基本信息
蔡华清  女  博导  中国科学院生物物理研究所
电子邮件: huaqingcai@ibp.ac.cn
通信地址: 北京市朝阳区大屯路15号
邮政编码:

研究领域

细胞巨胞饮的分子机制和生理功能

细胞胞饮,特别是巨胞饮 (macropinocytosis),是细胞摄取营养物质及其它液相大分子的特殊内吞途径,参与免疫反应、病原菌侵袭等重要生物学过程。近年来发现癌细胞可以通过巨胞饮的方式,摄取胞外蛋白质及脂类代谢产物作为营养来源,而阻断胞饮作用可抑制肿瘤生长。作为一种由细胞骨架重排和质膜形变驱动、且伴随大量膜的内化和液体摄入的内吞过程,巨胞饮具有独特的发生机制。此外,巨胞饮过程受到细胞营养状态、环境信号等多种因素的调控,与吞噬和运动等细胞行为之间也存在复杂的调控关系。我们将利用细胞生物学及遗传筛选,系统性鉴定巨胞饮过程的调控基因,阐明它们在胞饮结构起始、形成及成熟过程中的发挥作用的分子机制,并解析这些关键调控基因的病生理功能。

细胞定向运动的分子机制和生理功能

细胞运动是进化上保守的生命活动,是胚胎发育、神经元布线、损伤修复及免疫应答等多种生理活动的基础。细胞迁移的异常又与许多人类重大疾病,特别是癌症的发生发展紧密相关。趋化(chemotaxis)是细胞感应外界信号分子时间和空间梯度的定向运动。利用遗传学、细胞生物学、生物化学等多种手段,结合经典模式生物和动物细胞模型,我们将致力于研究细胞迁移模式的形成基础、方向感应的工作原理和极性建成的调控机制,以期进一步阐明包括肿瘤转移等复杂生物学过程的分子机理。


招生信息

招生专业
071009-细胞生物学
071010-生物化学与分子生物学
招生方向
细胞巨胞饮的分子机质及病生理功能
细胞运动的分子机理及其与疾病的关系

教育背景

2001-09--2007-05   美国耶鲁大学   理学博士
1997-09--2001-07   北京大学   理学学士

工作经历

   
工作简历
2015-09~现在, 中科院生物物理所, 研究组长
2013-07~2015-08,美国约翰霍普金斯大学, 研究助理
2007-10~2013-07,美国约翰霍普金斯大学, 博士后

教授课程

细胞生物学实验-01
科研实践II-科研训练(2022夏)
细胞生物学实验-02
细胞生物学实验
细胞生物学前沿讲座
经典生物文献深度阅读
生命科技经典文献深度研读

