DNA-蛋白质相互作用；基于功能性核酸分析

   

070302-分析化学
077601-环境科学

DNA-蛋白质相互作用；功能性DNA的分析应用

2008-09--2012-06   中国科学院生态环境研究中心   博士研究生
2005-09--2008-06   安徽师范大学   硕士研究生
2001-09--2005-06   安徽师范大学   本科

   

2016-01~现在, 中国科学院生态环境研究中心, 副研究员
2012-07~2015-12,中国科学院生态环境研究中心, 助理研究员

   

（1） 中国分析测试协会科学技术奖, 特等奖, 省级, 2015
（2） 中国科学院优秀博士学位论文, 特等奖, 院级, 2013
（3） 中国科学院创新促进会, 特等奖, 院级, 2013
（4） 中国分析测试协会科学技术奖, 一等奖, 省级, 2013
（5） 中国科学院院长特别奖, 特等奖, 院级, 2012
（6） 中国科学院保洁优秀研究生奖学金, 一等奖, 院级, 2011

[1] 汪海林, 章大鹏, 凌晓婷. 双色激光交替激发单分子成像检测装置、使用方法和应用. 202310604676.0, 2023-05-26.
[2] 汪海林, 余方志, 章大鹏. 毛细管电泳-连续波长双光子荧光偏振检测装置. CN: CN108169192A, 2018-06-15.
[3] 汪海林, 赵超, 尹俊发, 尹瑞川, 章大鹏. 基于固定化酶微反应器的DNA测序技术. CN: CN103184278A, 2013-07-03.

   

