基本信息
冯银刚  男  博导  中国科学院青岛生物能源与过程研究所
电子邮件: fengyg@qibebt.ac.cn
通信地址: 青岛市崂山区松岭路189号
邮政编码: 266101

研究领域

1.能源微生物的分子生理机制与合成生物学应用。主要研究对象包括产纤维小体细菌、产溶剂细菌、产油微藻等。

2.工业酶催化机制与酶工程。主要研究对象包括糖苷水解酶、环氧化物水解酶、P450酶、聚酮合酶等。


招生信息

   
招生专业
071005-微生物学
招生方向
微生物遗传改造与代谢工程

教育背景

1999-09--2004-06   中国科学院生物物理研究所   博士学位
1995-09--1999-06   南京大学物理学系   学士学位
学历
-- 研究生
学位
-- 博士

工作经历

   
工作简历
2011-06~现在, 中国科学院青岛生物能源与过程研究所, 研究员
2006-07~2011-05,中国科学院生物物理研究所, 副研究员
2004-07~2006-06,北京大学化学与分子工程学院, 博士后
社会兼职
2020-01-01-2022-12-31,Bioresources and Bioprocessing编委,
2014-04-19-今,中国生物物理学会-生物磁共振分会理事,

出版信息

   
发表论文
(1) Structural insight into a GH1 β-glucosidase from the oleaginous microalga, Nannochloropsis oceanica., Int. J. Biol. Macromol., 2021, 通讯作者
(2) Bacillaenes: decomposition trigger point and biofilm enhancement in Bacillus., ACS Omega, 2021, 第 6 作者
(3) Structural basis for the DNA-binding activity of human ARID4B Tudor domain., J. Biol. Chem., 2021, 通讯作者
(4) Structural basis for selective oxidation of phosphorylated ethylphenols by cytochrome P450 monooxygenase CreJ., Appl. Environ. Microbiol., 2021, 通讯作者
(5) NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF from Clostridium thermocellum., Biomol. NMR Assign., 2021, 通讯作者
(6) 纤维小体在合成生物学中的应用研究进展, 合成生物学, 2021, 通讯作者
(7) Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world., Biotechnol. Adv., 2020, 通讯作者
(8) The chaperone Hsp70 is a BH3 receptor activated by the pro-apoptotic BIM to stabilize anti-apoptotic clients., J. Biol. Chem., 2020, 第 7 作者
(9) Structural insights into transcription initiation from de novo RNA synthesis to transitioning into elongation., iScience, 2020, 第 3 作者
(10) Structural basis of specificity for carboxyl-terminated acyl donors in a bacterial acyltransferase., J. Am. Chem. Soc., 2020, 第 4 作者
(11) Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules., Sci. Adv., 2020, 通讯作者
(12) Targeting the allosteric pathway that interconnects the core-functional scaffold and the distal phosphorylation sites for specific dephosphorylation of Bcl-2., J. Med. Chem., 2020, 第 6 作者
(13) Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum., Biomol. NMR Assign., 2019, 通讯作者
(14) Resonance assignments for the tandem PWWP-ARID domains of human RBBP1., Biomol. NMR Assign., 2019, 通讯作者
(15) Changes in peptidomes and Fischer ratios of corn-derived oligopeptides depending on enzyme hydrolysis approaches, Food Chemistry, 2019, 第 3 作者
(16) Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization., Biotechnol. Biofuels, 2019, 第 3 作者
(17) Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity., Arch. Biochem. Biophys., 2019, 通讯作者
(18) An effective strategy for identification of highly unstable bacillaenes., J. Nat. Prod., 2019, 第 8 作者
(19) Alternative σI/anti-σI factors represent a unique form of bacterial σ/anti-σ complex., Nucleic Acids Res., 2019, 通讯作者
(20) Phytohormones as stimulators to improve arachidonic acid biosynthesis in Mortierella alpine., Enzyme Microbial Technol., 2019, 第 3 作者
(21) Enantiomeric tartaric acid production using cis-epoxysuccinate hydrolase: history and perspectives., Molecules, 2019, 通讯作者
(22) Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum., Microbial Biotechnol., 2018, 第 2 作者
(23) Structural insight into the catalytic mechanism of a cis-epoxysuccinate hydrolase producing enantiomerically pure D(-)-tartaric acid., Chem. Commun., 2018, 通讯作者
(24) Regulation of biomass degradation by alternative σ factors in cellulolytic clostridia., Sci. Rep., 2018, 第 6 作者
(25) Firmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique +1 transcriptional slippage., Biotechnol. Biofuels, 2018, 通讯作者
(26) Artificial creation of Chlorella pyrenoidosa mutants for economic sustainable food production., Bioresour. Technol., 2018, 第 4 作者
(27) PBN11-8, a cytotoxic polypeptide purified from marine Bacillus, suppresses invasion and migration of human hepatocellular carcinoma cells by targeting focal adhesion kinase pathways., Polymers, 2018, 第 10 作者
(28) Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum., Biotechnol. Biofuels, 2018, 通讯作者
(29) The spatial proximity effect of beta-glucosidase and cellulosomes on cellulose degradation., Enzyme Microbial Technol., 2018, 第 3 作者
(30) Heavy ion mutagenesis combined with triclosan screening provides a new strategy for improving the arachidonic acid yield in Mortierella alpine., BMC Biotechnol., 2018, 第 4 作者
(31) Solution structure of an archaeal DUF61 family protein SSO0941 encoded by a gene in the operon of box C/D RNA protein complexes., J. Struct. Biol., 2018, 通讯作者
(32) Structural insight into the stabilizing effect of O-glycosylation, Biochemistry, 2017, 通讯作者
(33) Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum., Biotechnol. Biofuels, 2017, 第 6 作者
(34) Response mechanism of the docosahexaenoic acid producer Aurantiochytrium under cold stress., Algal Res., 2017, 第 5 作者
(35) Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system., Proc. Natl. Acad. Sci. USA, 2017, 通讯作者
