RNA沉默是一种在动植物中广泛存在的抵抗外源RNA和病毒的适应性免疫反应机制。能产生信号并系统运输是植物RNA沉默最重要的特征，即系统RNA沉默，也是植物抗病毒最关键的一环。另外，siRNA参与DNA甲基化和染色质重塑，使RNA沉默成为表观遗传学 (Epigenetic)研究领域的重要课题。本研究组以模式植物拟南芥、烟草和重要的经济作物棉花为寄主材料，以病毒和真菌为病原微生物，围绕植物RNA沉默和miRNA调控途径，以及其他受病原影响的调控途径开展病原微生物－寄主的相互作用研究，剖析植物致病机理。目前开展以下两方面的研究:

1、植物RNA沉默的分子机制及其抗病应用研究；

2、植物与真菌的互作机理及维管束真菌病害的防控策略研究。

   

071007-遗传学

植物RNA沉默及其抗病应用，真菌致病性研究

1993-06--1996-06   西班牙马德里大学   博士
1983-09--1987-07   中山大学   学士

中山大学生物学，学士

西班牙马德里大学， 博士

1992-1993 西班牙马德里大学"Severo Ochoa"分子生物学中心，Guest Investigator
1997-1998 西班牙马德里大学国家生物工程中心（CSIC）博士后

   

2004-07~现在, 中国科学院微生物研究所, 中国科学院"****"，研究员
2003-01~2004-06,新加坡国立大学淡马锡生命科学研究院, Acting PI
1998-07~2003-01,新加坡国立大学分子农业生物学院/淡马锡生命科学研究院, Research Fellow
1997-07~1998-06,西班牙马德里大学国家生物工程中心（CSIC）, 博士后
1992-01~1993-05,西班牙马德里大学"Severo Ochoa"分子生物学中心, Guest Investigator
1987-09~1991-12,中山大学生物工程中心, 助教

2018-01-01-今,PBJ Editorial Board, Editorial Board
2018-01-01-今,MPP Editorial Board, Editorial Board
2017-11-01-今,生物工程学报, 副主编
2017-08-01-今,植物基因组学国家重点实验室学术委员会, 副主任
2017-05-31-今,中国科学院微生物研究所招生小组, 成员
2014-12-31-2017-12-30,Molecular Plant Pathogens 编委, Editorial Board
2014-01-02-今,中国科学院微生物研究所学术道德与权益委员会, 委员
2014-01-01-2018-12-31,中国植物病理学会第十届理事会, 理事
2014-01-01-今,中国科学院微生物研究所第一届研究组长委员会, 委员
2013-12-31-今,BMC genomics 编委, Associate Editor
2013-11-04-今,中国科学院微生物研究所学术委员会, 委员
2013-01-31-今,PLoS Pathogens 编委, Associate Editor
2013-01-01-今,中国科学院微生物研究所第八届学位评定委员会, 委员
2012-12-31-2015-12-30,Molecular Plant-Microbe Interact ions编委,
2011-12-21-今,植物基因组学国家重点实验室第三届学术委员会, 委员

信号转导
微生物学系列讲座
信号传导

   

（1） 中国科学院大学教育教学成果奖, 二等奖, 研究所（学校）, 2019
（2） 所长奖教金, 研究所（学校）, 2017
（3） 中国科学院特聘核心骨干, 院级, 2017
（4） 中国植物病理学会会士, 其他, 2015
（5） 中国科学院特聘骨干人才, 院级, 2015
（6） 中国科学院大学岗位教授, 院级, 2015
（7） 政府特殊津贴, 国家级, 2015
（8） 伊品生物奖教金, 研究所（学校）, 2015
（9） 优秀共产党员, 部委级, 2015
（10） 朱李月华优秀教师奖, , 院级, 2012
（11） 所长奖教金, , 研究所（学校）, 2010
（12） 所长奖教金, 特等奖, 研究所（学校）, 2008
（13） 国家杰出青年科学基金, 特等奖, 部委级, 2005

