Mammalian genetic circuit; gene therapy, vaccine design, predictive models, synthetic biology, anti-tuberculosis drug

EDUCATION

B.S. Physics, Wuhan University, 1999.9-2003.7.

Ph.D. Physics, Peking University, 2003.9-2009.1.

PROFESSIONAL EMPLOYMENT

2019.9-now: Professor, Shenzhen Institute of Advanced Technology, Chinese Academy of Science.

2013.3-2019.6: Professor, Institute of Microbiology, Chinese Academy of Science.

2011.8-2013.1: Postdoctoral associate, Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology.

2009.8-2011.8: Postdoctoral Scholar, Department of Pharmaceutical Chemistry, University of California, San Francisco.

2003.9-2009.7: Research Assistant, Center for Theoretical Biology, School of Physics, Peking University

Best Poster and presenter Award, Synthetic Biology Engineering Research Center (SynBERC) conference, 2011

Grand Prize, iGEM competition, Mentor and Project Designer of PKU Team, 2007

Zhong Shengbiao Scholarship, Peking University, 2007

37. Guan, Y., Chen, X., Shao, B., Ji, X., Xiang, Y., Jiang, G., ... & Lou, C.* (2022). Mitigating Host Burden of Genetic Circuits by Engineering Autonegatively Regulated Parts and Improving Functional Prediction. ACS Synthetic Biology, 11(7), 2361-2371.

36. Kato, Y.*, & Lou, C.* (2022). Synthetic Genetic Elements, Devices, and Systems. Life, 12(7), 945.

35. Zhao, X., Wei, W., Zong, Y., Bai, C., Guo, X., Zhu, H.*, & Lou, C*. (2022). Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces. Synthetic and Systems Biotechnology.

34. Zhao, X.*, Zong, Y., Wei, W., & Lou, C*. (2022). Multiplexed Promoter Engineering for Improving Thaxtomin A Production in Heterologous Streptomyces Hosts. Life, 12(5), 689.

33. Sun, Z., Wei, W., Zhang, M., Shi, W., Zong, Y., Chen, Y., ... & Lou, C*. (2022). Synthetic robust perfect adaptation achieved by negative feedback coupling with linear weak positive feedback. Nucleic Acids Research, 50(4), 2377-2386.

32. Du, P. *, Lou, C., Zhao, X., Wang, Q., Ji, X., & Wei, W. (2021). CRISPR-Based Genetic Switches and Other Complex Circuits: Research and Application. Life, 11(11), 1255.

31. Zhang, Y., Wang, Y., Xu, L., Lou, C., Ouyang, Q. *, & Qian, L*. (2021). Paired dCas9 design as a nucleic acid detection platform for pathogenic strains. Methods.

30. Chen, Z., Sun, J., Guan, Y., Li, M., Lou, C. *, & Wu, B*. (2021). Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli. Synthetic and systems biotechnology, 6(4), 326-334.

29. Ji, X., Zhao, H., Zhu, H., Zhu, K., Tang, S. Y.*, & Lou, C.* (2020). CRISPRi/dCpf1-mediated dynamic metabolic switch to enhance butenoic acid production in Escherichia coli. Applied microbiology and biotechnology, 104, 5385–5393.

28. Du, P., Zhao, H., Zhang, H., Wang, R., Huang, J., Tian, Y., ... Tao, Y*& Lou, C*. (2020). De novo design of an intercellular signaling toolbox for multi-channel cell–cell communication and biological computation. Nature communications, 11(1), 1-11.

27. Miao, C., Zhao, H., Qian, L.*, & Lou, C.* (2019). Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells. Synthetic and Systems Biotechnology, 4(1), 1-9.

26. Zheng, Y., Meng, F., Zhu, Z., Wei, W., Sun, Z., Chen, J., Lou, C*. & Chen, G. Q*. (2019). A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. Nucleic acids research, 47(21), e137-e137.

25. Hao, T., Xie, Z., Wang, M., Liu, L., Zhang, Y., Wang, W., ... & Gao, S. (2019). An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters. Nature communications, 10(1), 1-13.

