鱼类原始生殖细胞起源、迁移、分化、成熟调控机制研究；

海水鱼类生殖干细胞移植技术研发与应用研究；

重要海洋动物精子保存及其应用研究；

特色海水鱼类不同生殖策略及适应性进化研究。

水产养殖      硕士1名/博士1名

070703-海洋生物学
090801-水产养殖

繁殖、发育与调控技术
生理生化过程与调控
养殖遗传学与育种生物技术

2018-10--2019-10   密歇根大学分子细胞发育生物学系   访问学者
2005-09--2008-07   中国科学院海洋研究所   博士
2002-09--2005-07   中国海洋大学   硕士
1998-09--2002-07   青岛农业大学    本科

   

2017-01~现在, 中国科学院海洋研究所, 研究员四级
2012-06~现在, 中国科学院海洋研究所, 硕导
2011-01~2016-12,中国科学院海洋研究所, 副研究员
2008-07~2010-12,中国科学院海洋研究所, 助理研究员

   

（1） 条石鲷生殖调控及规模化生产技术的建立与应用, 二等奖, 省级, 2014
（2） 杂交鲆（牙鲆♀×夏鲆♂）规模化生产技术的建立与应用, 一等奖, 市地级, 2014
（3） 国家海洋局科学技术二等奖, 二等奖, 省级, 2013

   

