基本信息

杨晟  男  博导  中国科学院分子植物科学卓越创新中心 (中国科学院合成生物学重点实验室)

本科毕业于浙江大学化工系(1995)
博士毕业于中国科学院上海生物化学研究所(2000)
中国科学院上海生命科学研究院工作至今
联系方式:syang@sibs.ac.cn
微信公众号:合成生物催化剂工程



课题组简况

新一代DNA测序与低成本DNA合成技术提供了海量基因资源与快速廉价的基因获取手段,为设计和创制工程细胞提供了近乎无限的可能性。杨晟实验室开发基于CRISPR相关系统的微生物基因组编辑工具,加速细胞构建以理解相关遗传与生化机制,据此迭代设计工程微生物,用于绿色化学、可持续农业与肠道益生菌药物。

实验室累计发表文章百余篇,基因组编辑工具质粒分发两千余次,纤维素乙醇酵母在美国、巴西和印度投入商业应用,在酒精与氨基酸等领域取得显著经济效益。酶工程成果获上海市科技进步一等奖。杨晟研究员获DuPont Young Professor Award, Novozymes Innovation Award, 国务院政府特殊津贴及国家杰出青年科学基金。

本组现有助理4人,博士生1人,硕士生3人及10余位客座研发人员,拥有微生物分子遗传与合成生物学的完备实验设施。


CRISPR转座讲座视频

酶库

菌株质粒:

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招新启事

1. 硕博连读生:每三年2名含博士生,另有中科大联合培养硕博连读生名额。

建议重点高校本科生在大三暑期前早联系早实习早了解早决定早选择。

2. 外招博士生:我们微生物专业一般4-5位招生导师,每位导师从报考TA的初试考生中推出1名,竞争一个国科大外招博士生名额和两个华东理工大学联合培养博士生名额。如果导师因招硕博连读生(两年3名)用完当年名额,考生就只能报考华东理工大学联合培养博士生名额(除学位为华东理工大学授予,其他均同国科大学生)。因2023年硕博连读生中我已有两位意向同学,2023年外招博只有华东理工大学联培名额。

