新一代DNA测序与低成本DNA合成技术提供了海量基因资源与快速廉价的基因获取手段，为设计和创制工程细胞提供了近乎无限的可能性。杨晟实验室开发基于CRISPR相关系统的微生物基因组编辑工具，加速细胞构建以理解相关遗传与生化机制，据此迭代设计工程微生物，用于绿色化学、可持续农业与肠道益生菌药物。

实验室累计发表文章百余篇，基因组编辑工具质粒分发两千余次，纤维素乙醇酵母在美国、巴西和印度投入商业应用，在酒精与氨基酸等领域取得显著经济效益。酶工程成果获上海市科技进步一等奖。杨晟研究员获DuPont Young Professor Award, Novozymes Innovation Award, 国务院政府特殊津贴及国家杰出青年科学基金。

本组现有助理4人，博士生1人，硕士生3人及10余位客座研发人员，拥有微生物分子遗传与合成生物学的完备实验设施。‍‍‍‍‍‍

CRISPR转座讲座视频

酶库

菌株质粒：

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1. 硕博连读生：每三年2名含博士生，另有中科大联合培养硕博连读生名额。

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建议重点高校本科生在大三暑期前早联系早实习早了解早决定早选择。‍‍‍‍‍‍‍‍‍‍

2. 外招博士生：我们微生物专业一般4-5位招生导师，每位导师从报考TA的初试考生中推出1名，竞争一个国科大外招博士生名额和两个华东理工大学联合培养博士生名额。如果导师因招硕博连读生（两年3名）用完当年名额，考生就只能报考华东理工大学联合培养博士生名额（除学位为华东理工大学授予，其他均同国科大学生）。因2023年硕博连读生中我已有两位意向同学，2023年外招博只有华东理工大学联培名额。

3. 特别研究助理/博士后：职责为完成高水平论文和/或开展企业合作项目。享受上海市落户绿色通道政策，年薪20-40万。

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 https://www.researchgate.net/profile/Sheng_Yang10

1、Zhiqiang Wen, Jun Chen, Yunliu Yang, Ying Zhang, Yu Jiang*, Sheng Yang* (2022) Pilot-scale fermentation of 300 t/a total solvents from sweet sorghum stalk juice. Chemical Engineering Journal. DOI:https://doi.org/10.1016/j.cej.2022.140534

2、Yu Jiang#*, Bingbing Sun#, Fenghui Qian , Feng Dong, Chongmao Xu , Wuling Zhong, Rui Huang , Qiwei Zhai , Sheng Yang* (2022) Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a living therapeutic for phenylketonuria. SCIENCE CHINA Life Sciences. DOI: 10.1007/s11427-021-2137-3

3、Wuling Zhong, Hui Wang, Yale Yang, Yali Zhang, Hejin Lai, Yalan Cheng, Huimin Yu, Ning Feng , Rui Huang, Shen Liu, Sheng Yang, Tongyang Hao, Baoyu Zhang, Hao Ying, Fang Zhang, Feifan Guo, Qiwei Zhai* (2022) High-protein diet prevents fat mass increase after dieting by counteracting Lactobacillus-enhanced lipid absorption. Nature Metabolism. 4:1713–1731

4、Qingzhuo Wang#, Huabao Zheng#, Rongsheng Tao, Qi Li, Yu Jiang*, Sheng Yang* (2022) Vitreoscilla hemoglobin enhances the catalytic performance of industrial oxidases in vitro. Applied Microbiology and Biotechnology. DOI: 10.1007/s00253-022-11974-3

5、Junjie Yang*#, Jiawei Yang#, Yiwen Zhang, Siqi Yang, Jieze Zhang, Yu Jiang, Sheng Yang* (2021) CRISPR-associated transposase system can insert multiple copies of donor DNA into the same target locus. The CRISPR Journal. 4(6):789-798

6、Jiaqi Xu, Sheng Yang*, Lirong Yang* (2021) Vibrio natriegens as a host for rapid biotechnology. Trends in Biotechnology. DOI:10.1016/j.tibtech.2021.10.007

7、Qi Li, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang* (2021) Recent progress on n-butanol production by lactic acid bacteria. World Journal of Microbiology and Biotechnology . 37:205