[1] Wang, Xueying, Liu, Qinghua, Li, Jun, Zhou, Li, Wang, Tao, Zhao, Ning. Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii. BIOLOGY OF REPRODUCTION[J]. 2023, 108(2): 338-352, http://dx.doi.org/10.1093/biolre/ioac203.
[2] Zhao, Ning, Wang, Xueying, Wang, Tao, Xu, Xiaojie, Liu, Qinghua, Li, Jun. Genome-Wide Identification of Laminin Family Related to Follicular Pseudoplacenta Development in Black Rockfish (Sebastes schlegelii). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2022, 23(18): http://dx.doi.org/10.3390/ijms231810523.
[3] Liu, Yifan, Liu, Qinghua, Xu, Shihong, Wang, Yanfeng, Feng, Chengcheng, Zhao, Chunyan, Song, Zongcheng, Li, Jun. A Deep Insight of Spermatogenesis and Hormone Levels of Aqua-Cultured Turbot (Scophthalmus maximus). FRONTIERS IN MARINE SCIENCE[J]. 2021, 7: https://doaj.org/article/821dfdb69a91444ca01dc1e85a20a85c.
[4] Zhao, Haixia, Wang, Xueying, Du, Tengfei, Gao, Guang, Wu, Lele, Xu, Shihong, Xiao, Yongshuang, Wang, Yanfeng, Liu, Qinghua, Li, Jun. Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii). THERIOGENOLOGY[J]. 2021, 166: 83-89, http://dx.doi.org/10.1016/j.theriogenology.2021.01.001.
[5] Zhou, Li, Xu, Shihong, Lin, Fan, Wang, Xueying, Wang, Yunong, Wang, Yanfeng, Yu, Daode, Liu, Qinghua, Li, Jun. Both of marine fish species Oryzias melastigma and Pagrus major all failing in early localization at embryo stage by vasa RNA. GENE[J]. 2021, 769: http://dx.doi.org/10.1016/j.gene.2020.145204.
[6] Du Tengfei, Xiao Yongshuang, Zhao Haixia, Zhou Li, Liu Qinghua, Wang Xueying, Li Jun, Xu Shihong, Wang Yanfeng, Yu Jiachen, Wu Lele, Wang Yunong, Gao Guang. Multiple Fetal Nutritional Patterns Before Parturition in Viviparous Fish Sebastes schlegelii (Hilgendorf, 1880). FRONTIERS IN MARINE SCIENCE[J]. 2021, 7: https://doaj.org/article/4505d21dcfb44b0a8960ca69258028f6.
[7] 赵海霞, 王学颖, 杜腾飞, 周莉, 徐世宏, 王彦丰, 肖永双, 刘清华, 李军. 体内受精许氏平鲉（Sebastes schlegelii）和体外受精大菱鲆（Scophthalmus maximus）精子结构及生理特性比较. 海洋与湖沼[J]. 2020, 51(6): 1513-1519, http://lib.cqvip.com/Qikan/Article/Detail?id=7103324272.
[8] 周莉, 刘清华, 李军. 鱼类生殖细胞移植技术研究及进展. 海洋科学[J]. 2020, 44(8): 48-55, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2020&filename=HYKX202008006&v=MDM3OTdvUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSN3VmWnVab0Z5L2tWcjdBTFRUQWRyRzRITkhNcDQ5Rlk=.
[9] Yang, Yang, Liu, Qinghua, Xiao, Yongshuang, Xu, Shihong, Wang, Xueying, Yang, Jingkun, Song, Zongcheng, You, Feng, Li, Jun. Effects of environmental stress (sex steroids and heat) during sex differentiation in Japanese flounder (Paralichthys olivaceus): insight from germ cell proliferation and gsdf-amh-cyp19a1a expression. AQUACULTURE[J]. 2020, 515: http://dx.doi.org/10.1016/j.aquaculture.2019.734536.
[10] Zhou, Li, Wang, Xueying, Du, Shuran, Wang, Yanfeng, Zhao, Haixia, Du, Tengfei, Yu, Jiachen, Wu, Lele, Song, Zongcheng, Liu, Qinghua, Li, Jun. Germline Specific Expression of a vasa Homologue Gene in the Viviparous Fish Black Rockfish (Sebastes schlegelii) and Functional Analysis of the vasa 3 ' Untranslated Region. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY[J]. 2020, 8: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732447/.
[11] Lv, Xuejiao, Xu, Shihong, Liu, Qinghua, Wang, Xueying, Yang, Jingkun, Song, Zongcheng, Li, Jun. Osteological ontogeny and allometric growth in larval and juvenile turbot (Scophthalmus maximus). AQUACULTURE[J]. 2019, 498: 351-363, http://dx.doi.org/10.1016/j.aquaculture.2018.08.063.
[12] 徐世宏, 王彦丰, 刘清华, 刘鹰, 王顺奎, 徐滢轩, 李军. 大西洋鲑在封闭循环水养殖模式下的生长和性腺发育研究. 海洋科学[J]. 2019, 43(11): 97-102, http://lib.cqvip.com/Qikan/Article/Detail?id=7100504351.
[13] Yang, Yang, Liu, Qinghua, Xiao, Yongshuang, Xu, Shihong, Wang, Xueying, Yang, Jingkun, Song, Zongcheng, You, Feng, Li, Jun. High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus). GENERAL AND COMPARATIVE ENDOCRINOLOGY[J]. 2019, 274: 17-25, http://dx.doi.org/10.1016/j.ygcen.2018.12.012.
[14] Lv, Xuejiao, Wang, Yunong, Xiao, Zhizhong, Liu, Qinghua, Xu, Shihong, Zhai, Jieming, Li, Jun. Skeletal ontogeny and deformity during the early fry culture process for Epinephelus lanceolatus. AQUACULTURE[J]. 2019, 508: 113-126, http://dx.doi.org/10.1016/j.aquaculture.2019.04.059.
[15] Liang Chi, Xian Li, Qinghua Liu, Ying Liu. Photoperiod may regulate growth via leptin receptor A1 in the hypothalamus andsaccus vasculosus of Atlantic salmon (Salmo salar). ANIMAL CELLS AND SYSTEMS[J]. 2019, 23(3): 200-208, https://doaj.org/article/e750f3f276f94684816d3d211278db38.
