071010-生物化学与分子生物学
086000-生物与医药

蛋白质工程，酶和生物催化，合成生物学
合成生物学，生物工程

1999-09--2002-07   中国科学院微生物研究所   博士

   

2015-07~现在, 中国科学院青岛生物能源与过程研究所, 副研究员
2008-08~2015-03,Imperial College London, UK, Senior Research Associate
2004-10~2007-12,Imperial College London, UK, Research Associate
2002-08~2004-10,纽英伦生物技术（北京）有限公司, 研发部主任兼生产部经理

2018-04-16-今,EDITORIAL BOARD MEMBER of American Journal of Bioscience and Bioengineering (BIO), EDITORIAL BOARD MEMBER

   

（1） 最佳展板奖, 其他, 2018
（2） Best poster prize, The 18th CLSS-UK Annual Meeting. London UK, 其他, 2012
（3） Best poste prize, The XXIInd FECTS Meeting. Davos, Swiss, 其他, 2010
（4） Conference Attendance Award, ICGEB (International Centre for Genetic Engineering and Biotechnology), 其他, 2007

[1] 吕雪峰, 周冠, 徐卉芳, 罗泉, 赵航, 冯岩, 徐扬军, 姚庆佳. 一种生物酶法催化合成(R)-2-(1-氨乙基)-4-氟苯酚的方法. 202210663645.8, 2022-06-14.
[2] 吕雪峰, 徐卉芳, 梁雅静. 一种高效蔗糖磷酸化酶在制备甘油葡糖苷中的应用. 202210479826.5, 2022-04-29.
[3] 吕雪峰, 徐卉芳, 梁雅静. 一种高效蔗糖磷酸化酶及其在生产甘油葡糖苷中的应用. 202210479829.9, 2022-04-29.
[4] 吕雪峰, 梁雅静, 徐卉芳, 刘祥, 吴怀之, 刘维和. 一种四步法分离纯化甘油葡糖苷的方法. 202210479025.9, 2022-04-29.
[5] 吕雪峰, 徐卉芳, 梁雅静. 一种高效生产甘油葡糖苷的蔗糖磷酸化酶及其应用. 202210479024.4, 2022-04-29.
[6] 吕雪峰, 梁雅静, 徐卉芳, 刘祥, 吴怀之, 刘维和. 一种二步法分离纯化甘油葡糖苷的方法. 202210479828.4, 2022-04-29.
[7] 吕雪峰, 徐卉芳, 梁雅静. 一种高效蔗糖磷酸化酶及其在生产甘油葡糖苷中的应用. 202210479827.X, 2022-04-29.
[8] 吕雪峰, 徐卉芳, 梁雅静. 一种耐热蔗糖磷酸化酶在生产甘油葡糖苷中的应用. 202210479136.X, 2022-04-29.
[9] 吕雪峰, 梁雅静, 徐卉芳. 一种甘油葡糖苷生产方法. 202210479830.1, 2022-04-28.
[10] 吕雪峰, 梁雅静, 徐卉芳, 刘维和. 一种酶法合成甘油葡糖苷反应液的过滤纯化系统. 202220925942.0, 2022-04-20.
[11] L.富拉格, F.劳费尔, H.施特鲁布, 王允, 徐健. α-烯烃的生产. FR: CN108779476A, 2018-11-09.

