病毒载体技术、工艺开发及评价

   

071005-微生物学
071006-神经生物学

神经环路病毒跨突触示踪技术及工具
溶瘤病毒、基因治疗载体开发及应用

2008-09--2012-06   中国科学院武汉病毒研究所   博士
2004-09--2007-06   湖南师范大学   硕士

   

2019-11~现在, 中国科学院深圳先进技术研究院, 副研究员
2016-07~2019-10,中国科学院武汉物理与数学研究所, 副研究员
2013-09~2016-04,中国科学院武汉物理与数学研究所, 博士后

   

[1] 王华东, 徐富强, 刘奇, 应敏, 冯子健. 抗人类白细胞抗原-G纳米抗体及其制备方法与应用. CN202211526420.4, 2022-12-01.

[2] 王华东, 徐富强, 应敏, 刘同钽, 史青. 靶向HSV必需基因的gRNA、CRISPR/Cas基因编辑系统、递送系统及应用. CN202211526420.4, 2022-12-01.

[3] 王华东, 徐富强, 刘奇, 应敏, 冯子健. 抗人类白细胞抗原-G纳米抗体及其制备方法与应用. PCT/CN2022/134420, 2022-11-25.

[4] 王华东, 徐富强, 应敏, 史青, 卢国刚. 可视化肿瘤细胞系及其构建和应用、肿瘤-神经系统互作神经网络的解析方法. PCT/CN2022/134486, 2022-11-25.

[5] 王华东, 徐富强, 应敏, 林坤章, 史青, 卢国刚. 可视化肿瘤细胞系及其构建和应用、肿瘤-神经系统互作神经网络的解析方法. CN202211490902.9, 2022-11-25.

[6] 王华东, 徐富强, 应敏. 一种中国HSV 临床分离株及基于其的溶瘤病毒和应用. CN202210660127.0, 2022-06-13.

[7] 刘海霞, 刘昕, 王华东, 孙兴怀, 徐富强, 马琳, 应敏. 青光眼睫状体炎综合征动物模型的构建方法及该动物模型. CN202210041673.6, 2022-01-14.

[8] 王华东, 徐富强, 应敏, 刘昕. 一种减毒、复制可控的顺向HSV示踪系统及其构建方法和应用. CN202111524089.8, 2021-12-14.

[9] 王华东, 刘奇, 徐富强, 苏鹏. 基于LAMP联合Cas13a核酸酶的检测痕量核酸的方法以及应用. CN202111423778.X, 2021-11-26.

[10] 王华东, 徐富强, 应敏, 苏鹏, 刘奇. 基于中国HSV临床分离株的溶瘤病毒及其构建方法和应用. CN202111357340.6, 2021-11-16.

[11] 刘奇, 苏鹏, 王华东. 重组单纯疱疹病毒的亲和筛选细胞系及其构建方法和应用. CN202111233995.2, 2021-10-22.

[12] 苏鹏, 王华东, 徐富强. 一种神经环路标记系统. CN: CN112501137A, 2021-03-16.

[13] 王华东, 徐富强, 苏鹏, 夏金金, 胡亮, 李颖利, 钟鑫. 低毒单纯疱疹病毒系统及其构建方法和应用. CN: CN110117577A, 2019-08-13.

[14] 王华东, 徐富强, 夏金金, 苏鹏, 李颖利, 林坤章. 一种携带荧光Timer基因可变色的重组单纯疱疹病毒及制备方法和应用. CN: CN110029127A, 2019-07-19.

[15] 王华东, 徐富强, 何晓斌, 靳森, 郑宁. 一种减毒增亮、复制可控的HSV重组病毒及制备方法和应用. CN: CN107630009A, 2018-01-26.

   

