杨晟 男 博导 中国科学院分子植物科学卓越创新中心 （中国科学院合成生物学重点实验室）
本科毕业于浙江大学化工系（1995）
博士毕业于中国科学院上海生物化学研究所（2000）
中国科学院上海生命科学研究院工作至今
联系方式：syang@sibs.ac.cn
微信公众号：合成生物催化剂工程
课题组简况
新一代DNA测序与低成本DNA合成技术提供了海量基因资源与快速廉价的基因获取手段，为设计和创制工程细胞提供了近乎无限的可能性。杨晟实验室开发基于CRISPR相关系统的微生物基因组编辑工具，加速细胞构建以理解相关遗传与生化机制，据此迭代设计工程微生物，用于绿色化学、可持续农业与肠道益生菌药物。
实验室累计发表文章百余篇，基因组编辑工具质粒分发两千余次，纤维素乙醇酵母在巴西和美国投入商业应用，在燃料乙醇与氨基酸等领域取得显著经济效益。酶工程成果获上海市科技进步一等奖。杨晟研究员获DuPont Young Professor Award, Novozymes Innovation Award, 国务院政府特殊津贴及国家杰出青年科学基金。
本组现有助理4人，博士生1人，硕士生3人及10余位客座研发人员，拥有微生物分子遗传与合成生物学的完备实验设施。
招新启事
1. 硕博连读生（每年2名含博士生）
建议重点高校本科生在大三暑期前早联系早实习早了解早决定早选择。
2. 外招博士生（如果因招硕博连读生用完当年名额就只能报考华东理工大学联合培养博士生名额）
3. 博士后：职责为完成高水平论文和/或开展企业合作项目。享受上海市落户绿色通道政策，年薪面议。
毕业去向
学生/博后 | 毕业/出站时间 | 去向 | 现职 |
王晶茹 | 2004硕 | 中科院神经科学研究所 | 基石药业 资深临床科学家 |
陈茂林 | 2004硕 | 青岛汉河动植物药业有限公司 | 青岛汉河动植物药业有限公司 总经理 |
张燕 | 2004硕 | 青岛市食品药品检验研究院 | 青岛市食品药品检验研究院 科研中心主任 |
苏流利 | 2005硕 | 中科院巴斯德研究所 | 艾本德公司 产品技术支持经理 |
范文超 | 2005硕 | 横店集团 | 浙江华睿生物技术有限公司 总经理 |
林欣 | 2005硕 | 中科院巴斯德研究所 | 药明生物抗体开发 助理主任 |
夏雨 | 2007博 | 江南大学食品学院 | 江南大学食品学院 副教授 |
郑华宝 | 2007博 | 德国马普煤炭研究所 | 浙江农林大学 教授 |
王金刚 | 2008博 | 美国乔治亚理工 | 上海邦林生物技术有限公司 总经理 |
杨俊杰 | 2010博 | 中科院上海生命科学研究院 | 中科院分子植物科学卓越创新中心 副研究员 |
刘旭东 | 2011博后 | 上海莱士 | 上海莱士 资深专家 |
张仙 | 2011博后 | 美国莱斯大学 | 上海合全药物研发有限公司 副主任高级研究员 |
朱丽 | 2011博 | 上海来益生物药物研究开发中心 | 某大分子公司 主管 |
张大龙 | 2011博 | 中科院天津工业生物技术研究所 | 杭州酶因生物技术有限公司 执行董事 |
王帅 | 2011博后 | 中科院上海应用物理研究所 | 中科院上海应用物理研究所 副研究员 |
吴辉 | 2011博后 | 美国莱斯大学 | 华东理工大学 教授 |
柳鹏福 | 2011博后 | 浙江工业大学 | 浙江工业大学 助理研究员 |
李治林 | 2012博后 | 美国莱斯大学 | 南京金斯瑞 研发研究员 |
孙周通 | 2012博 | 新加坡南洋理工 | 中科院天津工业生物技术研究所 研究员 |
刁刘洋 | 2012博 | 中科院上海生命科学研究院 | 柯泰亚生物科技 总经理 |
李键 | 2012博 | 芬美意中国研发中心 | 天境生物 |
肖晗 | 2012博 | 伊利诺伊大学厄巴纳香槟分校 | 上海交通大学 副研究员 |
唐玮 | 2012硕 | 金佰利 | 雅诗兰黛全球总部（纽约）消费者互动经理 |
赵蒙 | 2012硕 | 中科院计算生物学所 | 上海休整 |
徐舒 | 2013硕 | 长宁区财税局 | 道禾长期投资 投资经理 |
张凝 | 2015博 | 睿智化学 | 安进研发中心 副研究员 |
闻志强 | 2016博后 | 南京理工大学 | 南京师范大学 讲师 |
段春兰 | 2016硕 | Selleck中国 | Selleck中国 客户经理 |
徐晓庶 | 2017博 | 斯坦福大学 | 斯坦福大学 博后 |
高书良 | 2017博 | 上海生工 | 据说在美国 |
童阳阳 | 2017硕 | 浙江医药 | 南京纽邦生物科技有限公司 研发员 |
陈晓雪 | 2017硕 | 上海罗氏制药有限公司 | 上海恒瑞 高级医药信息顾问 |
曾哲 | 2017硕 | 荷兰瓦赫宁根大学食品微生物 | 蓝晶生物 BD |
章美琴 | 2017硕 | 复旦大学上海医学院 | 上海市第九人民医院 博士后 |
刘琦 | 2017硕 | 上海交通大学生命科学学院 | 上海交通大学生命科学学院 博后 |
任晓丹 | 2018硕 | 河南平顶山市高中 | 河南平顶山市高中 生物老师 |
王心 | 2018博 | 河南工业大学 | 河南工业大学 讲师 |
杨海锋 | 2018硕 | 江苏省产业技术研究院 | 浙江华睿生物技术有限公司 研发研究员 |
江源 | 2018博 | 德国 | 德国休整中 |
李琦 | 2019博 | 四川师范大学 | 四川师范大学 讲师 |
