以假结核耶尔森氏菌(Yersinia pseudotuberculosis)、结核分枝杆菌(Mycobacterium tuberculosis)等重要病原细菌为研究对象，对病原细菌重要基因的表达和功能调控及机理进行探讨，揭示病原细菌毒力与感染、潜伏与耐药、压力耐受等调控机理，寻找新的药物作用靶点。

071005-微生物学

病原微生物

   

2018-11~现在, 中国科学院武汉病毒研究所, 青年研究员
2012-03~2018-10,中国科学院武汉病毒研究所, 副研究员
2011-06~2012-02,法国CNRS CPBS, 博士后

微生物前沿
科技论文阅读与写作
科技文献阅读
微生物学

   

（1） 湖北省优秀博士学位论文, , 省级, 2012
（2） 中国科学院院长优秀奖, , 院级, 2011
（3） 中国科学院优秀毕业生, , 院级, 2011

[1] 胡杨波, 何丽娜, 陈士云. 细菌基因组绝缘位点的鉴定方法及应用. CN: CN114134091A, 2022-03-04.
[2] 胡杨波, 冯立鹏, 陈士云. 一种无背景定向克隆PCR产物的载体及制备方法和应用. CN: CN102628057A, 2012-08-08.

   

[1] Huang, Shaojia, Zhou, Wei, Tang, Wei, Zhang, Yong, Hu, Yangbo, Chen, Shiyun. Genome-scale analyses of transcriptional start sites in Mycobacterium marinum under normoxic and hypoxic conditions. BMC GENOMICS[J]. 2021, 22(1): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022548/.
[2] Zhang, Baoyue, Yu, Bing, Zhou, Wei, Wang, Yue, Sun, Ziyong, Wu, Xiaojun, Chen, Shiyun, Ni, Ming, Hu, Yangbo. Mobile Plasmid Mediated Transition From Colistin-Sensitive to Resistant Phenotype in Klebsiella pneumoniae. FRONTIERS IN MICROBIOLOGY[J]. 2021, 12: https://doaj.org/article/6adf036ea9bb452aaca6056f1dba7f24.
[3] Tu, Yunchuan, Tang, Wei, Yu, Liang, Liu, Zheyi, Liu, Yanting, Xia, Huicong, Zhang, Haiwei, Chen, Shiyun, Wu, Jia, Cui, Xiaoju, Zhang, Jianan, Wang, Fangjun, Hu, Yangbo, Deng, Dehui. Inactivating SARS-CoV-2 by electrochemical oxidation. SCIENCE BULLETIN[J]. 2021, 66(7): 720-726, https://www.sciengine.com/doi/10.1016/j.scib.2020.12.025.
[4] Yang Yang, Chang Liu, Wei Zhou, Wei Shi, Ming Chen, Baoyue Zhang, David G Schatz, Yangbo Hu, Bin Liu. Structural visualization of transcription activated by a multidrug-sensing MerR family regulator. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-22990-8.
[5] Shi, Wei, Zhang, Baoyue, Jiang, Yanan, Liu, Chang, Zhou, Wei, Chen, Ming, Yang, Yang, Hu, Yangbo, Liu, Bin. Structural basis of copper-efflux-regulator-dependent transcription activation. ISCIENCE[J]. 2021, 24(5): https://doaj.org/article/638e041555fb449ebff6976a398ee4a5.
[6] Li, Gang, Zhao, Qian, Luan, Tian, Hu, Yangbo, Zhang, Yueling, Li, Ting, Wang, Chunlai, Xie, Fang, Zhang, Wanjiang, Langford, Paul R, Liu, Siguo. Basal-Level Effects of (p)ppGpp in the Absence of Branched-Chain Amino Acids in Actinobacillus pleuropneumoniae. JOURNAL OF BACTERIOLOGY[J]. 2020, 202(8): [7] Shi, Wei, Zhou, Wei, Zhang, Baoyue, Huang, Shaojia, Jiang, Yanan, Schammel, Abigail, Hu, Yangbo, Liu, Bin. Structural basis of bacterial sigma(28)-mediated transcription reveals roles of theRNApolymerase zinc-binding domain. EMBO JOURNAL[J]. 2020, 39(14): http://dx.doi.org/10.15252/embj.2020104389.
