化学生物学

2005-09--2010-06   中国科学技术大学   博士
2001-09--2005-07   中国科学技术大学   学士

   

2016-01~现在, 中国科学院上海有机化学研究所, 研究员
2010-08~2016-01,美国Scipps研究所, 博士后助理研究员

最新论文情况：

http://ttlab.top/Publications.htm

https://orcid.org/0000-0002-3448-5494

https://www.researchgate.net/profile/Pengfei-Fang​

[1] Lin, Yuqi, Yu, Biao, Fang, Pengfei, Wang, Jing. Inhibiting autophagy before it starts.. Autophagy[J]. 2023, [2] Zeng，Qi-Yu, Zhang， Fan, Zhang，Jian-Hui, Hei， Zhoufei, Li, Zi-Han, Huang, Meng-Han, Fang, Pengfei, Wang, En-Duo, Sun, Xiaojian, Zhou, Xiao-Long. Loss of threonyl-tRNA synthetase-like protein Tarsl2 has little impact on protein synthesis but affects mouse development.. J Biol Chem[J]. 2023, 299(5): 104704-, [3] Dai, Yuhan, Cheng, Yiyuan, Ding, Weizhong, Qiao, Hang, Zhang, Derundong, Zhong, Guannan, Xia, Mingyu, Tao, Jiang, Sun, Peng, Fang, Pengfei, Liu, Wen. Structural Basis of Low-Molecular-Weight Thiol Glycosylation in Lincomycin A Biosynthesis. ACS CHEMICAL BIOLOGY[J]. 2023, 18(6): 1271-1277, http://dx.doi.org/10.1021/acschembio.3c00185.
[4] He, Siyu, Chu, Xianglin, Wu, Yujia, Jiang, Jiheng, Fang, Pengfei, Chen, Yuting, Liu, Yang, Qiu, Zhixia, Xiao, Yibei, Li, Zhiyu, Pan, Di, Zhang, Qian, Xie, Huanfang, Xing, Shuaishuai, Feng, Feng, Liu, Wenyuan, Guo, Qinglong, Zhao, Li, Yang, Peng, Sun, Haopeng. Development of Biaryl-Containing Aldo-Keto Reductase 1C3 (AKR1C3) Inhibitors for Reversing AKR1C3-Mediated Drug Resistance in Cancer Treatment. JOURNAL OF MEDICINAL CHEMISTRY[J]. 2023, 66(14): 9537-9560, http://dx.doi.org/10.1021/acs.jmedchem.3c00213.
[5] Tang, Yingke, Behrens, Ryan T, Gelais, Corine St, Wu, Siqi, Vivekanandan, Saravanan, Ehud, Razin, Fang, Pengfei, Wu, Li, Sherer, Nathan, Musier-Forsyth, Karin. Human lysyl-tRNA synthetase phosphorylation promotes HIV-1 proviral DNA transcription. Nucleic Acids Research[J]. 2023, [6] Huang, Cao, Xia, Mingyu, Qiao, Hang, Liu, Zaizhou, Lin, Yuqi, Sun, Hanyin, Yu, Biao, Fang, Pengfei, Wang, Jing. Tetramerization of upstream stimulating factor USF2 requires the elongated bent leucine zipper of the bHLH-LZ domain. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2023, 299(10): http://dx.doi.org/10.1016/j.jbc.2023.105240.
[7] Qiao, Hang, Xia, Mingyu, Cheng, Yiyuan, Zhou, Jintong, Zheng, Li, Li, Wei, Wang, Jing, Fang, Pengfei. Tyrosine-targeted covalent inhibition of a tRNA synthetase aided by zinc ion. COMMUNICATIONS BIOLOGY[J]. 2023, 6(1): http://dx.doi.org/10.1038/s42003-023-04517-7.
[8] Liu, Zaizhou, Chen, Kaige, Dai, Jun, Xu, Peng, Sun, Wei, Liu, Wanlin, Zhao, Zhixin, Bennett, Steven P, Li, Peifeng, Ma, Tiancheng, Lin, Yuqi, Kawakami, Akinori, Yu, Jing, Wang, Fei, Wang, Chunxi, Li, Miao, Chase, Peter, Hodder, Peter, Spicer, Timothy P, Scampavia, Louis, Cao, Chunyang, Pan, Lifeng, Dong, Jiajia, Chen, Yong, Yu, Biao, Guo, Min, Fang, Pengfei, Fisher, David E, Wang, Jing. A unique hyperdynamic dimer interface permits small molecule perturbation of the melanoma oncoprotein MITF for melanoma therapy. CELL RESEARCH[J]. 2023, 33(1): 55-70, [9] Hei, Zhoufei, Fang, Pengfei. Sequential magnesium binding facilitates lysyl-tRNA synthetase to recognize ATP. BIOCHEMISTRY AND BIOPHYSICS REPORTS[J]. 2023, 33: 101426-, http://dx.doi.org/10.1016/j.bbrep.2023.101426.