刘金乐 | 2019博 | 郑州大学 | 郑州大学 师资博士后 |
武美贤 | 2019硕 | 淮阴师范学院 | 淮阴师范学院 辅导员 |
刘映淼 | 2019博后 | 浙江华睿生物技术有限公司 | 浙江华睿生物技术有限公司 研发研究员 |
汪庆卓 | 2020博 | 南京师范大学 | 南京师范大学 师资博士后 |
孔杨杨 | 2020硕 | 上海志学文化传播有限公司 | 上海志学文化传播有限公司 创新实验室副主任 |
孙兵兵 | 2021博后 | 中科院分子植物科学卓越创新中心 | 中科院分子植物科学卓越创新中心 助理研究员 |
发表论文
https://www.researchgate.net/profile/Sheng_Yang10
1、Qingzhuo Wang#, Huabao Zheng#, Rongsheng Tao, Qi Li, Yu Jiang*, Sheng Yang* (2022) Vitreoscilla hemoglobin enhances the catalytic performance of industrial oxidases in vitro. Applied Microbiology and Biotechnology. DOI: 10.1007/s00253-022-11974-3
2、Junjie Yang*#, Jiawei Yang#, Yiwen Zhang, Siqi Yang, Jieze Zhang, Yu Jiang, Sheng Yang* (2021) CRISPR-associated transposase system can insert multiple copies of donor DNA into the same target locus. The CRISPR Journal. 4(6):789-798
3、Jiaqi Xu, Sheng Yang*, Lirong Yang* (2021) Vibrio natriegens as a host for rapid biotechnology. Trends in biotechnology. DOI:10.1016/j.tibtech.2021.10.007
4、Qi Li, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang* (2021) Recent progress on n-butanol production by lactic acid bacteria. World Journal of Microbiology and Biotechnology . 37:205
5、Siqi Yang#, Yiwen Zhang#, Jiaqi Xu, Jiao Zhang, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang* (2021) Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration. Nucleic Acids Research. DOI: 10.1093/nar/gkab752
6、Yiwen Zhang, Jiawei Yang, Siqi Yang, Jieze Zhang, Jun Chen, Rongsheng Tao, Yu Jiang, Junjie Yang and Sheng Yang* (2021) Programming cells by multicopy chromosomal integration using CRISPR-associated transposases. The CRISPR Journal. 4(3):350-359
7、Jiaqi Xu, Junjie Yang, Yu Jiang, Mianbin Wu*, Sheng Yang*, Lirong Yang (2021) A novel global transcriptional perturbation target identified by forward genetics reprograms Vibrio natriegens for improving recombinant protein production. Acta Biochim Biophys Sin. 53(9):1124–1130
8、Tianqiong Shi, Yawen Li, Li Zhu, Yangyang Tong, Junjie Yang, Yunming Fang, Meng Wang, Jieze Zhang, Yu Jiang, Sheng Yang*(2021) Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction. Biotechnology journal. DOI: 10.1002/biot.202100097
9、Li Zhu, Jieze Zhang, Jiawei Yang, Yu Jiang, Sheng Yang*(2021) Strategies for optimizing acetyl-CoA formation from glucose in bacteria. Trends in Biotechnology. doi: 10.1016/j.tibtech.2021.04.004.