出版信息

   
发表论文
[1] Dong Li, Yihong Yang, Chenglin Lv, Yingjie Wang, Xiaoting Chao, Jiafeng Huang, Shashi P. Singh, Ye Yuan, Chengyu Zhang, Jizhong Lou, Pu Gao, Shanjin Huang, Bo Li, Huaqing Cai. GxcM-Fbp17/RacC-WASP signaling regulates polarized cortex assembly in migrating cells via Arp2/3. JOURNAL OF CELL BIOLOGY[J]. 2023, [2] Yang, Yanzhi, Hao, Chen, Du, Jianmei, Xu, Lei, Guo, Zhonglong, Li, Dong, Cai, Huaqing, Guo, Hongwei, Li, Lei. The carboxy terminal transmembrane domain of SPL7 mediates interaction with RAN1 at the endoplasmic reticulum to regulate ethylene signalling in Arabidopsis. NEW PHYTOLOGIST[J]. 2022, 236(3): 878-892, https://www.doi.org/10.1111/nph.18376.
[3] Li, Dong, Sun, Feifei, Yang, Yihong, Tu, Hui, Cai, Huaqing. Gradients of PI(4,5)P-2 and PI(3,5)P-2 Jointly Participate in Shaping the Back State of Dictyostelium Cells. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY[J]. 2022, 10: http://dx.doi.org/10.3389/fcell.2022.835185.
[4] Hadwiger, Jeffrey A, Cai, Huaqing, Aranda, Ramee G, Fatima, Saher. An atypical MAPK regulates translocation of a GATA transcription factor in response to chemoattractant stimulation. JOURNAL OF CELL SCIENCE[J]. 2022, 135(16): [5] Tu, Hui, Wang, Zhimeng, Yuan, Ye, Miao, Xilin, Li, Dong, Guo, Hu, Yang, Yihong, Cai, Huaqing. The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-022-29503-1.
[6] Yiwei Zhang, Hui Tu, Yazhou Hao, Dong Li, Yihong Yang, Ye Yuan, Zhonglong Guo, Lei Li, Haibin Wang, Huaqing Cai. Oligopeptide transporter Slc15A modulates macropinocytosis in Dictyostelium by maintaining intracellular nutrient status. JOURNAL OF CELL SCIENCE[J]. 2022, [7] Yang, Yihong, Li, Dong, Chao, Xiaoting, Singh, Shashi P, Thomason, Peter, Yan, Yonghong, Dong, Mengqiu, Li, Lei, Insall, Robert H, Cai, Huaqing. Leep1 interacts with PIP3 and the Scar/WAVE complex to regulate cell migration and macropinocytosis. JOURNAL OF CELL BIOLOGY[J]. 2021, 220(7): http://dx.doi.org/10.1083/jcb.202010096.
[8] He Zhang, Zhonglong Guo, Yan Zhuang, Yuanzhen Suo, Jianmei Du, Zhaoxu Gao, Jiawei Pan, Li Li, Tianxin Wang, Liang Xiao, Genji Qin, Yuling Jiao, Huaqing Cai, Lei Li. MicroRNA775 regulates intrinsic leaf size and reduces cell wall pectin levels by targeting a galactosyltransferase gene in Arabidopsis. THE PLANT CELL. 2021, 33(3): 581-602, [9] Zhan, Huiwang, Bhattacharya, Sayak, Cai, Huaqing, Iglesias, Pablo A, Huang, ChuanHsiang, Devreotes, Peter N. An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells. DEVELOPMENTALCELL[J]. 2020, 54(5): 608-+, http://dx.doi.org/10.1016/j.devcel.2020.08.001.
[10] Jiao, Zhihua, Cai, Huaqing, Long, Yu, Sirka, Orit Katarina, Padmanaban, Veena, Ewald, Andrew J, Devreotes, Peter N. Statin-induced GGPP depletion blocks macropinocytosis and starves cells with oncogenic defects. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2020, 117(8): 4158-4168, https://www.webofscience.com/wos/woscc/full-record/WOS:000516771500041.
[11] Zhuang, Yan, Zuo, Daqing, Tao, Yihan, Cai, Huaqing, Li, Lei. Laccase3-based extracellular domain provides possible positional information for directing Casparian strip formation in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2020, 117(27): 15400-15402, https://www.webofscience.com/wos/woscc/full-record/WOS:000548351700008.
[12] Edwards, Marc, Cai, Huaqing, AbubakerSharif, Bedri, Long, Yu, Lampert, Thomas J, Devreotes, Peter N. Insight from the maximal activation of the signal transduction excitable network in Dictyostelium discoideum. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2018, 115(16): E3722-E3730, https://www.webofscience.com/wos/woscc/full-record/WOS:000430191900017.
[13] Miao, Yuchuan, Bhattacharya, Sayak, Edwards, Marc, Cai, Huaqing, Inoue, Takanari, Iglesias, Pablo A, Devreotes, Peter N. Altering the threshold of an excitable signal transduction network changes cell migratory modes. NATURE CELL BIOLOGY[J]. 2017, 19(4): 329-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000397917000011.
[14] Senoo, Hiroshi, Cai, Huaqing, Wang, Yu, Sesaki, Hiromi, Iijima, Miho. The novel RacE-binding protein GflB sharpens Ras activity at the leading edge of migrating cells. MOLECULAR BIOLOGY OF THE CELL[J]. 2016, 27(10): 1596-1605, https://www.webofscience.com/wos/woscc/full-record/WOS:000376456800006.
[15] Hoeller, Oliver, Toettcher, Jared E, Cai, Huaqing, Sun, Yaohui, Huang, ChuanHsiang, Freyre, Mariel, Zhao, Min, Devreotes, Peter N, Weiner, Orion D. G beta Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration. PLOS BIOLOGY[J]. 2016, 14(2): https://www.webofscience.com/wos/woscc/full-record/WOS:000371883600012.
[16] Ding, Jingzhen, Segarra, Veronica A, Chen, Shuliang, Cai, Huaqing, Lemmon, Sandra K, FerroNovick, Susan. Auxilin facilitates membrane traffic in the early secretory pathway. MOLECULAR BIOLOGY OF THE CELL[J]. 2016, 27(1): 127-136, https://www.webofscience.com/wos/woscc/full-record/WOS:000367256400011.
[17] Santhanam, Balaji, Cai, Huaqing, Devreotes, Peter N, Shaulsky, Gad, KatohKurasawa, Mariko. The GATA transcription factor GtaC regulates early developmental gene expression dynamics in Dictyostelium. NATURE COMMUNICATIONS[J]. 2015, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000358853600004.
[18] Cai, Huaqing, KatohKurasawa, Mariko, Muramoto, Tetsuya, Santhanam, Balaji, Long, Yu, Li, Lei, Ueda, Masahiro, Iglesias, Pablo A, Shaulsky, Gad, Devreotes, Peter N. Nucleocytoplasmic Shuttling of a GATA Transcription Factor Functions as a Development Timer. SCIENCE[J]. 2014, 343(6177): 1329-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000333108500029.
[19] Huaqing Cai, Peter N. Devreotes. Moving in the right direction: How eukaryotic cells migrate along chemical gradients. SEMINARS IN CELL AND DEVELOPMENTAL BIOLOGY. 2011, 22(8): 834-841, http://dx.doi.org/10.1016/j.semcdb.2011.07.020.
[20] Cai, Huaqing, Das, Satarupa, Kamimura, Yoichiro, Long, Yu, Parent, Carole A, Devreotes, Peter N. Ras-mediated activation of the TORC2-PKB pathway is critical for chemotaxis. JOURNAL OF CELL BIOLOGY[J]. 2010, 190(2): 233-245, https://www.webofscience.com/wos/woscc/full-record/WOS:000280593300009.
[21] 蔡华清. TRAPPI tethers COPII vesicles by binding the coat subunit Sec23p. NATURE. 2007, [22] Cai, Huaqing, Reinisch, Karin, FerroNovick, Susan. Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. DEVELOPMENTAL CELLnull. 2007, 12(5): 671-682, http://dx.doi.org/10.1016/j.devcel.2007.04.005.
[23] 林忠平, 许伟宏, 蔡华清, 孙丽, 胡鸢雷. PEG介导下香菇的转化(英文). 植物学报[J]. 2001, 43(10): 1089-, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=921277&detailType=1.
[24] Cai Huaqing, Zhang Yueyi, Pypaert Marc, Walker Lee, Ferro-Novick Susan. Mutants in trs120 disrupt traffic from the early endosome to the late Golgi. THE JOURNAL OF CELL BIOLOGY. 171(5): 823-833, https://www.webofscience.com/wos/woscc/full-record/WOS:000233753700009.