[16] Wang Yanfeng, Chi Liang, Liu Qinghua, Xiao Yongshuang, Ma Daoyuan, Xiao Zhizhong, Xu Shihong, Li Jun. Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system (RAS). JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2019, 37(1): 350-360, http://lib.cqvip.com/Qikan/Article/Detail?id=7001285153.
[17] Zhou, Li, Wang, Xueying, Liu, Qinghua, Xu, Shihong, Zhao, Haixia, Han, Mingming, Wang, Yunong, Song, Zongcheng, Li, Jun. Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3 ' Untranslated Region of nanos3 or vasa Gene. MARINE BIOTECHNOLOGY[J]. 2019, 21(5): 671-682, http://ir.qdio.ac.cn/handle/337002/162953, http://www.irgrid.ac.cn/handle/1471x/6861245, http://ir.qdio.ac.cn/handle/337002/162954, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000487127600009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=3a85505900f77cc629623c3f2907beab.
[18] Liu, Qinghua, Wang, Xueying, Xiao, Yongshuang, Zhao, Haixia, Xu, Shihong, Wang, Yanfeng, Wu, Lele, Zhou, Li, Du, Tengfei, Lv, Xuejiao, Li, Jun. Sequencing of the black rockfish chromosomal genome provides insight into spermstorage in the female ovary. DNA RESEARCH[J]. 2019, 26(6): 453-464, https://www.webofscience.com/wos/woscc/full-record/WOS:000514627800002.
[19] Yang Yang, Liu Qinghua, Ma Daoyuan, Song Zongcheng, Li Jun. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205). JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2018, 36(4): 1342-1348, http://lib.cqvip.com/Qikan/Article/Detail?id=676045557.
[20] Xue, Rui, Wang, Xueying, Xu, Shihong, Liu, Yifan, Feng, Chengcheng, Zhao, Chunyan, Liu, Qinghua, Li, Jun. Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY[J]. 2018, 226: 53-63, http://dx.doi.org/10.1016/j.cbpb.2018.08.002.
[21] Wang, Xueying, Liu, Qinghua, Xu, Shihong, Xiao, Yongshuang, Wang, Yanfeng, Feng, Chengcheng, Xue, Rui, Zhao, Haixia, Song, Zongcheng, Li, Jun. Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation. SCIENTIFIC REPORTS[J]. 2018, 8(1): http://www.irgrid.ac.cn/handle/1471x/2486215.
[22] Xiao, Yongshuang, Guan, Shuguang, Liu, Qinghua, Liu, Hongjun, Yu, Daode, Ma, Daoyuan, Xu, Shihong, Liu, Jing, Dai, Ming, Xiao, Zhizhong, Li, Jun. Genetic characteristics of broodstock and offspring of the seven-band grouper (Hyporthodus septemfasciatus) using fluorescent-AFLP markers. JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM[J]. 2018, 98(2): 261-267, https://www.webofscience.com/wos/woscc/full-record/WOS:000426876800005.
[23] Zhao Chunyan, Xu Shihong, Feng Chengcheng, Liu Yifan, Yang Yang, Wang Yanfeng, Xiao Yongshuang, Song Zongcheng, Liu Qinghua, Li Jun. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus. JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2018, 36(4): 1360-1373, http://lib.cqvip.com/Qikan/Article/Detail?id=676045559.
[24] Li Jun. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus. Chinese Journal of Oceanology and Limnology, 2017, Chinese Journal of Oceanology and Limnology. Chinese Journal of Oceanology and Limnology. 2018, [25] Rui Xue, Xueying Wang, Shihong Xu, Yifan Liu, Chengcheng Feng, Chunyan Zhao, Qinghua Liu, Jun Li. Expression profile and localization of vitellogenin mRNA and protein during ovarian development in turbot (Scophthalmus maximus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, PART B. 2018, 226: 53-63, http://dx.doi.org/10.1016/j.cbpb.2018.08.002.
[26] Yang, Yang, Liu, Qinghua, Ma, Daoyuan, Xiao, Yongshuang, Xu, Shihong, Wang, Xueying, Song, Zongcheng, You, Feng, Li, Jun. Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus). THERIOGENOLOGY[J]. 2018, 120: 68-78, http://dx.doi.org/10.1016/j.theriogenology.2018.07.041.
[27] 杨阳, 刘清华, 马道远, 宋宗诚, 李军. A potential germ cell-specific marker in Japanese flounder,Paralichthys olivaceus:identification and characterization of lymphocyte antigen 75(Ly75/CD205). 海洋湖沼学报（英文）[J]. 2018, 36(4): 1342-1348, http://lib.cqvip.com/Qikan/Article/Detail?id=676045557.
[28] Feng, Chengcheng, Xu, Shihong, Liu, Yifan, Wang, Yanfeng, Wang, Wenqi, Yang, Jingkun, Zhao, Chunyan, Liu, Qinghua, Li, Jun. Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors. FISH PHYSIOLOGY AND BIOCHEMISTRY[J]. 2018, 44(1): 35-48, http://ir.qdio.ac.cn/handle/337002/154338.
