应用核磁共振(NMR)波谱，和其他的生物物理技术（比如顺磁共振，质谱，小角度散射等），研究生物大分子复合物（protein-protein/DNA/RNA complex) 的结构和动力学。

1. 蛋白质-核酸复合物的结构解析和动力学研究的新方法；
2. 与癌症相关(Human Cancer Pathway Protein Interaction Network, HCPIN)的蛋白质结构和功能研究；
3. 基于基因组学的生物大分子结构和功能研究。

本课题组将计划每年招收硕士生和博士生各一名，欢迎来自分析化学，生物化学，分子生物学，结构生物学，信息生物学以及生物学计算相关领域的本科生报考。

070302-分析化学

分析化学和生物化学

1999-09--2005-03   中科院武汉物理与数学研究所   博士
1990-09--1994-06   华中师范大学   学士

   

[1] 李颖, 李涛, 杨运煌, 胡锐, 刘买利. 一种新型的核酸检测方法. CN: CN112080549A, 2020-12-15.

   

[1] Li, Tao, Hu, Rui, Xia, Jianbo, Xu, Zhichen, Chen, Dongjuan, Xi, Jinou, Liu, BiFeng, Zhu, Jiang, Li, Ying, Yang, Yunhuang, Liu, Maili. G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection. BIOSENSORS & BIOELECTRONICS[J]. 2021, 187: http://dx.doi.org/10.1016/j.bios.2021.113292.
[2] Hu, Yunfei, Cheng, Kai, He, Lichun, Zhang, Xu, Jiang, Bin, Jiang, Ling, Li, Conggang, Wang, Guan, Yang, Yunhuang, Liu, Maili. NMR-Based Methods for Protein Analysis. ANALYTICAL CHEMISTRY[J]. 2021, 93(4): 1866-1879, https://www.webofscience.com/wos/woscc/full-record/WOS:000618089100003.
[3] Hu, Rui, Li, Tao, Yang, Yunhuang, Tian, Yuan, Zhang, Limin, Hu, S. NMR-Based Metabolomics in Cancer Research. CANCER METABOLOMICS: METHODS AND APPLICATIONS[J]. 2021, 1280: 201-218, http://dx.doi.org/10.1007/978-3-030-51652-9_14.
[4] Rui Hu, Ying Li, Yunhuang Yang, Maili Liu. Mass spectrometry‐based strategies for single‐cell metabolomics. Mass Spectrometry Reviews[J]. 2021, [5] Heng Zhou, Xiali Yue, Zi Wang, Shuangli Li, Jiang Zhu, Yunhuang Yang, Maili Liu. Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli. Protein Expression and Purification. 2021, 180: http://dx.doi.org/10.1016/j.pep.2021.105821.
[6] Zhang, Jingfeng, Fan, JingSong, Li, Shuangli, Yang, Yunhuang, Sun, Peng, Zhu, Qinjun, Wang, Jiannan, Jiang, Bin, Yang, Daiwen, Liu, Maili. Structural basis of DNA binding to human YB-1 cold shock domain regulated by phosphorylation. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(16): 9361-9371, http://dx.doi.org/10.1093/nar/gkaa619.
[7] Zeng, Qingbin, Bie, Binglin, Guo, Qianni, Yuan, Yaping, Han, Qi, Han, Xiaocang, Chen, Mingwei, Zhang, Xu, Yang, Yunhuang, Liu, Maili, Liu, Pan, Deng, Hexiang, Zhou, Xin. Hyperpolarized Xe NMR signal advancement by metal-organic framework entrapment in aqueous solution. Proceedings of the National Academy of Sciences of the United States of America[J]. 2020, 117(30): 17558-17563, https://www.pnas.org/doi/10.1073/pnas.2004121117.
[8] Li, Ying, Li, Tao, Liu, BiFeng, Hu, Rui, Zhu, Jiang, He, Ting, Zhou, Xin, Li, Conggang, Yang, Yunhuang, Liu, Maili. CRISPR-Cas12a trans-cleaves DNA G-quadruplexes. CHEMICAL COMMUNICATIONS[J]. 2020, 56(83): 12526-12529, https://www.webofscience.com/wos/woscc/full-record/WOS:000583607100040.
[9] Li, Tao, Ou, Gaozhi, Chen, Xuliang, Li, Zheyu, Hu, Rui, Li, Ying, Yang, Yunhuang, Liu, Maili. Naked-eye based point-of-care detection of E.coli O157: H7 by a signal-amplified microfluidic aptasensor. ANALYTICA CHIMICA ACTA[J]. 2020, 1130: 20-28, http://dx.doi.org/10.1016/j.aca.2020.07.031.