（ 1 ） 一种培育抗黄瓜花叶病毒植物的方法, 发明, 2010, 第 1 作者, 专利号: ZL 2007 1 0099649.3
（ 2 ） 来自大丽轮枝菌的致病相关蛋白VdGARP1及其编码基因, 发明, 2013, 第 1 作者, 专利号: ZL 2011 1 0090424.8
（ 3 ） RNA诱导的沉默复合体介导的剪切位点及其应用, 发明, 2010, 第 1 作者, 专利号: ZL 2008 1 0144424.X
（ 4 ） 来自大丽轮枝菌的分泌型激发子蛋白及其编码基因与应用, 发明, 2012, 第 1 作者, 专利号: 201210006128.X
（ 5 ） 一种大丽轮枝菌的致病相关蛋白VdSENP1及其编码基因, 发明, 2013, 第 1 作者, 专利号: 201310424509.4
（ 6 ） 培育抗黄萎病的棉花的方法, 发明, 2015, 第 1 作者, 专利号: 201510257627.X
（ 7 ） 与植物生物量相关的miRNA及其相关生物材料与应用, 发明, 2016, 第 1 作者, 专利号: 201410157643.7
（ 8 ） 一种高效基因敲除载体的构建方法及构建的工程菌, 发明, 2015, 第 1 作者, 专利号: 201510430397.2
（ 9 ） 大丽轮枝菌的致病基因、蛋白及其应用, 发明, 2015, 第 1 作者, 专利号: 2015109826933
（ 10 ） 大丽轮枝菌的致病基因、蛋白及其应用, 发明, 2015, 第 1 作者, 专利号: 2015109818975
（ 11 ） 一种大丽轮枝菌的致病相关蛋白VdSENP1及其编码基因, 发明, 2017, 第 1 作者, 专利号: ZL201310424509.4
（ 12 ） 蛋白质及基因的应用、以及重组载体、表达盒、重组菌及构建方法, 发明, 2017, 第 1 作者, 专利号: 201710487688.4
（ 13 ） 培育抗黄萎病的棉花的方法, 发明, 2018, 第 1 作者, 专利号: ZL201510257627.X
（ 14 ） 重组载体及其制备方法、以及提高蔬菜作物芜菁花叶病毒抗性和/或产量的方法, 发明, 2018, 第 1 作者, 专利号: 2018109285758

   

