071007-遗传学

重要生物制剂的生物合成和代谢调控
微生物代谢工程和系统生物学
合成生物学的新方法和新技术

2005-09--2008-06   中国科学院微生物研究所   博士
2002-09--2005-06   中国农业大学    硕士
1998-09--2002-06   中国农业大学   学士

   

2008-07~现在, 中国科学院微生物研究所, 博士

   

（1） L-苏氨酸，L-蛋氨酸和L-丝氨酸基因工程菌构建及发酵条件优化, 市地级, 2016
（2） 赖氨酸和苏氨酸工业生产菌改造的关键技术与产业化应用, 二等奖, 部委级, 2014

（ 1 ） 一种产L-氨基酸的重组菌、其构建方法及L-氨基酸生产方法, 发明专利, 2015, 第 3 作者, 专利号: PCT/CN2014/070228
（ 2 ） 一种催化生产1, 5-戊二胺的工程菌及其应用, 发明专利, 2014, 第 4 作者, 专利号: 201410302561.7
（ 3 ） 生产L-组氨酸的方法及其专用重组菌, 发明专利, 2014, 第 3 作者, 专利号: 201410050868.2
（ 4 ） 一种阿拉伯糖诱导的表达载体及其构建方法和应用, 发明专利, 2013, 第 2 作者, 专利号: ZL201210139353.0
（ 5 ） 一种生产L-2-氨基丁酸的方法及其专用重组菌, 发明专利, 2015, 第 4 作者, 专利号: 201510208576.1
（ 6 ） 一种提高谷氨酸棒杆菌氨基酸产量的方法及其应用, 发明专利, 2016, 第 3 作者, 专利号: CN201610546762.0
（ 7 ） 苏氨酸衰减子的突变体及其应用以及解除苏氨酸操纵子反馈阻遏的方法, 发明专利, 2016, 第 4 作者, 专利号: CN201611035232.6
（ 8 ） 一种5-UTR元件及其在生产中的应用, 发明专利, 2016, 第 4 作者, 专利号: CN201610987080.3
（ 9 ） 産L-アミノ酸の組換え菌およびその構築方法, 发明专利, 2017, 第 3 作者, 专利号: JP6188930
（ 10 ） constructing method therefor and method for producing L-amino acid, 发明专利, 2017, 第 3 作者, 专利号: US9,796,991
（ 11 ） DNA分子、表达载体、重组菌及其构建方法，以及羟脯氨酸的制备方法, 发明专利, 2017, 第 3 作者, 专利号: 201711165839.0
（ 12 ） 生产L-丝氨酸的重组菌及其构建方法, 发明专利, 2017, 第 1 作者, 专利号: 201711146433.8
（ 13 ） Recombinant strain producing L-amino acid, constructing method therefor and method for producing L-amino acid, 发明专利, 2018, 第 3 作者, 专利号: KR10-2015-7032480
（ 14 ） 生产L-组氨酸的重组菌、其构建方法以及L-组氨酸的生产方法, 发明专利, 2018, 第 3 作者, 专利号: 201810118782.7
（ 15 ） 生产L-亮氨酸的重组菌、其构建方法及L-亮氨酸的生产方法, 发明专利, 2018, 第 3 作者, 专利号: 201810367158.0
（ 16 ） 全细胞催化生产5-氨基戊酸的工程菌及5-氨基戊酸的制备方法, 发明专利, 2019, 第 3 作者, 专利号: 201910342666.8

   

