1）干细胞分化、去分化的表观遗传机制

2）人类疾病的干细胞模型、动物模型的建立和致病机制研究

3）肿瘤发生发展的表观遗传机理

博士生，硕博连读生

071009-细胞生物学

干细胞分化、去分化的表观遗传机制
人类疾病的干细胞模型、动物模型的建立和致病机制研究
肿瘤发生发展的表观调控机制

2002-09--2005-07   中科院植物研究所   博士

博士

博士

肿瘤细胞生物学

2005-2006  美国国立卫生研究院                                               博士后

2006-2008  美国宾夕法尼亚州立大学                                        博士后

2008-2011  美国国立卫生研究院                                            Research Fellow

2011年10月- 至今，中国科学院广州生物医药与健康研究院      研究员

[1] Dong, Xiaotao, Guo, Rong, Ji, Tianrong, Zhang, Jie, Xu, Jun, Li, Yaoyi, Sheng, Yingliang, Wang, Yuxiang, Fang, Ke, Wen, Yulin, Liu, Bei, Hu, Gongcheng, Deng, Hongkui, Yao, Hongjie. YY1 safeguard multidimensional epigenetic landscape associated with extended pluripotency. NUCLEIC ACIDS RESEARCH. 2022, [2] Song, Yawei, Liang, Zhengyu, Zhang, Jie, Hu, Gongcheng, Wang, Juehan, Li, Yaoyi, Guo, Rong, Dong, Xiaotao, Babarinde, Isaac A, Ping, Wangfang, Sheng, YingLiang, Li, Huanhuan, Chen, Zhaoming, Gao, Minghui, Chen, Yang, Shan, Ge, Zhang, Michael Q, Hutchins, Andrew P, Fu, XiangDong, Yao, Hongjie. CTCF functions as an insulator for somatic genes and a chromatin remodeler for pluripotency genes during reprogramming. CELL REPORTS[J]. 2022, 39(1): http://dx.doi.org/10.1016/j.celrep.2022.110626.
[3] Xia, Qing, Cui, Guizhong, Fan, Ye, Wang, Xiuqin, Hu, Gongcheng, Wang, Lisha, Luo, Xi, Yang, Lele, Cai, Qingqing, Xu, Kaibiao, Guo, Wenjing, Gao, Minghui, Li, Yingying, Wu, Ji, Li, Wei, Chen, Jiayu, Qi, Huayu, Peng, Guangdun, Yao, Hongjie. RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes. CELL PROLIFERATION[J]. 2021, 54(5): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088469/.
[4] Li, Yaoyi, Song, Yawei, Xu, Wei, Li, Qin, Wang, Xinxiu, Li, Kuan, Wang, Juehan, Liu, Zicong, Velychko, Sergiy, Ye, Rong, Xia, Qing, Wang, Lei, Guo, Rong, Dong, Xiaotao, Zheng, Zhikai, Dai, Yushuang, Li, Haojie, Yao, Mingze, Xue, Yuanchao, Schoeler, Hans R, Sun, Qianwen, Yao, Hongjie. R-loops coordinate with SOX2 in regulating reprogramming to pluripotency. SCIENCE ADVANCES[J]. 2020, 6(24): http://dx.doi.org/10.1126/sciadv.aba0777.
[5] Hu, Gongcheng, Dong, Xiaotao, Gong, Shixin, Song, Yawei, Hutchins, Andrew P, Yao, Hongjie. Systematic screening of CTCF binding partners identifies that BHLHE40 regulates CTCF genome-wide distribution and long-range chromatin interactions. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(17): 9606-9620, http://dx.doi.org/10.1093/nar/gkaa705.
[6] Song, Yawei, Hu, Gongcheng, Jia, Jinping, Yao, Mingze, Wang, Xiaoshan, Lu, Wenliang, Hutchins, Andrew P, Chen, Jiekai, Ozato, Keiko, Yao, Hongjie. DNA Damage Induces Dynamic Associations of BRD4/P-TEFb With Chromatin and Modulates Gene Transcription in a BRD4-Dependent and -Independent Manner. FRONTIERS IN MOLECULAR BIOSCIENCES[J]. 2020, 7: https://doaj.org/article/e6b32cd4213e4416bc3471ab63f7695e.
[7] 姚红杰. RNA结合蛋白复合物调控体细胞重编程的机制研究. 2020, 1-, https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CPFD&dbname=CPFDTEMP&filename=IGSS202010001058&v=Mjg3OTVKSVYwVkxDcllmYkc0SE5ITnI0OUZaZXNLQkJOS3VoZGhuajk4VG5qcXF4ZEVlTU9VS3JpZlplWnVGQ2pzVTdm.
[8] 王秀芹, 尹梦然, 夏晴, 王丽莎, 秦宝明, 姚红杰. RNA结合蛋白在多能干细胞命运转变中的研究进展. 中国细胞生物学学报[J]. 2020, 42(1): 145-151, http://lib.cqvip.com/Qikan/Article/Detail?id=7101519083.