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[14] Xiaolan Liu, Jianhua Zhao, HuiShan Guo. IBM1-dependent H3K9 demethylation enables self-silencing of an exogenous silencer for the non-cell autonomous silencing of an endogenous target gene. 遗传学报:英文版[J]. 2019, 46(3): 149-153, http://lib.cqvip.com/Qikan/Article/Detail?id=7001975542.
[15] Gao, Feng, Zhang, BoSen, Zhao, JianHua, Huang, JiaFeng, Jia, PeiSong, Wang, Sheng, Zhang, Jie, Zhou, JianMin, Guo, HuiShan. Deacetylation of chitin oligomers increases virulence in soil-borne fungal pathogens. NATURE PLANTS[J]. 2019, 5(11): 1167-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000496526100016.
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[18] Jun Qin, Kailun Wang, Lifan Sun, Haiying Xing, Sheng Wang, Lin Li, She Chen, HuiShan Guo, Jie Zhang. The plant-specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity. ELIFE[J]. 2018, 7: https://doaj.org/article/86617a8fd0bb4a869ec818580d4111aa.
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[45] Zhang, Zhonghui, Chen, Hao, Huang, Xiahe, Xia, Ran, Zhao, Qingzhen, Lai, Jianbin, Teng, Kunling, Li, Yin, Liang, Liming, Du, Quansheng, Zhou, Xueping, Guo, Huishan, Xie, Qi. BSCTV C2 Attenuates the Degradation of SAMDC1 to Suppress DNA Methylation-Mediated Gene Silencing in Arabidopsis. PLANT CELL[J]. 2011, 23(1): 273-288, http://124.16.144.217/handle/311023/1746.
[46] Hui-Shan Guo. RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in N. benthamiana. The Plant Cell. 2010, [47] Wang, Weiquan, Wu, Yaorong, Li, Yin, Xie, Jiaying, Zhang, Zhonghui, Deng, Zhiyong, Zhang, Yiyue, Yang, Cuiping, Lai, Jianbin, Zhang, Huawei, Bao, Hongyan, Tang, Sanyuan, Yang, Chengwei, Gao, Peng, Xia, Guixian, Guo, Huishan, Xie, Qi. A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes. PLANT MOLECULAR BIOLOGY[J]. 2010, 72(1-2): 91-99, http://124.16.144.217/handle/311023/2640.
[48] Hui-Shan Guo. An efficient system to detect protein ubiquitination by argoinfiltration in Nicotiana benthamiana. The Plant Journal. 2010, [49] Gao, Feng, Zhou, BangJun, Li, GuoYing, Jia, PeiSong, Li, Hui, Zhao, YunLong, Zhao, Pan, Xia, GuiXian, Guo, HuiShan. A Glutamic Acid-Rich Protein Identified in Verticillium dahliae from an Insertional Mutagenesis Affects Microsclerotial Formation and Pathogenicity. PLOS ONE[J]. 2010, 5(12): https://doaj.org/article/df97279d9874434abfd0819bdb203299.
[50] Li, Yan, Liu, Xiuying, Huang, Li, Guo, Huishan, Wang, XiuJie. Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea. JOURNAL OF GENETICS AND GENOMICS[J]. 2010, 37(8): 493-503, http://lib.cqvip.com/Qikan/Article/Detail?id=34932138.
[51] Teng, Kunling, Chen, Hao, Lai, Jianbin, Zhang, Zhonghui, Fang, Yuanyuan, Xia, Ran, Zhou, Xueping, Guo, Huishan, Xie, Qi. Involvement of C4 Protein of Beet Severe Curly Top Virus (Family Geminiviridae) in Virus Movement. PLOSONE[J]. 2010, 5(6): http://124.16.144.217/handle/311023/2371.
[52] Hui-Shan Guo. Up-regulation of LSB1/GDU3 impacts geminivirus infection by activating the salicylic acid pathway. The Plant Journal. 2010, [53] Lai, Jianbin, Chen, Hao, Teng, Kunling, Zhao, Qingzhen, Zhang, Zhonghui, Li, Yin, Liang, Liming, Xia, Ran, Wu, Yaorong, Guo, Huishan, Xie, Qi. RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle. PLANT JOURNAL[J]. 2009, 57(5): 905-917, http://124.16.144.217/handle/311023/1712.
[54] Zheng, Nuoyan, Xia, Ran, Yang, Cuiping, Yin, Bojiao, Li, Yin, Duan, Chengguo, Liang, Liming, Guo, Huishan, Xie, Qi. Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice. VACCINE[J]. 2009, 27(36): 5001-5007, http://dx.doi.org/10.1016/j.vaccine.2009.05.073.
[55] He, XiangFeng, Fang, YuanYuan, Feng, Lei, Guo, HuiShan. Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR-NBS-LRR class R gene-derived novel miRNA in Brassica. FEBS LETTERS[J]. 2008, 582(16): 2445-2452, http://dx.doi.org/10.1016/j.febslet.2008.06.011.
[56] Duan ChengGuo, Wang ChunHan, Guo HuiShan. Delayed resistance to Cucumber mosaic virus mediated by 3'UTR-derived hairpin RNA. CHINESE SCIENCE BULLETIN[J]. 2008, 53(21): 3301-3310, http://lib.cqvip.com/Qikan/Article/Detail?id=28667998.
[57] 方荣祥. Artificial MicroRNAs Highly Accessible to Targets Confer Efficient Virus Resistance in Plants. JOURNAL OF VIROLOGY[J]. 2008, 82(22): 11084-11095, http://dx.doi.org/10.1128/JVI.01377-08.
[58] Hui-Shan Guo. SDIR1 Is a RING Finger E3 Ligase That Positively Regulates Stress-Reponsive Abscicic Acid Signaling in Arabidopsis. The Plant Cell. 2007, [59] Du, QuanSheng, Duan, ChengGuo, Zhang, ZhongHui, Fang, YuanYuan, Fang, RongXiang, Xie, Qi, Guo, HuiShan. DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. JOURNAL OF VIROLOGY[J]. 2007, 81(17): 9142-9151, http://dx.doi.org/10.1128/JVI.02885-06.
[60] 段成国, 王春晗, 郭惠珊. microRNA对植物生长发育和病毒侵染的调控. 科学通报[J]. 2006, 51(4): 369-377, https://d.wanfangdata.com.cn/periodical/kxtb200604001.
[61] Duan, CG, Wang, CH, Guo, HS. Regulation of microRNA on plant development and viral infection. CHINESE SCIENCE BULLETIN[J]. 2006, 51(3): 269-278, http://dx.doi.org/10.1007/s11434-006-0269-3.
[62] Xie, Qi, Guo, HuiShan. Systemic antiviral silencing in plants. VIRUS RESEARCHnull. 2006, 118(1-2): 1-6, http://dx.doi.org/10.1016/j.virusres.2005.11.012.
[63] Wang, MB, Metzlaff, M. RNA silencing and antiviral defense in plants. CURRENT OPINION IN PLANT BIOLOGYnull. 2005, 8(2): 216-222, http://dx.doi.org/10.1016/j.pbi.2005.01.006.
[64] Guo, HS, Xie, Q, Fei, JF, Chua, NH. MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development. PLANT CELL[J]. 2005, 17(5): 1376-1386, http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=15829603&retmode=xml.
[65] Wang Jing-Jing, Guo Hui-Shan. Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by MicroRNA847 Upregulates Auxin Signaling to Modulate Cell Proliferation and Lateral Organ Growth in Arabidopsis. THE PLANT CELL. 27(3): 574-590, https://www.webofscience.com/wos/woscc/full-record/WOS:000354640200010.