(36) Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpine., BMC Biotechnol., 2017, 第 2 作者
(37) Backbone and side-chain 1H, 15N and 13C resonance assignments of two Sac10b family members Mvo10b and Mth10bTQQA from archaea., Biomol. NMR Assign., 2017, 通讯作者
(38) Structural insights into the substrate specificity of a glycoside hydrolase family 5 lichenase from Caldicellulosiruptor sp. F32., Biochem. J., 2017, 通讯作者
(39) Compatible topologies and parameters for NMR structure determination of carbohydrates by simulated annealing., PLoS One, 2017, 第 1 作者
(40) Resonance assignments of a VapC family toxin from Clostridium thermocellum., Biomol. NMR Assign., 2016, 通讯作者
(41) New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16., J. Mol. Cell Biol., 2016, 第 6 作者
(42) Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116., Algal Res., 2016, 第 4 作者
(43) Deactivation of Mcl-1 by dual-function small-molecule inhibitors targeting the Bcl-2 homology 3 domain and facilitating Mcl-1 ubiquitination., Angew. Chem. Int. Ed., 2016, 第 4 作者
(44) 细菌中I型毒素-抗毒素系统的研究进展, 生物化学与生物物理进展, 2016, 通讯作者
(45) Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation., Appl. Microbiol. Biotechnol., 2016, 通讯作者
(46) NMR structure note: the C-terminal region of human eukaryotic elongation factor 1Bδ., J. Biomol. NMR, 2016, 通讯作者
(47) Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin., FEBS Lett., 2016, 通讯作者
(48) Bcl-2/MDM2 dual inhibitors based on universal pyramid-like α-helical mimetics., J. Med. Chem., 2016, 第 3 作者
(49) 利用伯克霍尔德氏菌脂肪酶富集裂壶藻油脂中的DHA, 食品科学, 2015, 通讯作者
(50) Evolutionarily conserved binding of translationally-controlled tumor protein to eukaryotic elongation factor 1B. , J. Biol. Chem., 2015, 通讯作者
(51) Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions. , Algal Res., 2015, 通讯作者
(52) Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum, Biomol. NMR Assign., 2015, 通讯作者
(53) 1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from photosynthetic microalga Nannochloropsis oceanica., Biomol. NMR Assign., 2015, 通讯作者
(54) High production of squalene using a newly isolated yeast-like strain Pseudozyma sp. SD301., J. Agric. Food Chem., 2015, 第 4 作者
(55) Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions., Sci. Rep., 2015, 第 4 作者
(56) Revisiting the NMR solution structure of the Cel48S type-I dockerin module from Clostridium thermocellum reveals a cohesin-primed conformation., J. Struct. Biol., 2014, 通讯作者
(57) Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation, Genes Dev., 2014, 第 4 作者
(58) 热纤梭菌中心代谢产物的归属及初步代谢组分析, 波谱学杂志, 2014, 第 3 作者
(59) 裂殖壶菌SD116油脂的胞内核磁共振研究, 生物物理学报, 2014, 通讯作者
(60) Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system., App. Microbiol. Biotechnol. , 2014, 通讯作者
(61) Retinoblastoma-binding protein 1 has an interdigitated double Tudor domain with DNA-binding activity., J. Biol. Chem., 2014, 通讯作者
(62) Structural and biochemical characterization reveals LysGH15 as an unprecedented EF-hand-like calcium-binding phage lysin, PLoS Pathog. , 2014, 第 2 作者
(63) Mechanism of the Rpn13-induced activation of Uch37, Protein Cell , 2014, 通讯作者
(64) Type AII lantibiotic bovicin HJ50 with a rare disulfide bond: structure, structure-activity relationships and mode of action, Biochem. J. , 2014, 第 1 作者
(65) Two-face, two-turn α-helix mimetics based on a cross-acridine scaffold: analogues of the Bim BH3 domain.,  ChemBioChem , 2014, 第 3 作者
(66) The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons. , Biotechnol. Biofuels , 2014, 第 3 作者
(67) Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold., Biochem. Biophys. Res. Commun., 2014, 通讯作者
(68) Metabolic adaption of ethanol-tolerant Clostridium thermocellum., PLoS One, 2013, 第 3 作者
(69) Anti-apoptosis proteins Mcl-1 and Bcl-xL have different p53-binding profiles., Biochemistry, 2013, 通讯作者
(70) An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., PLoS Biol., 2013, 第 3 作者
(71) Separation and quantification of water-soluble cellular metabolites in Clostridium thermocellum using liquid chromatography-isotope dilution tandem mass spectrometry., Anal. Lett., 2013, 通讯作者
(72) NMR structure note: a PilT N-terminus domain protein SSO1118 from hyperthemophilic archaeon Sulfolobus solfataricus P2. , J. Biomol. NMR, 2013, 通讯作者
(73) Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824, Biomol. NMR Assign., 2013, 第 4 作者
(74) Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress, Bioresour. Technol. , 2013, 第 4 作者
(75) Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116. , J. Oleo Sci., 2013, 第 3 作者
(76) Modeling of the [E43S]SNase-ssDNA-Cd2+ complex: structural insight into the action of nuclease on ssDNA, Arch. Biochem. Biophys. , 2013, 第 2 作者
(77) Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts., Proc. Natl. Acad. Sci. USA , 2013, 通讯作者
(78) Novel soluble myeloid cell leukemia sequence 1 (Mcl-1) inhibitor (E,E)-2-(benzylaminocarbonyl)-3-styrylacrylonitrile (4g) developed using a fragment-based approach. , Eur. J. Med. Chem., 2013, 通讯作者