[1] 张芸, 刘喆, 王雪亮, 菅慧, 肖海函, 温廷益. SHMT2 promotes cell viability and inhibits ROS-dependent, mitochondrial-mediated apoptosis via the intrinsic signaling pathway in bladder cancer cells. Cancer Gene Therapy[J]. 2022, [2] Yun Zhang, Zhao, Jing, Xueliang Wang, Yuan Tang, Shuwen Liu, 温廷益. Model-Guided Metabolic Rewiring for Gamma-Aminobutyric Acid and Butyrolactam Biosynthesis in Corynebacterium glutamicum ATCC13032. Biology[J]. 2022, https://www.mdpi.com/2079-7737/11/6/846.
[3] 菅慧, 张芸, 王珺玥, 陈振翔, 温廷益. Zeolitic imidazolate framework-based nanoparticles for the cascade enhancement of cancer chemodynamic therapy by targeting glutamine metabolism. Nanoscale[J]. 2022, [4] Haihan Xiao, Haijuan Xiao, Yun Zhang, Lingyun Guo, Zhenzhen Dou, Wenya Feng, Linlin Liu, Bing Liu, Tianming Chen, Gang Liu, 温廷益. High-throughput sequencing unravels the cell heterogeneity of cerebrospinal fluid in the bacterial meningitis of children. Frontiers in Immunology[J]. 2022, [5] Lai Li, Aihua Deng, Shuwen Liu, Junyue Wang, Ruilin Shi, Tiantian Wang, cui di, hua bai, Yun Zhang, 温廷益. A Universal Method for Developing Autoinduction Expression Systems Using AHL-Mediated Quorum-Sensing Circuits. ACS SYNTHETIC BIOLOGY[J]. 2022, [6] 张芸. 整合组学的代谢网络模型研究进展. 科学通报[J]. 2021, 66(19): 2393-2404, [7] 张芸. 2.Molecular basis for feedback inhibition of tyrosine-regulated 3-deoxy-Darabino- heptulosonate-7-phosphate synthase from Escherichia coli. Journal of Structural Biology. 2019, [8] 张宇, 张芸, 王博, 商秀玲, 胡启跳, 刘树文, 温廷益. Reconstruction of TCA cycle in Corynebacterium glutamicum with a genome-scale metabolic network model for trans-4-hydroxyproline production. Biotechnology and Bioengineering[J]. 2019, [9] Liu, Shuwen, Xiao, Haihan, Zhang, Fangfang, Lu, Zheng, Zhang, Yun, Deng, Aihua, Li, Zhongcai, Yang, Cui, Wen, Tingyi. A seamless and iterative DNA assembly method named PS-Brick and its assisted metabolic engineering for threonine and 1-propanol production. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 12(1): http://gooa.las.ac.cn/external/index?type=-1&pid=1507328.
[10] Wu, Jie, Deng, Aihua, Sun, Qinyun, Bai, Hua, Sun, Zhaopeng, Shang, Xiuling, Zhang, Yun, Liu, Qan, Liang, Yong, Liu, Shuwen, Che, Yongsheng, Wen, Tingyi. Bacterial Genome Editing via a Designed Toxin-Antitoxin Cassette. ACS SYNTHETIC BIOLOGY[J]. 2018, 7(3): 822-831, https://www.webofscience.com/wos/woscc/full-record/WOS:000428003700010.
[11] Wang, Laiyou, Deng, Aihua, Zhang, Yun, Liu, Shuwen, Liang, Yong, Bai, Hua, Cui, Di, Qiu, Qidi, Shang, Xiuling, Yang, Zhao, He, Xiuping, Wen, Tingyi. Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts. BIOTECHNOLOGY FOR BIOFUELS[J]. 2018, 11(1): https://doaj.org/article/89f38284172242c5885d1c25bac97cc6.
[12] Wang, Bo, Hu, Qitiao, Zhang, Yu, Shi, Ruilin, Chai, Xin, Liu, Zhe, Shang, Xiuling, Zhang, Yun, Wen, Tingyi. A RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum. MICROBIAL CELL FACTORIES[J]. 2018, 17(1): https://doaj.org/article/3b9c3e92787141d6a9d0d98e070ab911.
[13] Bai, Hua, Deng, Aihua, Liu, Shuwen, Cui, Di, Qiu, Qidi, Wang, Laiyou, Yang, Zhao, Wu, Jie, Shang, Xiuling, Zhang, Yun, Wen, Tingyi. A Novel Tool for Microbial Genome Editing Using the Restriction-Modification System. ACS SYNTHETIC BIOLOGY[J]. 2018, 7(1): 98-106, https://www.webofscience.com/wos/woscc/full-record/WOS:000423252800012.
[14] Zhang, Yun, Shang, Xiuling, Lai, Shujuan, Zhang, Yu, Hu, Qitiao, Chai, Xin, Wang, Bo, Liu, Shuwen, We, Tingyi. Reprogramming One-Carbon Metabolic Pathways To Decouple L-Serine Catabolism from Cell Growth in Corynebacterium glutamicum. ACS SYNTHETIC BIOLOGY[J]. 2018, 7(2): 635-646, https://www.webofscience.com/wos/woscc/full-record/WOS:000426012600034.
