纳米孔单分子检测技术及其应用

硕士研究生（具有生物化学/物理，分子生物学，分析化学相关专业背景优先）

071010-生物化学与分子生物学
080300-光学工程
070302-分析化学

纳米孔单分子检测
生物自组装材料

2008-09--2011-06   四川大学   博士研究生

研究生

理学博士

   

2018-01~现在, 中科院重庆绿色智能技术研究院, 研究员
2017-02~2017-07,美国俄亥俄州立大学药学院, 博士后研究员
2012-10~2018-06,美国伊利诺伊理工大学, 高级助理研究员
2011-07~2012-09,江苏大学, 助理研究员

2018-04-10-今,河北省科技厅科技奖励评审专家, 评审专家

重庆英才·青年拔尖人才（首批）

（1） 重庆市北碚区第7届”十大杰出青年“, 市地级, 2022
（2） 重庆市青年科技创新先锋人物, 省级, 2022
（3） 重庆英才·青年拔尖人才, , 省级, 2019

（ 1 ） 纳米孔在电化学检测植物抗性基因表达多肽中的应用及检测方法, 发明专利, 2021, 第 1 作者, 专利号: CN113804869A

（ 2 ） 一种基于氮化硅（SiNx）固态纳米孔检测HIV-1 P24抗原的方法及装置, 发明专利, 2021, 第 5 作者, 专利号: CN113219015A

（ 3 ） 基于固态纳米孔的HIV-1抗体检测装置及方法, 发明专利, 2021, 第 5 作者, 专利号: CN113176322A

（ 4 ） 基于固态纳米孔的HIV-1抗体+抗原检测装置及方法, 发明专利, 2021, 第 5 作者, 专利号: CN113176323A

（ 5 ） 一种全血HIV检测装置, 发明专利, 2021, 第 4 作者, 专利号: CN112782399A

（ 6 ） 金属离子在影响诱导HIV-1的Tat蛋白水解中的应用及其检测方法, 发明专利, 2021, 第 1 作者, 专利号: CN112505126A

（ 7 ） 一种基于纳米孔的体外HIV蛋白酶检测仪器, 发明专利, 2021, 第 4 作者, 专利号: CN112345771A

（ 8 ） 一种纳米晶石墨纳米孔检测芯片及其制备方法和应用, 发明专利, 2020, 第 3 作者, 专利号: CN112014430A

（ 9 ） 基于纳米孔的体外HIV蛋白酶检测仪器, 实用新型, 2020, 第 5 作者, 专利号: CN211374784U

（ 10 ） 一种基于纳米通道的抗体单分子检测系统, 发明专利, 2020, 第 1 作者, 专利号: CN210953911U

（ 11 ） 一种基于纳米孔水解反应的免疫球蛋白M检测方法, 发明专利, 2020, 第 3 作者, 专利号: CN111323469A

（ 12 ） 一种基于纳米通道的抗体单分子检测系统及方法, 发明专利, 2019, 第 1 作者, 专利号: CN110554079A

（ 13 ） 一种自动精确定位制备固态纳米孔阵列的系统及方法, 发明专利, 2019, 第 11 作者, 专利号: CN110436406A

（ 14 ） 基于纳米孔的体外HIV蛋白酶检测仪器及方法, 发明专利, 2019, 第 4 作者, 专利号: CN110346579A

近5年发表论文

1.       Xiaohan Chen, Shuo Zhou, Yunjiao Wang, Ling Zheng, Sarah Guan, Deqiang Wang, Liang Wang* and Xiyun Guan*. Nanopore single-molecule analysis of biomarkers: Providing possible clues to disease diagnosis. Trends in Analytical Chemistry. 2023. 162. 117060.

2.       Shaoxia Zhang, Yunjiao Wang, Dandan Song, Sarah Guan, Daming Zhou, Linyu Gong, Liyuan Liang, Xiyun Guan* and Liang Wang*. Nanopore discrimination and sensitive plasma detection of multiple natriuretic peptides: The representative biomarker of human heart failure. Biosensors Bioelectronics. 2023. 231. 115299.

3.       Dandan Song, Yunjiao Wang, Shaoxia Zhang, Lingyu Zhao, Linyu Gong, Liyuan Liang and Liang Wang*. Single-molecule Measurement of Carbonic Anhydrase in Cation Coordinated Environment Using MspA Nanopore. Chinese Journal of Chemistry. 2023. 41. 1341-1347.

4.       Minghan Li, Shanchuan Chen, Yunjiao Wang, Shaoxia Zhang, Dandan Song, Rong Tian, Jia Geng, Liang Wang.* Label-free high-precise nanopore detection of endopeptidase activity of anthrax lethal factor regulated by diverse conditions. Biosensors Bioelectronics. 2023. 219. 114800.