[10] Liu, Yang, Chen, Yuting, Jiang, Jiheng, Chu, Xianglin, Guo, Qinglong, Zhao, Li, Feng, Feng, Liu, Wenyuan, Zhang, Xiaolong, He, Siyu, Yang, Peng, Fang, Pengfei, Sun, Haopeng. Development of highly potent and specific AKR1C3 inhibitors to restore the chemosensitivity of drug-resistant breast cancer. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY[J]. 2023, 247: http://dx.doi.org/10.1016/j.ejmech.2022.115013.
[11] Cheng, Yiyuan, Yi, Xuan, Zhang, Yan, He, Qingli, Chen, Dandan, Cao, Weiguo, Fang, Pengfei, Liu, Wen. Oxidase Heterotetramer Completes 1-Azabicyclo[3.1.0]hexane Formation with the Association of a Nonribosomal Peptide Synthetase.. J Am Chem Soc.[J]. 2023, 145(16): 8896-8907, [12] Lin, Yuqi, Shi, Qiqi, Yang, Guang, Shi, Fuchun, Zhou, Yang, Wang, Tongtong, Xu, Peng, Li, Peifeng, Liu, Zaizhou, Sun, Hanyin, Zhao, Zhixin, Ding, Ke, Wang, Zhen, Feng, Haizhong, Yu, Biao, Fang, Pengfei, Wang, Jing. A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB.. Proc Natl Acad Sci U S A[J]. 2023, 120(7): e2213670120-, [13] Yu, Tingting, Zhang, Yi, Zheng, WenQiang, Wu, Siqi, Li, Guoqiang, Zhang, Yong, Li, Niu, Yao, Ruen, Fang, Pengfei, Wang, Jian, Zhou, XiaoLong. Selective degradation of tRNA(Ser)(AGY) is the primary driver for mitochondrial seryl-tRNA synthetase-related disease. NUCLEIC ACIDS RESEARCH[J]. 2022, 50(20): 11755-11774, http://dx.doi.org/10.1093/nar/gkac1028.
[14] Baisen Zhong, Weiyu Peng, Shan Du, Bingyi Chen, Yajuan Feng, Xinfeng Hu, Qi Lai, Shujie Liu, ZhongWei Zhou, Pengfei Fang, Yan Wu, Feng Gao, Huihao Zhou, Litao Sun. Oridonin Inhibits SARS‐CoV‐2 by Targeting Its 3C‐Like Protease. SMALL SCIENCE[J]. 2022, 2(6): n/a-n/a, [15] siqi wu, li zheng, Zhoufei Hei, jing-bo zhou, guang li, peifeng li, jiayuan wang, hamid ali, 周小龙, jing wang, 房鹏飞. Human lysyl-tRNA synthetase evolves a dynamic structure that can be stabilized by forming complex. Cellular and Molecular Life Sciences[J]. 2022, [16] Cao, Xin, Du, Xiaojing, Jiao, Heng, An, Quanlin, Chen, Ruoxue, Fang, Pengfei, Wang, Jing, Yu, Biao. Carbohydrate-based drugs launched during 2000-2021. ACTA PHARMACEUTICA SINICA Bnull. 2022, 12(10): 3783-3821, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=7343026&detailType=1.
[17] Zhou, Jintong, Zheng, Li, Hei, Zhoufei, Li, Wei, Wang, Jing, Yu, Biao, Fang, Pengfei. Atomic Resolution Analyses of Isocoumarin Derivatives for Inhibition of Lysyl-tRNA Synthetase. ACS CHEMICAL BIOLOGY[J]. 2020, 15(4): 1016-1025, https://www.webofscience.com/wos/woscc/full-record/WOS:000526890700027.
[18] Wang, Yong, Zhou, JingBo, Zeng, QiYu, Wu, Siqi, Xue, MeiQin, Fang, Pengfei, Wang, EnDuo, Zhou, XiaoLong. Hearing impairment-associated KARS mutations lead to defects in aminoacylation of both cytoplasmic and mitochondrial tRNA(Lys). SCIENCE CHINA-LIFE SCIENCES[J]. 2020, 63(8): 1227-1239, http://lib.cqvip.com/Qikan/Article/Detail?id=7102433157.