10、Qi Li, Bingbing Sun, Jun Chen, Yiwen Zhang, Yu Jiang, Sheng Yang*(2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochim Biophys Sin. 53(5):620-627.
11、Xin Wang, Bei Liao, Zhijun Li, Guangxin Liu, Liuyang Diao, Fenghui Qian, Junjie Yang, Yu Jiang, Shumiao Zhao, Youguo Li, Sheng Yang*(2021) Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae. Bioresources and Bioprocessing.8:20
12、Jiaqi Xu, Feng Dong, Meixian Wu, Rongsheng Tao, Junjie Yang, Mianbin Wu, Yu Jiang*, Sheng Yang*, Lirong Yang*(2021) Vibrio natriegens as a pET compatible expression host complementary to Escherichia coli. Frontiers in Microbiology. doi:10.3389/fmicb.2021.627181.
13、Bingbing Sun, Haiyan Liu , Yu Jiang, Lei Shao, Sheng Yang*, Daijie Chen*(2020) New Mutations Involved in Colistin Resistance in Acinetobacter baumannii. mSphere. 5(2):e00895-19
14、Qi Li, Meixian Wu, Zhiqiang Wen, Yuan Jiang, Xin Wang, Yawei Zhao, Jinle Liu, Junjie Yang, Yu Jiang, Sheng Yang*(2020) Optimization of n‑butanol synthesis in Lactobacillus brevis via the functional expression of thl, hbd, crt and te.Journal of Industrial Microbiology & Biotechnology. doi:10.1007/s10295-020-02331-2
15、Xiaoman Sun, Zixu Zhang, Lingru Wang, Jinggang Wang, Yan Liang, Haifeng Yang, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang* (2020) Downregulation of T7 RNA polymerase transcription enhances pET‐based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis. Biotechnology and Bioengineering. doi: 10.1002/bit.27558
16、Jiao Zhang, Fenghui Qian, Feng Dong, Qingzhuo Wang, Junjie Yang, Yu Jiang*, Sheng Yang* (2020) De Novo Engineering of Corynebacterium glutamicum for l-Proline Production. ACS Synth Biol. doi: 10.1021/acssynbio.0c00249
17、Yiwen Zhang, Xiaoman Sun, Qingzhuo Wang, Jiaqi Xu, Feng Dong, Siqi Yang, Jiawei Yang, Zixu Zhang, Yuan Qian, Jun Chen, Jiao Zhang, Yingmiao Liu, Rongsheng Tao, Yu Jiang, Junjie Yang*, Sheng Yang* (2020) Multicopy chromosomal integration using CRISPR-associated transposases. ACS Synth Biol. doi: 10.1021/acssynbio.0c00073
18、Jinle Liu, Yu Jiang, Jun Chen, Junjie Yang, Weihong Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang* (2020) Metabolic engineering and adaptive evolution of Clostridium beijerinckii to increase solvents production from corn stover hydrolysate. J Agric Food Chem. doi: 10.1021/acs.jafc.0c03048
19、Zhiqiang Wen, Qi Li, Jinle Liu, Mingjie Jin*, Sheng Yang* (2020) Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization. Microb Biotechnol. 13(2):410-422
20、Zhiqiang Wen, Minrui Lu, Rodrigo Ledesma-Amaro, Qi Li, Mingjie Jin*, Sheng Yang* (2020) TargeTron Technology Applicable in Solventogenic Clostridia: Revisiting 12 Years' Advances. Biotechnology journal. 15(1):e1900284
21、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Yu Jiang, Shuliang Gao, Mingjie Jin*, Sheng Yang* (2020) Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community. Biotechnology and Bioengineering. Doi: 10.1002/bit.27333
22、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Mingjie Jin*, Sheng Yang* (2020) Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing. ACS synthetic biology.Doi: 10.1021/acssynbio.9b00331
23、Qi Li, Francois M. Seys, Nigel P. Minton, Junjie Yang, Yu Jiang, Weihong Jiang, Sheng Yang*（2019）CRISPR-Cas9(D10A) nickase-assisted base editing in solvent producer Clostridium beijerinckii. Biotechnol Bioeng. Doi: 10.1002/bit.26949
24、He Huang, Xin Song, Sheng Yang*（2019） Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Biotechnology journal. doi: 10.1002/biot.201800690.
