应用核磁共振研究蛋白质动态结构、相互作用和功能机制

   

070302-分析化学

生物大分子核磁共振

2005-09--2010-07   北京大学   博士
2001-09--2005-07   北京大学   学士

   

2019-06~现在, 中国科学院武汉精密测量科学与技术创新研究院, 研究员
2018-06~2019-06,苏州大学, 教授
2012-07~2018-06,北京大学, 副研究员
2010-07~2012-07,北京大学, 博士后

   

[1] Xu Dong, Lulu Yu, Qiang Zhang, Ju Yang, Zhou Gong, Xiaogang Niu, Hongwei Li, Xu Zhang, Maili Liu, Changwen Jin, Yunfei Hu. Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1. COMMUNICATIONS BIOLOGY[J]. 2024, 第 11 作者7(1): 1-11, http://dx.doi.org/10.1038/s42003-024-06258-7.
[2] Jie Heng, Yunfei Hu, Guillermo Pérez-Hernández, Asuka Inoue, Jiawei Zhao, Xiuyan Ma, Xiaoou Sun, Kouki Kawakami, Tatsuya Ikuta, Jienv Ding, Yujie Yang, Lujia Zhang, Sijia Peng, Xiaogang Niu, Hongwei Li, Ramon Guixà-González, Changwen Jin, Peter W. Hildebrand, Chunlai Chen, Brian K. Kobilka. Function and dynamics of the intrinsically disordered carboxyl terminus of β2 adrenergic receptor. NATURE COMMUNICATIONS[J]. 2023, 第 2 作者14(1): 1-18, http://dx.doi.org/10.1038/s41467-023-37233-1.
[3] Jun Xu, Qinggong Wang, Harald Hübner, Yunfei Hu, Xiaogang Niu, Haoqing Wang, Shoji Maeda, Asuka Inoue, Yuyong Tao, Peter Gmeiner, Yang Du, Changwen Jin, Brian K. Kobilka. Structural and dynamic insights into supra-physiological activation and allosteric modulation of a muscarinic acetylcholine receptor. NATURE COMMUNICATIONS[J]. 2023, 第 4 作者14(1): 1-16, http://dx.doi.org/10.1038/s41467-022-35726-z.
[4] Wang, Jiannan, Lin, Yaling, Yang, Ju, Zhang, Qiang, Liu, Maili, Hu, Yunfei, Dong, Xu. Solution structure of the DNA binding domain of Arabidopsis transcription factor WRKY11. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2023, 第 6 作者  通讯作者  653: 133-139, http://dx.doi.org/10.1016/j.bbrc.2023.02.072.
[5] Ruibo Zhai, Zhuoqi Wang, Zhaofei Chai, Xiaogang Niu, Conggang Li, Changwen Jin, Yunfei Hu. Distinct activation mechanisms of β-arrestin-1 revealed by 19 F NMR spectroscopy. NATURE COMMUNICATIONS[J]. 2023, 第 7 作者  通讯作者  14: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686989/.
[6] Hu, Yunfei, Cheng, Kai, He, Lichun, Zhang, Xu, Jiang, Bin, Jiang, Ling, Li, Conggang, Wang, Guan, Yang, Yunhuang, Liu, Maili. NMR-Based Methods for Protein Analysis. ANALYTICAL CHEMISTRY[J]. 2021, 第 1 作者93(4): 1866-1879, https://www.webofscience.com/wos/woscc/full-record/WOS:000618089100003.
[7] Jiannan Wang, Yaling Lin, Qinjun Zhu, Jingfeng Zhang, Maili Liu, Yunfei Hu. NMR assignments and characterization of the DNA-binding domain of Arabidopsis transcription factor WRKY11: Dedicated to Professor Xiuwen Han on the occasion of her 80th birthday. MAGNETIC RESONANCE LETTERS[J]. 2021, 第 6 作者  通讯作者  1(2): 112-120, 
[8] Xiuyan Ma, Yunfei Hu, Hossein Batebi, Jie Heng, Jun Xu, Xiangyu Liu, Xiaogang Niu, Hongwei Li, Peter W Hildebrand, Changwen Jin, Brian K Kobilka. Analysis of β 2 AR-G s and β 2 AR-G i complex formation by NMR spectroscopy. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2020, 第 2 作者117(37): 23096-23105, 
[9] Jia, Moye, Wu, Bo, Yang, Ziyu, Chen, Chunlai, Zhao, Meiping, Hou, Xianhui, Niu, Xiaogang, Jin, Changwen, Hu, Yunfei. Conformational Dynamics of the Periplasmic Chaperone SurA. BIOCHEMISTRY[J]. 2020, 第 9 作者  通讯作者  59(35): 3235-3246, https://www.webofscience.com/wos/woscc/full-record/WOS:000570010100006.