(79) The archaeal Sac10b protein family: conserved proteins with divergent functions, Curr. Protein Pept. Sci., 2012, 通讯作者
(80) 古菌蛋白质SSO6904的双体形式的NMR鉴定, Dimer form of archaeal protein SSO6904 identified by NMR, 波谱学杂志, 2012, 通讯作者
(81) Structural insight into recognition of methylated histone tails by retinoblastoma-binding protein 1, J. Biol. Chem., 2012, 通讯作者
(82) NMR studies of the interaction between human programmed cell death 5 and human p53, Biochemistry, 2012, 第 2 作者
(83) Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin, Protein Cell , 2012, 第 4 作者
(84)  Targeted gene engineering in Clostridium cellulolyticum H10 without methylation., J. Microbiol. Methods, 2012, 通讯作者
(85) NMR structure note: human esophageal cancer-related gene 2, J. Biomol. NMR, 2012, 第 1 作者
(86) Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases, PLoS One, 2012, 通讯作者
(87) Efficiency and stability enhancement of cis-epoxysuccinic acid hydrolase by fusion with a carbohydrate binding module and immobilization onto cellulose, Appl. Biochem. Biotechnol., 2012, 通讯作者
(88) High yield recombinant expression, characterization and homology modeling of two types of cis-epoxysuccinic acid hydrolases., Protein J. , 2012, 通讯作者
(89) An anthraquinone scaffold for putative, two-face Bim BH3 α-helix mimicries, J. Med. Chem., 2012, 通讯作者
(90) Molecular basis of Wnt activation via the DIX-domain protein Ccd1, J. Biol. Chem., 2011, 第 4 作者
(91) Resonance assignments of a putative PilT N-terminus domain protein SSO1118 from hyperthermophilic archaeon Sulfolobus solfataricus P2., Biomol. NMR Assign. , 2011, 通讯作者
(92) Protein-protein complexation in bioluminescence, Protein Cell, 2011, 第 2 作者
(93) NMR derived topology of a GFP-photoprotein energy transfer complex, J. Biol. Chem., 2010, 第 1 作者
(94) Binding of NADPH destabilizes the iron-sulfur clusters of human mitoNEET. , Biochemistry, 2010, 第 3 作者
(95) Crystal Structure of the crenarchaeal conserved chromatin protein Cren7 and double-stranded DNA complex., Protein Sci., 2010, 第 1 作者
(96) Importance of the C-terminal loop L137-S141 for the folding and folding stability of staphylococcal nuclease, Biochemistry, 2010, 第 2 作者
(97) Solution structure and calcium binding of protein SSO6904 from the hyperthermophilic archaeon Sulfolobus solfataricus, Proteins, 2010, 通讯作者
(98) The native-like interactions between SNase121 and SNase(111-143) fragments induce the recovery of their native-like structures and the ability to degrade DNA, Biochemistry, 2009, 第 1 作者
(99) Structure-function correlation of human programmed cell death 5 protein, Arch. Biochem. Biophys. , 2009, 第 3 作者
(100)  Cloning, expression and purification of DNA binding protein Mvo10b from Methanococcus voltae. , Protein Expr. Purif. , 2009, 第 3 作者
(101) Solution structure of synbindin atypical PDZ domain and interaction with syndecan-2. , Protein Peptide Lett., 2009, 第 1 作者
(102) Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b. , Arch. Biochem. Biophys. , 2009, 第 2 作者
(103) A stabilizing alpha/beta-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. , Biochemistry, 2008, 第 4 作者
(104) Mapping the putative binding site for uPA protein in Esopageal Cancer Related Gene 2 by heteronuclear NMR method. , Arch. Biochem. Biophys. , 2008, 第 2 作者
(105)  Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. , Nucleic Acid Res. , 2008, 第 1 作者
(106) Solution structure and mapping of a very weak calcium-binding site of human translationally controlled tumor protein by NMR. , Arch. Biochem. Biophys., 2007, 第 1 作者
(107) Folding of the C-terminal fragment V111-D143 of staphylococcal nuclease in aqueous solution., Protein Peptide Lett., 2007, 第 4 作者
(108) Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease. , Biophys. J., 2007, 第 3 作者
(109) 谷氧还蛋白系统及其对细胞氧化还原态势的调控, 生物物理学报, 2007, 第 2 作者
(110) Structural insight into poplar glutaredoxin C1 with a bridging iron-sulfur cluster at the active site, Biochemistry, 2006, 第 1 作者
(111) The N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable alpha-helix having unique electrostatic potential character. , Biochem. J., 2005, 第 4 作者
(112) Letter to the Editor: 1H, 15N, and 13C Resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides., J. Biomol. NMR, 2005, 第 1 作者
(113) Searching for folding initiation sites of staphylococcal nuclease: a study of N-terminal short fragments. , Biopolymers, 2004, 第 3 作者
(114) Human programmed cell death 5 protein has a helical-core and two dissociated structural regions., Biochem. Biophys. Res. Commun. , 2004, 第 2 作者
(115) Two conformations of archaeal Ssh10b: the origin of its temperature-dependent interaction with DNA., J. Biol. Chem., 2003, 通讯作者
(116) Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy., J. Mol. Biol., 2003, 第 1 作者
(117) Letters to the Editor: 1H, 15N, and 13C resonance assignments and secondary structure of the Ssh10b from hyperthermophilic archaeon Sulfolobus shibatae. , J. Biomol. NMR, 2002, 第 3 作者

