Cell reprogramming (cellular reprogramming) refers to the process of reversion of cells to omnipotent or pluripotent state under specific conditions, or the formation of pluripotent stem cell lines, or further development into a new individual. Nuclear transfer and induced pluripotent stem cell (iPSC) technology are two main reprogramming methods. However, these two technologies are still immature. There are some problems such as low efficiency, poor reprogramming ability and poor reprogramming mechanism.

2012年，研究组建立了来自小鼠精子的孤雄单倍体胚胎干细胞，并证明这些细胞能够代替精子使卵母细胞“受精”产生半克隆小鼠（称为“半克隆”技术）。然而，半克隆技术不成熟，半克隆小鼠出生率低（4%左右），而且有一半的半克隆小鼠存在发育缺陷问题。2015年，研究组获得了能稳定高效产生半克隆小鼠的“类精子细胞”的单倍体干细胞（出生效率达到20%左右），并证明这些细胞能携带CRISPR-Cas9文库一步获得大量携带不同突变的小鼠，为实现小鼠个体水平的遗传筛选奠定了基础：

In 2012, the research team established the haploid embryonic stem cells from spermatozoa in mice and proved that these cells could replace sperm to make oocytes "fertilized" to produce semi cloned mice (called "semi cloned"). However, the semi cloning technique is not mature, the birth rate of half cloned mice is low (about 4%), and half of the half cloned mice have developmental defects. In 2015, the research team obtained the haploid stem cells (about 20%) of "spermatozoa", which could produce semoncloned mice, which could produce a semoncloned mouse, and proved that these cells could carry a large number of mice carrying different mutations with the CRISPR-Cas9 library, which laid the foundation for the genetic screening of the individual level of mice.

本实验室开展以下几方面的研究：

The laboratory studies the following aspects:

完善核移植及iPSC技术诱导细胞重编程的方法，提高重编程的效率；

Objective to improve the method of cell reprogramming induced by nuclear transfer and iPSC technology, and to improve the efficiency of reprogramming.

1. 探讨重编程细胞发育缺陷相关的分子机制，提高重编程细胞的质量；

1. to explore the molecular mechanism of reprogramming cell development defects and improve the quality of reprogramming cells.

2. 探讨半克隆小鼠发育缺陷的相关分子机制，揭示与胚胎发育相关的重要机制；

2. to explore the molecular mechanisms of developmental defects in semi clonal mice, and to reveal important mechanisms related to embryonic development.

3. 结合“类精子细胞”的单倍体干细胞与CRISPR-Cas9技术，开展新的基因编辑应用研究;

3. combining the haploid stem cells and CRISPR-Cas9 technology of "spermatozoa like cells", we will develop new gene editing applications.

4. 开展CRISPR-Cas9技术介导的基因治疗研究。

4. carry out CRISPR-Cas9 mediated gene therapy research.

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​071008- developmental biology

071009- cell biology

The study of somatic cell reprogramming

体细胞核移植

nuclear transfer of somatic cell

诱导多能干细胞

Induced pluripotent stem cells

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1999-09--2002-06 CAS animal Institute

1993-09--1996-06 扬州大学 硕士

1993-09--1996-06 master of Yangzhou University

1989-09--1993-06 江西农业大学 学士

1989-09--1993-06 Bachelor of Jiangxi Agricultural University

2007-08~ now, research fellow, Institute of Biochemistry and cell biology, Shanghai Academy of life sciences, Chinese Academy of Sciences

2002-08~2007-07,洛克菲勒大学, 博士后研究

2002-08~2007-07, Rockefeller University, postdoctoral research

1997-04~1999-08,中科院动物所, 客座研究

1997-04~1999-08, animal Institute of Chinese Academy of Sciences, research on visitors

1996-07~1999-08,扬州大学, 助教，讲师

1996-07~1999-08, Yangzhou University, teaching assistantship, lecturer

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2015-11-01-2019-10-31,中国细胞生物学报, 编委

2015-11-01-2019-10-31, Chinese Journal of cell biology, editorial board

2015-10-01-2020-09-30,国家人口和计划生育委员会计划生育药具重点实验室, 学术委员会委员

2015-10-01-2020-09-30, member of the National Key Laboratory of family planning medicine and the academic committee of the state population and Family Planning Commission

2015-08-01-2018-07-31,中国细胞生物学会, 副秘书长

2015-08-01-2018-07-31, Deputy Secretary General of China Society of cell biology

2014-11-01-2018-10-31,中国动物学会细胞与分子显微技术学分会, 副理事长

2014-11-01-2018-10-31, vice president of the society of cell and molecular microscopy, Zoological Society of China