[19] Zhou, Jintong, Huang, Zhenghui, Zheng, Li, Hei, Zhoufei, Wang, Zhiyong, Yu, Biao, Jiang, Lubin, Wang, Jing, Fang, Pengfei. Inhibition of Plasmodium falciparum Lysyl-tRNA synthetase via an anaplastic lymphoma kinase inhibitor. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(20): 11566-11576, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672456/.
[20] Zhou, XiaoLong, Chen, Yun, Zeng, QiYu, Ruan, ZhiRong, Fang, Pengfei, Wang, EnDuo. Newly acquired N-terminal extension targets threonyl-tRNA synthetase-like protein into the multiple tRNA synthetase complex. NUCLEIC ACIDS RESEARCH[J]. 2019, 47(16): 8662-8674, [21] Hei, Zhoufei, Wu, Siqi, Liu, Zaizhou, Wang, Jing, Fang, Pengfei. Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2019, 294(13): 4775-4783, http://dx.doi.org/10.1074/jbc.RA118.006356.
[22] Yu, Jing, Liu, Zaizhou, Liang, Yuanyuan, Luo, Feng, Zhang, Jie, Tian, Cuiping, Motzik, Alex, Zheng, Mengmeng, Kang, Jingwu, Zhong, Guisheng, Liu, Cong, Fang, Pengfei, Guo, Min, Razin, Ehud, Wang, Jing. Second messenger Ap(4)A polymerizes target protein HINT1 to transduce signals in Fc epsilon RI-activated mast cells. NATURE COMMUNICATIONS[J]. 2019, 10: [23] Jing Yu, Zaizhou Liu, Yuanyuan Liang, Feng Luo, Jie Zhang, Cuiping Tian, Alex Motzik, Mengmeng Zheng, Jingwu Kang, Guisheng Zhong, Cong Liu, Pengfei Fang, Min Guo, Ehud Razin, Jing Wang. Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells. NATURE COMMUNICATIONS[J]. 2019, 10(1): 1-12, http://dx.doi.org/10.1038/s41467-019-12710-8.
[24] Kong, Jiwon, Fang, Pengfei, Madoux, Franck, Spicer, Timothy P, Scampavia, Louis, Kim, Sunghoon, Guo, Min. High-Throughput Screening for Protein Synthesis Inhibitors Targeting Aminoacyl-tRNA Synthetases. SLAS DISCOVERY[J]. 2018, 23(2): 174-182, https://www.webofscience.com/wos/woscc/full-record/WOS:000423048900007.
[25] Fang, Pengfei, Guo, Min. Structural characterization of human aminoacyl-tRNA synthetases for translational and nontranslational functions. METHODS[J]. 2017, 113: 83-90, http://dx.doi.org/10.1016/j.ymeth.2016.11.014.
[26] Tao, Yeqing, Fang, Pengfei, Kim, Sunghoon, Guo, Min, Young, Nicolas L, Marshall, Alan G. Mapping the contact surfaces in the Lamin A: AIMP3 complex by hydrogen/deuterium exchange FT-ICR mass spectrometry. PLOS ONE[J]. 2017, 12(8): http://dx.doi.org/10.1371/journal.pone.0181869.
[27] Wang, Jing, Fang, Pengfei, Chase, Peter, Tshori, Sagi, Razin, Ehud, Spicer, Timothy P, Scampavia, Louis, Hodder, Peter, Guo, Min. Development of an HTS-Compatible Assay for Discovery of Melanoma-Related Microphthalmia Transcription Factor Disruptors Using AlphaScreen Technology. SLAS DISCOVERY[J]. 2017, 22(1): 58-66, https://www.webofscience.com/wos/woscc/full-record/WOS:000394206000007.
[28] Park, HaJeung, Gonzalez, Alex L, Yildirim, Ilyas, Tuan Tran, Lohman, Jeremy R, Fang, Pengfei, Guo, Min, Disney, Matthew D. Crystallographic and Computational Analyses of AUUCU Repeating RNA That Causes Spinocerebellar Ataxia Type 10 (SCA10). BIOCHEMISTRY[J]. 2015, 54(24): 3851-3859, https://www.webofscience.com/wos/woscc/full-record/WOS:000356989300014.