[10] Li, Shuangli, Lu, Guoliang, Fang, Xiang, Ramelot, Theresa A, Kennedy, Michael A, Zhou, Xin, Gong, Peng, Zhang, Xu, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Structural insight into the length-dependent binding of ssDNA by SP_0782 from Streptococcus pneumoniae, reveals a divergence in the DNA-binding interface of PC4-like proteins. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(1): 432-444, https://www.webofscience.com/wos/woscc/full-record/WOS:000524741700037.
[11] Zhu, Wenkai, Li, Ying, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity. JOURNAL OF PHYSICAL CHEMISTRY LETTERS[J]. 2019, 10(6): 1272-1278, [12] Rui Hu, Chao Liu, Jie Xuan, Youzhi Xu, Tao Li, BiFeng Liu, Ying Li, Yunhuang Yang. 3D Hydrodynamic Flow Focusing-based Micromixer Enables High-Resolution Imaging for Studying the Early Folding Kinetics of G-Quadruplex. Sensors & Actuators: B. Chemical. 2019, 293: 312-320, http://dx.doi.org/10.1016/j.snb.2019.05.026.
[13] Hu, Rui, He, Ting, Zhang, Zhaowei, Yang, Yunhuang, Liu, Maili. Safety analysis of edible oil products via Raman spectroscopy. TALANTA[J]. 2019, 191: 324-332, http://dx.doi.org/10.1016/j.talanta.2018.08.074.
[14] Liang, Chunjie, Zhu, Jiang, Ramelot, Theresa A, Kennedy, Michael A, Yue, Xiali, Li, Xuegang, Liu, Maili, He, Ting, Yang, Yunhuang. Solution NMR structure of CHU_1110 from Cytophaga hutchinsonii, an AHSA1 protein potentially involved in metal ion stress response. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2019, 87(1): 91-95, [15] Wang, Zi, Nie, Yao, Zhang, Kunxiao, Xu, Henghao, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Solution structure of SHIP2 SH2 domain and its interaction with a phosphotyrosine peptide from c-MET. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 656: 31-37, http://dx.doi.org/10.1016/j.abb.2018.08.012.
[16] Zhang Bin, Guo Qianni, Luo Qing, Zhang Xiaoxiao, Zeng QingBin, Zhao Longhui, Yuan Yaping, Jiang Weiping, Yang Yunhuang, Liu Maili, Ye Chaohui, Zhou Xin. An intracellular diamine oxidase triggered hyperpolarized 129Xe magnetic resonance biosensor.. Chemical communications (Cambridge, England). 2018, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=33&recid=&FileName=SJPD6FAC874E1668091EC8477308660E3A74&DbName=SJPD_06&DbCode=SJPD&yx=&pr=&URLID=&bsm=.
[17] Zhu, Jiang, Li, Shuangli, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Yang, Yunhuang. Structural insights into the impact of two holoprosencephaly-related mutations on human TGIF1 homeodomain. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 496(2): 575-581, http://dx.doi.org/10.1016/j.bbrc.2018.01.099.
[18] Li, Tao, Li, Shuangli, Liang, Chunjie, Zhu, Jiang, Liu, Maili, Yang, Yunhuang. Chemical shift assignments of RHE_RS02845, a NTF2-like domain-containing protein from Rhizobium etli. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2018, 12(2): 249-252, [19] Yang Yunhuang. Nanobody Technology for Mycotoxin Detection in the Field of Food Safety: Current Status and Prospects. 2018, [20] Shuangli Li, Rui Hu, Haijie Yao, Dong Long, Fan Luo, Xin Zhou, Xu Zhang, Maili Liu, Jiang Zhu, Yunhuang Yang. Characterization of the interaction interface and conformational dynamics of human TGIF1 homeodomain upon the binding of consensus DNA. BBA - Proteins and Proteomics. 2018, 1866(10): 1021-1028, http://dx.doi.org/10.1016/j.bbapap.2018.07.005.
