070302-分析化学

生物大分子核磁共振

2005-09--2010-07   北京大学   博士
2001-09--2005-07   北京大学   学士

   

2019-06~现在, 中国科学院武汉精密测量科学与技术创新研究院, 研究员
2018-06~2019-06,苏州大学, 教授
2012-07~2018-06,北京大学, 副研究员
2010-07~2012-07,北京大学, 博士后

   

[1] Hu, Yunfei, Cheng, Kai, He, Lichun, Zhang, Xu, Jiang, Bin, Jiang, Ling, Li, Conggang, Wang, Guan, Yang, Yunhuang, Liu, Maili. NMR-Based Methods for Protein Analysis. ANALYTICAL CHEMISTRY[J]. 2021, 93(4): 1866-1879, https://www.webofscience.com/wos/woscc/full-record/WOS:000618089100003.
[2] Jiannan Wang, Yaling Lin, Qinjun Zhu, Jingfeng Zhang, Maili Liu, Yunfei Hu. NMR assignments and characterization of the DNA-binding domain of Arabidopsis transcription factor WRKY11: Dedicated to Professor Xiuwen Han on the occasion of her 80th birthday. Magnetic Resonance Letters. 2021, 1(2): 112-120, [3] Xiuyan Ma, Yunfei Hu, Hossein Batebi, Jie Heng, Jun Xu, Xiangyu Liu, Xiaogang Niu, Hongwei Li, Peter W Hildebrand, Changwen Jin, Brian K Kobilka. Analysis of β 2 AR-G s and β 2 AR-G i complex formation by NMR spectroscopy. Proceedings of the National Academy of Sciences of the United States of America. 2020, 117(37): 23096-23105, [4] Jia, Moye, Wu, Bo, Yang, Ziyu, Chen, Chunlai, Zhao, Meiping, Hou, Xianhui, Niu, Xiaogang, Jin, Changwen, Hu, Yunfei. Conformational Dynamics of the Periplasmic Chaperone SurA. BIOCHEMISTRY[J]. 2020, 59(35): 3235-3246, https://www.webofscience.com/wos/woscc/full-record/WOS:000570010100006.
[5] Niu Xiaogang, Ma Shuaipeng, Hu Yunfei, Jin Changwen. Backbone 1H, 13C and 15N resonance assignments of the proteasome lid subunit Rpn12 from Saccharomyces cerevisiae. Biomol. NMR Assign.[J]. 2020, [6] Hu, Yunfei, Li, Conggang, He, Lichun, Jin, Changwen, Liu, Maili. Mechanisms of Chaperones as Active Assistant/Protector for Proteins: Insights from NMR Studies. CHINESE JOURNAL OF CHEMISTRYnull. 2020, 38(4): 406-413, http://lib.cqvip.com/Qikan/Article/Detail?id=7101969870.
[7] Xu, Jun, Hu, Yunfei, Kaindl, Jonas, Risel, Philipp, Huebner, Harald, Maeda, Shoji, Niu, Xiaogang, Li, Hongwei, Gmeiner, Peter, Jin, Changwen, Kobilka, Brian K. Conformational Complexity and Dynamics in a Muscarinic Receptor Revealed by NMR Spectroscopy. MOLECULAR CELL[J]. 2019, 75(1): 53-+, http://dx.doi.org/10.1016/j.molcel.2019.04.028.
[8] Yu, XingChi, Hu, Yunfei, Ding, Jienv, Li, Hongwei, Jin, Changwen. Structural basis and mechanism of the unfolding-induced activation of HdeA, a bacterial acid response chaperone. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2019, 294(9): 3192-3206, http://dx.doi.org/10.1074/jbc.RA118.006398.
[9] Zhang Wenbo, Zhao Cong, Hu Yunfei, Jin Changwen. NMR 1H, 13C, 15N Backbone and side chain resonance assignment of the N-terminal domain of yeast proteasome lid subunit Rpn5. Biomol. NMR Assign.[J]. 2019, [10] Jia Moye, Hu Yunfei, Jin Changwen. 1H, 13C and 15N resonance assignments of the second peptidyl-prolyl isomerase domain of chaperone SurA from Escherichia coli. Biomol. NMR Assign.[J]. 2019, [11] Zhang, Wenbo, Zhao, Cong, Hu, Yunfei, Jin, Changwen. Solution structure of the N-terminal domain of proteasome lid subunit Rpn5. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 504(1): 225-230, http://dx.doi.org/10.1016/j.bbrc.2018.08.159.
[12] Wenbo Zhang, Xiaogang Niu, Jienv Ding, Yunfei Hu, Changwen Jin. Intra- and inter-protein couplings of backbone motions underlie protein thiol-disulfide exchange cascade. SCIENTIFIC REPORTS[J]. 2018, 8(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000447591000105.
