电子邮件： fangys@sioc.ac.cn
通信地址： 上海市海科路100号
邮政编码： 201203
研究领域
方燕姗研究员课题组隶属于中国科学院上海有机化学研究所生物与化学交叉研究中心，课题组主要研究方向为神经损伤和神经退行性疾病中的神经退化（neurodegeneration）。
神经退化是急性神经损伤如脊髓损伤和脑创伤以及很多与衰老相关的慢性神经退行性疾病如老年痴呆症(AD)、帕金森氏症(PD)、亨廷顿氏症(HD)、多发性硬化症(MS)、肌萎缩性脊髓侧索硬化症(ALS)等疾病中的常见病变。由于人类的中枢神经在受损或变性坏死后再生能力极其有限，如何保护受损或患病的神经，预防、延缓、阻止乃至逆转神经退化是神经生物学领域的一个重要前沿课题。
方燕姗研究员多年来一直致力于细胞和分子神经生物学研究，特别是利用模式生物结合细胞生物学、分子遗传学、高分辨率活体显微成像、现代蛋白质组学等技术对神经退化的细胞和分子机理进行探究。其科研内容主要包括以下两大方向：（1）探寻急性神经损伤中神经轴突退化变性的分子机理，寻找保护受损神经、促进神经恢复的新基因、新分子和新药物靶点；（2）研究慢性神经退行性疾病如肌萎缩性脊髓侧索硬化症(ALS)、阿尔兹海默症(AD)、帕金森氏症(PD)等的分子基础，揭示神经细胞退化的调控机制，以此推动疾病诊断治疗的生物标志物和新型药物的研发。
教育背景
工作经历
2013-09 ~ 今 中国科学院上海有机化学研究所 生物与化学交叉研究中心 研究员、课题组长
2008-06 ~ 2013-08 美国霍华德休斯医学研究院(Howard Hughes Medical Institute) Research Assoicate
2008-01 ~ 2008-05 美国宾夕法尼亚大学(University of Pennsylvania) 博士后
教授课程
获奖情况
入选国家高层次人才 (青年) 计划，2014
入选国家863计划青年科学家项目，2014
第七届世界神经科学大会ACS青年科学家旅行资助奖，Australasian Chronobiology Society, 2007
北京市优秀毕业生，中华人民共和国教育部, 2002
光华特等奖学金，2001
联邦制药特等奖学金，1999
北京大学一等奖学金，1998, 2000
专利成果
代表论著
2022:
Deng X, Sun X, Yue W, Duan Y, Hu R, Zhang K, Ni J, Cui J, Wang Q, Chen Y, Li A# and FANG Y#. (2022) “CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.” Journal of Cell Biology, 221(1):e202103033.
Hsp70 exhibits a liquid-liquid phase separation ability and chaperones condensed FUS against amyloid aggregation. Li Y, Gu J, Wang C, Hu J, Zhang S, Liu C, Zhang S, FANG Y, Li D. (2022) iScience. 25(6):104356.
The mouse nicotinamide mononucleotide adenylyltransferase chaperones diverse pathological amyloid client proteins. (2022) Huang C, Lu J, Ma X, Qiang J, Wang C, Liu C, FANG Y, Zhang Y, Jiang L, Li D, Zhang S. J Biol Chem. 298(5):101912.
2021:
Zhang K, Jiang M and FANG Y. (2021) “The drama of Wallerian degeneration: The cast, crew and script.” Annual Review of Genetics, 55:93-113.
Gu J, Wang C, Hu R, Li Y, Zhang S, Sun Y, Wang Q, Li D, FANG Y# and Liu C#. (2021) “Hsp70 chaperones TDP-43 in dynamic, liquid-Like phase and prevents it from amyloid aggregation.” Cell Research, 31(9):1024-1027.
Zhang K, Wang Q, Liang Y, Yan Y, Wang H, Cao X, Shan B, Zhang Y#, Li A# and FANG Y#. (2021) “Quantitative proteomic analysis of mouse sciatic nerve reveals post-injury upregulation of ADPGK promoting macrophage phagocytosis.” Frontiers in Molecular Neuroscience, 14:777621.
Ni J, Ren Y, Su T, Zhou J, Fu C, Yi Lu, Li D, Zhao J, Li Y, Zhang Y, FANG Y, Liu N, Geng Y# and Chen Y#. (2021) “Loss of TDP-43 function underlies hippocampal and cortical synaptic deficits in TDP-43 proteinopathies.” Molecular Psychiatry, (DOI: 10.1038/s41380-021-01346-0. Online ahead of print).
Wang H, Zhang Y, Zeng K, Qiang J, Cao Y, Li Y, FANG Y, Zhang Y# and Chen Y#. (2021) “Selective mitochondrial protein labeling enabled by biocompatible photocatalytic reactions inside live cells.” JACS Au, 1(7):1066-1074.
2020:
Wang C, Duan Y, Duan G, Wang Q, Zhang K, Deng X, Qian B, Gu J, Ma Z, Zhang S, Guo L, Liu C# and FANG Y#. (2020) “Stress induces dynamic, cytotoxicity-antagonizing TDP-43 nuclear bodies via paraspeckle lncRNA NEAT1-mediated liquid-liquid phase separation.” Molecular Cell, 79(3):443-458.e7. (Highlighted with commentary by Malik and Barmada in Molecular Cell).