[21] Everett, John K, Tejero, Roberto, Murthy, Sarath B K, Acton, Thomas B, Aramini, James M, Baran, Michael C, Benach, Jordi, Cort, John R, Eletsky, Alexander, Forouhar, Farhad, Guan, Rongjin, Kuzin, Alexandre P, Lee, HsiauWei, Liu, Gaohua, Mani, Rajeswari, Mao, Binchen, Mills, Jeffrey L, Montelione, Alexander F, Pederson, Kari, Powers, Robert, Ramelot, Theresa, Rossi, Paolo, Seetharaman, Jayaraman, Snyder, David, Swapna, G V T, Vorobiev, Sergey M, Wu, Yibing, Xiao, Rong, Yang, Yunhuang, Arrowsmith, Cheryl H, Hunt, John F, Kennedy, Michael A, Prestegard, James H, Szyperski, Thomas, Tong, Liang, Montelione, Gaetano T. A community resource of experimental data for NMR/X-ray crystal structure pairs. PROTEIN SCIENCEnull. 2016, 25(1): 30-45, https://www.webofscience.com/wos/woscc/full-record/WOS:000369820800005.
[22] Li Bo, Wang Xudong, Guo Yali, Iqbal Anam, Dong Yaping, Li Wu, Liu Weisheng, Qin Wenwu, Chen Shizhen, Zhou Xin, Yang Yunhuang. One-pot synthesis of polyamines improved magnetism and fluorescence Fe3O4-carbon dots hybrid NPs for dual modal imaging. One-pot synthesis of polyamines improved magnetism and fluorescence Fe3O4-carbon dots hybrid NPs for dual modal imaging[J]. 2016, http://119.78.100.160/handle/2SELUCUE/835.
[23] Li, Shuangli, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Yang, Yunhuang. Chemical shift assignments of the homodimer protein SP_0782 (7-79) from Streptococcus pneumoniae. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2016, 10(2): 341-344, https://www.webofscience.com/wos/woscc/full-record/WOS:000388287400021.
[24] Yunhuang Yang, Theresa A Ramelot, Shuisong Ni, Robert M McCarrick, Michael A Kennedy. Applications of NMR-based PRE and EPR-based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination. Methods in molecular biology (Clifton, N.J.). 2014, 1091: 215-227, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4075034/.
[25] Yang Yunhuang. Solution structure of the free Za domain of human DLM-1 (ZBP1/DAI), a Z-DNA binding domain. 2014, [26] Yang, Yunhuang, Ramelot, Theresa A, Lee, HsiauWei, Xiao, Rong, Everett, John K, Montelione, Gaetano T, Prestegard, James H, Kennedy, Michael A. Solution structure of a C-terminal fragment (175-257) of CV_0373 protein from Chromobacterium violaceum adopts a winged helix-turn-helix (wHTH) fold. JOURNAL OF BIOMOLECULAR NMR[J]. 2014, 60(2-3): 197-202, https://www.webofscience.com/wos/woscc/full-record/WOS:000344597500012.
[27] Yang, Yunhuang, Ramelot, Theresa A, Ni, Shuisong, McCarrick, Robert M, Kennedy, Michael A. Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements. JOURNAL OF BIOMOLECULAR NMR[J]. 2013, 55(1): 47-58, https://www.webofscience.com/wos/woscc/full-record/WOS:000314050700005.