[29] Slade, Daniel J, Fang, Pengfei, Dreyton, Christina J, Zhang, Ying, Fuhrmann, Jakob, Rempel, Don, Bax, Benjamin D, Coonrod, Scott A, Lewis, Huw D, Guo, Min, Gross, Michael L, Thompson, Paul R. Protein Arginine Deiminase 2 Binds Calcium in an Ordered Fashion: Implications for Inhibitor Design. ACS CHEMICAL BIOLOGY[J]. 2015, 10(4): 1043-1053, [30] Fang, Pengfei, Guo, Min. The Nature's Clever Trick for Making Cyclic Dinucleotide. STRUCTUREnull. 2015, 23(5): 801-802, http://dx.doi.org/10.1016/j.str.2015.04.011.
[31] Mirando, Adam C, Fang, Pengfei, Williams, Tamara F, Baldor, Linda C, Howe, Alan K, Ebert, Alicia M, Wilkinson, Barrie, Lounsbury, Karen M, Guo, Min, Francklyn, Christopher S. Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor. SCIENTIFIC REPORTS[J]. 2015, 5: https://www.webofscience.com/wos/woscc/full-record/WOS:000359461300001.
[32] Fang, Pengfei, Yu, Xue, Jeong, Seung Jae, Mirando, Adam, Chen, Kaige, Chen, Xin, Kim, Sunghoon, Francklyn, Christopher S, Guo, Min. Structural basis for full-spectrum inhibition of translational functions on a tRNA synthetase. NATURE COMMUNICATIONS[J]. 2015, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000352585900001.
[33] Fang, Pengfei, Han, Hongyan, Wang, Jing, Chen, Kaige, Chen, Xin, Guo, Min. Structural Basis for Specific Inhibition of tRNA Synthetase by an ATP Competitive Inhibitor. CHEMISTRY & BIOLOGY[J]. 2015, 22(6): 734-744, http://dx.doi.org/10.1016/j.chembiol.2015.05.007.
[34] Rachmin, Inbal, Amsalem, Eden, Golomb, Eliahu, Beeri, Ronen, Gilon, Dan, Fang, Pengfei, Nechushtan, Hovav, Kay, Gillian, Guo, Min, Yiqing, Peter Li, Foo, Roger S Y, Fisher, David E, Razin, Ehud, Tshori, Sagi. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. INTERNATIONAL JOURNAL OF CARDIOLOGY[J]. 2015, 195: 85-94, http://dx.doi.org/10.1016/j.ijcard.2015.05.108.
[35] Pengfei Fang, Min Guo, LluxEDs Ribas de Pouplana. Evolutionary Limitation and Opportunities for Developing tRNA Synthetase Inhibitors with 5-Binding-Mode Classification. MDPI. 2015, http://oa.las.ac.cn/oainone/service/browseall/read1?ptype=JA&workid=JA202003190001950ZK.
[36] Kim, Dae Gyu, Lee, Jin Young, Kwon, Nam Hoon, Fang, Pengfei, Zhang, Qian, Wang, Jing, Young, Nicolas L, Guo, Min, Cho, Hye Young, Mushtaq, Ameeq Ul, Jeon, Young Ho, Choi, Jin Woo, Han, Jung Min, Kang, Ho Woong, Joo, Jae Eun, Hur, Youn, Kang, Wonyoung, Yang, Heekyoung, Nam, DoHyun, Lee, MiSook, Lee, Jung Weon, Kim, EunSook, Moon, Aree, Kim, Kibom, Kim, Doyeun, Kang, Eun Joo, Moon, Youngji, Rhee, Kyung Hee, Han, Byung Woo, Yang, Jee Sun, Han, Gyoonhee, Yang, Won Suk, Lee, Cheolju, Wang, MingWei, Kim, Sunghoon. Chemical inhibition of prometastatic lysyl-tRNA synthetase-laminin receptor interaction. NATURE CHEMICAL BIOLOGY[J]. 2014, 10(1): 29-U57, http://dx.doi.org/10.1038/NCHEMBIO.1381.
[37] Zhu, Yuwei, Dai, Jianxin, Zhang, Tiancheng, Li, Xu, Fang, Pengfei, Wang, Huajing, Jiang, Yongliang, Yu, Xiaojie, Xia, Tian, Niu, Liwen, Guo, Yajun, Teng, Maikun. Structural Insights into the Neutralization Mechanism of Monoclonal Antibody 6C2 against Ricin. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2013, 288(35): 25165-25172, http://dx.doi.org/10.1074/jbc.M113.480830.