3. 特别研究助理/博士后:职责为完成高水平论文和/或开展企业合作项目。享受上海市落户绿色通道政策,年薪20-40万。


毕业去向


学生/博后

毕业/出站时间

去向

现职

王晶茹

2004

中科院神经科学研究所

基石药业   资深临床科学家

陈茂林

2004

青岛汉河动植物药业有限公司

青岛汉河动植物药业有限公司   总经理

张燕

2004

青岛市食品药品检验研究院

青岛市食品药品检验研究院   科研中心主任

苏流利

2005

中科院巴斯德研究所

艾本德公司   产品技术支持经理

范文超

2005

横店集团

浙江华睿生物技术有限公司   总经理

林欣

2005

中科院巴斯德研究所

药明生物抗体开发   助理主任

夏雨

2007

江南大学食品学院

江南大学食品学院   副教授

郑华宝

2007

德国马普煤炭研究所

浙江农林大学   教授

王金刚

2008

美国乔治亚理工

上海邦林生物技术有限公司   总经理

杨俊杰

2010

中科院上海生命科学研究院

中科院分子植物科学卓越创新中心   副研究员

刘旭东

2011博后

上海莱士

上海莱士   资深专家

张仙

2011博后

美国莱斯大学

苏州百福安酶技术有限公司 CTO

朱丽

2011

上海来益生物药物研究开发中心

和度生物 高级主管

张大龙

2011

中科院天津工业生物技术研究所

杭州酶因生物技术有限公司   执行董事

王帅

2011博后

中科院上海应用物理研究所

中科院上海应用物理研究所   副研究员

吴辉

2011博后

美国莱斯大学

华东理工大学   教授

柳鹏福

2011博后

浙江工业大学

浙江工业大学   助理研究员

李治林

2012博后

美国莱斯大学

-

孙周通

2012

新加坡南洋理工

中科院天津工业生物技术研究所   研究员

刁刘洋

2012

中科院上海生命科学研究院

柯泰亚生物科技   总经理

李键

2012

芬美意中国研发中心

天境生物

肖晗

2012

伊利诺伊大学厄巴纳香槟分校

上海交通大学   副研究员

唐玮

2012

金佰利

雅诗兰黛全球总部(纽约)消费者互动经理

赵蒙

2012

中科院计算生物学所

上海休整

徐舒

2013

长宁区财税局

道禾长期投资   投资经理

张凝

2015

睿智化学

基石药业

闻志强

2016博后

南京理工大学

南京师范大学  副教授 即将离职创业

段春兰

2016

Selleck中国

Selleck中国 客户经理

徐晓庶

2017

斯坦福大学

斯坦福大学   博后

高书良

2017

上海生工

据说在美国

童阳阳

2017

浙江医药

南京纽邦生物科技有限公司   研发员

陈晓雪

2017

上海罗氏制药有限公司

上海恒瑞   高级医药信息顾问

曾哲

2017

荷兰瓦赫宁根大学食品微生物

达能 科学家

章美琴

2017

复旦大学上海医学院

上海市第九人民医院   博士后

刘琦

2017

上海交通大学生命科学学院

上海交通大学生命科学学院   博后

任晓丹

2018

河南平顶山市高中

河南平顶山市高中   生物教师

王心

2018

河南工业大学

河南工业大学   讲师

杨海锋

2018

江苏省产业技术研究院

之江实验室 工程专员

江源

2018

德国

德国休整中

李琦

2019

四川师范大学

四川师范大学   讲师

刘金乐

2019

郑州大学

郑州大学   师资博士后

武美贤

2019

淮阴师范学院

淮阴师范学院   辅导员

刘映淼

2019博后

浙江华睿生物技术有限公司

浙江华睿生物技术有限公司 研发研究员

汪庆卓

2020

南京师范大学

南京师范大学   师资博士后

孔杨杨

2020

上海志学文化传播有限公司

​跳槽中

孙兵兵

2021博后

中科院分子植物科学卓越创新中心

中科院分子植物科学卓越创新中心   助理研究员

张姣

2022

迪辅乐生物(上海) 

迪辅乐生物(上海) 科学家 

张译文

2022

中科院天津工业生物技术研究所

中科院天津工业生物技术研究所   博后

吴联

2022

中科院有机化学研究所

中科院有机化学研究所   助理研究员

 


发表论文

     https://www.researchgate.net/profile/Sheng_Yang10

    1、Zhiqiang Wen, Jun Chen, Yunliu Yang, Ying Zhang, Yu Jiang*, Sheng Yang* (2022) Pilot-scale fermentation of 300 t/a total solvents from sweet sorghum stalk juice. Chemical Engineering Journal. DOI:https://doi.org/10.1016/j.cej.2022.140534

    2、Yu Jiang#*, Bingbing Sun#, Fenghui Qian , Feng Dong, Chongmao Xu , Wuling Zhong, Rui Huang , Qiwei Zhai , Sheng Yang* (2022) Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a living therapeutic for phenylketonuria. SCIENCE CHINA Life Sciences. DOI: 10.1007/s11427-021-2137-3

    3、Wuling Zhong, Hui Wang, Yale Yang, Yali Zhang, Hejin Lai, Yalan Cheng, Huimin Yu, Ning Feng , Rui Huang, Shen Liu, Sheng Yang, Tongyang Hao, Baoyu Zhang, Hao Ying, Fang Zhang, Feifan Guo, Qiwei Zhai* (2022) High-protein diet prevents fat mass increase after dieting by counteracting Lactobacillus-enhanced lipid absorption. Nature Metabolism. 4:1713–1731

    4、Qingzhuo Wang#, Huabao Zheng#, Rongsheng Tao, Qi Li, Yu Jiang*, Sheng Yang* (2022) Vitreoscilla hemoglobin enhances the catalytic performance of industrial oxidases in vitro. Applied Microbiology and Biotechnology. DOI: 10.1007/s00253-022-11974-3

    5、Junjie Yang*#, Jiawei Yang#, Yiwen Zhang, Siqi Yang, Jieze Zhang, Yu Jiang, Sheng Yang* (2021) CRISPR-associated transposase system can insert multiple copies of donor DNA into the same target locus. The CRISPR Journal. 4(6):789-798

    6、Jiaqi Xu, Sheng Yang*, Lirong Yang* (2021) Vibrio natriegens as a host for rapid biotechnology. Trends in Biotechnology. DOI:10.1016/j.tibtech.2021.10.007