25、Zhiqiang Wen, Ledesma-Amaro, Rodrigo; Jianping Lin*, Yu Jiang, Sheng Yang*（2019）Improved n-butanol production from Clostridium cellulovorans by integrated metabolic and evolutionary engineering. Appl Environ Microbiol. Doi: 10.1128/AEM.02560-18
26、Xinyun Jing, Bingran Xie, Longxian Chen, Niubing Zhang, Yiyi Jiang, Hang Qin, Hongbing Wang, Pei Hao*, Sheng Yang* , Xuan Li*（2018）Implementation of the CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing. Nucleic Acids Res. Doi :10.1093/nar/gky433
27、Bingbing Sun, Junjie Yang, Sheng Yang*, Richard D. Ye*, Daijie Chen,*; Yu Jiang*（2018）A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Biotechnology journal . 13 (9) ：1700588
28、Xin Wang, Junjie Yang, Sheng Yang*, Yu Jiang*（2018）Unraveling the genetic basis of fast L-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae. Biotechnol Bioeng. Doi:10.1002/bit.26827
29、Jun Sun, Qingzhuo Wang, Yu Jiang, Zhiqiang Wen, Lirong Yang, Jianping Wu* and Sheng Yang*（2018）Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Microb Cell Fact. 17:41
30、Xin Song, He Huang, Zhi-Qiang Xiong, Lianzhong Ai*, Sheng Yang* (2017) CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Appl. Environ. Microbiol. doi: 10.1128/AEM.01259-17
31、Qi Liu, Yu Jiang, Lei Shao, Ping Yang, Bingbing Sun,Sheng Yang*, and Daijie Chen* (2017) CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus. Acta Biochim Biophys Sin. doi: 10.1093/abbs/gmx074
32、Xiaoshu Xu, Chao Wang, Jun Chen, and Sheng Yang* （2017）Streptomyces virginiae PPDC is a new type of phenylpyruvate decarboxylase composed of two subunits. ACS Chemical Biology 2017; doi:10.1021/acschembio.7b00307
33、Yu Jiang, Fenghui Qian, Junjie Yang, Yingmiao Liu, Feng Dong, Chongmao Xu, Bingbing Sun, Biao Chen, Xiaoshu Xu, Yan Li, Renxiao Wang, Sheng Yang* (2017) CRISPR-Cpf1 -assisted genome editing of Corynebacterium glutamicum. Nature Communications.doi:10.1038/ncomms15179 ESI高被引
34、Shuliang Gao, Yangyang Tong, Li Zhu, Ge Mei, Zhang Yian, Daijie Chen, Yu Jiang, Sheng Yang* (2017) Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous β-carotene production. Metabolic Engineering. 41:192-201
35、Zhiqiang Wen, Nigel P. Minton, Ying Zhang, Qi Li, Jinle Liu, Yu Jiang, Sheng Yang*（2017）Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering.39：38-48
36、Qingzhuo Wang, ShuangYan Tang*, Sheng Yang* (2017) Genetic biosensors for small-molecule products: Design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering. 11 (1), 15-26.
37、Junjie Yang and Sheng Yang* (2016)Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids. BMC Genomics . 18(1):940
38、Yu Jiang, Rongsheng Tao, Zhengquan Shen, Liangdong Sun, Fuyun Zhu, Sheng Yang*(2016)Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation. Appl Biochem Biotechnol.180（7） 1446-1455
39、Shuliang Gao, Yangyang Tong, Zhiqiang Wen, Li Zhu, Mei Ge, Daijie Chen*, Yu Jiang*, Sheng Yang* (2016) Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Journal of Industrial Microbiology & Biotechnology . 43（8）1085–1093
40、Yu Jiang Rongsheng Tao , Sheng Yang* (2016) Tailor-made biocatalysts enzymes for the fine chemical industry in China. Biotechnol Journal. 11,1121–1123
41、Qi Li, Jun Chen, Nigel P. Minton, Ying Zhang, Zhiqiang Wen, Jinle Liu, Haifeng Yang, Zhe Zeng, Xiaodan Ren, Junjie Yang, Yang Gu, Weihong Jiang, Yu Jiang, Sheng Yang* (2016) CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal. 11(7):961-72
42、Chen Yang, Xiang Gao, Yu Jiang, Bingbing Sun, Fang Gao, Sheng Yang*（2016）Synergy between methylerythritol phosphate pathway and mevalonate pathway for isoprene production in Escherichia coli. Metabolic Engineering, 2016, 37:79–91