[1] Jgamadze, Dennis, Lim, James T, Zhang, Zhijian, Harary, Paul M, Germi, James, MensahBrown, Kobina, Adam, Christopher D, Mirzakhalili, Ehsan, Singh, Shikha, Gu, Jiahe Ben, Blue, Rachel, Dedhia, Mehek, Fu, Marissa, Jacob, Fadi, Qian, Xuyu, Gagnon, Kimberly, Sergison, Matthew, Fruchet, Oceane, Rahaman, Imon, Wang, Huadong, Xu, Fuqiang, Xiao, Rui, Contreras, Diego, Wolf, John A, Song, Hongjun, Ming, Guoli, Chen, HanChiao Isaac. Structural and functional integration of human forebrain organoids with the injured adult rat visual system. CELL STEM CELL[J]. 2023, 30(2): 137-+, http://dx.doi.org/10.1016/j.stem.2023.01.004.
[2] Min Ying, Huadong Wang, Tongtan Liu, Zengpeng Han, Kunzhang Lin, Qing Shi, Ning Zheng, Tao Ye, Huinan Gong, Fuqiang Xu, Malgorzata Krzyzowska. CLEAR Strategy Inhibited HSV Proliferation Using Viral Vectors Delivered CRISPR-Cas9. PATHOGENS[J]. 2023, 12(6): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302303/.
[3] Bing Cai, Dan Wu, Hong Xie, Yan Chen, Huadong Wang, Sen Jin, Yuran Song, Anan Li, Shiqi Huang, Sashuang Wang, Yingjin Lu, Lan Bao, Fuqiang Xu, Hui Gong, Changlin Li, Xu Zhang. A direct spino-cortical circuit bypassing the thalamus modulates nociception. Cell Research[J]. 2023, https://www.nature.com/articles/s41422-023-00832-0.
[4] Ma, Lin, Yang, Fang, Liu, Qing, Zhu, XuTao, Liu, Xin, Jin, Sen, Wang, Huadong, Pei, Lei, Xu, Fuqiang, Liu, Haixia. Anatomical Evidence for the Neural Connection from the Emotional Brain to Autonomic Innervation in the Anterior Chamber Structures of the Eye. CURRENT MEDICAL SCIENCE[J]. 2022, 42(2): 417-425, http://dx.doi.org/10.1007/s11596-022-2571-y.
[5] Xin Liu, Huadong Wang, Lin Ma, Min Ying, Qing Liu, Heng Chang, Sen Jin, Fang Yang, Xutao Zhu, Xinghuai Sun, Fuqiang Xu, Haixia Liu. Construction of a mouse model of Posner-Schlossman syndrome by anterior chamber infection with cytomegalovirus. EXPERIMENTALEYERESEARCH[J]. 2022, 218: [6] Yan Chen, Ranyu Song, Huadong Wang, Yunyi Zhang, Xinyu Hu, Xu Zhang. Distinct neural networks derived from galanin-containing nociceptors and neurotensin-expressing pruriceptors. Proc Natl Acad Sci U S A[J]. 2022, 119(33): 1-12, https://pubmed.ncbi.nlm.nih.gov/35943985/.
[7] Yang, Fang, Zhu, Xutao, Liu, Xin, Ma, Lin, Zhang, Zhijian, Pei, Lei, Wang, Huadong, Xu, Fuqiang, Liu, Haixia. Anatomical evidence for the efferent pathway from the hypothalamus to autonomic innervation in the anterior chamber structures of eyes. EXPERIMENTAL EYE RESEARCH[J]. 2021, 202: http://dx.doi.org/10.1016/j.exer.2020.108367.
[8] YuFeng Shao, Can Wang, XiaoPing Rao, HuaDong Wang, YanLi Ren, Jing Li, ChaoYu Dong, JunFan Xie, XingWen Yang, FuQiang Xu, YiPing Hou. Neuropeptide S Attenuates the Alarm Pheromone-Evoked Defensive and Risk Assessment Behaviors Through Activation of Cognate Receptor-Expressing Neurons in the Posterior Medial Amygdala. FRONTIERS IN MOLECULAR NEUROSCIENCE[J]. 2021, 14: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739225/.
[9] 钟鑫, 苏鹏, 夏金金, 应敏, 王华东, 徐富强. HSV顺行示踪病毒轴突末梢感染及后续跨突触传播. 兰州大学学报(自然科学版)[J]. 2020, 56(06期): 799-806, [10] Peng Su, Min Ying, Zengpeng Han, Jinjin Xia, Sen Jin, Yingli Li, Huadong Wang, Fuqiang Xu. High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy. MOLECULAR BRAIN[J]. 2020, 13(1): 1-13, http://dx.doi.org/10.1186/s13041-020-0544-2.