科研活动

   
科研项目
( 1 ) 梭菌纤维小体调控因子信号传导的结构与分子机制的核磁共振研究, 主持, 国家级, 2017-01--2020-12
( 2 ) 溶纤维假拟杆菌生物质传感巨型调控元的机制解析, 主持, 国家级, 2017-01--2019-12
( 3 ) 手性酶催化的静态分子结构与催化机制研究, 主持, 市地级, 2019-01--2021-12
( 4 ) 热纤梭菌寡糖转运与纤维小体调控偶联机制研究, 主持, 国家级, 2021-01--2024-12
参与会议
(1)厌氧细菌复杂纤维小体的调控机制   2020中国微生物学会年会   2020-10-23
(2)Structural and functional insights into novel bacterial sigma/anti-sigma factors for cellulosome regulation   第三届全国生物磁共振研讨会   2019-05-29
(3)热纤梭菌纤维小体——乐高积木式分子机器的诱导调控机制   2018年中国微生物学会学术年   2018-10-19
(4)The pH-dependent interaction of cellulosomal assembling modules from Clostridium acetobutylicum   第十五届中国暨国际生物物理大会   2017-11-03
(5)Structure and Function of Transmembrane Extracytoplasmic Function (ECF) Anti-sigma Factors for Cellulosome Regulation in Clostridium thermocellum   第十四届中国暨国际生物物理大会   2015-11-19
(6)NMR Structures of Cellulosomal Cohesin and Dockerin from Clostridium acetobutylicum    第十九届国际磁共振学会会议   2015-08-17
(7)热纤梭菌中anti-sigma因子的核磁共振研究   第十八届全国波谱学学术会议   2014-10-10
(8)癌症抑制相关蛋白ARID4A的结构与相互作用研究   中国生物物理学会生物磁共振专业委员会成立大会暨第一届全国生物磁共振研讨会   2014-04-18