[9] Li, Jiao, Huang, Kaimeng, Hu, Gongcheng, Babarinde, Isaac A, Li, Yaoyi, Dong, Xiaotao, Chen, YuSheng, Shang, Liping, Guo, Wenjing, Wang, Junwei, Chen, Zhaoming, Hutchins, Andrew P, Yang, YunGui, Yao, Hongjie. An alternative CTCF isoform antagonizes canonical CTCF occupancy and changes chromatin architecture to promote apoptosis. NATURE COMMUNICATIONS[J]. 2019, 10(1): http://dx.doi.org/10.1038/s41467-019-08949-w.
[10] Liu, Zicong, Yao, Mingze, Yao, Hongjie, Hu, Gongcheng, Qin, Baoming. Generation of Rybp homozygous knockout murine ES cell line GIBHe001-A-1 by using CRISPR/Cas9 technology. STEM CELL RESEARCH[J]. 2019, 41: http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000506008200060.
[11] Huanhuan, Li, Ping, Lai, Jinping, Jia, Yawci, Song, Qing, Xia, Kaimeng, Huang, Na, He, Wangfang, Ping, Jiayu, Chen, Zhongzhou, Yang, Jiao, Li, Mingze, Yao, Xiaotao, Dong, Jicheng, Zhao, Chunhui, Hou, Miguel, A.Esteban, Shaorong, Gao, Duanqing, Pei, Andreew, P.Hutchins, 姚红杰. RNA解旋酶DDX5通过miRNA调控RYBP依赖和非依赖于PRCl的双重功能而抑制体细胞重编程. 科学新闻. 2018, 59-59, http://lib.cqvip.com/Qikan/Article/Detail?id=675303226.
[12] Mingze Yao, Xueke Zhou, Jiajian Zhou, Shixin Gong, Gongcheng Hu, Jiao Li, Kaimeng Huang, Ping Lai, Guang Shi, Andrew P Hutchins, Hao Sun, Huating Wang, Hongjie Yao. PCGF5 is required for neural differentiation of embryonic stem cells. NATURE COMMUNICATIONS[J]. 2018, 9(1): http://dx.doi.org/10.1038/s41467-018-03781-0.
[13] Ping, Wangfang, Hu, Jian, Hu, Gongcheng, Song, Yawei, Xia, Qing, Yao, Mingze, Gong, Shixin, Jiang, Cizhong, Yao, Hongjie. Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs. CELL DEATH & DISEASE[J]. 2018, 9(2): http://www.corc.org.cn/handle/1471x/2159729.
[14] Ding, Jian, Yu, Chao, Sui, Yi, Wang, Lingyao, Yang, Yang, Wang, Fei, Yao, Hongjie, Xing, Feiyang, Liu, Hongshen, Li, Yana, Shah, Junaid Ali, Cai, Yong, Jin, Jingji. The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin. FEBS JOURNAL[J]. 2018, 285(17): 3270-3285, https://www.webofscience.com/wos/woscc/full-record/WOS:000443814200010.
[15] Wangfang Ping, Jian Hu, Gongcheng Hu, Yawei Song, Qing Xia, Mingze Yao, Shixin Gong, Cizhong Jiang, 姚红杰. Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs. Cell Death & Disease. 2018, 9(2): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5833453/.
[16] Li, Wufeng, Shang, Liping, Huang, Kaimeng, Li, Jiao, Wang, Zhibin, Yao, Hongjie. Identification of critical base pairs required for CTCF binding in motif M1 and M2. PROTEIN & CELLnull. 2017, 8(7): 544-549, http://lib.cqvip.com/Qikan/Article/Detail?id=672744440.
[17] Peng, Xianlu L, So, Karl K, He, Liangqiang, Zhao, Yu, Zhou, Jiajian, Li, Yuying, Yao, Mingze, Xu, Bo, Zhang, Suyang, Yao, Hongjie, Hu, Ping, Sun, Hao, Wang, Huating. MyoD- and FoxO3-mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation. NUCLEIC ACIDS RESEARCH[J]. 2017, 45(15): 8785-8805, http://dx.doi.org/10.1093/nar/gkx488.
[18] Shan, Yongli, Liang, Zechuan, Xing, Qi, Zhang, Tian, Wang, Bo, Tian, Shulan, Huang, Wenhao, Zhang, Yanqi, Yao, Jiao, Zhu, Yanling, Huang, Ke, Liu, Yujian, Wang, Xiaoshan, Chen, Qianyu, Zhang, Jian, Shang, Bizhi, Li, Shengbiao, Shi, Xi, Liao, Baojian, Zhang, Cong, Lai, Keyu, Zhong, Xiaofen, Shu, Xiaodong, Wang, Jinyong, Yao, Hongjie, Chen, Jiekai, Pei, Duanqing, Pan, Guangjin. PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naive ESCs. NATURE COMMUNICATIONS[J]. 2017, 8: https://www.webofscience.com/wos/woscc/full-record/WOS:000411526700017.