[29] Zhao, Chunyan, Liu, Qinghua, Xu, Shihong, Xiao, Yongshuang, Wang, Wenqi, Yang, Jingkun, Yang, Yang, Wang, Yanfeng, Song, Zongcheng, Li, Jun. Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205). THERIOGENOLOGY[J]. 2018, 113: 137-145, http://dx.doi.org/10.1016/j.theriogenology.2017.12.016.
[30] Li Jun. Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new stem cell specific antigen 75 (ly75/CD205). theriogenology. 2018, [31] Yang, Yang, Liu, Qinghua, Xiao, Yongshuang, Wang, Xueying, An, Hao, Song, Zongcheng, You, Feng, Wang, Yanfeng, Ma, Daoyuan, Li, Jun. Germ Cell Migration, Proliferation and Differentiation during Gonadal Morphogenesis in All-Female Japanese Flounder (Paralichthys Olivaceus). ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY[J]. 2018, 301(4): 727-741, http://dx.doi.org/10.1002/ar.23698.
[32] 吕雪娇, 王雨浓, 刘清华, 翟介明, 李军. 鞍带石斑鱼仔稚幼鱼骨骼发育与生长特性研究. 海洋科学[J]. 2018, 42(5): 116-121, http://lib.cqvip.com/Qikan/Article/Detail?id=7000889106.
[33] 王文琪, 杨敬昆, 徐世宏, 刘清华, 李军. 3种对虾精子超低温冷冻保存技术研究. 海洋科学[J]. 2017, 41(9): 81-86, http://lib.cqvip.com/Qikan/Article/Detail?id=7000480778.
[34] Yifan Liu, Daoyuan Ma, Chunyan Zhao, Zhizhong Xiao, Shihong Xu, Yongshuang Xiao, Yanfeng Wang, Qinghua Liu, Jun Li. The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P.olivaceus ♀× P.dentatus ♂) to chronic and acute heat stress. MARINE ENVIRONMENTAL RESEARCH. 2017, 129: 386-395, http://dx.doi.org/10.1016/j.marenvres.2017.06.015.
[35] Chi, Liang, Li, Xian, Liu, Qinghua, Liu, Ying. Photoperiod regulate gonad development via kisspeptin/kissr in hypothalamus and saccus vasculosus of Atlantic salmon (Salmo solar). PLOS ONE[J]. 2017, 12(2): https://www.webofscience.com/wos/woscc/full-record/WOS:000394424400008.
[36] Zhao, Chunyan, Xu, Shihong, Liu, Yifan, Wang, Yanfeng, Liu, Qinghua, Li, Jun. Gonadogenesis analysis and sex differentiation in cultured turbot (Scophthalmus maximus). FISH PHYSIOLOGY AND BIOCHEMISTRY[J]. 2017, 43(1): 265-278, http://ir.qdio.ac.cn/handle/337002/136635.
[37] Wang, Xueying, Liu, Qinghua, Xiao, Yongshuang, Yang, Yang, Wang, Yanfeng, Song, Zongcheng, You, Feng, An, Hao, Li, Jun. High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention. AQUACULTURE[J]. 2017, 479: 808-816, http://ir.qdio.ac.cn/handle/337002/143135.
[38] 韩龙江, 刘清华, 许飞, 温海深, 李军. 长牡蛎精子超低温冷冻后超微结构损伤研究. 水生生物学报[J]. 2017, 41(1): 220-227, http://lib.cqvip.com/Qikan/Article/Detail?id=671115042.
[39] Wang Xueying, Liu Qinghua, Xiao Yongshuang, Yang Yang, Wang Yanfeng, Song Zongcheng, You Feng, An Hao, Li Jun. High temperature causes masculinization of genetically female olive flounder ( Paralichthys olivaceus ) accompanied by primordial germ cell proliferation detention. AQUACULTURE[J]. 2017, [40] Liu, Yifan, Ma, Daoyuan, Zhao, Chunyan, Xiao, Zhizhong, Xu, Shihong, Xiao, Yongshuang, Wang, Yanfeng, Liu, Qinghua, Li, Jun. The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P-olivaceus female x P-dentatus male) to chronic and acute heat stress. MARINE ENVIRONMENTAL RESEARCH[J]. 2017, 129: 386-395, http://ir.qdio.ac.cn/handle/337002/143128.
[41] 丰程程, 柳意樊, 安皓, 徐世宏, 王彦丰, 宋宗诚, 刘清华, 李军. 减数分裂期雄性大菱鲆孕激素及受体基因的表达分析. 海洋科学[J]. 2017, 41(8): 91-98, http://lib.cqvip.com/Qikan/Article/Detail?id=674194257.
[42] Zhao Chunyan, Xu Shihong, Liu Yifan, Wang Yanfeng, Liu Qinghua, Li Jun. Gonadogenesis analysis and sex differentiation in cultured turbot ( Scophthalmus maximus ). FISH PHYSIOLOGY AND BIOCHEMISTRY[J]. 2017, [43] 安皓, 徐世宏, 丰程程, 王彦丰, 刘清华, 李军. 大菱鲆精子运动特征与精液pH的相关性研究. 海洋科学[J]. 2017, 41(7): 37-43, http://lib.cqvip.com/Qikan/Article/Detail?id=673547890.
[44] 薛蕊, 安皓, 刘清华, 肖志忠, 王彦丰, 李军. 斑石鲷（Oplegnathuspunctatus）雌、 雄鱼核型及Ag-NORs带型分析. 海洋与湖沼[J]. 2016, 47(3): 626-632, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5756020&detailType=1.
[45] 宋莉妮, 田永胜, 李祥孔, 李洪, 孙宗哲, 陈张帆, 李军, 刘清华, 陈松林. 钝吻黄盖鲽精子冷冻保存及生理特性分析. 农业生物技术学报[J]. 2016, 24(4): 584-592, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5653042&detailType=1.
[46] Xiao, Yongshuang, Ma, Daoyuan, Dai, Ming, Liu, Qinghua, Xiao, Zhizhong, Li, Jun, Liu, Jing, Xu, Shihong, Cheng, Qiqun. The impact of Yangtze River discharge on the genetic structure of a population of the rock bream, Oplegnathus fasciatus. MARINE BIOLOGY RESEARCH[J]. 2016, 12(4): 426-434, http://dx.doi.org/10.1080/17451000.2016.1154576.
[47] 王雨福, 肖志忠, 刘清华, 翟介明, 庞尊方, 马文辉, 马道远, 徐世宏, 肖永双, 李军. 斑石鲷早期发育的异速生长模式. 海洋科学[J]. 2016, 40(5): 43-48,  http://dx.doi.org/10.11759//hykx20141216005.