5.       Wei Li, Yunjiao Wang, Yicen Xiao, Minghan Li, Qianshan Liu, Liyuan Liang, Wanyi Xie, Deqiang Wang*, Xiyun Guan*, and Liang Wang*. Simultaneous Dual-Site Identification of 5^mC/8^oG in DNA Triplex Using a Nanopore Sensor. ACS Applied Materials Interfaces. 2022. 14. 32948.

6.       Rong Tian, Bohua Yin, Dejin Liu, Qianshan Liu, Shanchuan Chen, Minghan Li, Liang Wang, Shuo Zhou, Deqiang Wang. Highly sensitive α-Hemolysin nanopore detection of MUC1 based on 3D DNA walker. Analytica Chimica Acta. 2022. 1223.340193.

7.       Liang Wang, Han Wang, Xiaohan Chen, Shuo Zhou, Yunjiao Wang, * and Xiyun Guan*. Chemistry solutions to facilitate nanopore detection and analysis. Biosensors Bioelectronics. 2022. 213. 114448.

8.       Yicen Xiao, Juansheng Ren, Yunjiao Wang, Xiaohan Chen, Shuo Zhou, Minghan Li, Fangyuan Gao, Liyuan Liang, Deqiang Wang, Guangjun Ren* and Liang Wang *. De novo Profiling of Insect-resistant Proteins of Rice via Nanopore Peptide Differentiation. Biosensors Bioelectronics. 2022. 212. 114415.

9.       Ji Wu, Liyuan Liang*, Mingkun Zhang, Rui Zhu, Zhong Wang, Yajie Yin, Bohua Yin, Ting Weng, Shaoxi Fang, Wanyi Xie, Liang Wang, and Deqiang Wang. Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores. ACS Appl. Mater. Interfaces 2022, 14, 10, 12077.

10.    Yunjiao Wang, Zequn Wang, Liang Wang, Tianze Tong, Xiaoling Zhang, Shaoxi Fang, Wanyi Xie, Liyuan Liang, Bohua Yin, Jiahu Yuan*, Jin Zhang*, and Deqiang Wang*. Comparison Study on Single Nucleotide Transport Phenomena in Carbon Nanotubes. Nano Letters 2022, 22, 5, 2147.

11.    Han Wang, Wenli Huang, Yunjiao Wang, Wei Li, qianshan liu, Bohua Yin, Liyuan Liang, Deqiang Wang, Xiyun Guan*, Liang Wang*. Enzyme hinders HIV-1 Tat viral transport and real-time measured with nanopores. ACS Sensors. 2021. 6. 3781.

12.    Xiaoqing Zeng, Yang Xiang, Qianshan Liu, Liang Wang, Qianyun Ma, Wenhao Ma, Delin Zeng, Yajie Yin, Deqiang Wang. Nanopore Technology for the Application of Protein Detection. Nanomaterials. 2021. 11. 1942.

13.    Ji Wu, Sen Wang, Liyuan Liang, Chuanqi Zhao, Yajie Yin, Ting Weng, Bohua Yin, Liang Wang, Deqiang Wang. Investigation on the competition of duplex/ G-quadruplex/ i-motif in telomere sequences and c-MYC gene with a solid-state nanopore sensor. Sensors and Actuators B: Chemical. 2021. 348.13072.

14.    Minghan Li, Wei Li, Yicen Xiao, Qianshan Liu, Liyuan Liang, Deqiang Wang, Wenli Huang^* and Liang Wang^*. Nanopore Detects γ-Radiation Inhibited HIV-1 Protease Activity. Biosensors Bioelectronics. 2021. 194. 113602.

15.    Yunjiao Wang, Min Cheng, Liang Wang, Daming Zhou, Shixuan He, Liyuan Liang, Feng Zhang, Chang Liu, Deqiang Wang and Jiahu Yuan. Nanocrystalline Graphite Nanopores for DNA Sensing. Carbon. 2021. 176. 271.

16.    Wanyi Xie, Shixuan He, Shaoxi Fang, Daming Zhou, Yongna Zhang, Liyuan Liang, Liang Wang, and Deqiang Wang. The Raman band shift of suspended graphene impacted by the substrate edge and helium ion irradiation. Nano express. 2021.2.010001.

17.    Qianshan Liu, Yunjiao Wang, Yaqing Liu, Han Wang, Wei Li, Peng Tang, Ting Weng, Shuo Zhou, Liyuan Liang, Jiahu Yuan*, Deqiang Wang*, and Liang Wang*. Reduction Chemistry-Assisted Nanopore Determination Method for Immunoglobulins Isotypes. Nanoscale. 2020. 12. 19711.

18.    Jing Tang, Sen Wang, Ji Wu, Liyuan Liang, Liang Wang, Deqiang Wang. Applications of Photo-Responsive Molecules in Nanopore-based Devices. Chinese Journal of Analytical Chemistry. 2020. 48. 1458.

19.    Yunjiao Wang, Youwen Zhang, Xiaohan Chen, Xiyun Guan*, Liang Wang*. Analytical Strategies with Biological Nanopore: On-pore, off-pore Strategies and application in Biological Fluids. Talanta. 2021. 223. 121684.