[13] Niu, Xiaogang, Ding, Jienv, Zhang, Wenbo, Li, Qianwen, Hu, Yunfei, Jin, Changwen. Residue selective N-15 CEST and CPMG experiments for studies of millisecond timescale protein dynamics. JOURNAL OF MAGNETIC RESONANCE[J]. 2018, 293: 47-55, https://www.webofscience.com/wos/woscc/full-record/WOS:000441226500007.
[14] Yu, XingChi, Yang, Chengfeng, Ding, Jienv, Niu, Xiaogang, Hu, Yunfei, Jin, Changwen. Characterizations of the Interactions between Escherichia coil Periplasmic Chaperone HdeA and Its Native Substrates during Acid Stress. BIOCHEMISTRY[J]. 2017, 56(43): 5748-5757, https://www.webofscience.com/wos/woscc/full-record/WOS:000414383400006.
[15] Hu Yunfei, He Peng, Wu Yujie, Jin Changwen. 枯草芽孢杆菌双精氨酸转运系统TatAy蛋白的溶液结构. 波谱学杂志[J]. 2015, 32(2): 291-307, http://lib.cqvip.com/Qikan/Article/Detail?id=665102161.
[16] Hu, Yunfei, Wu, Yujie, Li, Qianwen, Zhang, Wenbo, Jin, Changwen. Solution Structure of Yeast Rpn9 INSIGHTS INTO PROTEASOME LID ASSEMBLY. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2015, 290(11): 6878-6889, http://dx.doi.org/10.1074/jbc.M114.626762.
[17] Ding, Jienv, Yang, Chengfeng, Niu, Xiaogang, Hu, Yunfei, Jin, Changwen. HdeB chaperone activity is coupled to its intrinsic dynamic properties. SCIENTIFIC REPORTS[J]. 2015, 5: https://www.webofscience.com/wos/woscc/full-record/WOS:000365088400001.
[18] Ye, Qian, Fu, Wenyu, Hu, Yunfei, Jin, Changwen. Long-chain flavodoxin FldB from Escherichia coli. JOURNAL OF BIOMOLECULAR NMR[J]. 2014, 60(4): 283-288, https://www.webofscience.com/wos/woscc/full-record/WOS:000348423800008.
[19] Zhang, Yi, Wang, Lei, Hu, Yunfei, Jin, Changwen. Solution structure of the TatB component of the twin-arginine translocation system. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES[J]. 2014, 1838(7): 1881-1888, https://www.webofscience.com/wos/woscc/full-record/WOS:000336695300022.
[20] Wu, Yujie, Hu, Yunfei, Jin, Changwen. H-1, C-13 and N-15 resonance assignments of Rpn9, a regulatory subunit of 26S proteasome from Saccharomyces cerevisiae. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2014, 8(2): 307-311, https://www.webofscience.com/wos/woscc/full-record/WOS:000342080900019.
[21] Ye, Qian, Hu, Yunfei, Jin, Changwen. Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy. PLOS ONE[J]. 2014, 9(8): https://doaj.org/article/39cd72b352164ace94dfb36720694b4b.
[22] Zhang, Yi, Hu, Yunfei, Li, Hongwei, Jin, Changwen. Structural Basis for TatA Oligomerization: An NMR Study of Escherichia coli TatA Dimeric Structure. PLOS ONE[J]. 2014, 9(8): https://doaj.org/article/79b906ea652f4b75b6583799fa294503.
[23] Wu, Yujie, Hu, Yunfei, Jin, Changwen. H-1, C-13 and N-15 resonance assignments of the VWA domain of Saccharomyces cerevisiae Rpn10, a regulatory subunit of 26S proteasome. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2014, 8(2): 391-394, https://www.webofscience.com/wos/woscc/full-record/WOS:000342080900036.
[24] Ye, Qian, Hu, Yunfei, Jin, Changwen. H-1, C-13 and N-15 resonance assignments of the apo and holo states of flavodoxin YqcA from Escherichia coli. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2014, 8(2): 269-273, https://www.webofscience.com/wos/woscc/full-record/WOS:000342080900011.
[25] Liu, Tingting, Liu, Zixu, Song, Chuanjun, Hu, Yunfei, Han, Zhifu, She, Ji, Fan, Fangfang, Wang, Jiawei, Jin, Changwen, Chang, Junbiao, Zhou, JianMin, Chai, Jijie. Chitin-Induced Dimerization Activates a Plant Immune Receptor. SCIENCE[J]. 2012, 336(6085): 1160-1164, https://www.webofscience.com/wos/woscc/full-record/WOS:000304647900050.