[48] Li Jun. Sperm cryopreservation in different grouper shuspecies and application in interspecifid hybridization.. Theriogenology. 2016, [49] 王学颖, 徐世宏, 刘清华, 李军. 渗透压、pH、葡萄糖及离子溶液对夏牙鲆精子激活及运动特征的影响. 海洋科学[J]. 2016, 40(3): 40-46,  http://dx.doi.org/10.11759//hykx20150305003.
[50] Liu Qinghua. Effects of chilling storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod (Gadus macrocephalus). Chinese Journal of Oceanology and Limnology. Chinese Journal of Oceanology and Limnology. 2016, [51] Xiao Yongshuang, Ma Daoyuan, Xu Shihong, Liu Qinghua, Wang Yanfeng, Xiao Zhizhong, Li Jun. Significant population genetic structure detected in the rock bream Oplegnathus fasciatus (Temminck & Schlegel, 1844) inferred from fluorescent-AFLP analysis. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2016, 34(3): 441-450, http://dx.doi.org/10.1007/s00343-016-4404-y.
[52] Wang Xueying, Shi Xuehui, Liu Yifan, Yu Daode, Guan Shuguang, Liu Qinghua, Li Jun. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus macrocephalus. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2016, 34(4): 763-771, https://www.webofscience.com/wos/woscc/full-record/WOS:000377207800013.
[53] Liu Qinghua. The dnd RNA identifies germ cell origin and migration in Olive flounder (Paralichthysolivaceus).. BioMed Research. International. 2016, [54] Wang, Xueying, Liu, Qinghua, Xiao, Yongshuang, Yang, Yang, Wang, Yanfeng, Song, Zongcheng, You, Feng, An, Hao, Xiao, Zhizhong, Xu, Shihong, Ma, Daoyuan, Li, Jun. The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus). BIOMED RESEARCH INTERNATIONAL[J]. 2015, 2015: http://www.corc.org.cn/handle/1471x/2376666.
[55] Liu Qinghua. Effects of chilling storage and cryopreservation on sperm characteristics, antioxidant enzyme activity, and lipid peroxidation in Pacific cod (Gadus macrocephalus).. 2015, [56] Liu Yifan, Ma Daoyuan, Xiao Zhizhong, Xu Shihong, Wang Yanfeng, Wang Yufu, Xiao Yongshuang, Song Zongcheng, Teng Zhaojun, Liu Qinghua, Li Jun. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2015, 33(1): 11-19, https://www.webofscience.com/wos/woscc/full-record/WOS:000351156200002.
[57] Liu, Qinghua, Wang, Xueying, Wang, Wenqi, Zhang, Xuelei, Xu, Shihong, Ma, Daoyuan, Xiao, Zhizhong, Xiao, Yongshuang, Li, Jun. Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream (Pagrus major) sperm cryopreservation. FISH PHYSIOLOGY AND BIOCHEMISTRY[J]. 2015, 41(2): 413-422, https://www.webofscience.com/wos/woscc/full-record/WOS:000351506900008.
[58] Chi, Liang, Liu, Qinghua, Xu, Shihong, Xiao, Zhizhong, Ma, Daoyuan, Li, Jun. Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY[J]. 2015, 188: 148-155, http://dx.doi.org/10.1016/j.cbpa.2015.06.034.
[59] Li Jun. Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with oliver flounder (P. olivaceus). Theriogenology. 2015, [60] Yifan Liu, Daoyuan Ma, Chunyan Zhao, Wenqi Wang, Xuelei Zhang, Xiao Liu, Ying Liu, Zhizhong Xiao, Shihong Xu, Yongshuang Xiao, Qinghua Liu, Jun Li. Histological and enzymatic responses of Japanese flounder ( Paralichthys olivaceus ) and its hybrids ( P. olivaceus ♀ × P. dentatus ♂) to chronic heat stress. FISH PHYSIOLOGY AND BIOCHEMISTRY,. 2014, 40(4): [61] 韩龙江, 刘清华, 于道德, 纪利芹, 王文琪, 刘名, 温海深, 李军. 太平洋鳕（Gadus macrocephalus）精液超低温冷冻方法的建立及精子超微结构分析. 海洋与湖沼[J]. 2014, 789-797, http://lib.cqvip.com/Qikan/Article/Detail?id=72897090504849524852484953.
[62] 迟良, 徐世宏, 肖志忠, 马道远, 林帆, 赵春彦, 肖永双, 武宁宁, 刘清华, 李军. 大菱鲆胃质子泵（H~+ /K~+ ATPase）与消化机能发生的关系. 海洋与湖沼[J]. 2014, 45(3): 644-650, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5196726&detailType=1.
[63] 肖永双, 肖志忠, 刘清华, 滕照军, 徐世宏, 马道远, 李军. 星斑川鲽、大菱鲆及其杂交后代的线粒体DNA序列比较分析. 海洋科学[J]. 2014, 38(6): 5-9,  http://dx.doi.org/10.11759/hykx20130903001.
[64] 郑君飘, 刘清华, 徐世宏, 马道远, 肖志忠, 林帆, 肖永双, 李军. 真鲷精子在外源DNA下的生理特性和转染效果. 海洋科学[J]. 2014, 38(7): 1-6,  http://dx.doi.org/10.11759/hykx20131223002.
[65] Liu, Yifan, Ma, Daoyuan, Zhao, Chunyan, Wang, Wenqi, Zhang, Xuelei, Liu, Xiao, Liu, Ying, Xiao, Zhizhong, Xu, Shihong, Xiao, Yongshuang, Liu, Qinghua, Li, Jun. Histological and enzymatic responses of Japanese flounder (Paralichthys olivaceus) and its hybrids (P-olivaceus a (TM) Euro x P-dentatus a (TM),) to chronic heat stress. FISH PHYSIOLOGY AND BIOCHEMISTRY[J]. 2014, 40(4): 1031-1041, https://www.webofscience.com/wos/woscc/full-record/WOS:000338452200004.