   

[1] Kong, Fanhui, Chen, Jie, Qin, Xiangquan, Liu, Chuanfei, Jiang, Yiping, Ma, Li, Xu, Huifang, Li, Shengying, Cong, Zhiqi. Evolving a P450BM3 Peroxygenase for the Production of Indigoid Dyes from Indoles. CHEMCATCHEM[J]. 2022, 14(24): [2] Jiang, Yuanyuan, Li, Zhong, Zheng, Shanmin, Xu, Huifang, Zhou, Yongjin J, Gao, Zhengquan, Meng, Chunxiao, Li, Shengying. Establishing an enzyme cascade for one-pot production of alpha-olefins from low-cost triglycerides and oils without exogenous H2O2 addition. BIOTECHNOLOGY FOR BIOFUELS[J]. 2020, 13(1): http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000521251800001.
[3] Xu, Huifang, Liang, Weinan, Ning, Linlin, Jiang, Yuanyuan, Yang, Wenxia, Wang, Cong, Qi, Feifei, Ma, Li, Du, Lei, Fourage, Laurent, Zhou, Yongjin J, Li, Shengying. Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity. CHEMCATCHEM[J]. 2020, 12(1): 80-84, https://www.webofscience.com/wos/woscc/full-record/WOS:000492792500001.
[4] Yuanyuan Jiang, Zhong Li, Shanmin Zheng, Huifang Xu, Yongjin J. Zhou, Zhengquan Gao, Chunxiao Meng, Shengying Li. Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H2O2 addition. BIOTECHNOLOGY FOR BIOFUELS[J]. 2020, 13(1): 1-11, http://gooa.las.ac.cn/external/index?type=-1&pid=1578635.
[5] Li, Xiaobo, Xu, Huifang, Mo, Xiaodong, Yang, Huanjie. Sequence-based typing identification of a novel allele HLA-A*30:115. HLAnull. 2019, 94(3): 314-315, http://dx.doi.org/10.1111/tan.13602.
[6] Xing, Xiaoyi, Xu, Huifang, Zhang, Wenzhao, Hou, Haijun, Qin, Hongling, Liu, Yi, Zhang, Limei, Fang, Yunting, Wei, Wenxue, Sheng, Rong. The characteristics of the community structure of typical nitrous oxide-reducing denitrifiers in agricultural soils derived from different parent materials. APPLIED SOIL ECOLOGY[J]. 2019, 142: 8-17, http://dx.doi.org/10.1016/j.apsoil.2019.05.013.
[7] Li, Si, Zhao, Jianbo, Shi, Guirong, Tan, Yuanpeng, Xu, Huifang, Chen, Guang, Lan, Haibo, Lin, Zhiqing. Chinese Grammatical Error Correction Based on Convolutional Sequence to Sequence Model. IEEE ACCESS[J]. 2019, 7: 72905-72913, https://doaj.org/article/fb098062980849e5a89824b3694cfe1f.
[8] , , Cong Wang, Yongjin J Zhou, Huifang Xu, . Biochemical characterization of three new α-olefin-producing P450 fatty acid decarboxylases with a halophilic property. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 12(1): 1-14, https://doaj.org/article/15472cd151d54e7cadec684c800c0a77.
[9] Jiang, Yuanyuan, Li, Zhong, Wang, Cong, Zhou, Yongjin J, Xu, Huifang, Li, Shengying. Biochemical characterization of three new alpha-olefin-producing P450 fatty acid decarboxylases with a halophilic property. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 12: http://cas-ir.dicp.ac.cn/handle/321008/165618.
[10] Li Zhong, Du Lei, Zhang Wei, Zhang Xingwang, Jiang Yuanyuan, Liu Kun, Men Ping, Xu Huifang, Fortman Jeffrey L, Sherman David H, Yu Bing, Gao Song, Li Shengying. Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii .. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 2017, 7095-7104, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJPD2B426AED36A0CC9ED99FDB978DC51CFA&DbName=SJPD_05&DbCode=SJPD&yx=&pr=&URLID=&bsm=.
[11] Fang, Bo, Xu, Huifang, Liu, Yi, Qi, Fengxia, Zhang, Wei, Chen, Hui, Wang, Cong, Wang, Yilin, Yang, Wenxia, Li, Shengying. Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE). SCIENTIFIC REPORTS[J]. 2017, 7: http://ir.qibebt.ac.cn/handle/337004/9344.
[12] Xu, Huifang, Ning, Linlin, Yang, Wenxia, Fang, Bo, Wang, Cong, Wang, Yun, Xu, Jian, Collin, Severine, Laeuffer, Frederic, Fourage, Laurent, Li, Shengying. In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases. BIOTECHNOLOGY FOR BIOFUELS[J]. 2017, 10(1): http://dx.doi.org/10.1186/s13068-017-0894-x.