[1] Yu, Fangzhi, Zhang, Dapeng, Zhao, Chubin, Zhao, Qiang, Jiang, Guibin, Wang, Hailin. Flanking strand separation activity of RecA nucleoprotein filaments in DNA strand exchange reactions. NUCLEIC ACIDS RESEARCH[J]. 2023, 51(5): 2270-2283, http://dx.doi.org/10.1093/nar/gkad078.
[2] Wei Li, Shuai Yang, Peng Xu, Dapeng Zhang, Ying Tong, Lu Chen, Ben Jia, Ang Li, Cheng Lian, Daoping Ru, Baolong Zhang, Mengxing Liu, Cancan Chen, Weihui Fu, Songhua Yuan, Chenjian Gu, Lu Wang, Wenxuan Li, Ying Liang, Zhicong Yang, Xiaoguang Ren, Shaoxuan Wang, Xiaoyan Zhang, Yuanlin Song, Youhua Xie, Hongzhou Lu, Jianqing Xu, Hailin Wang, Wenqiang Yu. SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network. EBIOMEDICINE[J]. 2022, 76: 103861-103861, [3] Hao, Jinghua, Cao, Dong, Zhao, Qiang, Zhang, Dapeng, Wang, Hailin. Intramolecular Folding of PolyT Oligonucleotides Induced by Cooperative Binding of Silver(I) Ions. MOLECULES[J]. 2022, 27(22): http://dx.doi.org/10.3390/molecules27227842.
[4] Liang, Ying, Lu, Qi, Li, Wei, Zhang, Dapeng, Zhang, Fanglin, Zou, Qingping, Chen, Lu, Tong, Ying, Liu, Mengxing, Wang, Shaoxuan, Li, Wenxuan, Ren, Xiaoguang, Xu, Peng, Yang, Zhicong, Dong, Shihua, Zhang, Baolong, Huang, Yanni, Li, Daqiang, Wang, Hailin, Yu, Wenqiang. Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. NUCLEIC ACIDS RESEARCH[J]. 2021, 49(15): 8556-8572, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000697383500017.
[5] Yuan, Zheng, Yu, Fangzhi, Zhang, Dapeng, Wang, Hailin. Profiling of the assembly of RecA nucleofilaments implies a potential target for environmental factors to disturb DNA repair. JOURNAL OF ENVIRONMENTAL SCIENCES[J]. 2021, 102(4): 283-290, http://dx.doi.org/10.1016/j.jes.2020.09.022.
[6] Yuan, Zheng, Zhang, Dapeng, Yu, Fangzhi, Ma, Yangde, Liu, Yan, Li, Xiangjun, Wang, Hailin. Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA. CHEMICAL SCIENCE[J]. 2021, 12(6): 2039-2049, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179319/.
[7] 余方志, 章大鹏, 袁征, 赵强, 汪海林. 亲和毛细管电泳技术在蛋白质-DNA相互作用研究中的应用. 色谱[J]. 2020, 38(10): 1133-1142, http://lib.cqvip.com/Qikan/Article/Detail?id=7102843568.
[8] Yu, Fangzhi, Yuan, Zheng, Zhang, Dapeng, Liu, Yan, Zhao, Qiang, Wang, Hailin. High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis. CHEMICAL COMMUNICATIONS[J]. 2020, 56(54): 7403-7406, https://www.webofscience.com/wos/woscc/full-record/WOS:000545820900031.
[9] Yuan, Zheng, Xu, Peng, Yu, Fangzhi, Zhang, Dapeng, Zhao, Qiang, Yu, Wenqiang, Wang, Hailin. An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA. ANALYTICAL METHODS[J]. 2020, 12(48): 5833-5838, https://www.webofscience.com/wos/woscc/full-record/WOS:000603082600006.
[10] Yu, Fangzhi, Zhao, Qiang, Zhang, Dapeng, Yuan, Zheng, Wang, Hailin. Affinity Interactions by Capillary Electrophoresis: Binding, Separation, and Detection. ANALYTICAL CHEMISTRY[J]. 2019, 91(1): 372-387, http://dx.doi.org/10.1021/acs.analchem.8b04741.
[11] Zhang, Dapeng, Wang, Hailin. Fluorescence Anisotropy Reduction of An Allosteric G-Rich Oligonucleotide for Specific Silver Ion and Cysteine Detection Based on the G-Ag+-G Base Pair. ANALYTICAL CHEMISTRY[J]. 2019, 91(22): 14538-14544, https://www.webofscience.com/wos/woscc/full-record/WOS:000498280100051.