24. 孟凡康, & 娄春波. (2018). 人工遗传改造生命体的防逃逸技术研究进展. 有机化学, 1-12.

23. Li, T., Dong, Y., Zhang, X., Ji, X., Luo, C., Lou, C.,  & Ouyang, Q. (2018). Engineering of a genetic circuit with regulatable multistability. Integrative Biology, 10(8), 474-482.

22. Hou, J., Zeng, W., Zong, Y., Chen, Z., Miao, C., Wang, B., & Lou, C*. (2018). Engineering the Ultrasensitive Transcription Factors by Fusing a Modular Oligomerization Domain. ACS synthetic biology, 7(5), 1188-1194.

21. Tang, W., Guo, Z., Cao, Z., Wang, M., Li, P., Meng, X., ..., Lou, & C. Chen, Y*. (2018). d-Sedoheptulose-7-phosphate is a common precursor for the heptoses of septacidin and hygromycin B. Proceedings of the National Academy of Sciences, 115(11), 2818-2823.

20. Liu Q, Schumacher J, Wan X, Lou C, Wang B*. Orthogonality and Burdens of Heterologous AND Gate Gene Circuits in E. coli. ACS Synthetic Biology. 2018 Jan 5

19. Tang Q, Lou C*, Liu SJ*. Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit. Journal of biological engineering, 2017, 11(1): 32.

18. Zeng W, Du P, Lou Q, Wu L, Zhang HM, Lou C, Wang H, Ouyang Q*. Rational Design of an Ultrasensitive Quorum-Sensing Switch. (2017). ACS Synthetic Biology. 2017 6(8):1445-1452

17. 季翔宇, 赵会伟, 娄春波*. 人工基因元件的生理学研究进展. 生物工程学报, 2017, 33(3): 393-403.

16. Zong, Y., Zhang, H. M., Lyu, C., Ji, X., Hou, J., Guo, X., Ouyang Q*, & Lou, C*. (2017). Insulated transcriptional elements enable precise design of genetic circuits. Nature communications, 8(1), 52.

15. Zhang Y., Qian L., Wei W., …, Ouyang Q. *, & Lou C*. (2016). Paired design of dCas9 as a systematic platform for the detection of featured nucleic acid sequences in pathogenic strains. ACS synthetic biology. 6(2), 211-216

14. Li, T., Ye, J., Shen, R., Zong, Y., Zhao, X., Lou, C. *, & Chen, G. Q. * (2016). Semi-rational approach for ultra-high poly (3-hydroxybutyrate) accumulation in Escherichia coli by combining one-step library construction and high-throughput screening. ACS synthetic biology. 5(11), 1308-1317

13. Fang, M., Wang, T., Zhang, C., Bai, J., Zheng, X., Zhao, X., Lou, C. & Xing, X. H. (2016). Intermediate-sensor assisted push–pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli. Metabolic engineering, 33, 41-51.

12. Zhang, H. M., Chen, S., Shi, H., Ji, W., Zong, Y., Ouyang, Q. *, & Lou, C*. (2015). Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly. ACS synthetic biology, 5(3), 269-273.

11. Zhang S, Zhao X, Tao Y* and Lou C*. (2015). A novel approach for metabolic pathway optimization: oligo-liker mediated assembly (OLMA) method. Journal of Biological Engineering, 9(1):1-10

10. Pei Du, Chensi Miao, Qiuli Lou, Zefeng Wang*, and Chunbo Lou* (2015). Engineering translational activators with CRISPR-Cas system. ACS synthetic biology. 5(1), 74-80

9. Chaoxian Bai#, Yang Zhang#, Xuejin Zhao#, Yiling Hu, Sihai Xiang, Jin Miao, Lixin Zhang* and Chunbo Lou* (2015)  Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces. PNAS, 112(39): 12181-12186.

8. Wenjun Jiang, Xuejin Zhao, Tslil Gabrieli, Chunbo Lou*, Yuval Ebenstein*, and Ting Zhu *. (2015). Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters. Nature communs,6.