8、Siqi Yang#, Yiwen Zhang#, Jiaqi Xu, Jiao Zhang, Jieze Zhang, Junjie Yang, Yu Jiang, Sheng Yang* (2021) Orthogonal CRISPR-associated transposases for parallel and multiplexed chromosomal integration. Nucleic Acids Research. DOI: 10.1093/nar/gkab752

9、Yiwen Zhang, Jiawei Yang, Siqi Yang, Jieze Zhang,  Jun Chen,  Rongsheng Tao, Yu Jiang, Junjie Yang and Sheng Yang* (2021) Programming cells by multicopy chromosomal integration using CRISPR-associated transposases. The CRISPR Journal. 4(3):350-359

10、Jiaqi Xu, Junjie Yang, Yu Jiang, Mianbin Wu*, Sheng Yang*, Lirong Yang (2021) A novel global transcriptional perturbation target identified by forward genetics reprograms Vibrio natriegens for improving recombinant protein production. Acta Biochim Biophys Sin. 53(9):1124–1130

11、Tianqiong Shi, Yawen Li, Li Zhu, Yangyang Tong, Junjie Yang, Yunming Fang, Meng Wang, Jieze Zhang, Yu Jiang, Sheng Yang*(2021) Engineering the oleaginous yeast Yarrowia lipolytica for β-farnesene overproduction. Biotechnology journal. DOI: 10.1002/biot.202100097

12、Li Zhu, Jieze Zhang, Jiawei Yang, Yu Jiang, Sheng Yang*(2021) Strategies for optimizing acetyl-CoA formation from glucose in bacteria. Trends in Biotechnology. doi: 10.1016/j.tibtech.2021.04.004.

13、Qi Li, Bingbing Sun, Jun Chen, Yiwen Zhang, Yu Jiang, Sheng Yang*(2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochim Biophys Sin. 53(5):620-627.

14、Xin Wang, Bei Liao, Zhijun Li, Guangxin Liu, Liuyang Diao, Fenghui Qian, Junjie Yang, Yu Jiang, Shumiao Zhao, Youguo Li, Sheng Yang*(2021) Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae. Bioresources and Bioprocessing.8:20

15、Jiaqi Xu, Feng Dong, Meixian Wu, Rongsheng Tao, Junjie Yang, Mianbin Wu, Yu Jiang*, Sheng Yang*, Lirong Yang*(2021) Vibrio natriegens as a pET compatible expression host complementary to Escherichia coli. Frontiers in Microbiology. doi:10.3389/fmicb.2021.627181.

16、Bingbing Sun, Haiyan Liu , Yu Jiang, Lei Shao, Sheng Yang*, Daijie Chen*(2020) New Mutations Involved in Colistin Resistance in Acinetobacter baumannii. mSphere. 5(2):e00895-19

17、Qi Li, Meixian Wu, Zhiqiang Wen, Yuan Jiang, Xin Wang, Yawei Zhao, Jinle Liu, Junjie Yang, Yu Jiang, Sheng Yang*(2020) Optimization of n‑butanol synthesis in Lactobacillus brevis via the functional expression of thl, hbd, crt and te.Journal of Industrial Microbiology & Biotechnology. doi:10.1007/s10295-020-02331-2

18、Xiaoman Sun, Zixu Zhang, Lingru Wang, Jinggang Wang, Yan Liang, Haifeng Yang, Rongsheng Tao, Yu Jiang, Junjie Yang, Sheng Yang* (2020) Downregulation of T7 RNA polymerase transcription enhances pET‐based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis. Biotechnology and Bioengineering. doi: 10.1002/bit.27558

19、Jiao Zhang, Fenghui Qian, Feng Dong, Qingzhuo Wang, Junjie Yang, Yu Jiang*, Sheng Yang* (2020) De Novo Engineering of Corynebacterium glutamicum for l-Proline Production. ACS Synth Biol. doi: 10.1021/acssynbio.0c00249

20、Yiwen Zhang, Xiaoman Sun, Qingzhuo Wang, Jiaqi Xu, Feng Dong, Siqi Yang, Jiawei Yang, Zixu Zhang, Yuan Qian, Jun Chen, Jiao Zhang, Yingmiao Liu, Rongsheng Tao, Yu Jiang, Junjie Yang*, Sheng Yang* (2020) Multicopy chromosomal integration using CRISPR-associated transposases. ACS Synth Biol. doi: 10.1021/acssynbio.0c00073