[12] Xu, Tian, Yin, Junfa, Chen, Shaokun, Zhang, Dapeng, Wang, Hailin. Elevated 8-oxo-7,8-dihydro-2 '-deoxyguanosine in genome of T24 bladder cancer cells induced by halobenzoquinones. JOURNAL OF ENVIRONMENTAL SCIENCES[J]. 2018, 63: 133-139, http://dx.doi.org/10.1016/j.jes.2017.05.024.
[13] Zhao, Bailin, Zhang, Dapeng, Li, Chengmin, Yuan, Zheng, Yu, Fangzhi, Zhong, Shangwei, Jiang, Guibin, Yang, YunGui, Le, X Chris, Weinfeld, Michael, Zhu, Ping, Wang, Hailin. ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination. CELL DISCOVERY[J]. 2017, 3: 16053-, http://dx.doi.org/10.1038/celldisc.2016.53.
[14] Bai, Yunlong, Li, Yapiao, Zhang, Dapeng, Wang, Hailin, Zhao, Qiang. Enhancing the Affinity of Anti-Human alpha-Thrombin 15-mer DNA Aptamer and Anti-Immunoglobulin E Aptamer by PolyT Extension. ANALYTICAL CHEMISTRY[J]. 2017, 89(17): 9467-9473, http://ir.rcees.ac.cn/handle/311016/39306.
[15] Zhang, Guoqiang, Huang, Hua, Liu, Di, Cheng, Ying, Liu, Xiaoling, Zhang, Wenxin, Yin, Ruichuan, Zhang, Dapeng, Zhang, Peng, Liu, Jianzhao, Li, Chaoyi, Liu, Baodong, Luo, Yuewan, Zhu, Yuanxiang, Zhang, Ning, He, Shunmin, He, Chuan, Wang, Hailin, Chen, Dahua. N-6-Methyladenine DNA Modification in Drosophila. CELL[J]. 2015, 161(4): 893-906, http://dx.doi.org/10.1016/j.cell.2015.04.018.
[16] Zhong, Shangwei, Zou, Dandan, Zhao, Bailin, Zhang, Dapeng, Li, Xiangjun, Wang, Hailin. Electrophoretic behavior of DNA-methyl-CpG-binding domain protein complexes revealed by capillary electrophoreses laser-induced fluorescence. ELECTROPHORESIS[J]. 2015, 36(24): 3088-3093, https://www.webofscience.com/wos/woscc/full-record/WOS:000368028200014.
[17] Liu, Shengquan, Zhang, Dapeng, Wang, Hailin. Transient Isotachophoresis Focusing of DNA and DNA-Protein Complexes Is Essentially Enhanced by Spontaneously Dissolved Aerial Carbon Dioxide in Electrolytes. ANALYTICAL CHEMISTRY[J]. 2015, 87(1): 554-560, http://dx.doi.org/10.1021/ac504173b.
[18] Zhang, Dapeng, Fu, Rong, Zhao, Qiang, Rong, Haiqin, Wang, Hailin. Nanoparticles-Free Fluorescence Anisotropy Amplification Assay for Detection of RNA Nucleotide-Cleaving DNAzyme Activity. ANALYTICAL CHEMISTRY[J]. 2015, 87(9): 4903-4909, http://ir.rcees.ac.cn/handle/311016/32628.
[19] Zou DanDan, Wang XiaoLi, Chen ZhiLan, Zhang DaPeng, Wang HaiLin. Engineered SNAP-MBD2b proteins for specific recognition of methylated DNA. SCIENCE CHINA-CHEMISTRY[J]. 2014, 57(7): 1019-1025, http://www.irgrid.ac.cn/handle/1471x/936437.
[20] Zhang, Dapeng, Yin, Lei, Meng, Zihui, Yu, Anchi, Guo, Lianghong, Wang, Hailin. A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer. ANALYTICA CHIMICA ACTA[J]. 2014, 812(1): 161-167, http://dx.doi.org/10.1016/j.aca.2013.12.029.
[21] Zou, Dandan, Zhang, Dapeng, Liu, Shengquan, Zhao, Bailin, Wang, Hailin. Interplay of Binding Stoichiometry and Recognition Specificity for the Interaction of MBD2b Protein and Methylated DNA Revealed by Affinity Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Analysis. ANALYTICAL CHEMISTRY[J]. 2014, 86(3): 1775-1782, http://www.irgrid.ac.cn/handle/1471x/936470.