[8] Wei Shi, Wei Zhou, Baoyue Zhang, Shaojia Huang, Yanan Jiang, Abigail Schammel, Yangbo Hu, Bin Liu. Structural basis of bacterial σ 28 ‐mediated transcription reveals roles of the RNA polymerase zinc‐binding domain. THE EMBO JOURNAL. 2020, 39(14): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360974/.
[9] Mao, Chunyou, Zhu, Yan, Lu, Pei, Feng, Lipeng, Chen, Shiyun, Hu, Yangbo. Association of omega with the C-Terminal Region of the beta' Subunit Is Essential for Assembly of RNA Polymerase in Mycobacterium tuberculosis. JOURNAL OF BACTERIOLOGY[J]. 2018, 200(12): http://202.127.146.157/handle/2RYDP1HH/5275.
[10] Perumal, Ayyappasamy Sudalaiyadum, Vishwakarma, Rishi Kishore, Hu, Yangbo, Morichaud, Zakia, Brodolin, Konstantin. RbpA relaxes promoter selectivity of M-tuberculosis RNA polymerase. NUCLEIC ACIDS RESEARCH[J]. 2018, 46(19): 10106-10118, http://www.corc.org.cn/handle/1471x/2373223.
[11] 陈士云. Association of ω with the C-terminal region of β' subunit is essential for assembly of RNA polymerase in Mycobacterium tuberculosis.. J Bacteriol. 2018, [12] Lu, Pei, Wang, Yifei, Hu, Yangbo, Chen, Shiyun. RgsA, an RpoS-dependent sRNA, negatively regulates rpoS expression in Pseudomonas aeruginosa. MICROBIOLOGY-SGM[J]. 2018, 164(4): 716-724, https://www.webofscience.com/wos/woscc/full-record/WOS:000438758300027.
[13] Feng, Lipeng, Chen, Shiyun, Hu, Yangbo. PhoPR Positively Regulates whiB3 Expression in Response to Low pH in Pathogenic Mycobacteria. JOURNAL OF BACTERIOLOGY[J]. 2018, 200(8): http://202.127.146.157/handle/2RYDP1HH/5059.
[14] Wang, Zhongwei, Cumming, Bridgette M, Mao, Chunyou, Zhu, Yan, Lu, Pei, Steyn, Adrie J C, Chen, Shiyun, Hu, Yangbo. RbpA and sigma(B) association regulates polyphosphate levels to modulate mycobacterial isoniazid-tolerance. MOLECULAR MICROBIOLOGY[J]. 2018, 108(6): 627-640, http://202.127.146.157/handle/2RYDP1HH/5380.
[15] Yixiang Liu, Joshua Rose, Shaojia Huang, Yangbo Hu, Qiong Wu, Dan Wang, Conggang Li, Maili Liu, Pei Zhou, Ling Jiang. A pH-gated conformational switch regulates the phosphatase activity of bifunctional HisKA-family histidine kinases. NATURE COMMUNICATIONS[J]. 2017, 8(1): http://dx.doi.org/10.1038/s41467-017-02310-9.
[16] Zhu, Yan, Mao, Chunyou, Ge, Xingyi, Wang, Zhongwei, Lu, Pei, Zhang, Yong, Chen, Shiyun, Hu, Yangbo. Characterization of a Minimal Type of Promoter Containing the-10 Element and a Guanine at the-14 or-13 Position in Mycobacteria. JOURNAL OF BACTERIOLOGY[J]. 2017, 199(21): http://www.corc.org.cn/handle/1471x/2373395.