Gu J, Liu Z, Zhang S, Li Y, Xia W, Wang C, Xiang H, Liu Z, Tan L, FANG Y, Liu C# and Li D#. (2020) “Hsp40 proteins phase separate to chaperone the assembly and maintenance of membraneless organelles.” Proc Natl Acad Sci U S A, 117(49):31123-31133.
Sun Y, Zhao K, Xia W, Feng G, Gu J, Ma Y, Gui X, Zhang X, FANG Y, Sun B, Wang R, Liu C# and Li D#. (2020) The nuclear localization sequence mediates hnRNPA1 amyloid fibril formation revealed by cryoEM structure. Nat Communications, 11(1):6349.
Ma X, Zhu Y, Lu J, Xie J, Li C, Shin WS, Qiang J, Liu J, Dou S, Xiao Y, Wang C, Jia C, Long H, Yang J, FANG Y, Jiang L, Zhang Y, Zhang S, Zhai RG#, Liu C# and Li D#. (2020) “Nicotinamide mononucleotide adenylyltransferase uses its NAD(+) substrate-binding site to chaperone phosphorylated Tau.” Elife, 9. pii:e51859.
2019:
Duan Y, Du A, Gu J, Duan G, Wang C, Gui X, Ma Z, Qian B, Deng X, Zhang K, Sun L, Tian K, Zhang Y, Jiang H, Liu C# and FANG Y#. (2019) “PARylation regulates stress granule dynamics, phase separation, and neurotoxicity of disease-related RNA-binding proteins.” Cell Research, 29(3):233-247.
Wang H, Wang X, Zhang K, Wang Q, Cao X, Wang Z, Zhang S, Li A# Liu K# and FANG Y#. (2019) “Rapid depletion of ESCRT protein Vps4 underlies injury-induced autophagic impediment and Wallerian degeneration.” Science Advances, 5(2):eaav4971.
Liu C# and FANG Y#. (2019) New insights of poly(ADP-ribosylation) in neurodegenerative diseases: a focus on protein phase separation and pathologic aggregation. Biochemical Pharmacology, 167:58-63.
Yang L, Cao Y, Zhao J, FANG Y, Liu N# and Zhang Y#. (2019) “Multidimensional proteomics identifies declines in protein homeostasis and mitochondria as early signals for normal aging and age-associated disease in Drosophila”. Mol Cell Proteomics, 8(10):2078-2088.
2018:
Sun X, Duan Y, Qin C, Li JC, Duan G, Deng X, Ni J, Cao X, Xiang K, Tian K, Chen CH, Li A# and FANG Y#. (2018) “Distinct multilevel misregulations of Parkin and PINK1 revealed in cell and animal models of TDP-43 proteinopathy.” Cell Death & Disease, 9(10):953.
2017:
Cao X, Wang H, Wang Z, Wang Q, Zhang S, Deng Y and FANG Y. (2017) “In vivo imaging reveals mitophagy independence in the maintenance of axonal mitochondria during normal aging.” Aging Cell, 16(5):1180-1190.
2015:
Cao X and FANG Y. (2015) “Transducing oxidative stress to death signals in neurons”. Journal of Cell Biology, 211(4):741-743.
FANG Y# and Boinini NM#. (2015) “Hope on the fruit fly - the Drosophila wing paradigm of axon injury”. Neural Regeneration Research, 10(2):173-175.
2013 and before:
FANG Y, Soares L and Bonini NM. (2013) “Design and implementation of in vivo imaging of neural injury responses in the adult Drosophila wing.” Nature Protocols, 8(4): 810-819.
Garbe DS, FANG Y, Zheng X, Sowcik M, Anjum R, Gygi SP and Sehgal A. (2013) “Cooperative interaction between phosphorylation sites on PERIOD maintains circadian period in Drosophila.” PLoS Genetics, 9(9): e1003749.
FANG Y and Boinini NM. (2012) “Axon degeneration and regeneration: insights from Drosophila models of nerve injury.” Annual Review of Cell and Developmental Biology, 28:575-597.
FANG Y, Soares L, Teng X, Geary M and Bonini NM. (2012) “A novel Drosophila model of nerve injury reveals an essential role of Nmnat in maintaining zxonal integrity.” Current Biology, 22(7):590-595. (Highlighted with commentary by Wang and Barres in Current Biology; Recommended by Faculty of 1000; Featured in ScienceDaily, etc.)
FANG Y and Sehgal A. (2010) “Protein phosphatases and circadian clocks.” in the Handbook of Cell Signaling, 2nd Edition, eds. Bradshaw, R. A. & Dennis, E. A. Oxford:Academic Press, pp. 877-882.
Wu Y, Bolduc FV, Bell K, Tully T, FANG Y, Sehgal A and Fischer JA. (2008) “A Drosophila model for Angelman syndrome.” Proc Natl Acad Sci U S A, 105(34):12399-12404.
FANG Y, Sathyanarayanan S and Sehgal A. (2007) “Post-translational regulation of the Drosophila circadian clock requires protein phosphatase 1 (PP1).” Genes and Development, 21(12):1506-1518.
Sehgal A, Joiner W, Crocker A, Koh K, Sathyanarayanan S, FANG Y, Wu M, Williams J and Zheng X. (2007) “Molecular analysis of sleep:wake cycles in Drosophila.” Cold Spring Harb Symp Quant Biol, 72:557-564.
Stein JM, Bergman W, FANG Y, Davison L, Brensinger C, Robinson MB, Hecht NB and Abel T. (2006) “Behavioral and neurochemical alterations in mice lacking the RNA-binding protein Translin.” Journal of Neuroscience, 26(8):2184-2196.