[28] Yang, Yunhuang, Ramelot, Theresa A, Cort, John R, Garcia, Maite, Yee, Adelinda, Arrowsmith, Cheryl H, Kennedy, Michael A. Solution NMR Structure of Hypothetical Protein CV_2116 Encoded by a Viral Prophage Element in Chromobacterium violaceum. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2012, 13(6): 7354-7364, https://www.webofscience.com/wos/woscc/full-record/WOS:000306188700051.
[29] Yang, Yunhuang, Ramelot, Theresa A, Cort, John R, Wang, Dongyan, Ciccosanti, Colleen, Hamilton, Keith, Nair, Rajesh, Rost, Burkhard, Acton, Thomas B, Xiao, Rong, Everett, John K, Montelione, Gaetano T, Kennedy, Michael A. Solution NMR structure of photosystem II reaction center protein Psb28 from Synechocystis sp. strain PCC 6803. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2011, 79(1): 340-344, https://www.webofscience.com/wos/woscc/full-record/WOS:000285884100027.
[30] Yunhuang Yang, Theresa A Ramelot, John R Cort, Huang Wang, Colleen Ciccosanti, Mei Jiang, Haleema Janjua, Thomas B Acton, Rong Xiao, John K Everett, Gaetano T Montelione, Michael A Kennedy. Solution NMR Structure of Dsy0195 Homodimer from Desulfitobacterium hafniense: First Structure Representative of the YabP Domain Family of Proteins Involved in Spore Coat Assembly. Journal of structural and functional genomics. 2011, 12(3): 175-179, [31] Yang, Yunhuang, Ramelot, Theresa A, McCarrick, Robert M, Ni, Shuisong, Feldmann, Erik A, Cort, John R, Wang, Huang, Ciccosanti, Colleen, Jiang, Mei, Janjua, Haleema, Acton, Thomas B, Xiao, Rong, Everett, John K, Montelione, Gaetano T, Kennedy, Michael A. Combining NMR and EPR Methods for Homodimer Protein Structure Determination. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2010, 132(34): 11910-11913, https://www.webofscience.com/wos/woscc/full-record/WOS:000281296700034.
[32] Yang, Yunhuang, Hoyt, David, Wang, Jianjun. A complete NMR spectral assignment of the lipid-free mouse apolipoprotein A-I (apoAI) C-terminal truncation mutant, apoAI(1-216). BIOMOLECULAR NMR ASSIGNMENTS[J]. 2007, 1(1): 109-111, https://www.webofscience.com/wos/woscc/full-record/WOS:000258686800033.
[33] Yunhuang Yang, Guoyun Bai, Xu Zhang, Chaohui Ye, Maili Liu. 1H NMR spectroscopic evidence of interaction between ibuprofen and lipoproteins in human blood plasma. Analytical Biochemistry. 2004, 324(2): 292-297, http://dx.doi.org/10.1016/j.ab.2003.10.002.
[34] Liang Chunjie, Zhu Jiang, Hu Rui, Ramelot Theresa A., Kennedy Michael A., Liu Maili, Yang Yunhuang. Solution NMR structure of RHE_CH02687 from Rhizobium etli: a novel flavonoid-binding protein. Proteins. 85(5): 951-956, https://www.webofscience.com/wos/woscc/full-record/WOS:000399380400014.
[35] Zhu Jiang, Wang Huapu, Ramelot Theresa A., Kennedy Michael A., Hu Rui, Yue Xiali, Liu Maili, Yang Yunhuang. Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation. Proteins. 85(5): 957-962, https://www.webofscience.com/wos/woscc/full-record/WOS:000399380400015.