[17] Yang, ShanShan, Hu, YangBo, Wang, XuDe, Gao, YunRong, Li, Kun, Zhang, XianEn, Chen, ShiYun, Zhang, TianYu, Gu, Ling, Deng, JiaoYu. Deletion of sigB Causes Increased Sensitivity to para-Aminosalicylic Acid and Sulfamethoxazole in Mycobacterium tuberculosis. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY[J]. 2017, 61(10): http://www.corc.org.cn/handle/1471x/2373386.
[18] Chen, Zhenkang, Hu, Yangbo, Cumming, Bridgette M, Lu, Pei, Feng, Lipeng, Deng, Jiaoyu, Steyn, Adrie J C, Chen, Shiyun. Mycobacterial WhiB6 Differentially Regulates ESX-1 and the Dos Regulon to Modulate Granuloma Formation and Virulence in Zebrafish. CELL REPORTS[J]. 2016, 16(9): 2512-2524, http://www.corc.org.cn/handle/1471x/2375337.
[19] Feng, Lipeng, Chen, Zhenkang, Wang, Zhongwei, Hu, Yangbo, Chen, Shiyun. Genome-wide characterization of monomeric transcriptional regulators in Mycobacterium tuberculosis. MICROBIOLOGY-SGM[J]. 2016, 162: 889-897, https://www.webofscience.com/wos/woscc/full-record/WOS:000377148700017.
[20] 陈士云. σE-dependent activation of RbpA controls transcription of the furA-katG operon in response to oxidative stress in mycobacteria. Mol Microbiol. 2016, [21] Lu, Pei, Wang, Yifei, Zhang, Yong, Hu, Yangbo, Thompson, Karl M, Chen, Shiyun. RpoS-dependent sRNA RgsA regulates Fis and AcpP in Pseudomonas aeruginosa. MOLECULAR MICROBIOLOGY[J]. 2016, 102(2): 244-259, https://www.webofscience.com/wos/woscc/full-record/WOS:000386101000006.
[22] Hu, Yangbo, Wang, Zhongwei, Feng, Lipeng, Chen, Zhenkang, Mao, Chunyou, Zhu, Yan, Chen, Shiyun. sigma(E)-dependent activation of RbpA controls transcription of the furA-katG operon in response to oxidative stress in mycobacteria. MOLECULAR MICROBIOLOGY[J]. 2016, 102(1): 107-120, http://www.corc.org.cn/handle/1471x/2375051.
[23] Li, Yunlong, Hu, Yangbo, Francis, Matthew S, Chen, Shiyun. RcsB positively regulates the Yersinia Ysc-Yop type III secretion system by activating expression of the master transcriptional regulator LcrF. ENVIRONMENTAL MICROBIOLOGY[J]. 2015, 17(4): 1219-1233, https://www.webofscience.com/wos/woscc/full-record/WOS:000352545100023.
[24] Yangbo Hu, Zakia Morichaud, Ayyappasamy SudalaiyadumPerumal, Franoise RoquetBaneres, Konstantin Brodolin. Mycobacterium RbpA cooperates with the stress-response σ B subunit of RNA polymerase in promoter DNA unwinding. NUCLEIC ACIDS RESEARCH. 2014, 42(16): 10399-10408, [25] Li, Lamei, Yan, Huan, Feng, Lipeng, Li, Yunlong, Lu, Pei, Hu, Yangbo, Chen, Shiyun. LcrQ Blocks the Role of LcrF in Regulating the Ysc-Yop Type III Secretion Genes in Yersinia pseudotuberculosis. PLOS ONE[J]. 2014, 9(3): https://doaj.org/article/f3e416af07ed4b7bb2ba133790fbcea3.