[22] Yin, Ruichuan, Zhang, Dapeng, Song, Yuling, Zhu, BenZhan, Wang, Hailin. Potent DNA damage by polyhalogenated quinones and H2O2 via a metal-independent and Intercalation-enhanced oxidation mechanism. SCIENTIFIC REPORTS[J]. 2013, 3: https://www.webofscience.com/wos/woscc/full-record/WOS:000314864100002.
[23] Liu, Shengquan, Zhao, Bailin, Zhang, Dapeng, Li, Cuiping, Wang, Hailin. Imaging of Nonuniform Motion of Single DNA Molecules Reveals the Kinetics of Varying-Field Isotachophoresis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2013, 135(12): 4644-4647, https://www.webofscience.com/wos/woscc/full-record/WOS:000317032000018.
[24] Yin, Ruichuan, Mao, ShiQing, Zhao, Bailin, Chong, Zechen, Yang, Ying, Zhao, Chao, Zhang, Dapeng, Huang, Hua, Gao, Juan, Li, Zheng, Jiao, Yan, Li, Cuiping, Liu, Shengquan, Wu, Danni, Gu, Weikuan, Yang, YunGui, Xu, GuoLiang, Wang, Hailin. Ascorbic Acid Enhances Tet-Mediated 5-Methylcytosine Oxidation and Promotes DNA Demethylation in Mammals. JOURNALOFTHEAMERICANCHEMICALSOCIETY[J]. 2013, 135(28): 10396-10403, http://dx.doi.org/10.1021/ja4028346.
[25] Geng, Xia, Zhang, Dapeng, Wang, Hailin, Zhao, Qiang. Screening interaction between ochratoxin A and aptamers by fluorescence anisotropy approach. ANALYTICAL AND BIOANALYTICAL CHEMISTRY[J]. 2013, 405(8): 2443-2449, http://www.irgrid.ac.cn/handle/1471x/844440.
[26] Zhang, Dapeng, Zhao, Qiang, Zhao, Bailin, Wang, Hailin. Fluorescence Anisotropy Reduction of Allosteric Aptamer for Sensitive and Specific Protein Signaling. ANALYTICAL CHEMISTRY[J]. 2012, 84(7): 3070-3074, http://www.irgrid.ac.cn/handle/1471x/901126.
[27] Zhao, Chao, Yin, Ruichuan, Yin, Junfa, Zhang, Dapeng, Wang, Hailin. Capillary Monolithic Bioreactor of Immobilized Snake Venom Phosphodiesterase for Mass Spectrometry Based Oligodeoxynucleotide Sequencing. ANALYTICAL CHEMISTRY[J]. 2012, 84(2): 1157-1164, http://www.irgrid.ac.cn/handle/1471x/901142.
[28] Zhang, Dapeng, Shen, Hujun, Li, Guohui, Zhao, Bailin, Yu, Anchi, Zhao, Qiang, Wang, Hailin. Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism. ANALYTICAL CHEMISTRY[J]. 2012, 84(18): 8088-8094, http://www.irgrid.ac.cn/handle/1471x/901101.
[29] Zhang, Dapeng, Lu, Meiling, Wang, Hailin. Fluorescence Anisotropy Analysis for Mapping Aptamer-Protein Interaction at the Single Nucleotide Level. JOURNALOFTHEAMERICANCHEMICALSOCIETY[J]. 2011, 133(24): 9188-9191, https://www.webofscience.com/wos/woscc/full-record/WOS:000291915100015.
[30] Li, Tao, Zhang, Dapeng, Luo, Wenru, Lu, Meiling, Wang, Zhixin, Song, Yuling, Wang, Hailin. Metal Cation Mediated-Capillary Electrophoresis of Nucleic Acids. ANALYTICAL CHEMISTRY[J]. 2010, 82(2): 487-490, http://www.irgrid.ac.cn/handle/1471x/964236.
[31] 汪海林, 章大鹏, 王智鑫, 李涛, 冯峰, 王超, 高海燕. 免疫毛细管电泳-激光诱导荧光分析DNA加合物的方法学研究. 色谱[J]. 2009, 27(5): 642-647, http://lib.cqvip.com/Qikan/Article/Detail?id=31890193.

   

（ 1 ） 基于核酸适配体修饰纳米材料去除优控污染物 的研究, 主持, 国家级, 2015-01--2019-12
（ 2 ） 基于DNAzyme的多种金属离子同时分离分析新方法研究, 主持, 国家级, 2014-01--2016-12
（ 3 ） 针对蛋白质-核酸相互作用研究的单分子荧光偏振分析方法学与技术, 参与, 国家级, 2015-01--2019-12
（ 4 ） 污染物诱发表观遗传变化与调控机制, 参与, 部委级, 2014-07--2019-06
（ 5 ） 毛细管电泳-双光子荧光偏振单分子检测装置, 参与, 国家级, 2014-01--2017-12
（ 6 ） 同源重组酶Rad51与DNA动态相互作用分析方法研究, 主持, 国家级, 2018-01--2021-12
（ 7 ） 大气中黑炭细颗粒的遗传与表观遗传毒性分析与分子机制研究, 参与, 国家级, 2018-01--2021-12

（1）基于DNAzyme鸟嘌呤增强荧光偏振信号放大检测Pb2+   第九届环境化学大会   章大鹏   2017-10-19

   

复旦大学