21、Jinle Liu, Yu Jiang, Jun Chen, Junjie Yang, Weihong Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang* (2020) Metabolic engineering and adaptive evolution of Clostridium beijerinckii to increase solvents production from corn stover hydrolysate. J Agric Food Chem. doi: 10.1021/acs.jafc.0c03048

22、Zhiqiang Wen, Qi Li, Jinle Liu, Mingjie Jin*, Sheng Yang* (2020) Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization. Microb Biotechnol. 13(2):410-422

23、Zhiqiang Wen, Minrui Lu, Rodrigo Ledesma-Amaro, Qi Li, Mingjie Jin*, Sheng Yang* (2020) TargeTron Technology Applicable in Solventogenic Clostridia: Revisiting 12 Years' Advances. Biotechnology journal. 15(1):e1900284

24、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Yu Jiang, Shuliang Gao, Mingjie Jin*, Sheng Yang* (2020) Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community. Biotechnology and Bioengineering. Doi: 10.1002/bit.27333

25、Zhiqiang Wen, Rodrigo Ledesma-Amaro, Minrui Lu, Mingjie Jin*, Sheng Yang* (2020) Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing. ACS synthetic biology.Doi: 10.1021/acssynbio.9b00331

26、Qi Li, Francois M. Seys, Nigel P. Minton, Junjie Yang, Yu Jiang, Weihong Jiang, Sheng Yang*（2019）CRISPR-Cas9(D10A) nickase-assisted base editing in solvent producer Clostridium beijerinckii. Biotechnol Bioeng. Doi: 10.1002/bit.26949

27、He Huang, Xin Song, Sheng Yang*（2019） Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus. Biotechnology journal. doi: 10.1002/biot.201800690.

28、Zhiqiang Wen, Ledesma-Amaro, Rodrigo; Jianping Lin*, Yu Jiang, Sheng Yang*（2019）Improved n-butanol production from Clostridium cellulovorans by integrated metabolic and evolutionary engineering. Appl Environ Microbiol. Doi: 10.1128/AEM.02560-18

29、杨萍 ，刘蔷，蒋宇，孙兵兵，杨俊杰，李琦，杨晟*，陈代杰*，（2019）耐甲氧西林金黄色葡萄球菌MRSA252辅酶A二硫化物还原酶缺失延缓苯唑西林的次级损伤效应，微生物学报，59（12）：2296-2305

30、杨萍，孙兵兵，杨俊杰，杨晟*， 蒋宇*，（2018）CRISPR-Cas系统的抗耐药菌应用，中国抗生素杂志， 43（8）:927-931

31、李琦，武美贤，郭清华，邵悠然，杨俊杰，蒋宇*，杨晟*，（2018）细菌基因组编辑技术进展，生命科学，10.13376/j.cbls/2019201

32、Xinyun Jing, Bingran Xie, Longxian Chen, Niubing Zhang, Yiyi Jiang, Hang Qin, Hongbing Wang, Pei Hao*, Sheng Yang* , Xuan Li*（2018）Implementation of the CRISPR-Cas13a system in fission yeast and its repurposing for precise RNA editing. Nucleic Acids Res. Doi :10.1093/nar/gky433




33、Bingbing Sun, Junjie Yang, Sheng Yang*, Richard D. Ye*, Daijie Chen,*; Yu Jiang*（2018）A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Biotechnology journal . 13 (9) ：1700588




34、Xin Wang, Junjie Yang, Sheng Yang*, Yu Jiang*（2018）Unraveling the genetic basis of fast L-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae. Biotechnol Bioeng. Doi:10.1002/bit.26827

35、Jun Sun, Qingzhuo Wang, Yu Jiang, Zhiqiang Wen, Lirong Yang, Jianping Wu* and Sheng Yang*（2018）Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Microb Cell Fact. 17:41

36、Xin Song, He Huang, Zhi-Qiang Xiong, Lianzhong Ai*, Sheng Yang* (2017) CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Appl. Environ. Microbiol. doi: 10.1128/AEM.01259-17

37、Qi Liu, Yu Jiang, Lei Shao, Ping Yang, Bingbing Sun,Sheng Yang*, and Daijie Chen* (2017) CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus. Acta Biochim Biophys Sin. doi: 10.1093/abbs/gmx074