2014-11-01-2019-10-31,Journal of Biological chemistry, 编委

2014-11-01-2019-10-31, Journal of Biological chemistry, editorial board

（1）Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy., Cell Res, 2017,Communication author

（2）CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells., Methods Mol Biol, 2017,Communication author

（3）Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene-editing., J Genet Genomics, 2017,Communication author

（4）Efficient generation of gene-modified mice by haploid embryonic stem cell-mediated semi-cloned technology, Methods Mol Biol, 2017,Communication author

（5）An intermediate cell state allows rerouting of cell fate., J Biol Chem, 2017,Communication author
（6）CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development, Cell Res, 2017,Communication author

（7）Parthenogenetic haploid embryonic stem cells efficiently support mouse generation by oocyte injection, Cell Res, 2016,Communication author

（8）Generation and application of mammalian haploid embryonic stem cells, J Internal Med, 2016,Communication author

（9）Spermatogenic cell-specific gene mutation in mice via CRISPR-Cas9, J Genetic Genome, 2016,Communication author
（10）Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus, Cell Res, 2016,Communication author

（11）Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells, Cell Res, 2015,Communication author

（12）CRISPR-Cas9-mediated genetic screening in mice with haploid embryonic stem cells carrying a guide RNA library, Cell Stem Cell, 2015,Communication author

（13）Active and passive demethylation of male and female pronuclear DNA in the mammalian zygote, Cell Stem Cell , 2014,Communication author
（14）The roles of testicular c-kit positive cells in de novo morphogenesis of testis, Scientific Reports , 2014,Communication author
（15）Correction of a genetic disease in mouse via use of CRISPR-Cas9, Cell Stem Cell , 2013,Communication author
（16）Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes, Cell Research , 2013,Communication author
（17）Mice cloned from white adipose tissue-derived cells, J Mol Cell Biol , 2013,Communication author

（18）Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrasted by tetraploid complementation, Cell Research , 2013,Communication author

（19）Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells, Cell , 2012,Communication author

（20）Different developmental potential of pluripotent stem cells generated by different reprogramming strategies, J Mol Cell Biol, 2011, Communication author
（21）Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer., Cell Stem Cell, 2011,Communication author

（22）Mice generated after round spermatid injection into haploid two-cell blastomeres., Cell Res, 2011,Communication author
（23）The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes, Nature , 2011,Communication author
（24）Different developmental potential of pluripotent stem cells generated by different reprogramming strategies, J Mol Cell Biol, 2011,Communication author

（25）Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer, Cell Stem Cell , 2011,Communication author
（26）Mice generated after round spermatid injection into haploid two-cell blastomeres, Cell Research , 2011,Communication author
（27）High-efficiency somatic reprogramming induced by intact MII oocytes., Cell Res, 2010,Communication author（28）Nuclear transfer-mediated rescue of the nuclear genome of nonviable mouse cells frozen without cryoprotectant., Biol Reprod, 2008,First authors

（29）Mice cloned from skin cells. , Proc Natl Aca Sci USA, 2007,First authors
（30）Non-equivalence of cloned and clonal mice., Curr Biol, 2005,First authors
（31）Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons., Nature, 2004,First authors

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​ establishment and application of haploid haploid embryonic stem cells, invention, 2015, first author, patent No.: ZL201310103236.3

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(1) the biological basis of the key links of early embryo development, presided over, national level, 2007-07--2011-12

（ 2 ） 诱导多能干细胞（iPS)的重编程机制, 参与, 国家级, 2009-07--2013-12

(2) reprogramming mechanism of induced pluripotent stem cells (iPS), participation, national level, 2009-07--2013-12

（ 3 ） 细胞谱系的建立与发育调控, 参与, 国家级, 2011-01--2015-12

(3) establishment and development regulation of cell lineage, participation, national level, 2011-01--2015-12

（ 4 ） 核移植诱导体细胞重编程中供核细胞分化状态的影响, 主持, 国家级, 2009-07--2011-12

(4) the effect of nuclear transfer on the differentiation of donor cells in reprogramming of somatic cells, presided over, national level, 2009-07--2011-12

（ 5 ） 诱导多能干细胞（iPS）的新方法、分子机制及其应用研究, 参与, 省级, 2009-07--2011-12

(5) new method, molecular mechanism and Application Research of inducing pluripotent stem cells (iPS), participation, provincial level, 2009-07--2011-12

（ 6 ） 单倍体干细胞的建立、维持和应用, 主持, 国家级, 2014-01--2018-12

(6) the establishment, maintenance and application of haploid stem cells, presided over, national level, 2014-01--2018-12

（ 7 ） 细胞重编程与胚胎发育, 主持, 国家级, 2013-01--2016-12

(7) cell reprogramming and embryo development, hosting, national level, 2013-01--2016-12