[19] Li, Huanhuan, Lai, Ping, Jia, Jinping, Song, Yawei, Xia, Qing, Huang, Kaimeng, He, Na, Ping, Wangfang, Chen, Jiayu, Yang, Zhongzhou, Li, Jiao, Yao, Mingze, Dong, Xiaotao, Zhao, Jicheng, Hou, Chunhui, Esteban, Miguel A, Gao, Shaorong, Pei, Duanqing, Hutchins, Andrew P, Yao, Hongjie. RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and - Independent Functions. CELL STEM CELL[J]. 2017, 20(4): 462-+, http://dx.doi.org/10.1016/j.stem.2016.12.002.
[20] Liu, Jing, Han, Qingkai, Peng, Tianran, Peng, Meixiu, Wei, Bei, Li, Dongwei, Wang, Xiaoshan, Yu, Shengyong, Yang, Jiaqi, Cao, Shangtao, Huang, Kaimeng, Hutchins, Andrew Paul, Liu, He, Kuang, Junqi, Zhou, Zhiwei, Chen, Jing, Wu, Haoyu, Guo, Lin, Chen, Yongqiang, Chen, You, Li, Xuejia, Wu, Hongling, Liao, Baojian, He, Wei, Song, Hong, Yao, Hongjie, Pan, Guangjin, Chen, Jiekai, Pei, Duanqing. The oncogene c-Jun impedes somatic cell reprogramming. NATURE CELL BIOLOGY[J]. 2015, 17(7): 856-867, http://ir.foo.ac.cn/handle/2SETSVCV/407.
[21] Huang, Kaimeng, Zhang, Xiaobai, Shi, Jiejun, Yao, Mingze, Lin, Jiannan, Li, Jiao, Liu, He, Li, Huanhuan, Shi, Guang, Wang, Zhibin, Zhang, Biliang, Chen, Jiekai, Pan, Guangjin, Jiang, Cizhong, Pei, Duanqing, Yao, Hongjie. Dynamically reorganized chromatin is the key for the reprogramming of somatic cells to pluripotent cells. SCIENTIFIC REPORTS[J]. 2015, 5: http://dx.doi.org/10.1038/srep17691.
[22] Liu, Longqi, Xu, Yan, He, Minghui, Zhang, Meng, Cui, Fenggong, Lu, Leina, Yao, Mingze, Tian, Weihua, Benda, Christina, Zhuang, Qiang, Huang, Zhijian, Li, Wenjuan, Li, Xiangchun, Zhao, Ping, Fan, Wenxia, Luo, Zhiwei, Li, Yuan, Wu, Yasong, Hutchins, Andrew P, Wang, Dongye, Tse, HungFat, Schambach, Axel, Frampton, Jon, Qin, Baoming, Bao, Xichen, Yao, Hongjie, Zhang, Biliang, Sun, Hao, Pei, Duanqing, Wang, Huating, Wang, Jun, Esteban, Miguel A. Transcriptional Pause Release Is a Rate-Limiting Step for Somatic Cell Reprogramming. CELL STEM CELL[J]. 2014, 15(5): 574-588, http://dx.doi.org/10.1016/j.stem.2014.09.018.
[23] Huang, Kaimeng, Jia, Jinping, Wu, Changwei, Yao, Mingze, Li, Min, Jin, Jingji, Jiang, Cizhong, Cai, Yong, Pei, Duanqing, Pan, Guangjin, Yao, Hongjie. Ribosomal RNA Gene Transcription Mediated by the Master Genome Regulator Protein CCCTC-binding Factor (CTCF) Is Negatively Regulated by the Condensin Complex. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2013, 288(36): 26067-26077, http://dx.doi.org/10.1074/jbc.M113.486175.
[24] 徐国良. Vitamin C modulates Tet1 function during somatic cell reprogramming. Nature Genetics[J]. 2013, 45(12): 1504-U140, http://ir.foo.ac.cn/handle/2SETSVCV/44.
[25] 徐国良. Vitamin C modulates TET1 function during somatic cell reprogramming. NATURE GENETICS[J]. 2013, 45(12): 1504-U140, http://ir.foo.ac.cn/handle/2SETSVCV/44.
[26] Huang Kaimeng, Jia Jinping, Wu Changwei, Yao Mingze, Li Min, Jin Jingji, Jiang Cizhong, Cai Yong, Pei Duanqing, Pan Guangjin, Yao Hongjie. Ribosomal RNA Gene Transcription Mediated by the Master Genome Regulator Protein CCCTC-binding Factor (CTCF) Is Negatively Regulated by the Condensin Complex *. The Journal of Biological Chemistry. 288(36): 26067-26077, http://dx.doi.org/10.1074/jbc.M113.486175.