20.    Youwen Zhang, Xiaohan Chen, Shaoqing Yuan, Liang Wang*, and Xiyun Guan*. Joint Entropy-assisted Graphene Oxide-based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases. Analytical Chemistry. 2020. 22. 15042.

21.    Yao Cai, Baocai Zhang, Liyuan Liang, Sen Wang, Lanjun Zhang, Liang Wang, Hongliang Cui, Yihua Zhou, Deqiang Wang. A Solid-State Nanopore-Based Single-Molecule Approach for Label-Free Characterization of Plant Polysaccharides. Plant Communication. 2020. 1. 100106.

22.    Golbarg Mohammadi Roozbahani, Xiaohan Chen, Youwen Zhang, Liang Wang* and Xiyun Guan*. Nanopore Detection of Metal Ions: Current Status and Future Directions. Small Methods. 2020. 4. 2000266.

23.    Sen Wang, Liyuan Liang, Jing Tang, Yao Cai, Chuanqi Zhao, Shaoxi Fang, Huabin Wang, Ting Weng, Liang Wang and Deqiang Wang. Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor. RSC Advances. 2020,10, 27215-27224.

24.    Bohua Yin, Shaoxi Fang, Daming Zhou, Liyuan Liang, Liang Wang, and Zuobin Wang, Deqiang Wang, Jiahu Yuan. Nanopore Fabrication via Transient High Electric Field Controlled Breakdown and Detection of Single RNA Molecules. ACS Applied Bio Materials. 2020. 3. 6368.

25.    Shuo Zhou, Han Wang, Xiaohan Chen, Yunjiao Wang, Daming Zhou, Liyuan Liang, Liang Wang,* Deqiang Wang,* and Xiyun Guan*. Single-Molecule Study on Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore. ACS Appl. Bio Mater. 2020, 3, 554-560.

26.    Liang Wang, Xiaohan Chen, Yunjiao Wang, Shuo Zhou, Deqiang Wang, Xiyun Guan. Nanopore Determination of Nucleic Acids in Whole Blood Based on a Displacement Reaction Strategy. Biophysical J. 2020.118.3.475A.

27.    Xiaohan Chen#, Liang Wang#, Golbarg M Roozbahani, Youwen Zhang, Jialing Xiang, Xiyun Guan，Nanopore label-free detection of single-nucleotide deletion in Baxa/BaxΔ2. Electrophoresis, 2018，39，2410-2416. (co-first author)

28.    Shuo Zhou, Peng Tang, Yunjiao Wang, Liang Wang^※, Deqiang Wang^※, Application of nanopore sensing in detection of toxic molecules. Chinese Journal Analytical Chemistry, 2018,46(6), 826-835.

29.    Liang Wang, Shuo Zhou, Xiaohan Chen, Xiyun Guan, Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices. Chemical Communication. 2018, 54，13977-13980.

   

（ 1 ） 基于纳米孔技术的生物分子单分子检测研究, 负责人, 中国科学院计划, 2018-04--2020-04
（ 2 ） 水稻抗褐飞虱基因编码SCR蛋白在跨膜通道中迁移的研究, 负责人, 国家任务, 2019-01--2021-12
（ 3 ） 水稻抗褐飞虱基因编码SCR蛋白的纳米孔研究, 负责人, 地方任务, 2018-08--2021-07
（ 4 ） 重庆英才·青年拔尖人才项目, 负责人, 地方任务, 2020-01--2022-12
（ 5 ） 活细胞内生物量动态检测纳米孔传感器件, 参与, 国家任务, 2022-12--2025-11

（1）基于纳米孔单分子检测的微囊藻毒素-LR研究   中国化学会第13届全国分析化学年会   2018-06-14
（2）Label-free real-time measurement of protease activity by    美国化学学会太平洋化学会议   X. Guan, L. Wang, S. Zhou, X. Chen   2015-12-15
（3）Nanopore Stochastic Sensing: A Versatile Tool   美国化学会芝加哥分会   L. Wang, S. Zhou, X. Chen, X. Guan   2015-04-23
（4）Nanopore Back Titration Analysis of Dipicolinic Acid   249届美国化学会会议   2015-03-22
（5）Real-time Label-free Nanopore Sensor for the Detection    249届美国化学学会会议   2015-03-22
（6）Real-time Label-free Measurement of HIV-1 Protease    248届美国化学学会会议   2014-08-10
（7）A Label-Free Real-Time cDNA Sensor for Infectious    匹兹堡会议   2014-03-02

已指导学生

王涵  硕士研究生  085202-光学工程  

肖钇岑  硕士研究生  085202-光学工程  

李苇  博士研究生  080300-光学工程  

现指导学生

陈云昊  硕士研究生  085400-电子信息  

刘千山  博士研究生  080300-光学工程  

王琰  硕士研究生  085400-电子信息