[38] OfirBirin, Yifat, Fang, Pengfei, Bennett, Steven P, Zhang, HuiMin, Wang, Jing, Rachmin, Inbal, Shapiro, Ryan, Song, Jing, Dagan, Arie, Pozo, Jorge, Kim, Sunghoon, Marshall, Alan G, Schimmel, Paul, Yang, XiangLei, Nechushtan, Hovav, Razin, Ehud, Guo, Min. Structural Switch of Lysyl-tRNA Synthetase between Translation and Transcription. MOLECULAR CELL[J]. 2013, 49(1): 30-42, http://dx.doi.org/10.1016/j.molcel.2012.10.010.
[39] Wang, Jing, Fang, Pengfei, Schimmel, Paul, Guo, Min. Side Chain Independent Recognition of Aminoacyl Adenylates by the Hint1 Transcription Suppressor. JOURNAL OF PHYSICAL CHEMISTRY B[J]. 2012, 116(23): 6798-6805, https://www.webofscience.com/wos/woscc/full-record/WOS:000305356100024.
[40] Jones, Justin E, Slack, Jessica L, Fang, Pengfei, Zhang, Xuesen, Subramanian, Venkataraman, Causey, Corey P, Coonrod, Scott A, Guo, Min, Thompson, Paul R. Synthesis and Screening of a Haloacetamidine Containing Library To Identify PAD4 Selective Inhibitors. ACS CHEMICAL BIOLOGY[J]. 2012, 7(1): 160-165, https://www.webofscience.com/wos/woscc/full-record/WOS:000299241300017.
[41] Fang, Pengfei, Zhang, HuiMin, Shapiro, Ryan, Marshall, Alan G, Schimmel, Paul, Yang, XiangLei, Guo, Min. Structural context for mobilization of a human tRNA synthetase from its cytoplasmic complex. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2011, 108(20): 8239-8244, https://www.webofscience.com/wos/woscc/full-record/WOS:000290719600037.
[42] Kumar, Amit, Fang, Pengfei, Park, Hajeung, Guo, Min, Nettles, Kendall W, Disney, Matthew D. A Crystal Structure of a Model of the Repeating r(CGG) Transcript Found in Fragile X Syndrome. CHEMBIOCHEM[J]. 2011, 12(14): 2140-2142, https://www.webofscience.com/wos/woscc/full-record/WOS:000295227200008.
[43] Wang, Jing, Gossing, Michael, Fang, Pengfei, Zimmermann, Jana, Li, Xu, von Mollard, Gabriele Fischer, Niu, Liwen, Teng, Maikun. Epsin N-terminal homology domains bind on opposite sides of two SNAREs. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2011, 108(30): 12277-12282, http://dx.doi.org/10.1073/pnas.1013101108.
[44] Kumar, Arnit, Park, HaJeung, Fang, Pengfei, Parkesh, Raman, Guo, Min, Nettles, Kendall W, Disney, Matthew D. Myotonic Dystrophy Type 1 RNA Crystal Structures Reveal Heterogeneous 1 x 1 Nucleotide UU Internal Loop Conformations. BIOCHEMISTRY[J]. 2011, 50(45): 9928-9935, https://www.webofscience.com/wos/woscc/full-record/WOS:000296598100024.
[45] Xing, Li, Zhu, Yuwei, Fang, Pengfei, Wang, Jing, Zeng, Fuxing, Li, Xuesong, Teng, Maikun, Li, Xu. Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2011, 67: 727-729, http://dx.doi.org/10.1107/S1744309111014278.
[46] Fang, Pengfei, Li, Xu, Wang, Jing, Niu, Liwen, Teng, Maikun. Structural Basis for the Specificity of the GAE Domain of yGGA2 for Its Accessory Proteins Ent3 and Ent5. BIOCHEMISTRY[J]. 2010, 49(36): 7949-7955, http://dx.doi.org/10.1021/bi1010255.
[47] Fang, Pengfei, Li, Xu, Wang, Jing, Xing, Li, Gao, Yan, Niu, Liwen, Teng, Maikun. Crystal Structure of the Protein l-Isoaspartyl Methyltransferase from Escherichia coli. CELL BIOCHEMISTRY AND BIOPHYSICS[J]. 2010, 58(3): 163-167, http://dx.doi.org/10.1007/s12013-010-9103-2.
[48] Fang, Pengfei, Wang, Jing, Li, Xu, Guo, Min, Xing, Li, Cao, Xu, Zhu, Yi, Gao, Yan, Niu, Liwen, Teng, Maikun. Crystallization and preliminary X-ray analysis of Escherichia coli RNase G. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2009, 65: 586-588, http://dx.doi.org/10.1107/S1744309109015802.