[66] 韩龙江, 黄雯, 刘清华, 李军, 张国范, 李军, 温海深. 太平洋牡蛎（Crassostrea gigas）精液的超低温保存研究. 海洋与湖沼[J]. 2014, 1085-1091, http://lib.cqvip.com/Qikan/Article/Detail?id=72897090504849524853485053.
[67] 隋娟, 马道远, 肖志忠, 徐世宏, 肖永双, 武宁宁, 刘清华, 李军. 褐牙鲆、夏鲆及其杂交子代核型与DAPI带型分析. 海洋科学[J]. 2014, 38(3): 69-74,  http://dx.doi.org/10.11759/hykx20130814004.
[68] 韩龙江, 刘清华, 纪利芹, 温海深, 黄雯, 张国范, 李军. 太平洋牡蛎（Crassostrea gigas）担轮幼虫的超低温保存研究. 海洋与湖沼[J]. 2014, 1258-1263, http://lib.cqvip.com/Qikan/Article/Detail?id=72897090504849524854484952.
[69] 张雪雷, 王文琪, 肖志忠, 徐长安, 蒋日进, 徐世宏, 马道远, 肖永双, 武宁宁, 刘清华, 李军. 超低温保存后大菱鲆（Scophthalmus maximus）精子的生理活性及其超微结构研究. 海洋与湖沼[J]. 2013, 44(4): 1103-1107, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=4958332&detailType=1.
[70] Liang Chi, Shihong Xu, Zhizhong Xiao, Fan Lin, Daoyuan Ma, Chunyan Zhao, Yongshuang Xiao, Qinghua Liu, Jun Li. Pepsinogen A and C genes in turbot ( Scophthalmus maximus ): Characterization and expression in early development. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, PART B. 2013, 58-65, http://dx.doi.org/10.1016/j.cbpb.2013.03.002.
[71] 肖永双, 程起群, 任桂静, 李军, 马道远, 徐世宏, 刘清华, 肖志忠, 韩志强. 中国近海条石鲷（Oplegnathus fasciatus）群体遗传多样性线粒体DNA 序列分析. 海洋与湖沼[J]. 2013, 44(2): 427-432, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=4841671&detailType=1.
[72] Sui, Juan, Liu, Qinghua, Xiao, Zhizhong, Ma, Daoyuan, Xu, Shihong, He, Tao, Liu, Yifan, Xiao, Yongshuang, Li, Jun. The viability, melanophore and embryo genesis of first- and second-generation hybrids between Paralichthys olivaceus and P. dentatus. MARINE BIOLOGY RESEARCH[J]. 2013, 9(2): 220-226, https://www.webofscience.com/wos/woscc/full-record/WOS:000311443500010.
[73] 何滔, 肖志忠, 刘清华, 李军. 条石鲷鳃的组织发育及鳃上钠钾三磷酸腺苷酶活性的早期变化. 水产学报[J]. 2013, 37(4): 520-525, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=4805046&detailType=1.
[74] Chi, Liang, Xu, Shihong, Xiao, Zhizhong, Lin, Fan, Ma, Daoyuan, Zhao, Chunyan, Xiao, Yongshuang, Liu, Qinghua, Li, Jun. Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY[J]. 2013, 165(1): 58-65, http://dx.doi.org/10.1016/j.cbpb.2013.03.002.
[75] 肖志忠, 肖永双, 任桂静, 高天翔, 涂登志, 韩志强, 马道远, 徐世宏, 刘清华, 李军. 条石鲷（Oplegnathus fasciatus）养殖群体与野生群体线粒体控制区序列遗传变异研究. 海洋与湖沼[J]. 2013, 44(1): 249-254, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=4807326&detailType=1.
[76] 刘洪军, 官曙光, 刘清华, 李军, 于道德. 美洲黑石斑精子超低温保存研究. 海洋科学[J]. 2013, 37(1): 76-80, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=4801854&detailType=1.
[77] 隋娟, 徐世宏, 王文琪, 马道远, 肖志忠, 肖永双, 刘清华, 李军. 牙鲆与夏鲆杂交及回交子代胚胎发育及早期生长比较. 海洋科学[J]. 2012, 36(6): 54-58, http://lib.cqvip.com/Qikan/Article/Detail?id=42637354.
[78] 李军, 何滔, 肖志忠, 刘清华. 条石鲷早期发育阶段的生长模式. 水产学报[J]. 2012, 36(8): 1242-1248, http://lib.cqvip.com/Qikan/Article/Detail?id=43076444.
[79] 肖永双, 马道远, 肖志忠, 刘清华, 徐世宏, 李军. 基于能量代谢酶相对活力评价大菱鲆卵质. 南方水产科学[J]. 2012, 8(2): 15-21, http://lib.cqvip.com/Qikan/Article/Detail?id=41538380.
[80] 何滔, 肖志忠, 刘清华, 马道远, 徐世宏, 肖永双, 李军. 条石鲷视觉器官早期发育的组织学观察. 海洋科学[J]. 2012, 36(3): 49-53, http://lib.cqvip.com/Qikan/Article/Detail?id=42065461.
[81] He, Tao, Xiao, Zhizhong, Liu, Qinghua, Ma, Daoyuan, Xu, Shihong, Xiao, Yongshuang, Li, Jun. Ontogeny of the digestive tract and enzymes in rock bream Oplegnathus fasciatus (Temminck et Schlegel 1844) larvae. FISH PHYSIOLOGY AND BIOCHEMISTRY[J]. 2012, 38(2): 297-308, https://www.webofscience.com/wos/woscc/full-record/WOS:000304117000002.
[82] 何滔, 肖志忠, 刘清华, 李军. 条石鲷（Oplegnathus fasciatus）心脏早期发育的组织学观察. 海洋与湖沼[J]. 2012, 43(2): 268-272, http://lib.cqvip.com/Qikan/Article/Detail?id=42006912.
[83] Lin, Fan, Xu, Shihong, Ma, Daoyuan, Xiao, Zhizhong, Zhao, Chunyan, Xiao, Yongshuang, Chi, Liang, Liu, Qinghua, Li, Jun. Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei. MOLECULAR REPRODUCTION AND DEVELOPMENT[J]. 2012, 79(11): 803-813, https://www.webofscience.com/wos/woscc/full-record/WOS:000311297000007.
[84] Lin, Fan, Liu, Qinghua, Li, Mingyou, Li, Zhendong, Hong, Ni, Li, Jun, Hong, Yunhan. Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major). INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES[J]. 2012, 8(6): 882-890, https://doaj.org/article/4843f8c1e2ad42f89827585d92acdd96.
[85] 佟雪红, 徐世宏, 刘清华, 李军, 肖志忠, 马道远. 大菱鲆早期发育过程中免疫器官的发生. 海洋科学[J]. 2011, 35(6): 62-67, http://lib.cqvip.com/Qikan/Article/Detail?id=38431960.
[86] 林帆, 刘清华, 李军, 隋娟, 肖志忠, 徐世宏, 马道远, 肖永双. 真鲷（Pagrus major）vasa基因5′端启动子克隆及表达载体构建. 海洋与湖沼[J]. 2011, 42(2): 186-192, http://lib.cqvip.com/Qikan/Article/Detail?id=38631381.