    7、Qi Li, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang* (2021) Recent progress on n-butanol production by lactic acid bacteria. World Journal of Microbiology and Biotechnology . 37:205

    8、Siqi Yang#, Yiwen Zhang#, Jiaqi Xu, Jiao Zhang, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang* (2021) Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration. Nucleic Acids Research. DOI: 10.1093/nar/gkab752

    9、Yiwen Zhang, Jiawei Yang, Siqi Yang, Jieze Zhang,  Jun Chen,  Rongsheng Tao, Yu Jiang, Junjie Yang and Sheng Yang* (2021) Programming cells by multicopy chromosomal integration using CRISPR-associated transposases. The CRISPR Journal. 4(3):350-359

    10、Jiaqi Xu, Junjie Yang, Yu Jiang, Mianbin Wu*, Sheng Yang*, Lirong Yang (2021) A novel global transcriptional perturbation target identified by forward genetics reprograms Vibrio natriegens for improving recombinant protein production. Acta Biochim Biophys Sin. 53(9):1124–1130

    11、Tianqiong Shi, Yawen Li, Li Zhu, Yangyang Tong, Junjie Yang, Yunming Fang, Meng Wang, Jieze Zhang, Yu Jiang, Sheng Yang*(2021) Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction. Biotechnology journal. DOI: 10.1002/biot.202100097

    12、Li Zhu, Jieze Zhang, Jiawei Yang, Yu Jiang, Sheng Yang*(2021) Strategies for optimizing acetyl-CoA formation from glucose in bacteria. Trends in Biotechnology. doi: 10.1016/j.tibtech.2021.04.004.

    13、Qi Li, Bingbing Sun, Jun Chen, Yiwen Zhang, Yu Jiang, Sheng Yang*(2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochim Biophys Sin53(5):620-627.

    14、Xin Wang, Bei Liao, Zhijun Li, Guangxin Liu, Liuyang Diao, Fenghui Qian, Junjie Yang, Yu Jiang, Shumiao Zhao, Youguo Li, Sheng Yang*(2021) Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae. Bioresources and Bioprocessing.8:20

    15、Jiaqi Xu, Feng Dong, Meixian Wu, Rongsheng Tao, Junjie Yang, Mianbin Wu, Yu Jiang*, Sheng Yang*, Lirong Yang*(2021) Vibrio natriegens as a pET compatible expression host complementary to Escherichia coli. Frontiers in Microbiology. doi:10.3389/fmicb.2021.627181.

    16、Bingbing Sun, Haiyan Liu , Yu Jiang, Lei Shao, Sheng Yang*, Daijie Chen*(2020) New Mutations Involved in Colistin Resistance in Acinetobacter baumannii. mSphere. 5(2):e00895-19

    17、Qi Li, Meixian Wu, Zhiqiang Wen, Yuan Jiang, Xin Wang, Yawei Zhao, Jinle Liu, Junjie Yang, Yu Jiang, Sheng Yang*(2020) Optimization of n‑butanol synthesis in Lactobacillus brevis via the functional expression of thl, hbd, crt and te.Journal of Industrial Microbiology & Biotechnology. doi:10.1007/s10295-020-02331-2

    18、Xiaoman Sun, Zixu Zhang, Lingru Wang, Jinggang Wang, Yan Liang, Haifeng Yang, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang* (2020) Downregulation of T7 RNA polymerase transcription enhances pET‐based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis. Biotechnology and Bioengineering. doi: 10.1002/bit.27558

    19、Jiao Zhang, Fenghui Qian, Feng Dong, Qingzhuo Wang, Junjie Yang, Yu Jiang*, Sheng Yang* (2020) De Novo Engineering of Corynebacterium glutamicum for l-Proline Production. ACS Synth Biol. doi: 10.1021/acssynbio.0c00249

    20、Yiwen Zhang, Xiaoman Sun, Qingzhuo Wang, Jiaqi Xu, Feng Dong, Siqi Yang, Jiawei Yang, Zixu Zhang, Yuan Qian, Jun Chen, Jiao Zhang, Yingmiao Liu, Rongsheng Tao, Yu Jiang, Junjie Yang*, Sheng Yang* (2020) Multicopy chromosomal integration using CRISPR-associated transposases. ACS Synth Biol. doi: 10.1021/acssynbio.0c00073