（1） 小分子RNA介导的基因表达调控, Regulation of Gene Expression by Small RNAs, 科学出版社, 2012-01, 第 1 作者
（2） 黄瓜花叶病毒卫星RNA小RNA的克隆和概述, Cloning and Profi ling of Small RNAs from Cucumber Mosaic Virus Satellite RNA, Springer Protocol, Humana Press Inc, 2014-11, 第 5 作者
（3） 植物转基因诱导基因沉默, Transgene-Induced Gene Silencing in Plants, Springer Science+Business Media New York, 2015-01, 第 2 作者

   

（ 1 ） RNA沉默和植物抗病分子机制, 主持, 国家级, 2005-09--2009-09
（ 2 ） 转录后RNA调控机制及其抗病应用研究, 主持, 国家级, 2006-01--2009-12
（ 3 ） 病毒功能性小RNA的筛选及靶标基因验证体系研究, 主持, 国家级, 2006-12--2008-12
（ 4 ） 农作物重大病害成灾的机理与控制的基础研究, 参与, 国家级, 2006-09--2010-09
（ 5 ） 重要微生物病原的致病机理及植物抗病性的分子机理, 参与, 国家级, 2006-09--2009-12
（ 6 ） 表观调控因子在植物内源基因沉默的系统传递中的作用机理, 主持, 国家级, 2009-01--2011-12
（ 7 ） miRNA介导的抗棉花黄萎病新技术体系的建立, 主持, 国家级, 2009-01--2011-12
（ 8 ） 农作物主要病害发生的分子机理及防控策略, 主持, 国家级, 2011-01--2014-12
（ 9 ） 病毒基因沉默抑制子干扰寄主表观遗传调控的新机制, 主持, 国家级, 2011-01--2014-12
（ 10 ） 植物免疫机制与作物抗病分子设计的重大基础理论, 参与, 国家级, 2011-01--2015-12
（ 11 ） 农作物重要病毒病昆虫传播与致害的生物学基础, 参与, 国家级, 2014-01--2018-12
（ 12 ） 棉花黄萎病重要致病基因的克隆及功能验证, 主持, 国家级, 2014-01--2016-12
（ 13 ） 作物病虫害的导向性防控-生物间信息流与行为操纵, 主持, 部委级, 2014-07--2019-06
（ 14 ） 表观调控因子对沉默信号在细胞间的移动和感知的作用研 究, 主持, 国家级, 2013-01--2014-12
（ 15 ） 表观遗传因子对沉默信号在植物长距离传递的作用研究, 主持, 国家级, 2016-01--2016-12
（ 16 ） 寄主依赖的土壤微生物组和棉花-黄萎病菌跨界信息流, 主持, 市地级, 2016-09--2018-08
（ 17 ） 棉花－黄萎病菌跨界RNAi及相关效应蛋白的调控机理和抗病应用研究, 主持, 国家级, 2018-01--2022-12
（ 18 ） 靶向性作物病虫防控体系的创建与示范, 主持, 部委级, 2019-01--2020-06