7. Tingting Li^#, Teng Li^#, Weiyue Ji, Qiuyue Wang, Haoqian Zhang, Guo-Qiang Chen, Chunbo Lou* and Qi Ouyang*. (2015) Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp. Biotechnol. J.  DOI: 10.1002/biot.201400828

Zhang, B., Zhou, N., Liu, Y. M., Liu, C., Lou, C. B., Jiang, C. Y., & Liu, S. J. (2015). Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum. Microbial cell factories, 14(1), 71.

Before 2013

6. Lou CB, Stanton B, Chen YJ Munsky B, Voigt CA. 2012. Ribozyme-based “insulator parts” buffer synthetic circuits from genetic context. Nature Biotechnol. 30(11), 1137-1142. (IF=43.1)

5. Moon TS, Lou CB, Stanton B, Tamsir A, Voigt CA. 2012. Genetic programs constructed from layered logic gates in single cells. Nature. 491(7423), 249-253. (IF=38.1)

4. Lou CB, Liu XL, et al. 2010. Synthesizing a novel genetic sequential logic circuit. Mol. Syst. Biol. 6:350.  (IF=10.6)

3. Lou CB, Li ZY and Ouyang Q. 2008. A molecular model for persister in E. coli. J. Theor. Biol. 255: 205-209.  (IF=2.05)

2. Yang XJ, Liu XL, Lou CB, Chen JG and Ouyang Q. 2008. A case study of the dynamics of In vitro DNA evolution under constant selection pressure. J. Mol. Evol.  (IF=1.85)

1. Lou CB, Yang XJ, Liu XL, He B and Ouyang Q. 2007. A quantitative study of lambda-phage Switch and its components. Biophys J. 92: 2685-2693.  (IF=3.63)

1. Synthetic Biology Tools, Chunbo Lou, Tae Seok Moon, Virgil Rhodius, Brynne Stanton, Alvin Tamsir, Karsten Temme and Christopher Voigt, U.S. Patent Application No. 13/489,205.：PCT/US2012/040939; WO 2012/170436.A1

2. Methods and Kits for Fragmenting DNA, Ting F. Zhu, Yuval Ebenstein, Chunbo Lou, Wenjun Jiang, Xuejin Zhao, Tslil Gabrieli, U.S. Patent Application No. 62/156,417;  PCT/IL2016/0503394.; WO 2016/178207.A1

3. Novel Oligo-linker-mediated DNA Assembly Method and Applications Thereof.  Chunb Lou, Yong Tao, Xuejin Zhao, Shasha Zhang, Lihua Zhang, Chunhua Zhai, Zhenyu Liu, U.S. Patent Application US2016/0340670. A1

4. 基于CRISPR/Cas9和酿酒酵母细胞内源的同源重组的分子克隆方法，娄春波，赵学金， 发明专利CN 105624146,专利号ZL 2015 1 0282184.X

5. 构建生产或高产目的基因产物重组菌的方法及构建的重组菌与应用, 张立新;胡逸灵;娄春波;白超弦;苗靳;向四海;黄佩;, 发明专利CN105176899A

核酸的检测方法,  娄春波;欧阳颀;张益豪;魏伟佳; 发明专利CN105177110A

6.  阻遏蛋白、调控元件组和基因表达调控系统及其构建方法  娄春波;侯君然; 发明专利CN107344962A

7. 用于处理重金属污染的微生物细胞和蛋白、相应的处理方法和试剂盒 娄春波;杜沛;魏伟佳;王瑞沙;姜浩;张浩千;  发明专利CN107916243A

8. 一种可编程的多位点特异性的转录抑制系统及其应用 苗晨思;赵会伟;关莹;陈泽华;娄春波; 发明专利CN107312790A

已指导学生

宗夜晴  硕士研究生  071010-生物化学与分子生物学  

张洋  博士研究生  071010-生物化学与分子生物学  

侯君然  硕士研究生  085238-生物工程  

郭铦  硕士研究生  085238-生物工程  

王瑞沙  硕士研究生  085238-生物工程  

宗夜晴  博士研究生  071010-生物化学与分子生物学  

现指导学生

冯禹歌  博士研究生  071010-生物化学与分子生物学  

马子涵  硕士研究生  086000-生物与医药  

郑道翔  硕士研究生  086000-生物与医药