    21、Jinle Liu, Yu Jiang, Jun Chen, Junjie Yang, Weihong Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang* (2020) Metabolic engineering and adaptive evolution of Clostridium beijerinckii to increase solvents production from corn stover hydrolysate. J Agric Food Chem. doi: 10.1021/acs.jafc.0c03048

    22、Zhiqiang Wen, Qi Li, Jinle Liu, Mingjie Jin*, Sheng Yang* (2020) Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization. Microb Biotechnol. 13(2):410-422

    23、Zhiqiang Wen, Minrui Lu, Rodrigo Ledesma-Amaro, Qi Li, Mingjie Jin*, Sheng Yang* (2020) TargeTron Technology Applicable in Solventogenic Clostridia: Revisiting 12 Years' Advances. Biotechnology journal. 15(1):e1900284

    24、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Yu Jiang, Shuliang Gao, Mingjie Jin*, Sheng Yang* (2020) Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community. Biotechnology and Bioengineering. Doi: 10.1002/bit.27333

    25、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Mingjie Jin*, Sheng Yang* (2020) Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing. ACS synthetic biology.Doi: 10.1021/acssynbio.9b00331

    26、Qi Li, Francois M. Seys, Nigel P. Minton, Junjie Yang, Yu Jiang, Weihong Jiang, Sheng Yang*(2019)CRISPR-Cas9(D10A) nickase-assisted base editing in solvent producer Clostridium beijerinckii. Biotechnol Bioeng. Doi: 10.1002/bit.26949

    27、He Huang, Xin Song, Sheng Yang*2019 Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Biotechnology journal. doi: 10.1002/biot.201800690.

    28、Zhiqiang Wen, Ledesma-Amaro, Rodrigo; Jianping Lin*, Yu Jiang, Sheng Yang*(2019)Improved n-butanol production from Clostridium cellulovorans by integrated metabolic and evolutionary engineering. Appl Environ Microbiol. Doi: 10.1128/AEM.02560-18

    29、杨萍 ,刘蔷,蒋宇,孙兵兵,杨俊杰,李琦,杨晟*,陈代杰*,(2019)耐甲氧西林金黄色葡萄球菌MRSA252辅酶A二硫化物还原酶缺失延缓苯唑西林的次级损伤效应,微生物学报,5912):2296-2305

    30、杨萍,孙兵兵,杨俊杰,杨晟*, 蒋宇*,(2018)CRISPR-Cas系统的抗耐药菌应用,中国抗生素杂志, 43(8):927-931

    31、李琦,武美贤,郭清华,邵悠然,杨俊杰,蒋宇*,杨晟*,(2018)细菌基因组编辑技术进展,生命科学,10.13376/j.cbls/2019201

    32、Xinyun Jing, Bingran Xie, Longxian Chen, Niubing Zhang, Yiyi Jiang, Hang Qin, Hongbing Wang, Pei Hao*, Sheng Yang* , Xuan Li*(2018)Implementation of the CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing. Nucleic Acids Res. Doi :10.1093/nar/gky433


    33、Bingbing Sun, Junjie Yang, Sheng Yang*, Richard D. Ye*, Daijie Chen,*; Yu Jiang*(2018)A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Biotechnology journal . 13 (9) :1700588


    34、Xin Wang, Junjie Yang, Sheng Yang*, Yu Jiang*(2018)Unraveling the genetic basis of fast L-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae. Biotechnol Bioeng. Doi:10.1002/bit.26827

    35、Jun Sun, Qingzhuo Wang, Yu Jiang, Zhiqiang Wen, Lirong Yang, Jianping Wu* and Sheng Yang*(2018)Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Microb Cell Fact. 17:41

    36、Xin Song, He Huang, Zhi-Qiang Xiong, Lianzhong Ai*, Sheng Yang* (2017) CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Appl. Environ. Microbiol. doi: 10.1128/AEM.01259-17

    37、Qi Liu, Yu Jiang, Lei Shao, Ping Yang, Bingbing Sun,Sheng Yang*, and Daijie Chen* (2017) CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus. Acta Biochim Biophys Sin. doi: 10.1093/abbs/gmx074