[26] Lu, Pei, Zhang, Yong, Hu, Yangbo, Francis, Matthew S, Chen, Shiyun. A cis-encoded sRNA controls the expression of fabH2 in Yersinia. FEBS LETTERS[J]. 2014, 588(10): 1961-1966, http://dx.doi.org/10.1016/j.febslet.2014.04.005.
[27] Li, Yunlong, Li, Lamei, Huang, Li, Francis, Matthew S, Hu, Yangbo, Chen, Shiyun. Yersinia Ysc-Yop type III secretion feedback inhibition is relieved through YscV-dependent recognition and secretion of LcrQ. MOLECULAR MICROBIOLOGY[J]. 2014, 91(3): 494-507, https://www.webofscience.com/wos/woscc/full-record/WOS:000345498000005.
[28] Lu, Pei, Zhang, Yong, Li, Lamei, Hu, Yangbo, Huang, Li, Li, Yunlong, Rayner, Simon, Chen, Shiyun. Small non-coding RNA SraG regulates the operon YPK_1206-1205 in Yersinia pseudotuberculosis. FEMS MICROBIOLOGY LETTERS[J]. 2012, 331(1): 37-43, https://www.webofscience.com/wos/woscc/full-record/WOS:000303438800006.
[29] Hu, Yangbo, Morichaud, Zakia, Chen, Shiyun, Leonetti, JeanPaul, Brodolin, Konstantin. Mycobacterium tuberculosis RbpA protein is a new type of transcriptional activator that stabilizes the Sigma(A)-containing RNA polymerase holoenzyme. NUCLEIC ACIDS RESEARCH[J]. 2012, 40(14): 6547-6557, http://dx.doi.org/10.1093/nar/gks346.
[30] Yangbo Hu, Zakia Morichaud, Shiyun Chen, JeanPaul Leonetti, Konstantin Brodolin. Mycobacterium tuberculosis RbpA protein is a new type of transcriptional activator that stabilizes the σ A -containing RNA polymerase holoenzyme. NUCLEIC ACIDS RESEARCH. 2012, 40(14): 6547-6557, [31] Hu, Yangbo, Feng, Lipeng, Li, Yunlong, Zhang, Yong, Lu, Pei, Rayner, Simon, Chen, Shiyun. Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions. PLOS ONE[J]. 2012, 7(11): http://dx.doi.org/10.1371/journal.pone.0050142.
[32] Hu, Yangbo, Lu, Pei, Zhang, Yong, Li, Yunlong, Li, Lamei, Huang, Li, Chen, Shiyun. Cra negatively regulates acid survival in Yersinia pseudotuberculosis. FEMS MICROBIOLOGY LETTERS[J]. 2011, 317(2): 190-195, http://www.corc.org.cn/handle/1471x/2376022.
[33] Zhang, Yong, Hu, Yangbo, Yang, Baoyu, Ma, Fang, Lu, Pei, Li, Lamei, Wan, Chengsong, Rayner, Simon, Chen, Shiyun. Duckweed (Lemna minor) as a Model Plant System for the Study of Human Microbial Pathogenesis. PLOS ONE[J]. 2010, 5(10): http://www.corc.org.cn/handle/1471x/2375711.
[34] Hu, Yangbo, Lu, Pei, Zhang, Yong, Li, Lamei, Chen, Shiyun. Characterization of an aspartate-dependent acid survival system in Yersinia pseudotuberculosis. FEBS LETTERS[J]. 2010, 584(11): 2311-2314, http://dx.doi.org/10.1016/j.febslet.2010.03.045.