（ 8 ） 克隆和半克隆胚胎发育的表观遗传调控研究, 主持, 国家级, 2014-01--2016-12

(8) epigenetic regulation of cloned and semi cloned embryos, host, national level, 2014-01--2016-12

（ 9 ） 孤雄单倍体胚胎干细胞携带CRISPR-Cas9文库用于筛选胚胎发育关键因子的研究, 主持, 国家级, 2016-01--2020-12

(9) haploid haploid embryonic stem cells carry CRISPR-Cas9 library for screening key developmental factors of embryo development, presided over, national level, 2016-01--2020-12

（ 10 ） 利用核酸酶靶向修饰，针对镰刀型贫血症、地中海贫血和艾滋病的新型治疗方法研究, 参与, 国家级, 2015-01--2017-12

(10) use nuclease targeting modification to study new therapies for sickle anemia, thalassemia and AIDS, participation, national level, 2015-01--2017-12

（ 11 ） 半克隆技术介导肌强直性营养不良小鼠模型的建立, 主持, 国家级, 2017-01--2019-12

(11) semi cloning technology mediated the establishment of a mouse model of myotonic dystrophy, presided over, national level, 2017-01--2019-12

（ 12 ） 儿童智力发育障碍研究资源库, 参与, 省级, 2016-12--2019-11

(12) children's mental retardation research resource pool, participation, provincial level, 2016-12--2019-11

（ 13 ） 细胞分裂方式的可塑性调控, 主持, 部委级, 2016-07--2020-12

(13) plasticity regulation of cell division mode, presided over, ministerial level, 2016-07--2020-12

（ 14 ） 利用“人造精子”介导半克隆技术研究早期胚胎发育中的表观遗传调控, 主持, 部委级, 2017-05--2022-04

(14) the use of "artificial sperm" mediated semi cloning technology to study epigenetic regulation in early embryonic development, presided over, ministry level, 2017-05--2022-04

（ 15 ） 基于“人造精子”技术的复杂疾病建模研究, 主持, 省级, 2017-09--2020-08

(15) modeling of complex diseases based on "artificial spermatozoa" technology, chaired, provincial, 2017-09--2020-08

（ 16 ） 基于“人造精子”技术的重要基因标签研究, 主持, 省级, 2017-12--2019-06

(16) an important gene labelling study based on "artificial sperm" technology, chaired, provincial, 2017-12--2019-06

（ 17 ） 利用半克隆技术筛选参与早期胚胎发育的关键雌性印记基因, 主持, 国家级, 2018-01--2022-12

(17) using semi cloning technology to screen key female imprinting genes involved in early embryo development, presided at national level, 2018-01--2022-12

（ 18 ） 精子发生过程中减数分裂启动和发展的分, 参与, 省级, 2018-01--2020-12

(18) the initiation and development of meiosis in the process of spermatogenesis, participation, provincial level, 2018-01--2020-12

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（1）Culturable sperm” for efficient generation of mice via injection into 2016-11-01
（2）When haploid embryonic stem cells met CRISPR-Cas9—“Artificial 2016-10-06
（3）When haploid cells met CRISPR-Cas9 2015-12-01
（4）Genome Editing in Male Germ Stem Cells 2015-05-31
（5）Generation of Mammalian Haploid Embryonic Stem Cells 2014-10-14
（6）Generation of Mammalian Haploid Embryonic Stem Cells 2014-09-01
（7）Generation of Mammalian Haploid Embryonic Stem Cells 2013-09-18
（8）Generation of Mammalian Haploid Embryonic Stem Cells 2013-06-12
（9）Cellular Reprogramming and Embryonic Development 2012-12-18
（10）Generation of androgenetic haploid embryonic stem cells 2012-12-04
（11）Generation of androgenetic haploid embryonic stem cells 2012-10-15
（12）Generation of androgenetic haploid embryonic stem cells 2012-02-25