[11] Yao, Yanfeng, Wang, Huadong, Chen, Jianjun, Shao, Zhiyong, He, Bin, Chen, Jie, Lan, Jiaming, Chen, Quanjiao, Chen, Ze. Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine. EMERGING MICROBES & INFECTIONS[J]. 2019, 8(1): 45-54, http://202.127.146.157/handle/2RYDP1HH/6512.
[12] Zheng, Ning, Su, Peng, Liu, Yue, Wang, Huadong, Nie, Binbin, Fang, Xiaohui, Xu, Yue, Lin, Kunzhang, Lv, Pei, He, Xiaobin, Guo, Yi, Shan, Baoci, Manyande, Anne, Wang, Jie, Xu, Fuqiang. Detection of neural connections with ex vivo MRI using a ferritin-encoding trans-synaptic virus. NEUROIMAGE[J]. 2019, 197: 133-142, http://dx.doi.org/10.1016/j.neuroimage.2019.04.039.
[13] 韩增鹏, 施祥玮, 应敏, 李梅, 郑宁, 张志建, 吴阳, 王华东, 王杰, 贾凡, 徐富强. 神经环路示踪工具病毒的研究进展. 分析化学[J]. 2019, [14] Yu, FeiLong, Miao, Huan, Xia, Jinjin, Jia, Fan, Wang, Huadong, Xu, Fuqiang, Guo, Lin. Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell-Cell Transmission. PROTEOMICS[J]. 2019, 19(23): [15] Su, Peng, Wang, Huadong, Xia, Jinjin, Zhong, Xin, Hu, Liang, Li, Yingli, Li, Yanqiu, Ying, Min, Xu, Fuqiang. Evaluation of retrograde labeling profiles of HSV1 H129 anterograde tracer. JOURNAL OF CHEMICAL NEUROANATOMY[J]. 2019, 100: 101662-, [16] Zhang, Zhijian, Zhang, Hongruo, Wen, Pengjie, Zhu, Xutao, Wang, Li, Liu, Qing, Wang, Jie, He, Xiaobin, Wang, Huadong, Xu, Fuqiang. Whole-Brain Mapping of the Inputs and Outputs of the Medial Part of the Olfactory Tubercle. FRONTIERS IN NEURAL CIRCUITS[J]. 2017, 11: https://doaj.org/article/94526d48f15341b48bae7fe3cc1e3490.
[17] Tang, He, Wu, GuiSheng, Xie, Jing, He, Xiaobin, Deng, Ke, Wang, Huadong, Xu, Fuqiang, Luo, HuaiRong. Brain-wide map of projections from mice ventral subiculum. NEUROSCIENCE LETTERS[J]. 2016, 629: 171-179, http://dx.doi.org/10.1016/j.neulet.2016.07.014.
[18] Wang, Huadong, Huang, Chaoyang, Dong, Jinrong, Yao, Yanfeng, Xie, Zhenyuan, Liu, Xueying, Zhang, Wenjie, Fang, Fang, Chen, Ze. Complete Protection of Mice against Lethal Murine Cytomegalovirus Challenge by Immunization with DNA Vaccines Encoding Envelope Glycoprotein Complex III Antigens gH, gL and gO. PLOS ONE[J]. 2015, 10(3): [19] Chen, Jianjun, Liu, Qian, Chen, Quanjiao, Xiong, Chaochao, Yao, Yanfeng, Wang, Huadong, Wang, Hanzhong, Chen, Ze. Comparative analysis of antibody induction and protection against influenza virus infection by DNA immunization with HA, HAe, and HA1 in mice. ARCHIVES OF VIROLOGY[J]. 2014, 159(4): 689-700, https://www.webofscience.com/wos/woscc/full-record/WOS:000334055200008.
[20] Chen, Jianjun, Yan, Bin, Chen, Quanjiao, Yao, Yanfeng, Wang, Huadong, Liu, Qian, Zhang, Shaoqiong, Wang, Hanzhong, Chen, Ze. Evaluation of neutralizing efficacy of monoclonal antibodies specific for 2009 pandemic H1N1 influenza A virus in vitro and in vivo. ARCHIVES OF VIROLOGY[J]. 2014, 159(3): 471-483, [21] Huang, Chaoyang, Wang, Huadong, Wu, Shuting, Chang, Haiyan, Liu, Lalan, Peng, Bo, Fang, Fang, Chen, Ze. Comparison of multiple DNA vaccines for protection against cytomegalovirus infection in BALB/c mice. VIROLOGY JOURNAL[J]. 2014, 11(1): [22] Wang, Huadong, Yao, Yanfeng, Huang, Chaoyang, Fu, Xingxing, Chen, Quanjiao, Zhang, Hongbo, Chen, Jianjun, Fang, Fang, Xie, Zhenyuan, Chen, Ze. An adjuvanted inactivated murine cytomegalovirus (MCMV) vaccine induces potent and long-term protective immunity against a lethal challenge with virulent MCMV. BMC INFECTIOUS DISEASES[J]. 2014, 14(1): http://dx.doi.org/10.1186/1471-2334-14-195.