[10] Niu Xiaogang, Ma Shuaipeng, Hu Yunfei, Jin Changwen. Backbone 1H, 13C and 15N resonance assignments of the proteasome lid subunit Rpn12 from Saccharomyces cerevisiae. Biomol. NMR Assign.[J]. 2020, 第 3 作者
[11] Hu, Yunfei, Li, Conggang, He, Lichun, Jin, Changwen, Liu, Maili. Mechanisms of Chaperones as Active Assistant/Protector for Proteins: Insights from NMR Studies. CHINESE JOURNAL OF CHEMISTRY[J]. 2020, 第 1 作者  通讯作者  38(4): 406-413, http://lib.cqvip.com/Qikan/Article/Detail?id=7101969870.
[12] Xu, Jun, Hu, Yunfei, Kaindl, Jonas, Risel, Philipp, Huebner, Harald, Maeda, Shoji, Niu, Xiaogang, Li, Hongwei, Gmeiner, Peter, Jin, Changwen, Kobilka, Brian K. Conformational Complexity and Dynamics in a Muscarinic Receptor Revealed by NMR Spectroscopy. MOLECULAR CELL[J]. 2019, 第 2 作者75(1): 53-+, http://dx.doi.org/10.1016/j.molcel.2019.04.028.
[13] Yu, XingChi, Hu, Yunfei, Ding, Jienv, Li, Hongwei, Jin, Changwen. Structural basis and mechanism of the unfolding-induced activation of HdeA, a bacterial acid response chaperone. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2019, 第 2 作者  通讯作者  294(9): 3192-3206, http://dx.doi.org/10.1074/jbc.RA118.006398.
[14] Zhang Wenbo, Zhao Cong, Hu Yunfei, Jin Changwen. NMR 1H, 13C, 15N Backbone and side chain resonance assignment of the N-terminal domain of yeast proteasome lid subunit Rpn5. Biomol. NMR Assign.[J]. 2019, 第 3 作者
[15] Jia Moye, Hu Yunfei, Jin Changwen. 1H, 13C and 15N resonance assignments of the second peptidyl-prolyl isomerase domain of chaperone SurA from Escherichia coli. Biomol. NMR Assign.[J]. 2019, 第 2 作者  通讯作者  
[16] Zhang, Wenbo, Zhao, Cong, Hu, Yunfei, Jin, Changwen. Solution structure of the N-terminal domain of proteasome lid subunit Rpn5. BIOCHEMICALANDBIOPHYSICALRESEARCHCOMMUNICATIONS[J]. 2018, 第 3 作者  通讯作者  504(1): 225-230, http://dx.doi.org/10.1016/j.bbrc.2018.08.159.
[17] Wenbo Zhang, Xiaogang Niu, Jienv Ding, Yunfei Hu, Changwen Jin. Intra- and inter-protein couplings of backbone motions underlie protein thiol-disulfide exchange cascade. SCIENTIFIC REPORTS[J]. 2018, 第 4 作者  通讯作者  8(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000447591000105.
[18] Niu, Xiaogang, Ding, Jienv, Zhang, Wenbo, Li, Qianwen, Hu, Yunfei, Jin, Changwen. Residue selective N-15 CEST and CPMG experiments for studies of millisecond timescale protein dynamics. JOURNAL OF MAGNETIC RESONANCE[J]. 2018, 第 5 作者293: 47-55, https://www.webofscience.com/wos/woscc/full-record/WOS:000441226500007.
[19] Yu, XingChi, Yang, Chengfeng, Ding, Jienv, Niu, Xiaogang, Hu, Yunfei, Jin, Changwen. Characterizations of the Interactions between Escherichia coil Periplasmic Chaperone HdeA and Its Native Substrates during Acid Stress. BIOCHEMISTRY[J]. 2017, 第 5 作者  通讯作者  56(43): 5748-5757, https://www.webofscience.com/wos/woscc/full-record/WOS:000414383400006.
[20] Hu Yunfei, He Peng, Wu Yujie, Jin Changwen. 枯草芽孢杆菌双精氨酸转运系统TatAy蛋白的溶液结构. 波谱学杂志[J]. 2015, 第 1 作者32(2): 291-307, http://lib.cqvip.com/Qikan/Article/Detail?id=665102161.