（1）A Brassica miRNA Regulates Plant Growth and Immunity through Distinct Modes of Action   第一届“植物病毒前沿论坛”   2019-12-04
（2）大丽轮枝菌抵御植物免疫的策略   第三届作物黄萎病研究专题学术研讨会   2019-07-30
（3）A novel strategy of preventing chitin-triggered plant immunity by fungal pathogen Verticillium dahliae   2019-07-13
（4）Chromatin remodeling contributes to virulence of the pathogenic fungus Verticillium dahliae   2019年国际植物免疫学研讨会   2019-06-24
（5）Learning from the interaction between cotton and Verticillium dahliae   2019-05-30
（6）Cotton Verticillium disease and trans-kingdom antifungal RNAi   黄萎病研究专题学术研讨会   2018-11-11
（7）Cotton Verticillium disease and trans-kingdom antifungal RNAi   2018微生物学国际前沿研讨会   2018-11-09
（8）Cotton-Verticillium interaction and trans-kingdom RNAi for combating cotton vascular wilt disease    第三届“分子植物”国际研讨会：植物-环境互作会议   2018-06-12
（9）RNA沉默-植物抗病毒研究进展   中国植物病毒学发展方向战略研讨会（预备会议）   2018-03-27
（10）Learning from the interaction between cotton and Verticillium dahliae   第五届植物-生物互作国际会议   2017-08-17
（11）棉花黄萎病致病机理和植物-真菌跨界抗病RNAi   植物与黄萎病互作与防控研讨会   2017-04-21
（12）大丽轮枝菌致病机理和棉花抗黄萎病应用研究   2016年全国植物生物学大会   2016-10-09
（13）Cmv2b-AGO interaction is required for the suppression of RDR-dependent antivival silencing in Arabidopsis   中国植物病理学会病毒与生物技术2016年学术年会   2016-08-18
（14）Application of RNAi in resistance to wilt disease pathogen in cotton   2016-05-13
（15）Host-induced gene silencing (HIGS) of target gene in fungal cells confers resistance to cotton wilt disease pathogen Verticillium dahiiae   中国植物生理与植物分子生物学学会 植物生物学女科学家分会第三界学术交流会   2016-04-01
（16）miRNA-related regulation of Auxin signaling pathway   辰山学术论坛   guo huishan    2015-08-04
（17）Genome-wide analysis of the effect of 2b protein on DNA methylation   guo huishan    2015-08-01
（18）Functional study of novel nonconserved plant miRNAs   2014年全国植物病毒学术年会   guo huishan    2014-10-06
（19）miRNA directs mRNA cleavage of IAA28 to upregulate auxin signals for Arabidopsis ateral organs development   中国植物生理与植物分子生物学学会第十一次会员代表大会暨全国学术年会   guo huishan    2014-08-05

已指导学生

应晓宝  博士研究生  071007-遗传学  

段成国  博士研究生  071007-遗传学  

杜全生  博士研究生  071007-遗传学  

何祥凤  博士研究生  071007-遗传学  

周邦军  博士研究生  071007-遗传学  

李焱  硕士研究生  071007-遗传学  

冯镭  博士研究生  071007-遗传学  

朱慧  博士研究生  071007-遗传学  

徐宁  博士研究生  071007-遗传学  

房媛媛  博士研究生  071007-遗传学  

赵盼  博士研究生  071007-遗传学  

赵建华  博士研究生  071007-遗传学  

张涛  博士研究生  071007-遗传学  

倪浩  博士研究生  071007-遗传学  

赵云龙  博士研究生  071007-遗传学  

汪晶晶  博士研究生  071007-遗传学  

周婷婷  博士研究生  071007-遗传学  

陈中祺  博士研究生  071007-遗传学  

刘晓兰  博士研究生  071007-遗传学  

杜旋  硕士研究生  071007-遗传学  

王胜  博士研究生  071007-遗传学  

马晓薇  硕士研究生  071007-遗传学  

现指导学生

李洁  博士研究生  071007-遗传学  

崔辰  博士研究生  071007-遗传学  

武雪明  博士研究生  071007-遗传学  

尚靖芸  硕士研究生  071007-遗传学  

刘传慧  硕士研究生  071007-遗传学  

吴华伟  博士研究生  071007-遗传学  

刘清艳  硕士研究生  071007-遗传学  

张博森  博士研究生  071007-遗传学  

赵建华 副研究员 zhao_jian_hua@hotmail.com

金　芸 助理研究员 jiny@im.ac.cn

张 涛 助理研究员 zhtlyy@163.com

房媛媛 高级工程师 fangyy@im.ac.cn