38、Xiaoshu Xu, Chao Wang, Jun Chen, and Sheng Yang* （2017）Streptomyces virginiae PPDC is a new type of phenylpyruvate decarboxylase composed of two subunits. ACS Chemical Biology 2017; doi:10.1021/acschembio.7b00307

39、Yu Jiang, Fenghui Qian, Junjie Yang, Yingmiao Liu, Feng Dong, Chongmao Xu, Bingbing Sun, Biao Chen, Xiaoshu Xu, Yan Li, Renxiao Wang, Sheng Yang* (2017) CRISPR-Cpf1 -assisted genome editing of Corynebacterium glutamicum. Nature Communications.doi:10.1038/ncomms15179 ESI高被引

40、Shuliang Gao, Yangyang Tong, Li Zhu, Ge Mei, Zhang Yian, Daijie Chen, Yu Jiang, Sheng Yang* (2017) Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous β-carotene production. Metabolic Engineering. 41:192-201

41、Zhiqiang Wen, Nigel P. Minton, Ying Zhang, Qi Li, Jinle Liu, Yu Jiang, Sheng Yang*（2017）Enhanced solvent production by metabolic engineering of a twin-clostridial consortium. Metabolic Engineering.39：38-48

42、Qingzhuo Wang, ShuangYan Tang*, Sheng Yang* (2017) Genetic biosensors for small-molecule products: Design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering. 11 (1), 15-26.

43、Junjie Yang and Sheng Yang* (2016)Comparative analysis of Corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids. BMC Genomics . 18(1):940

44、Yu Jiang, Rongsheng Tao, Zhengquan Shen, Liangdong Sun, Fuyun Zhu, Sheng Yang*(2016)Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation. Appl Biochem Biotechnol.180（7） 1446-1455

45、Shuliang Gao, Yangyang Tong, Zhiqiang Wen, Li Zhu, Mei Ge, Daijie Chen*, Yu Jiang*, Sheng Yang* (2016) Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Journal of Industrial Microbiology & Biotechnology . 43（8）1085–1093

46、Yu Jiang Rongsheng Tao , Sheng Yang* (2016) Tailor-made biocatalysts enzymes for the fine chemical industry in China. Biotechnol Journal. 11,1121–1123

47、Qi Li, Jun Chen, Nigel P. Minton, Ying Zhang, Zhiqiang Wen, Jinle Liu, Haifeng Yang, Zhe Zeng, Xiaodan Ren, Junjie Yang, Yang Gu, Weihong Jiang, Yu Jiang, Sheng Yang* (2016) CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnology Journal. 11(7):961-72

48、Chen Yang, Xiang Gao, Yu Jiang, Bingbing Sun, Fang Gao, Sheng Yang*（2016）Synergy between methylerythritol phosphate pathway and mevalonate pathway for isoprene production in Escherichia coli. Metabolic Engineering, 2016, 37:79–91

49、Xiaoshu Xu, Jun Chen, Qingzhuo Wang, Chunlan Duan, Yan Li, Renxiao Wang,Sheng Yang*(2016) Mutagenesis of Key Residues in the Binding Center of l-Aspartate-b-Semialdehyde Dehydrogenase from Escherichia coli Enhances Utilization of the Cofactor NAD(H), ChemBioChem.17:56-64

50、Yu Jiang, Biao Chen, Chunlan Duan, Bingbing Sun, Junjie Yang, and Sheng Yang* (2015)Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl. Environ. Microbiol. 81:2506-14  ESI高被引

51、Ning Zhang, Lijun Shao, Yu Jiang, Yang Gu, Qi Li, Jinle Liu, Weihong Jiang, Sheng Yang* (2015) I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Journal Microbial Methods. 108:49-60

52、Yu Jiang, Jinle Liu, Yunliu Yang, Weihong Jiang, Sheng Yang* (2014) Current status and prospects of industrial bio-production of n-butanol in China, Biotechnology Advances. 33：1493-1501

53、Junjie Yang, Bingbing Sun, He Huang, Yu Jiang, Liuyang Diao, Biao Chen, Chongmao Xua,Xin Wang, Jinle Liu, Weihong Jiang, Sheng Yang* (2014) High-efficiency Scarless Genetic Modification in Escherichia coli Using lambda-Red Recombination and I-SceI Cleavage. Appl. Environ. Microbiol. 80(13):3826-34