[21] Hu, Yunfei, Wu, Yujie, Li, Qianwen, Zhang, Wenbo, Jin, Changwen. Solution Structure of Yeast Rpn9 INSIGHTS INTO PROTEASOME LID ASSEMBLY. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2015, 第 1 作者290(11): 6878-6889, http://dx.doi.org/10.1074/jbc.M114.626762.
[22] Ding, Jienv, Yang, Chengfeng, Niu, Xiaogang, Hu, Yunfei, Jin, Changwen. HdeB chaperone activity is coupled to its intrinsic dynamic properties. SCIENTIFIC REPORTS[J]. 2015, 第 4 作者  通讯作者  5: https://www.webofscience.com/wos/woscc/full-record/WOS:000365088400001.
[23] Ye, Qian, Fu, Wenyu, Hu, Yunfei, Jin, Changwen. Long-chain flavodoxin FldB from Escherichia coli. JOURNAL OF BIOMOLECULAR NMR[J]. 2014, 第 3 作者  通讯作者  60(4): 283-288, https://www.webofscience.com/wos/woscc/full-record/WOS:000348423800008.
[24] Zhang, Yi, Wang, Lei, Hu, Yunfei, Jin, Changwen. Solution structure of the TatB component of the twin-arginine translocation system. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES[J]. 2014, 第 3 作者  通讯作者  1838(7): 1881-1888, https://www.webofscience.com/wos/woscc/full-record/WOS:000336695300022.
[25] Ye, Qian, Hu, Yunfei, Jin, Changwen. Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy. PLOS ONE[J]. 2014, 第 2 作者  通讯作者  9(8): https://doaj.org/article/39cd72b352164ace94dfb36720694b4b.
[26] Wu, Yujie, Hu, Yunfei, Jin, Changwen. H-1, C-13 and N-15 resonance assignments of Rpn9, a regulatory subunit of 26S proteasome from Saccharomyces cerevisiae. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2014, 第 2 作者  通讯作者  8(2): 307-311, http://dx.doi.org/10.1007/s12104-013-9506-2.
[27] Zhang, Yi, Hu, Yunfei, Li, Hongwei, Jin, Changwen. Structural Basis for TatA Oligomerization: An NMR Study of Escherichia coli TatA Dimeric Structure. PLOS ONE[J]. 2014, 第 2 作者  通讯作者  9(8): http://dx.doi.org/10.1371/journal.pone.0103157.
[28] Wu, Yujie, Hu, Yunfei, Jin, Changwen. H-1, C-13 and N-15 resonance assignments of the VWA domain of Saccharomyces cerevisiae Rpn10, a regulatory subunit of 26S proteasome. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2014, 第 2 作者  通讯作者  8(2): 391-394, http://dx.doi.org/10.1007/s12104-013-9525-z.
[29] Ye, Qian, Hu, Yunfei, Jin, Changwen. H-1, C-13 and N-15 resonance assignments of the apo and holo states of flavodoxin YqcA from Escherichia coli. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2014, 第 2 作者  通讯作者  8(2): 269-273, https://www.webofscience.com/wos/woscc/full-record/WOS:000342080900011.
[30] Liu, Tingting, Liu, Zixu, Song, Chuanjun, Hu, Yunfei, Han, Zhifu, She, Ji, Fan, Fangfang, Wang, Jiawei, Jin, Changwen, Chang, Junbiao, Zhou, JianMin, Chai, Jijie. Chitin-Induced Dimerization Activates a Plant Immune Receptor. SCIENCE[J]. 2012, 第 4 作者336(6085): 1160-1164, http://dx.doi.org/10.1126/science.1218867.
[31] Hu, Yunfei, Zhang, Xinxin, Shi, Yunming, Zhou, Yanfeng, Zhang, Wei, Su, XiaoDong, Xia, Bin, Zhao, Jindong, Jin, Changwen. Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2011, 第 1 作者286(14): 12381-12388, http://dx.doi.org/10.1074/jbc.M110.201186.
[32] Peng, Heng, Hu, Yunfei, Zhou, Aiguo, Jin, Changwen, Pan, Weiqing. Solution structure of a Plasmodium falciparum AMA-1/MSP 1 chimeric protein vaccine candidate (PfCP-2.9) for malaria. MALARIA JOURNAL[J]. 2010, 第 2 作者9: http://dx.doi.org/10.1186/1475-2875-9-76.