    38、Xiaoshu Xu, Chao Wang, Jun Chen, and Sheng Yang* (2017)Streptomyces virginiae PPDC is a new type of phenylpyruvate decarboxylase composed of two subunits. ACS Chemical Biology 2017; doi:10.1021/acschembio.7b00307

    39、Yu Jiang, Fenghui Qian, Junjie Yang, Yingmiao Liu, Feng Dong, Chongmao Xu, Bingbing Sun, Biao Chen, Xiaoshu Xu, Yan Li, Renxiao Wang, Sheng Yang* (2017) CRISPR-Cpf1 -assisted genome editing of Corynebacterium glutamicum. Nature Communications.doi:10.1038/ncomms15179 ESI高被引

    40、Shuliang Gao, Yangyang Tong, Li Zhu, Ge Mei, Zhang Yian, Daijie Chen, Yu Jiang, Sheng Yang* (2017) Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous β-carotene production. Metabolic Engineering. 41:192-201

    41、Zhiqiang Wen, Nigel P. Minton, Ying Zhang, Qi Li, Jinle Liu, Yu Jiang, Sheng Yang*(2017)Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering.39:38-48

    42、Qingzhuo Wang, ShuangYan Tang*, Sheng Yang* (2017) Genetic biosensors for small-molecule products: Design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering. 11 (1), 15-26.

    43、Junjie Yang and Sheng Yang* (2016)Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids. BMC Genomics . 18(1):940

    44、Yu Jiang, Rongsheng Tao, Zhengquan Shen, Liangdong Sun, Fuyun Zhu, Sheng Yang*(2016)Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation. Appl Biochem Biotechnol.180(7) 1446-1455

    45、Shuliang Gao, Yangyang Tong, Zhiqiang Wen, Li Zhu, Mei Ge, Daijie Chen*, Yu Jiang*, Sheng Yang* (2016) Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Journal of Industrial Microbiology & Biotechnology . 43(8)1085–1093

    46、Yu Jiang Rongsheng Tao , Sheng Yang* (2016) Tailor-made biocatalysts enzymes for the fine chemical industry in China. Biotechnol Journal. 11,1121–1123

    47、Qi Li, Jun Chen, Nigel P. Minton, Ying Zhang, Zhiqiang Wen, Jinle Liu, Haifeng Yang, Zhe Zeng, Xiaodan Ren, Junjie Yang, Yang Gu, Weihong Jiang, Yu Jiang, Sheng Yang* (2016) CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal. 11(7):961-72

    48、Chen Yang, Xiang Gao, Yu Jiang, Bingbing Sun, Fang Gao, Sheng Yang*(2016)Synergy between methylerythritol phosphate pathway and mevalonate pathway for isoprene production in Escherichia coli. Metabolic Engineering, 2016, 37:79–91

    49、Xiaoshu Xu, Jun Chen, Qingzhuo Wang, Chunlan Duan, Yan Li, Renxiao Wang,Sheng Yang*(2016) Mutagenesis of Key Residues in the Binding Center of l-Aspartate-b-Semialdehyde Dehydrogenase from Escherichia coli Enhances Utilization of the Cofactor NAD(H), ChemBioChem.17:56-64

    50、Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, and Sheng Yang* (2015)Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl. Environ. Microbiol. 81:2506-14  ESI高被引

    51、Ning Zhang, Lijun Shao, Yu Jiang, Yang Gu, Qi Li, Jinle Liu, Weihong Jiang, Sheng Yang* (2015) I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Journal Microbial Methods. 108:49-60

    52、Yu Jiang, Jinle Liu, Yunliu Yang, Weihong Jiang, Sheng Yang* (2014) Current status and prospects of industrial bio-production of n-butanol in China, Biotechnology Advances. 33:1493-1501

    53、Junjie Yang, Bingbing Sun, He Huang, Yu Jiang, Liuyang Diao, Biao Chen, Chongmao Xua,Xin Wang, Jinle Liu, Weihong Jiang, Sheng Yang* (2014) High-efficiency Scarless Genetic Modification in Escherichia coli Using lambda-Red Recombination and I-SceI Cleavage. Appl. Environ. Microbiol. 80(13):3826-34

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