   

（ 1 ） 院启动, 主持, 市地级, 2011-10--2016-10
（ 2 ） 浓缩素II型复合物对染色质绝缘功能的调控及其机理研究, 主持, 国家级, 2013-01--2016-12
（ 3 ） PCGF5介导的组蛋白H2A泛素化修饰对小鼠ES细胞多能性维持和神经分化的调控及其机理研究, 主持, 国家级, 2015-01--2018-12
（ 4 ） 干细胞衰老的机理研究, 参与, 国家级, 2015-05--2016-12
（ 5 ） 组蛋白与DNA 修饰的动态调控机制研究, 主持, 国家级, 2016-07--2020-12
（ 6 ） PCGF5对组蛋白泛素化修饰及对胚胎干细胞命运调控的机理研究, 主持, 省级, 2016-01--2019-12
（ 7 ） 基于表观基因编辑技术和小分子化合物优化提高神经干细胞获得效率及其转化应用研究, 主持, 省级, 2016-09--2018-12
（ 8 ） 以排斥性导向分子B为靶点抑制肺癌转移的ceRNA模型机制研究, 参与, 省级, 2017-05--2020-04
（ 9 ） 神经元再生的表观机理及应用, 主持, 部委级, 2017-12--2019-12
（ 10 ） 神经干细胞的功能维持和调控研究, 主持, 省级, 2019-01--2021-12
（ 11 ） 细胞命运决定的表观遗传机理, 主持, 国家级, 2020-01--2024-12
（ 12 ） 染色质三维基因组技术, 参与, 部委级, 2020-01--2020-12