[15] Shang, Xiuling, Chai, Xin, Lu, Xuemei, Li, Yuan, Zhang, Yun, Wang, Guoqiang, Zhang, Chen, Liu, Shuwen, Zhang, Yu, Ma, Jiyin, Wen, Tingyi. Native promoters of Corynebacterium glutamicum and its application in l-lysine production. BIOTECHNOLOGY LETTERS[J]. 2018, 40(2): 383-391, https://www.webofscience.com/wos/woscc/full-record/WOS:000425115100020.
[16] Yang, Zhao, He, Luyun, Lin, Kaisu, Zhang, Yun, Deng, Aihua, Liang, Yong, Li, Chong, Wen, Tingyi. The KMT1A-GATA3-STAT3 Circuit Is a Novel Self-Renewal Signaling of Human Bladder Cancer Stem Cells. CLINICAL CANCER RESEARCH[J]. 2017, 23(21): 6673-6685, http://dx.doi.org/10.1158/1078-0432.CCR-17-0882.
[17] 张芸. A Novel Tool for Microbial Genome Editing Using the Restriction-Modification System.. ACS synthetic Biology. 2017, [18] Zhang, Yu, Cai, Jingyi, Shang, Xiuling, Wang, Bo, Liu, Shuwen, Chai, Xin, Tan, Tianwei, Zhang, Yun, Wen, Tingyi. A new genome-scale metabolic model of Corynebacterium glutamicum and its application. BIOTECHNOLOGY FOR BIOFUELS[J]. 2017, 10(1): http://dx.doi.org/10.1186/s13068-017-0856-3.
[19] Chai, Xin, Shang, Xiuling, Zhang, Yu, Liu, Shuwen, Liang, Yong, Zhang, Yun, Wen, Tingyi. A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum. BMC BIOTECHNOLOGY[J]. 2016, 16(1): http://dx.doi.org/10.1186/s12896-016-0313-6.
[20] Liu, Qian, Liang, Yong, Zhang, Yun, Shang, Xiuling, Liu, Shuwen, Wen, Jifu, Wen, Tingyi. YjeH Is a Novel Exporter of L-Methionine and Branched-Chain Amino Acids in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2015, 81(22): 7753-7766, http://dx.doi.org/10.1128/AEM.02242-15.
[21] 张芸. Benzoate metabolism intermediate benzoyl coenzyme affects gentisate pathway regulation in Comamonas testosteroni.. 2014, [22] Chen, DongWei, Zhang, Yun, Jiang, ChengYing, Liu, ShuangJiang. Benzoate Metabolism Intermediate Benzoyl Coenzyme A Affects Gentisate Pathway Regulation in Comamonas testosteroni. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2014, 80(13): 4051-4062, https://www.webofscience.com/wos/woscc/full-record/WOS:000337241400029.
[23] Ni, Bin, Zhang, Yun, Chen, DongWei, Wang, BaoJun, Liu, ShuangJiang. Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5-cleavage pathway in bacteria. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2013, 97(13): 6031-6041, https://www.webofscience.com/wos/woscc/full-record/WOS:000321121500032.
[24] Shang, Xiuling, Zhang, Yun, Zhang, Guoqiang, Chai, Xin, Deng, Aihua, Liang, Yong, Wen, Tingyi. Characterization and Molecular Mechanism of AroP as an Aromatic Amino Acid and Histidine Transporter in Corynebacterium glutamicum. JOURNAL OF BACTERIOLOGY[J]. 2013, 195(23): 5334-5342, http://dx.doi.org/10.1128/JB.00971-13.
[25] Lai ShuJuan, Zhang Yun, Liu ShuWen, Liang Yong, Shang XiuLing, Chai Xin, Wen TingYi. Metabolic engineering and flux analysis of for Corynebacterium glutamicum for L-serine production. SCIENCE CHINA-LIFE SCIENCES[J]. 2012, 55(4): 283-290, http://dx.doi.org/10.1007/s11427-012-4304-0.
[26] Zhang, Guoqiang, Wang, Wenzhao, Deng, Aihua, Sun, Zhaopeng, Zhang, Yun, Liang, Yong, Che, Yongsheng, Wen, Tingyi. A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria. PLOS GENETICS[J]. 2012, 8(9): http://dx.doi.org/10.1371/journal.pgen.1002987.