43、Xiaoshu Xu, Jun Chen, Qingzhuo Wang, Chunlan Duan, Yan Li, Renxiao Wang,Sheng Yang*(2016) Mutagenesis of Key Residues in the Binding Center of l-Aspartate-b-Semialdehyde Dehydrogenase from Escherichia coli Enhances Utilization of the Cofactor NAD(H), ChemBioChem.17:56-64
44、Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, and Sheng Yang* (2015)Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl. Environ. Microbiol. 81:2506-14 ESI高被引
45、Ning Zhang, Lijun Shao, Yu Jiang, Yang Gu, Qi Li, Jinle Liu, Weihong Jiang, Sheng Yang* (2015) I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Journal Microbial Methods. 108:49-60
46、Yu Jiang, Jinle Liu, Yunliu Yang, Weihong Jiang, Sheng Yang* (2014) Current status and prospects of industrial bio-production of n-butanol in China, Biotechnology Advances. 33：1493-1501
47、Junjie Yang, Bingbing Sun, He Huang, Yu Jiang, Liuyang Diao, Biao Chen, Chongmao Xua,Xin Wang, Jinle Liu, Weihong Jiang, Sheng Yang* (2014) High-efficiency Scarless Genetic Modification in Escherichia coli Using lambda-Red Recombination and I-SceI Cleavage. Appl. Environ. Microbiol. 80(13):3826-34
48、Rongsheng Tao, Yu Jiang, Fuyun Zhu, Sheng Yang* (2014) A one-pot system for production ofL-2-aminobutyric acid from L-threonine by L-threonine deaminase and a NADH regeneration system based on L-leucine dehydrogenase and formate dehydrogenase. Biotechnology Letters 36(4):835-41
49、Liuyang Diao, Yingmiao Liu, Fenghui Qian, Junjie Yang, Yu Jiang, Sheng Yang* (2013）Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution. BMC Biotechnology 13:110
50、Zhilin Li, Han Xiao, Weihong Jiang, Yu Jiang*, Sheng Yang* (2013) Improvement of butanol production from xylose mother liquor by engineering xylose metabolic pathway in Clostridium acetobutylicum EA 2018. Applied Biochemistry and Biotechnology. 171(3):555-68
51、Han Xiao, Zhilin Li, Yu Jiang, Yunliu Yang, Weihong Jiang, Yang Gu*, Sheng Yang* (2012) Metabolic engineering of D-xylose pathway in Clostridium beijerinckii to optimize solvent production from xylose mother liquid. Metabolic Engineering 14: 565-578
52、Han Xiao, Yang Gu, Yuanyuan Ning, Yunliu Yang, W.J. Mitchell, Weihong Jiang*, Sheng Yang* (2011) Confirmation and elimination of xylose-metabolic bottlenecks in glucose-PTS-deficient Clostridium acetobutylicum to realize simultaneous utilization of glucose, xylose and arabinose. Appl. Environ. Microbiol 77:7886-7895
53、Zhoutong Sun, Yuanyuan Ning, Lixia Liu, Yingmiao Liu, Bingbing Sun, Weihong Jiang, Chen Yang and Sheng Yang* (2011) Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microbial Cell Factories. 10:71 Highly Accessed
54、Li Zhu, Rongsheng Tao, Yi Wang, Yu Jiang, Xin Lin, Yunliu Yang, Huabao Zheng, Weihong Jiang, Sheng Yang* (2011) Removal of L-alanine from the production of L-2-aminobutyric acid by introduction of alanine racemase and D-amino acid oxidase. Appl Microbiol Biotechnol. 90:903-910
55、Jian LI, Jingbo Zhao, Meng Zhao, Yunliu Yang, Weihong Jiang, Sheng Yang* (2010)Screening and Characterization of Butanol Tolerant Microorganisms. Letters in Applied Microbiology 50: 373-9
56、Yu Jiang, Chongmao Xu, Feng Dong, Yunliu Yang, Weihong Jiang*, Sheng Yang* (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metabolic Engineering 11: 284-91
57、Junjie Yang, Weihong Jiang, Sheng Yang* (2009). mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Research. 9(4): 600-9
58、Hong Yu, Jian Li, Dalong Zhang, Yunliu Yang, Weihong Jiang, Sheng Yang* (2009). Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR. Appl. Microbiol. Biotechnol. 82(2): 279-85
59、Lijun Shao, Shiyuan Hu, Yi Yang, Yang Gu, Jun Chen, Yunliu Yang, Weihong Jiang*, Sheng Yang* (2007) Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Research. 17: 963-5