[33] Hong, Jiang, Hu, Yunfei, Li, Congmin, Jia, Zongchao, Xia, Bin, Jin, Changwen. NMR Characterizations of the Ice Binding Surface of an Antifreeze Protein. PLOS ONE[J]. 2010, 第 2 作者5(12): https://doaj.org/article/5ef0a1efde7741deb4ee6cdaa7d5c58b.
[34] Hu, Yunfei, Zhao, Enwei, Li, Hongwei, Xia, Bin, Jin, Changwen. Solution NMR Structure of the TatA Component of the Twin-Arginine Protein Transport System from Gram-Positive Bacterium Bacillus subtilis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2010, 第 1 作者132(45): 15942-15944, https://www.webofscience.com/wos/woscc/full-record/WOS:000284202200036.
[35] Shao, Xuan, Lu, Jie, Hu, Yunfei, Xia, Bin, Jin, Changwen. Solution structure of the Escherichia cali HybE reveals a novel fold. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2009, 第 3 作者75(4): 1051-1056, https://www.webofscience.com/wos/woscc/full-record/WOS:000266133600023.
[36] 胡蕴菲, 金长文. 蛋白质溶液结构及动力学的核磁共振研究. 波谱学杂志[J]. 2009, 第 1 作者26(2): 151-172, http://lib.cqvip.com/Qikan/Article/Detail?id=30571518.
[37] Li, You, Hu, Yunfei, Fu, Wenyu, Xia, Bin, Jin, Changwen. Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2007, 第 2 作者360(4): 863-867, https://www.webofscience.com/wos/woscc/full-record/WOS:000248502700026.
[38] Wang, Fei, Yang, Fan, Hu, Yunfei, Wang, Xu, Wang, Xinhe, Jin, Changwen, Ma, Jiyan. Lipid interaction converts prion protein to a PrPSc-like proteinase K-Resistant conformation under physiological conditions. BIOCHEMISTRY[J]. 2007, 第 3 作者46(23): 7045-7053, https://www.webofscience.com/wos/woscc/full-record/WOS:000247141700036.
[39] Li, You, Hu, Yunfei, Zhang, Xinxin, Xu, Huimin, Lescop, Ewen, Xia, Bin, Jin, Changwen. Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2007, 第 2 作者282(15): 11078-11083, http://dx.doi.org/10.1074/jbc.M700970200.
[40] Hu, Yunfei, Li, You, Zhang, Xinxin, Guo, Xianrong, Xia, Bin, Jin, Changwen. Solution structures and backbone dynamics of a flavodoxin MioC from Escherichia coli in both apo- and holo-forms - Implications for cofactor binding and electron transfer. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2006, 第 1 作者281(46): 35454-35466, https://www.webofscience.com/wos/woscc/full-record/WOS:000241933700079.
[41] Hu Cuiyun, Yu Caifang, Liu Yanhua, Hou Xianhui, Liu Xiaoyun, Hu Yunfei, Jin Changwen. A Hybrid Mechanism for the Synechocystis Arsenate Reductase Revealed by Structural Snapshots during Arsenate Reduction *. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 第 6 作者290(36): 22262-22273, http://dx.doi.org/10.1074/jbc.M115.659896.

   

（ 1 ） 细胞中生物大分子结构与相互作用的谱学测量, 参与, 国家任务, 2020-01--2024-12
（ 2 ） 院BRJH青年才俊, 负责人, 中国科学院计划, 2020-01--2022-12
（ 3 ） 中国科学院率先行动引才计划, 负责人, 中国科学院计划, 2022-01--2025-12
（ 4 ） 武汉黄鹤英才项目, 负责人, 地方任务, 2021-01--2024-12
（ 5 ） 重要G蛋白偶联受体瞬态结构与功能调控机制, 负责人, 研究所自主部署, 2024-04--2025-12

（1）Structural Dynamics of β-Arrestin-1 Probed by 19F NMR   深圳医学科学院第二期学术研讨会   2023-05-07
（2）植物固有无序VQ蛋白调控WRKY转录因子机制的NMR研究   第21届全国波谱学学术会议   2021-07-10
（3）Molecular basis for proteasome lid assembly via PCI domains revealed by structures and interactions of yeast Rpn9   第五届海峡两岸磁共振研讨会   2015-01-18
（4）Structures and dynamics of bacterial flavodoxins   太平洋地区国际化学大会   2010-12-15