[13] Li, Zhong, Du, Lei, Zhang, Wei, Zhang, Xingwang, Jiang, Yuanyuan, Liu, Kun, Men, Ping, Xu, Huifang, Fortman, Jeffrey L, Sherman, David H, Yu, Bing, Gao, Song, Li, Shengying. Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2017, 292(17): 7095-7104, http://dx.doi.org/10.1074/jbc.M116.751255.
[14] 孙月, 马莉, 陈辉, 徐卉芳, 郑贤良, 齐凤霞, 李盛英. 脂肪酶-P450脂肪酸脱羧酶融合蛋白催化的a烯烃合成. 科学通报[J]. 2017, 62(31): 3576-3582, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=6145690&detailType=1.
[15] 徐卉芳. Recombinant Collagen Engineered to Bind to Discoidin Domain Receptors Functions as a Receptor Inhibitor. Journal of Biological Chemistry[J]. 2016, [16] An, Bo, Abbonante, Vittorio, Xu, Huifang, Gavriilidou, Despoina, Yoshizumi, Ayumi, Bihan, Dominique, Farndale, Richard W, Kaplan, David L, Balduini, Alessandra, Leitinger, Birgit, Brodsky, Barbara. Recombinant Collagen Engineered to Bind to Discoidin Domain Receptor Functions as a Receptor Inhibitor. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2016, 291(9): 4343-4355, http://dx.doi.org/10.1074/jbc.M115.674507.
[17] 徐卉芳. Molecular and cellular analysis of a novel mutation in DDR2 causing Spondylo-meta-epiphyseal dysplasia with short limbs and abnormal calcifications (SMED-SL). BMC Medical Genetics. 2014, [18] Xu Huifang, Abe Takemoto, Liu Justin K H, Zalivina Irina, Hohenester Erhard, Leitinger Birgit. Normal activation of discoidin domain receptor 1 mutants with disulfide cross-links, insertions, or deletions in the extracellular juxtamembrane region: mechanistic implications.. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 2014, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJPDD0238FFA03FFDC777812A2CD519ECDEB&DbName=SJPD_06&DbCode=SJPD&yx=&pr=&URLID=&bsm=.
[19] Xu, Huifang, Bihan, Dominique, Chang, Francis, Huang, Paul H, Farndale, Richard W, Leitinger, Birgit. Discoidin Domain Receptors Promote alpha 1 beta 1-and alpha 2 beta 1-Integrin Mediated Cell Adhesion to Collagen by Enhancing Integrin Activation. PLOS ONE[J]. 2012, 7(12): https://www.webofscience.com/wos/woscc/full-record/WOS:000312794500114.
[20] Xu, Huifang, Raynal, Nicolas, Stathopoulos, Stavros, Myllyharju, Johanna, Farndale, Richard W, Leitinger, Birgit. Collagen binding specificity of the discoidin domain receptors: Binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. MATRIX BIOLOGY[J]. 2011, 30(1): 16-26, http://dx.doi.org/10.1016/j.matbio.2010.10.004.
[21] Ali, Bassam R, Xu, Huifang, Akawi, Nadia A, John, Anne, Karuvantevida, Noushad S, Langer, Ruth, AlGazali, Lihadh, Leitinger, Birgit. Trafficking defects and loss of ligand binding are the underlying causes of all reported DDR2 missense mutations found in SMED-SL patients. HUMAN MOLECULAR GENETICS[J]. 2010, 19(11): 2239-2250, https://www.webofscience.com/wos/woscc/full-record/WOS:000277448500013.
[22] Xu, HF, Zhang, XE, Zhang, ZP, Zhang, YM, Cass, AEG. Directed evolution of E-coli alkaline phosphatase towards higher catalytic activity. BIOCATALYSIS AND BIOTRANSFORMATION[J]. 2003, 21(1): 41-47, http://www.corc.org.cn/handle/1471x/2376138.
[23] Zhang, XE, Zhou, YH, Zhang, ZP, Xu, HF, Shao, WH, Cass, AEG. Engineering E-coli alkaline phosphatase yields changes of catalytic activity, thermal stability and phosphate inhibition. BIOCATALYSIS AND BIOTRANSFORMATION[J]. 2002, 20(6): 381-389, http://www.corc.org.cn/handle/1471x/2376108.
[24] 徐卉芳, 牛钟相. 牛消化道正常菌群的研究. 山东畜牧兽医[J]. 1999, 39-44, http://lib.cqvip.com/Qikan/Article/Detail?id=3671319.
[25] 徐卉芳, 牛钟相. 肉牛粪便正常菌群研究. 黑龙江畜牧兽医[J]. 1999, 3-4, http://lib.cqvip.com/Qikan/Article/Detail?id=3489618.