54、Rongsheng Tao, Yu Jiang, Fuyun Zhu, Sheng Yang* (2014) A one-pot system for production ofL-2-aminobutyric acid from L-threonine by L-threonine deaminase and a NADH regeneration system based on L-leucine dehydrogenase and formate dehydrogenase. Biotechnology Letters 36(4):835-41

55、Liuyang Diao, Yingmiao Liu, Fenghui Qian, Junjie Yang, Yu Jiang, Sheng Yang* (2013）Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiaeby rational design and adaptive evolution. BMC Biotechnology 13:110

56、Zhilin Li, Han Xiao, Weihong Jiang, Yu Jiang*, Sheng Yang* (2013) Improvement of butanol production from xylose mother liquor by engineering xylose metabolic pathway in Clostridium acetobutylicum EA 2018. Applied Biochemistry and Biotechnology. 171(3):555-68

57、Han Xiao, Zhilin Li, Yu Jiang, Yunliu Yang, Weihong Jiang, Yang Gu*, Sheng Yang* (2012) Metabolic engineering of D-xylose pathway in Clostridium beijerinckii to optimize solvent production from xylose mother liquid. Metabolic Engineering 14: 565-578

58、Han Xiao, Yang Gu, Yuanyuan Ning, Yunliu Yang, W.J. Mitchell, Weihong Jiang*, Sheng Yang* (2011) Confirmation and elimination of xylose-metabolic bottlenecks in glucose-PTS-deficient Clostridium acetobutylicum to realize simultaneous utilization of glucose, xylose and arabinose. Appl. Environ. Microbiol 77:7886-7895

59、Zhoutong Sun, Yuanyuan Ning, Lixia Liu, Yingmiao Liu, Bingbing Sun, Weihong Jiang, Chen Yang and Sheng Yang* (2011) Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microbial Cell Factories. 10:71 Highly Accessed

60、Li Zhu, Rongsheng Tao, Yi Wang, Yu Jiang, Xin Lin, Yunliu Yang, Huabao Zheng, Weihong Jiang, Sheng Yang* (2011) Removal of L-alanine from the production of L-2-aminobutyric acid by introduction of alanine racemase and D-amino acid oxidase. Appl Microbiol Biotechnol. 90:903-910

61、Jian LI, Jingbo Zhao, Meng Zhao, Yunliu Yang, Weihong Jiang, Sheng Yang* (2010)Screening and Characterization of Butanol Tolerant Microorganisms. Letters in Applied Microbiology 50: 373-9

62、Yu Jiang, Chongmao Xu, Feng Dong, Yunliu Yang, Weihong Jiang*, Sheng Yang* (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metabolic Engineering 11: 284-91

63、Junjie Yang, Weihong Jiang, Sheng Yang* (2009). mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Research. 9(4): 600-9

64、Hong Yu, Jian Li, Dalong Zhang, Yunliu Yang, Weihong Jiang, Sheng Yang* (2009). Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR. Appl. Microbiol. Biotechnol. 82(2): 279-85

65、Lijun Shao, Shiyuan Hu, Yi Yang, Yang Gu, Jun Chen, Yunliu Yang, Weihong Jiang*, Sheng Yang* (2007) Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Research. 17: 963-5

杨晟研究员获上海市2015年度科技进步奖一等奖

文汇报：真正的科研人员应该有点阿Q精神

杨晟研究员被北京化工大学聘任为兼职教授

杨晟研究员荣获“诺维信创新奖”

文汇报：依托基础研究、转化平台、体制保障“三板斧”接通科研与产业

人民日报：让经济与科技不再两张皮

解放日报：没啥论文为企业创收却上亿

经济日报：打通创新价值链

光明日报:“湖州中心”成为 成果转化重要平台

科技日报：湖州显现“中科院平台效应”

浙江日报：借力国家队搭建新平台-湖州着力推进院地合作

钱江晚报：湖州生物医药产业渐成气候

湖州日报：中央媒体集中采访 中科院湖州应用技术 研究与产业化中心 自主创新平台建设

长春大成集团与中科院上海生科院工业生物中心产学研结合成果斐然

杨晟研究组建立高效的谷氨酸棒杆菌CRISPR-Cpf1基因组编辑系统

杨晟研究组建立偏利共生人工双梭菌系统用于转化玉米芯产溶剂