[27] Zhang, Yun, Shang, Xiuling, Lai, Shujuan, Zhang, Guoqiang, Liang, Yong, Wen, Tingyi. Development and Application of an Arabinose-Inducible Expression System by Facilitating Inducer Uptake in Corynebacterium glutamicum. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2012, 78(16): 5831-5838, http://dx.doi.org/10.1128/AEM.01147-12.
[28] Lai Shujuan, Zhang Yun, Liu Shuwen, Liang Yong, Shang Xiuling, Chai Xin, Wen Tingyi. Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production. 中国科学：生命科学英文版[J]. 2012, 55(4): 283-290, http://lib.cqvip.com/Qikan/Article/Detail?id=41802789.
[29] LAI ShuJuan, ZHANG Yun, LIU ShuWen, LIANG Yong, SHANG XiuLing, CHAI Xin, WEN TingYi. Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production. 中国科学：生命科学英文版[J]. 2012, 55(4): 283-290, http://lib.cqvip.com/Qikan/Article/Detail?id=41802789.
[30] Zhang, Yun, Shang, Xiuling, Deng, Aihua, Chai, Xin, Lai, Shujuan, Zhang, Guoqiang, Wen, Tingyi. Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine. BIOCHIMIE[J]. 2012, 94(3): 829-838, http://dx.doi.org/10.1016/j.biochi.2011.11.015.
[31] Zhang, Guoqiang, Bao, Peng, Zhang, Yun, Deng, Aihua, Chen, Ning, Wen, Tingyi. Enhancing electro-transformation competency of recalcitrant Bacillus amyloliquefaciens by combining cell-wall weakening and cell-membrane fluidity disturbing. ANALYTICAL BIOCHEMISTRY[J]. 2011, 409(1): 130-137, http://dx.doi.org/10.1016/j.ab.2010.10.013.
[32] 闫继爱, 张雪, 张芸, 左佃光, 陈宁, 温廷益. 利用Red同源重组技术构建产L-苏氨酸的基因工程菌. 中国生物工程杂志[J]. 2010, 79-84, http://lib.cqvip.com/Qikan/Article/Detail?id=33626542.
[33] Deng, Aihua, Wu, Jie, Zhang, Yun, Zhang, Guoqiang, Wen, Tingyi. Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp B001. BIORESOURCE TECHNOLOGY[J]. 2010, 101(18): 7100-7106, http://dx.doi.org/10.1016/j.biortech.2010.03.130.
[34] Zhang, Yun, Wu, JianFeng, Zeyer, Josef, Meng, Bo, Liu, Lei, Jiang, ChengYing, Liu, SiQi, Liu, ShuangJiang. Proteomic and molecular investigation on the physiological adaptation of Comamonas sp strain CNB-1 growing on 4-chloronitrobenzene. BIODEGRADATION[J]. 2009, 20(1): 55-66, https://www.webofscience.com/wos/woscc/full-record/WOS:000262085700006.
[35] 张雪, 闫继爱, 于雷, 张国强, 张芸, 陈宁, 温廷益. 含苏氨酸操纵子重组质粒的构建及其对大肠杆菌L-苏氨酸积累的影响. 微生物学报[J]. 2009, 591-596, http://lib.cqvip.com/Qikan/Article/Detail?id=30260895.
[36] 张芸. The complete genome of Comamonas testosteroni reveals its evolutionary and genetic adaptations to the changing environments.. 2009, [37] 张芸. 丛毛单胞菌降解芳烃化合物及对砷酸盐抗性的. 2008, 146-, http://124.16.144.217/handle/311023/3644.
[38] Zhang, Yun, Ma, YingFei, Qi, SuWei, Meng, Bo, Chaudhry, Muhammad Tausif, Liu, SiQi, Liu, ShuangJiang. Responses to arsenate stress by Comamonas sp strain CNB-1 at genetic and proteomic levels. MICROBIOLOGY-SGM[J]. 2007, 153: 3713-3721, http://124.16.144.217/handle/311023/1780.
[39] Qi, SuWei, Chaudhry, Muhammad Tausif, Zhang, Yun, Meng, Bo, Huang, Yan, Zhao, KX, Poetsch, Ansgar, Jiang, ChengYing, Liu, Siqi, Liu, ShuangJiang. Comparative proteomes of Corynebacterium glutamicum grown on aromatic compounds revealed novel proteins involved in aromatic degradation and a clear link between aromatic catabolism and gluconeogenesis via fructose-1,6-bisphosphatase. PROTEOMICS[J]. 2007, 7(20): 3775-3787, https://www.webofscience.com/wos/woscc/full-record/WOS:000250641500014.

   

（ 1 ） 组氨酸工程菌发酵关键技术研究与生产示范, 主持, 部委级, 2014-03--2015-12
（ 2 ） 尼龙5X 盐关键生产技术及产业化, 参与, 部委级, 2017-01--2018-12
（ 3 ） 5-氨基戊酸合成功能模块的组装及人工生物系统构建, 主持, 国家级, 2019-01--2020-12

（1）基因组规模代谢网络模型指导的代谢工程研究   2017氨基酸国际产业高峰论坛   2017-12-05
（2）基因组规模代谢网络模型指导的氨基酸代谢工程改造   中国生物工程学会第11届学术年会暨2017年全国生物技术大会   2017-09-24

已指导学生

刘喆  硕士研究生  085238-生物工程  

现指导学生

唐源  硕士研究生  071007-遗传学