[26] Hu, Yunfei, Zhang, Xinxin, Shi, Yunming, Zhou, Yanfeng, Zhang, Wei, Su, XiaoDong, Xia, Bin, Zhao, Jindong, Jin, Changwen. Structures of Anabaena Calcium-binding Protein CcbP INSIGHTS INTO CA(2+) SIGNALING DURING HETEROCYST DIFFERENTIATION. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2011, 286(14): 12381-12388, http://dx.doi.org/10.1074/jbc.M110.201186.
[27] Peng, Heng, Hu, Yunfei, Zhou, Aiguo, Jin, Changwen, Pan, Weiqing. Solution structure of a Plasmodium falciparum AMA-1/MSP 1 chimeric protein vaccine candidate (PfCP-2.9) for malaria. MALARIA JOURNAL[J]. 2010, 9: http://dx.doi.org/10.1186/1475-2875-9-76.
[28] Hong, Jiang, Hu, Yunfei, Li, Congmin, Jia, Zongchao, Xia, Bin, Jin, Changwen. NMR Characterizations of the Ice Binding Surface of an Antifreeze Protein. PLOS ONE[J]. 2010, 5(12): https://doaj.org/article/5ef0a1efde7741deb4ee6cdaa7d5c58b.
[29] Hu, Yunfei, Zhao, Enwei, Li, Hongwei, Xia, Bin, Jin, Changwen. Solution NMR Structure of the TatA Component of the Twin-Arginine Protein Transport System from Gram-Positive Bacterium Bacillus subtilis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2010, 132(45): 15942-15944, https://www.webofscience.com/wos/woscc/full-record/WOS:000284202200036.
[30] Shao, Xuan, Lu, Jie, Hu, Yunfei, Xia, Bin, Jin, Changwen. Solution structure of the Escherichia cali HybE reveals a novel fold. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2009, 75(4): 1051-1056, https://www.webofscience.com/wos/woscc/full-record/WOS:000266133600023.
[31] 胡蕴菲, 金长文. 蛋白质溶液结构及动力学的核磁共振研究. 波谱学杂志[J]. 2009, 26(2): 151-172, http://lib.cqvip.com/Qikan/Article/Detail?id=30571518.
[32] Li, You, Hu, Yunfei, Fu, Wenyu, Xia, Bin, Jin, Changwen. Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2007, 360(4): 863-867, https://www.webofscience.com/wos/woscc/full-record/WOS:000248502700026.
[33] Wang, Fei, Yang, Fan, Hu, Yunfei, Wang, Xu, Wang, Xinhe, Jin, Changwen, Ma, Jiyan. Lipid interaction converts prion protein to a PrPSc-like proteinase K-Resistant conformation under physiological conditions. BIOCHEMISTRY[J]. 2007, 46(23): 7045-7053, https://www.webofscience.com/wos/woscc/full-record/WOS:000247141700036.
[34] Li, You, Hu, Yunfei, Zhang, Xinxin, Xu, Huimin, Lescop, Ewen, Xia, Bin, Jin, Changwen. Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2007, 282(15): 11078-11083, http://dx.doi.org/10.1074/jbc.M700970200.
[35] Hu, Yunfei, Li, You, Zhang, Xinxin, Guo, Xianrong, Xia, Bin, Jin, Changwen. Solution structures and backbone dynamics of a flavodoxin MioC from Escherichia coli in both apo- and holo-forms - Implications for cofactor binding and electron transfer. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2006, 281(46): 35454-35466, https://www.webofscience.com/wos/woscc/full-record/WOS:000241933700079.
[36] Hu Cuiyun, Yu Caifang, Liu Yanhua, Hou Xianhui, Liu Xiaoyun, Hu Yunfei, Jin Changwen. A Hybrid Mechanism for the Synechocystis Arsenate Reductase Revealed by Structural Snapshots during Arsenate Reduction *. The Journal of Biological Chemistry. 290(36): 22262-22273, http://dx.doi.org/10.1074/jbc.M115.659896.

   

（ 1 ） 细胞中生物大分子结构与相互作用的谱学测量, 参与, 国家级, 2020-01--2024-12
（ 2 ） 肠道菌抗酸性分子伴侣HdeA和HdeB的结构、动力学和活化的分子机理研究, 主持, 国家级, 2014-01--2017-12
（ 3 ） 黄素氧还蛋白结构、动力学和功能的核磁共振研究, 主持, 国家级, 2012-01--2012-12

（1）Molecular basis for proteasome lid assembly via PCI domains revealed by structures and interactions of yeast Rpn9   第五届海峡两岸磁共振研讨会   2015-01-18
（2）Structures and dynamics of bacterial flavodoxins   太平洋地区国际化学大会   2010-12-15