[23] 张志建, 靳森, 朱续涛, 贾凡, 王华东, 刘青, 何晓斌, 徐富强. 利用嗜神经病毒跨突触追踪神经网络研究进展. 生命科学[J]. 2014, 26(6): 634-, [24] Wang Huadong, Yao Yanfeng, Huang Chaoyang, Chen Quanjiao, Chen Jianjun, Chen Ze. Immunization with Cytomegalovirus Envelope Glycoprotein M and Glycoprotein N DNA Vaccines can Provide Mice with Complete Protection against a Lethal Murine Cytomegalovirus Challenge. 中国病毒学：英文版[J]. 2013, 174-182, http://lib.cqvip.com/Qikan/Article/Detail?id=46210376.
[25] Chaochao Xiong, Qian Liu, Quanjiao Chen, Yanfeng Yao, Huadong Wang, Jianjun Chen. Complete Genome Sequence of a Novel Reassortant H3N6 Avian Influenza Virus Isolated from Domestic Green-Winged Teal. GENOME ANNOUNCEMENTS. 2013, 1(3): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656212/.
[26] Huang, Shengping, Chen, Jingjing, Chen, Quanjiao, Wang, Huadong, Yao, Yanfeng, Chen, Jianjun, Chen, Ze. A Second CRM1-Dependent Nuclear Export Signal in the Influenza A Virus NS2 Protein Contributes to the Nuclear Export of Viral Ribonucleoproteins. JOURNAL OF VIROLOGY[J]. 2013, 87(2): 767-778, [27] Yao, Yanfeng, Wang, Huadong, Chen, Quanjiao, Zhang, Hongbo, Zhang, Tao, Chen, Jianjun, Xu, Bing, Wang, Hanzhong, Sun, Bing, Chen, Ze. Characterization of low-pathogenic H6N6 avian influenza viruses in central China. ARCHIVES OF VIROLOGY[J]. 2013, 158(2): 367-377, [28] Huang, Shengping, Chen, Jingjing, Wang, Huadong, Sun, Bing, Wang, Hanzhong, Zhang, Zhiping, Zhang, Xianen, Chen, Ze. Influenza A virus matrix protein 1 interacts with hTFIIIC102-s, a short isoform of the polypeptide 3 subunit of human general transcription factor IIIC. ARCHIVES OF VIROLOGY[J]. 2009, 154(7): 1101-1110, http://www.corc.org.cn/handle/1471x/2375555.
[29] Chen, Quanjiao, Kuang, Haimen, Wang, Huadong, Fang, Fang, Yang, Zhongdong, Zhang, Zhiping, Zhang, Xianen, Chen, Ze. Comparing the ability of a series of viral protein-expressing plasmid DNAs to protect against H5N1 influenza virus. VIRUS GENES[J]. 2009, 38(1): 30-38, http://www.corc.org.cn/handle/1471x/2375552.
[30] Zhou, Yu, Fang, Fang, Chen, Jianjun, Wang, Huadong, Chang, Haiyan, Yang, Zhongdong, Chen, Ze. Electroporation at Low Voltages Enables DNA Vaccine to Provide Protection against a Lethal H5N1 Avian Influenza Virus Challenge in Mice. INTERVIROLOGY[J]. 2008, 51(4): 241-246, http://www.corc.org.cn/handle/1471x/2375500.

   

（ 1 ） 构建细胞选择、复制可控、顺行跨突触、结构与功能研究兼顾的单纯疱疹工具病毒体系, 主持, 国家级, 2015-01--2017-12
（ 2 ） 基于HSV的神经环路示踪系统, 主持, 市地级, 2017-01--2018-12
（ 3 ） 建立低毒、高亮、细胞特异、环路结构与功能兼顾的HSV示踪体系, 主持, 国家级, 2018-01--2021-12
（ 4 ） 建立灵敏、减毒、级数可控的顺行跨突触VSV病毒示踪系统, 参与, 国家级, 2017-01--2020-12
（ 5 ） “窝/安全空间”记忆形成及其调控恐惧反应的环路机制, 参与, 国家级, 2017-01--2019-12
（ 6 ） 灵长类动物脑细胞和神经回路的特异性光学标记, 参与, 国家级, 2015-01--2019-08
（ 7 ） 神经回路示踪及其在感知觉等联结图谱研究中的应用, 参与, 部委级, 2012-07--2017-06