   

（ 1 ） Improving C12 Activity of New P450 Decarboxylase Encoding Sequences, 主持, 院级, 2015-07--2017-02
（ 2 ） 基于P450催化元件的高值天然产物生物合成途径重构, 参与, 省级, 2017-08--2019-07
（ 3 ） Proof of Concept of Pathway Integration for α-Olefin Biosynthesis in Photosynthetic Microorganisms, 主持, 院级, 2017-07--2019-07
（ 4 ） P450 脂肪酸脱羧酶的定向进化及催化机理研究, 主持, 国家级, 2018-01--2021-12
（ 5 ） 耦合非天然辅酶的P450脂肪酸衍生细胞工厂, 主持, 市地级, 2018-03--2020-02
（ 6 ） 类囊体膜介导的光驱α-烯烃生物合成研究, 主持, 市地级, 2020-01--2021-12
（ 7 ） 中科斯芬克司药物合成特种酶联合实验室项目, 主持, 院级, 2021-01--2026-01

（1）Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity   2019-10-20
（2）催化脂肪酸脱羧生成末端烯烃的新型P450过氧化物酶的鉴定和酶学表征   2018年中国微生物学会学术年会   2018-10-19
（3）Characterization of novel P450 fatty acids decarboxylases for 1-alkene production   第三届合成微生物学与生物制造暨第八届生物催化与生物合成工程国际学术研讨会   2018-10-12
（4）Characterization of novel P450 peroxygenases for oxidative decarboxylation of free fatty acids to terminal alkenes   2018-06-30
（5）学术交流   全国首届生物传感、生物芯片高端论坛   2017-10-20
（6）学术交流   国际化学生物学年会   2017-10-17
（7）学术交流   2016-10-16
（8）Understanding the molecular mechanism for discoidin domain receptor activation   2013-09-01
（9）Discoidin Domain Receptors Promote β1-integrin Mediated Cell Adhesion to Collagen   2012-09-07
（10）The role of discoidin domain receptors in cell adhesion   2012-04-02
（11）The role of DDR Receptors in cell adhesion   2011-09-05
（12）Trafficking defects and loss of ligand binding are the underlying causes of all reported DDR2 missense mutations found in SMED-SL patients   2010-07-03
（13）Determining the collagen binding specificity of the discoidin domain receptors (DDRs)   2010-03-29
（14）Characterization of polyomavirus middle T antigen complexes using affinity purification and MS   2007-07-22

已指导学生

宁琳琳  硕士研究生  085238-生物工程  

马娜娜  硕士研究生  085238-生物工程  

梁伟男  硕士研究生  071010-生物化学与分子生物学