[35] Hu, Yangbo, Wang, Yao, Ding, Lisha, Lu, Pei, Atkinson, Steve, Chen, Shiyun. Positive regulation of flhDC expression by OmpR in Yersinia pseudotuberculosis. MICROBIOLOGY-SGM[J]. 2009, 155: 3622-3631, http://www.corc.org.cn/handle/1471x/2375558.
[36] Hu, Yangbo, Lu, Pei, Wang, Yao, Ding, Lisha, Atkinson, Steve, Chen, Shiyun. OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis. MICROBIOLOGY-SGM[J]. 2009, 155: 2522-2531, http://www.corc.org.cn/handle/1471x/2375546.
[37] Ding, Lisha, Wang, Yao, Hu, Yangbo, Atkinson, Steve, Williams, Paul, Chen, Shiyun. Functional characterization of FlgM in the regulation of flagellar synthesis and motility in Yersinia pseudotuberculosis. MICROBIOLOGY-SGM[J]. 2009, 155: 1890-1900, http://www.corc.org.cn/handle/1471x/2375560.
[38] 王瑶, 丁莉莎, 胡杨波, 张勇, 杨宝玉, 陈士云. 假结核耶尔森氏菌flhDC基因影响细菌运动性和生物膜形成. 中国科学：C辑[J]. 2007, 37(5): 516-523, http://lib.cqvip.com/Qikan/Article/Detail?id=25646118.
[39] 王瑶, 戴岳, 张勇, 胡杨波, 杨宝玉, 陈士云. 群体感应信号分子降解基因对铜绿假单胞菌毒力和生物膜形成的影响. 中国科学：C辑[J]. 2007, 37(2): 234-240, http://lib.cqvip.com/Qikan/Article/Detail?id=24394865.
[40] Yao, Wang, Lisha, Ding, YangBo, Hu, Yong, Zhang, BaoYu, Yang, ShiYun, Chen. The flhDC gene affects motility and biofilm formation in Yersinia pseudotuberculosis (Retracted article. See vol. 52, pg. 796, 2009). SCIENCE IN CHINA SERIES C-LIFE SCIENCES[J]. 2007, 50(6): 814-821, http://www.corc.org.cn/handle/1471x/2375344.
[41] WANG Yao, DING LiSha, HU YangBo, ZHANG Yong, YANG BaoYu, CHEN ShiYun. The flhDC gene affects motility and biofilm formation in Yersinia pseudotuberculosis. SCIENCE IN CHINA. SERIES C: LIFE SCIENCE[J]. 2007, 50(6): 814-821, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=3192908&detailType=1.
[42] Wang Yao, Dai Yue, Zhang Yong, Hu YangBo, Yang BaoYu, Chen ShiYun. Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of Pseudomonas aeruginosa. SCIENCE IN CHINA SERIES C-LIFE SCIENCES[J]. 2007, 50(3): 385-391, http://www.corc.org.cn/handle/1471x/2375421.
[43] 王瑶, 戴岳, 张勇, 胡杨波, 杨宝玉, 陈士云. 群体感应信号分子降解基因对铜绿假单胞菌毒力和生物膜形成的影响. 中国科学：C辑[J]. 2007, 37(2): 234-240, http://lib.cqvip.com/Qikan/Article/Detail?id=24394865.

   

（ 1 ） 结核分枝杆菌RbpA 转录调控及在利福平耐药中的功能研究, 主持, 国家级, 2013-01--2015-12
（ 2 ） 结核分枝杆菌感染过程中核糖体基因转录调控研究, 主持, 国家级, 2013-01--2016-12
（ 3 ） 青年创新促进会, 主持, 部委级, 2013-01--2016-12
（ 4 ） 结核分枝杆菌“GG”启动子新元件鉴定及参与转录起始的分子机制研究, 主持, 国家级, 2017-01--2020-12
（ 5 ） 青促会优秀会员, 主持, 部委级, 2018-01--2021-12

已指导学生

张柳娣  硕士研究生  071005-微生物学  

朱艳  硕士研究生  085238-生物工程  

现指导学生

张宝月  硕士研究生  085238-生物工程  

费可可  博士研究生  100103-病原生物学  

黄绍佳  博士研究生  071005-微生物学  

周卫  博士研究生  071005-微生物学  

陈鸣  硕士研究生  085238-生物工程  

何丽娜  硕士研究生  071005-微生物学