleoside antibiotic gene cluster based on scarless modification of large DNA fragment, Sci China Life Sciences, 2017, 通讯作者
（2） Activation and molecular mechanism of a cryptic oviedomycin biosynthetic gene cluster via the disruption of a global regulatory gene--adpA in Streptomyces ansochromogenes, Journal of Biological Chemistry, 2017, 通讯作者
（3） Specialised metabolites regulating antibiotic biosynthesis in Streptomyces spp, FEMS Microbiol Rev, 2016, 第 3 作者
（4） Genome engineering and direct cloning gene cluster via phage phiBT1 integrase-mediated site-specific recombination in Streptomyces, Sci Rep, 2015, 第 3 作者
（5） GouR, a TetR-family t ranscriptional regulator, coordinates the biosynthesis and export of gougerotin in Streptomyces graminearus, Appl Environ Microb, 2014, 第 2 作者
（6） Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes, Sci China Life Sciences, 2013, 通讯作者
（7） Improvement of gougerotin and nikkomycin production by engineering their biosynthetic gene clusters, Appl Microbiol Biotechnol, 2013, 第 4 作者
（8） Importance and regulation of inositol biosynthesis during growth and differentiation of Streptomyces coelicolor, Mol Microbiol, 2012, 第 1 作者
（9） Hybrid antibiotics with the nikkomycin nucleoside and polyoxin peptidyl moieties, Metab Eng, 2011, 第 3 作者
（10） SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes, BMC Microbiol, 2011, 第 4 作者
（11） Hybrid antibiotics with the nikkomycin nucleoside and polyoxin peptidyl moieties, Metab Eng, 2011, 第 3 作者
（12） Characterization of EndoTT, a novel single-stranded DNA specific endonuclease from Thermoanaerobacter tengcongensis, Nucleic Acids Res, 2010, 第 4 作者
（13） Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production, Microb Cell Fact, 2010, 第 5 作者
（14） SCO3900, co-transcripted with three downstream genes, is involved in the differentiation of Streptomyces coelicolor, Current Microbiology, 2010, 第 2 作者
（15） The pleiotropic regulator AdpA-L directly controls the pathway-specific activator of nikkomycin biosynthesis in Streptomyces ansochromogenes, Mol Microbiol, 2009, 第 4 作者
（16） The pleiotropic regulator AdpA-L directly controls the pathway-specific activator of nikkomycin biosynthesis in Streptomyces ansochromogenes, Mol Microbiol, 2009, 第 4 作者
（17） Selectively improving nikkomycin Z production by blocking the imidazolone biosynthetic pathway of nikkomycin X and uracil feeding in Streptomyces ansochromogenes, Microb Cell Fact, 2009, 第 5 作者
（18） polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. asoensis, Microbiology-SGM, 2009, 第 3 作者
（19） The tyrosine degradation gene hppD is transcriptionally activated by HpdA and repressed by HpdR in Streptomyces coelicolor, while hpdA is negatively autoregulated and repressed by HpdR, Molecular Microbiology, 2007, 第 4 作者
（20） An unusual response regulator influences sporulation at early and late stages in Streptomyces coelicolor, JOURNAL OF BACTERIOLOGY, 2007, 第 1 作者
（21） SanM catalyzes the formation of 4-pyridyl-2-oxo-4-hydroxyisovalerate in nikkomycin biosynthesis by interacting with SanN, Biochemical and Biophysical Research Communications, 2007, 第 3 作者
（22） SanT, a bidomain protein, is essential for nikkomycin biosynthesis of Streptomyces ansochromogenes, Biochemical and Biophysical Research Communications, 2007, 第 2 作者
（23） Identification and characterization of sawC, a whiA-like gene, essential for sporulation in Streptomyces ansochromogenes, Arch Microbiol, 2007, 第 3 作者
（24） SanJ, an ATP-dependent picolinate-CoA ligase, catalyzes the conversion of picolinate to picolinate-CoA during nikkomycin biosynthesis in S. ansochromogenes, Metabolic Engineering, 2006, 第 3 作者
（25） A pathway-specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development, Molecular Microbiology, 2005, 第 2 作者
（26） Lambda red-mediated genetic manipulation of antibiotic producing Streptomyces, Adv Appl Microbiol, 2004, 第 4 作者
（27） A novel Streptomyces gene, samR, with different effects on differentiation of Streptomyces ansochromogenes and Streptomyces coelicolor, Arch Microbiol, 2002, 第 2 作者
（28） A Complete Sequence of the T. tengcongensis Genome, Genome Research, 2002, 第 1 作者
（29） The Streptomyces coelicolor sporulation-specific Sigma-WhiG form of RNA polymerase transcribes a gene encoding a ProX-like protein that is dispensable for sporulation, Gene, 1998, 第 3 作者

   

（ 1 ） 圈卷产色链霉菌隐性次级代谢基因簇的调控机制, 主持, 国家级, 2014-01--2017-12
（ 2 ） 合成微生物体系的适配性研究, 参与, 国家级, 2013-01--2017-08
（ 3 ） 尼可霉素生物合成中信号分子介导的SabR调控的分子机制, 参与, 国家级, 2016-01--2019-12

已指导学生

朱宇  硕士研究生  071007-遗传学  

卓继明  硕士研究生  071007-遗传学  

徐晶晶  博士研究生  071007-遗传学