[87] 马道远, 于道德, 肖永双, 刘清华, 徐世宏, 李军, 肖志忠. 条石鲷消化道发育的组织学观察. 海洋科学[J]. 2011, 35(12): 28-35, http://lib.cqvip.com/Qikan/Article/Detail?id=40731750.
[88] 佟雪红, 马道远, 徐世宏, 刘清华, 赵春彦, 肖志忠, 肖永双, 李军. 大菱鲆（Scophthalmus maximus）胚胎发育的形态学和组织学研究. 海洋与湖沼[J]. 2011, 42(6): 844-849, http://lib.cqvip.com/Qikan/Article/Detail?id=41014029.
[89] 肖志忠, 肖永双, 马道远, 徐世宏, 刘清华, 李军. 基于线粒体DNA部分片段探讨条石鲷与斑石鲷的亲缘关系. 海洋学报[J]. 2011, 33(5): 115-123, http://lib.cqvip.com/Qikan/Article/Detail?id=39633371.
[90] Sui, Juan, Liu, Qinghua, He, Tao, Xiao, Zhizhong, Xu, Shihong, Ma, Daoyuan, Xiao, Yongshuang, Lin, Fan, Li, Jun. Analysis of the early development in first and backcross generations between Paralichthys olivaceus and Paralichthys dentatus. AFRICAN JOURNAL OF BIOTECHNOLOGY[J]. 2011, 10(68): 15438-15443, http://ir.qdio.ac.cn/handle/337002/11996.
[91] 佟雪红, 徐世宏, 刘清华, 李军, 肖志忠, 马道远. 条斑星鲽幼鱼变态期间核酸及蛋白的变化. 海洋科学[J]. 2010, 41-47, http://lib.cqvip.com/Qikan/Article/Detail?id=34250051.
[92] Liu, Qing Hua, Chen, Ya Kun, Xiao, Zhi Zhong, Li, Jun, Xu, Shi Hong, Shi, Xue Hui. Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843). AQUACULTURE RESEARCH[J]. 2010, 41(9): e89-e95, http://ir.qdio.ac.cn/handle/337002/1648.
[93] 何滔, 肖志忠, 刘清华, 李军, 赵春彦, 徐世宏, 马道远. 条石鲷鳔器官早期发育的组织学观察. 海洋科学[J]. 2010, 8-13, http://lib.cqvip.com/Qikan/Article/Detail?id=37129502.
[94] 李军, 肖志忠, 徐世宏, 马道远, 陈亚坤, 刘清华, 赵春彦. 2010长期超低温保存后真鲷精子的质量变化. 海洋科学[J]. 2010, 34(6): 50-54, http://ir.qdio.ac.cn/handle/337002/10549.
[95] 陈亚坤, 刘清华, 赵春彦, 肖志忠, 徐世宏, 马道远, 李军. 长期超低温保存后真鲷精子的质量变化. 海洋科学[J]. 2010, 50-54, http://lib.cqvip.com/Qikan/Article/Detail?id=34606472.
[96] Yu, Daode, Xiao, Zhizhong, Liu, Qinghua, Xu, Shihong, Ma, Daoyuan, Li, Jun, Webb, Kenneth A. Ontogeny of Gastrointestinal Tract in Hybrid Flounder Jasum, Paralichthys olivaceus x P-dentatus. JOURNAL OF THE WORLD AQUACULTURE SOCIETY[J]. 2010, 41(3): 344-357, https://www.webofscience.com/wos/woscc/full-record/WOS:000278567500006.
[97] Chen, Ya Kun, Liu, Qing Hua, Li, Jun, Xiao, Zhi Zhong, Xu, Shi Hong, Shi, Xue Hui, Ma, Dao Yuan. Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm. CRYOBIOLOGY[J]. 2010, 61(2): 189-193, http://ir.qdio.ac.cn/handle/337002/6139.
[98] 肖志忠, 陈雄芳, 丁福红, 刘清华, 徐世宏, 李军. 大黄鱼精液的高效超低温保存. 海洋科学[J]. 2007, 31(4): 1-4,72, http://lib.cqvip.com/Qikan/Article/Detail?id=24277433.
[99] Liu, Qing Hua, Li, Jun, Xiao, Zhi Zhong, Ding, Fu Hong, Yu, Dao De, Xu, Xi Zhang. Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major). AQUACULTURE[J]. 2007, 263(1-4): 20-25, http://ir.qdio.ac.cn/handle/337002/6071.
[100] 刘清华, 李军, 丁福红, 肖志忠, 于道德, 徐西长. 运用计算机辅助分析检测超低温保存的真鲷（Pagrosomus major）精子的质量. 自然科学进展[J]. 2006, 16(9): 1181-1185,  http://dx.doi.org/10.3321/j.issn:1002-008X.2006.09.018.
[101] 刘清华, 肖志忠, 李军, 丁福红, 于道德, 徐西长. 运用计算机辅助分析检测超低温保存的真鲷（Pagrus major）精子的质量. 自然科学进展[J]. 2006, 16(8): 282-286, http://ir.qdio.ac.cn/handle/337002/10859.
[102] Li Jun, Liu Qinghua, Zhang Shicui. Evaluation of the damage in fish spermatozoa cryopreservation. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2006, 24(4): 370-377, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=2659056&detailType=1.
[103] 刘清华, 李军, 张士璀. Evaluztion of the damage in fish spermatozoa cryopreservatio. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2006, 24(4): 370-377, http://ir.qdio.ac.cn/handle/337002/10839.
[104] Liu, Qinghua, Li, Jun, Zhang, Shicui, Ding, Fuhong, Xu, Xizhang, Xiao, Zhizhong, Xu, Shihong. An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials. JOURNAL OF THE WORLD AQUACULTURE SOCIETY[J]. 2006, 37(3): 289-297, http://ir.qdio.ac.cn/handle/337002/6149.
[105] Haixiao Zhao, Xueying Wang, Tengfei Du, Guang Gao, Lele Wu, Shihong Xu, Yongshuang Xiao, Yanfeng Wang, Qinghua Liu, Jun Li. The dynamic sperm storage process in typical viviparous teleost black rockfish, Sebastes schlegelii: evidences of sperm maturation, migration, localization before and after copulation. THERIOGENOLOGY. http://dx.doi.org/10.1016/j.theriogenology.2021.01.001.
[106] Li Zhou, Xueying Wang, Shuran Du, Shihong Xu, Yanfeng Wang, Zongcheng Song, Qinghua Liu, Jun Li. Successful transplantation of cryopreserved spermatogonia in Sebastes schlegelii: A simple and suitable alternative approach for conservation of viviparous fish. WATER BIOLOGY AND SECURITY. http://dx.doi.org/10.1016/j.watbs.2023.100142.