科研活动

   
科研项目
( 1 ) 中组部第十二批****青年人才项目, 负责人, 国家任务, 2016-01--2018-12
( 2 ) 细胞迁移相关膜行为的蛋白质机器, 负责人, 国家任务, 2016-06--2021-06
( 3 ) PH结构域蛋白在迁移细胞中的膜定位机制与功能研究, 负责人, 国家任务, 2018-01--2021-12
( 4 ) 内膜系统的完整性维持, 负责人, 国家任务, 2021-12--2026-11
( 5 ) 细胞巨胞饮调控物质转运的机制研究, 负责人, 中国科学院计划, 2020-01--2024-12
( 6 ) VPS13蛋白调控细胞巨胞饮作用的机制和功能研究, 负责人, 国家任务, 2022-01--2025-12
( 7 ) 细胞器互作调控膜完整性稳态的机制研究, 负责人, 国家任务, 2023-01--2025-12
参与会议
(1)Dissecting the Dynamics of Macropinosome Maturation   第五届蛋白质介导的膜塑形与重装国际学术研讨会   2023-11-09
(2)Transcriptional Regulation of Macropinocytosis   2023 国际盘基网柄菌会议   2023-08-27
(3)Macropinocytosis regulation in response to changing nutrient conditions   第一届中国溶酶体生物学大会   2023-04-20
(4)Macropinocytosis regulation in response to changing nutrient conditions    第七次国际暨第十六次全国膜生物学学术研讨会   2021-11-12
(5)Polarized actin assembly during cell migration and macropinocytosis   第二届中国细胞骨架前沿学术会议   2021-07-28
(6)Proteomic analysis of chemoattractant-induced signaling dynamics reveals new cytoskeleton regulators   2019年中国细胞骨架前沿学术会议   2019-11-16
(7)Analysis of chemoattractant-induced signaling dynamics reveals new polarity regulators   2019 国际盘基网柄菌会议   2019-08-04
(8)Polarity Regulation During Cell Migration and Endocytosis   蛋白质介导的膜塑形与重装 国际学术研讨会   2019-04-11
(9)Proteomic Analysis of Chemoattractant-induced Signaling Dynamics    2018 国际盘基网柄菌会议   2018-08-12
(10)Cell Migration and Macropinocytosis    第13届海峡两岸细胞生物学学术研讨会    2018-07-18
(11)Nucleocytoplasmic Shuttling of a GATA Transcription Factor Functions as a Development Timer   2017国际盘基网柄菌会议   2017-08-20
(12)细胞趋化与信号转导   北京细胞生物学会2016年学术大会   2016-10-30
(13)Signal Transduction in Cell Migration   第五次国际暨第十四次全国膜生物学学术研讨会   2016-08-04

指导学生

已指导学生

郝亚洲  博士研究生  071009-细胞生物学  

张奕玮  博士研究生  071009-细胞生物学  

张成钰  博士研究生  071009-细胞生物学  

王若弃  博士研究生  071009-细胞生物学  

涂慧  博士研究生  071009-细胞生物学  

现指导学生

郭虎  博士研究生  071009-细胞生物学  

晁晓婷  博士研究生  071009-细胞生物学  

袁野  博士研究生  071009-细胞生物学  

缪茜琳  博士研究生  071009-细胞生物学  

王志萌  博士研究生  071009-细胞生物学  

邹松霖  博士研究生  071009-细胞生物学  

陈浩宇  博士研究生  071009-细胞生物学  

杨鹃  硕士研究生  071009-细胞生物学  

冯诺  硕士研究生  071000-生物学  

尹彬彬  硕士研究生  071009-细胞生物学