（1）染色质结构变化调控细胞命运   中国生理学会干细胞生理专业委员会第二届学术年会   2020-11-27
（2）YY1 facilitates maintenance of EPSC identity by regulating epigenetic crosstalk and CTCF-mediated chromatin looping   中国细胞生物学学会染色质生物学分会第五次学术研讨会   2020-11-04
（3）Epigenetic reprogramming mediated by RNA-associated Proteins   第5届全国发育生物学大会   2020-10-27
（4）RNA结合蛋白调控体细胞重编程不同机制的研究   中国生物化学与分子生物学会2020年全国学术会议   2020-10-22
（5）An alternative CTCF isoform antagonizes canonical CTCF occupancy and changes chromatin architecture to modulate cell fate   2019-10-10
（6）Non-canonical PRC1 and Chromatin Organization in Stem Cell Fate Determination    四川省细胞生物学学会2018年度学术大会   2018-11-22
（7）Roles of Non-canonical PRC1 in Somatic Cell Reprogramming and Neural Differentiation of mESCs   2018-11-19
（8）An alternative CTCF isoform antagonizes canonical CTCF occupancy and changes chromatin architecture to promote cell apoptosis   2018-10-23
（9）Roles of non-canonical PRC1 in somatic cell reprogramming and neural differentiation of mESCs   中国细胞生物学学会2017全国学术大会   2017-09-26
（10）RNA Helicase DDX5 Controls Mouse Somatic Cell Reprogramming by Modulating the Context- Specific Functions of RYBP   中国干细胞第7届年会   2017-07-06
（11）RNA Binding Protein DDX5 Inhibits Reprogramming to Pluripotency by miRNA-based Repression of RYBP and its PRC1-dependent and -independent Functions   2017-06-14
（12）RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-based Repression of RYBP and its PRC1-dependent and -independent Functions   ➢ 中国细胞生物学学会首届染色质生物学大会   2017-04-14

已指导学生

黄开猛  博士研究生  071009-细胞生物学  

胡功成  博士研究生  071009-细胞生物学  

李欢欢  博士研究生  071009-细胞生物学  

李娇  博士研究生  071009-细胞生物学  

龚士欣  博士研究生  071009-细胞生物学  

夏晴  博士研究生  071009-细胞生物学  

董晓涛  博士研究生  071009-细胞生物学  

王丽莎  硕士研究生  071009-细胞生物学  

郭蓉  博士研究生  071009-细胞生物学  

李尧益  博士研究生  071009-细胞生物学  

颜成鸿  硕士研究生  085238-生物工程  

现指导学生

平王芳  博士研究生  071009-细胞生物学  

温毓麟  博士研究生  071009-细胞生物学  

王珏涵  博士研究生  071009-细胞生物学  

章杰  博士研究生  071009-细胞生物学  

李清  博士研究生  071009-细胞生物学  

王新秀  博士研究生  071009-细胞生物学  

王钰翔  硕士研究生  071009-细胞生物学  

卢玉丽  博士研究生  071009-细胞生物学  

刘妍江  博士研究生  071009-细胞生物学  

黄赛南  博士研究生  071009-细胞生物学  

钟宏新  硕士研究生  071010-生物化学与分子生物学