（1） 鱼类精子和胚胎冷冻保存理论与技术, Theory and techonology of cryopreservation of fish sperm and embryos, 中国农业出版社, 2007-07, 第 2 作者
（2） 海水鱼类精子冷冻保存和精子结构功能质量评价, Marine fish sperm cryopreservation and quality evaluation in sperm structure and function. Cryopreservation, InTech, 2012-03, 第 1 作者

   

（ 1 ） 超低温状态下活性氧对鱼类精子的损伤机制及其调控研究, 主持, 国家级, 2011-01--2013-12
（ 2 ） 牙鲆与夏鲆杂交及回交分子细胞遗传学机理研究, 主持, 省级, 2010-01--2012-12
（ 3 ） 超低温保存对海水鱼类精子质量的影响, 主持, 部委级, 2010-01--2012-12
（ 4 ） 海水鱼类原始生殖细胞生成模式及其分离和冷冻保存研究, 主持, 省级, 2012-01--2014-09
（ 5 ） 重要海水鱼类精子冷冻保存技术及种质库的建立, 主持, 市地级, 2012-01--2013-12
（ 6 ） 鲆鲽类现代产业技术体系建设, 参与, 国家级, 2011-01--2015-12
（ 7 ） 高温诱导牙鲆原始生殖细胞迁移增殖及和雄性化机制研究, 主持, 国家级, 2014-01--2017-12
（ 8 ） 中国科学院青年促进会项目, 主持, 部委级, 2014-01--2017-12
（ 9 ） 海水鱼类原始生殖细胞生成模式及其分离和冷冻保存研究, 主持, 省级, 2012-01--2014-09
（ 10 ） “十二五”国家863计划“海洋生物细胞分子育种关键技术”, 参与, 国家级, 2012-01--2015-12
（ 11 ） 海水养殖种质改良技术和产业化示范, 参与, 部委级, 2012-01--2014-12
（ 12 ） 鲆鲽鱼类精子成熟及其超活化获能机制研究, 主持, 国家级, 2016-01--2019-12
（ 13 ） 大西洋鲑鱼种高效扩繁技术工艺与示范, 参与, 省级, 2018-01--2020-12
（ 14 ） 重要养殖鱼类生殖干细胞诱导与移植技术, 主持, 国家级, 2018-12--2022-12
（ 15 ） 海水鱼类精原干细胞移植即“借腹生子技术”研发, 主持, 省级, 2019-01--2020-12
（ 16 ） 蓝色生命前前技术, 参与, 省级, 2019-01--2020-12
（ 17 ） 海水鱼类“借腹生子”远缘精原干细胞在受体早期定植障碍解析, 主持, 省级, 2021-01--2023-12

（1）基于vasa/dnd基因标记的两种海水鱼原始生殖细胞发生发育的究   基于vasa/dnd基因标记的两种海水鱼原始生殖细胞发生发育的究   刘清华 林帆 李军   2013-11-01
（2）Marine fish genetic resources cryoperservation and application in breeding   刘清华   2013-08-23

已指导学生

赵海霞  硕士研究生  085238-生物工程