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Instructing students

蒋婧  博士研究生  071008-发育生物学

Dr. Jiang Jing's postgraduate 071008- developmental biology

吕纹简  博士研究生  071008-发育生物学

Dr. Lv Wenjian's postgraduate 071008- developmental biology

杨辉  博士研究生  071008-发育生物学

Dr. Yang Hui's postgraduate 071008- developmental biology

张满  博士研究生  071008-发育生物学

Dr. Zhang Man's postgraduate 071008- developmental biology

施霖宇  博士研究生  071008-发育生物学

Dr. Shi Linyu's postgraduate 071008- developmental biology

汪凌波  博士研究生  071008-发育生物学

Dr. Wang Lingbo's postgraduate 071008- developmental biology

梁丹  博士研究生  071008-发育生物学

Dr. Liang Dan's postgraduate 071008- developmental biology

尹奇  博士研究生  071008-发育生物学

Dr. Yin Qi's postgraduate 071008- developmental biology

钟翠青  博士研究生  071008-发育生物学

Dr. Zhong Cuiqing's postgraduate 071008- developmental biology

现指导学生

Now guide students

谢振飞  博士研究生  071008-发育生物学

Dr. Xie Zhenfei's postgraduate 071008- developmental biology

王修坤  博士研究生  071008-发育生物学

Dr. Wang Xiukun's postgraduate 071008- developmental biology

廖娇阳  博士研究生  071008-发育生物学

Dr. Liao Jiaoyang's postgraduate 071008- developmental biology

王英华  博士研究生  071008-发育生物学

Dr. Wang Yinghua's postgraduate 071008- developmental biology

白梅竹  博士研究生  071008-发育生物学

Dr. Bai Meizhu's postgraduate 071008- developmental biology

孙展飞  硕士研究生  071008-发育生物学

The developmental biology of 071008- graduate student of Sun Zhanfei

张红玲  博士研究生  071008-发育生物学

Dr. Zhang Hongling's postgraduate 071008- developmental biology

杨苏明  博士研究生  071008-发育生物学

Dr. Yang Suming's postgraduate 071008- developmental biology

瞿超  博士研究生  071008-发育生物学

Dr. Qu Chao's postgraduate 071008- developmental biology

张晓宇  硕士研究生  071008-发育生物学

The developmental biology of 071008- graduate student of Zhang Xiaoyu

李庆  博士研究生  071008-发育生物学

Dr. Li Qing's postgraduate 071008- developmental biology

马永健  博士研究生  071008-发育生物学

Dr. Ma Yongjian's postgraduate 071008- developmental biology

陈建新  硕士研究生  071008-发育生物学

The developmental biology of 071008- graduate student of Chen Jianxin

(1) 2014-2015 Shanghai advanced voluntary service group, provincial level, 2016

（2） 干细胞社会公益奖, 国家级, 2016

(2) social public welfare award for stem cells, national level, 2016

（3） 树兰医学青年奖, 国家级, 2016

(3) the orchid medical Youth Award, national level, 2016

（4） 光华工程科技奖, 国家级, 2016

(4) Guanghua Engineering Science and technology award, national level, 2016

（5） 上海市五一劳动奖章, 省级, 2016

(5) the labor medal of the May 1 in Shanghai, provincial, 2016

（6） 国家百千万人才工程, 国家级, 2015

(6) National 10 million talents project, national level, 2015

（7） 2013-2014年度上海市科技系统先进工作者, 省级, 2015

(7) 2013-2014 advanced workers in Shanghai science and technology system, provincial level, 2015

（8） 中国细胞生物学会“R&D Systems杰出成就奖”, 其他, 2015

(8) R&D Systems outstanding achievement award of China Society of cell biology, others, 2015

（9） 何梁何利科学与技术创新奖, 其他, 2014

(9) ho Liang ho ho for science and Technology Innovation Award, others, 2014

（10） 谈家桢生命科学创新奖, 其他, 2014

(10) Tan Jiazhen's life science innovation award, others, 2014

（11） 中青年科技创新领军人才, 部委级, 2014

(11) young and middle-aged leaders in science and technology innovation, ministries and commissions, 2014

（12） 优秀研究生指导教师, 院级, 2013

(12) excellent postgraduate tutors, hospital level, 2013

（13） 普洛麦格创新奖, 其他, 2013

(13) prore prize for innovation, others, 2013

（14） 上海市“青年科技杰出贡献奖”, 省级, 2013

(14) Shanghai outstanding youth science and technology award, provincial, 2013

（15） 优秀博士导师奖, 院级, 2012

(15) the award of excellent doctorate tutor, hospital level, 2012

（16） 证实单倍体孤雄干细胞具有可替代精子和快速传递基因修饰的能力, 其他, 2012

(16) it is confirmed that haploid stem cells have the ability to replace sperm and express gene transfer rapidly. 2012,

（17） A-IMBN Research Young Investigators Award, 其他, 2012

(17) A-IMBN Research Young Investigators Award, others, 2012

（18） 揭示Tet双加氧酶在哺乳动物表现遗传调控中的重要作用, 其他, 2011

(18) to reveal the important role of Tet double oxygenase in the regulation of mammalian expression, others, 2011

（19） 哺乳动物有性和无性生殖的实验胚胎学研究, 二等奖, 国家级, 2006

(19) experimental embryology of sexual and asexual mammals, two prize, national level, 2006

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