（1）开发以生物质谱为核心的多种蛋白质组学的分析技术和方法；
（2）发展适用于生物样品和临床样品的新型糖组学的分析技术和方法；
（3）在蛋白质组学和糖组学分析技术的基础上，主要开展针对蛋白质及其翻译后修饰的表达调控及其在人体功能和临床疾病中的作用机制的研究。

   

071010-生物化学与分子生物学

应用生物质谱等分析技术研究糖组学及蛋白质组学的基础和临床问题
生物质谱技术
转化医学

2005-10--2011-06 美国约翰霍普金斯大学医学院 博士后
2000-09--2004-07 美国马里兰大学 博士
1994-09--1998-07 北京大学医学部（原北京医科大学） 学士

博士研究生

博士

   

2011-07--今 中国科学院生物物理研究所 研究员
2005-10--2011-06 美国约翰霍普金斯大学医学院 助理研究员/博士后
2004-01--2005-08 美国Calibrant生物技术公司 项目主管
2000-08--2004-05 美国马里兰大学化学与分析化学系 研究助理

   

（1） Young Investigator Travel Award，一等奖，其他级，2010
（2） Outstanding Poster Award，一等奖，其他级，2009
（3） Young Investigator Travel Award，一等奖，其他级，2009

[1] 罗丹, 孙青, 卞铮, 张楚凡, 李岩. 一种用于人群调查的电子签章设备. CN: CN218032382U, 2022-12-13.

[2] 李岩, 罗丹, 孙青, 卞铮, 张楚凡. 一种用于人群调查的电子签章设备. 202222477244.1, 2022-05-20.

[3] 梁锴, 李岩, 赵克力, 孙青. 一种血液样本质量评估方法. CN: CN112745374A, 2021-05-04.

[4] 孙世伟, 卜东波, 杨飞, 王耀军, 李岩, 黄纯翠, 陈润生, 高枫, 刘亚名. 一种糖结构鉴定方法及糖结构鉴定装置. CN: CN105758928B, 2019-04-30.

[5] 孙世伟, 王耀君, 卜东波, 李岩, 黄纯翠, 刘亚名, 杨飞, 武红梅, 陈润生. 多级质谱生物大分子结构鉴定方法. CN: CN104965020B, 2017-07-21.

[6] 李岩, 张建中, 李倩倩, 赵倩倩, 肖迪. 一种脂多糖中寡糖的纯化及质谱鉴定方法. CN: CN106932258A, 2017-07-07.

[7] 何建国, 黄纯翠, 貊泽强, 余锦, 李岩, 王金舵, 林蔚然, 刘洋, 刘昊. 一种微流控芯片的封接装置与封接方法. CN: CN106219482A, 2016-12-14.

[8] 孙世伟, 卜东波, 杨飞, 王耀军, 李岩, 黄纯翠, 陈润生, 高枫, 刘亚名. 一种糖结构鉴定方法及糖结构鉴定装置. CN: CN105758928A, 2016-07-13.

[9] 孙世伟, 王耀君, 卜东波, 李岩, 黄纯翠, 刘亚名, 杨飞, 武红梅, 陈润生. 多级质谱生物大分子结构鉴定方法. CN: CN104965020A, 2015-10-07.

   

[1] Chuncui Huang, Yue Lu, Lin Kong, Zhendong Guo, Keli Zhao, Zheng Xiang, Xinyue Ma, Huanyu Gao, Yongfang Liu, Zhongmin Gao, Lijuan Xu, Wengang Chai, Yan Li, Yao Zhao. Human milk oligosaccharides in milk of mothers with term and preterm delivery at different lactation stage. CARBOHYDRATE POLYMERS[J]. 2023, 321: http://dx.doi.org/10.1016/j.carbpol.2023.121263.
[2] 马心悦, 黄纯翠, 赵耀, 李岩. 人乳寡糖的结构及其分离分析. 生物化学与生物物理进展[J]. 2023, [3] chuncui huang, meijie hou, jingyu yan, Wengang Chai, Yan Li, Shiwei Sun. GIPS-Mix for Accurate Identification of Isomeric Components in Glycan Mixtures Using Intelligent Group-Opting Strategy. Analytical Chemistry[J]. 2023, https://pubs.acs.org/doi/abs/10.1021/acs.analchem.2c02978.
[4] Liang Kai, Yan Li, Qianqian Li, Zhijing Song, keli zhao, Rong Su, Shengchun Huang, Xueyan Guo. Plasma Peptides Modulated by Protease in a Time-Dependent Manner as Effective Biomarkers for Preanalytical Quality Control. JOURNAL OF PROTEOME RESEARCH[J]. 2023, [5] Wang, Junyan, Zhao, Keli, Chen, Ling, Zhou, Jinyu, Sun, Qing, Chen, Junhui, Su, Rong, Li, Yan. Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging. ANALYTICAL BIOCHEMISTRY[J]. 2022, 652: http://dx.doi.org/10.1016/j.ab.2022.114770.
[6] Zhang Q. Q, 李岩. MALDI–TOF–MS for Rapid Screening and Typing of β-Globin Variant and β-Thalassemia through Direct Measurements of Intact Globin Chains. Clinical Chemistry[J]. 2022, [7] Zou, Wenjun, Huang, Chuncui, Sun, Qing, Zhao, Keli, Gao, Huanyu, Su, Rong, Li, Yan. A stepwise mutagenesis approach using histidine and acidic amino acid to engineer highly pH-dependent protein switches. 3 BIOTECH[J]. 2022, 12(1): http://dx.doi.org/10.1007/s13205-021-03079-x.
[8] Liu, Lu, Xie, Wenchun, Song, Zhijing, Wang, Tianyu, Li, Xuesong, Gao, Ying, Li, Yan, Zhang, Junqing, Guo, Xiaohui. Addition of 3-methoxytyramine or chromogranin A to plasma free metanephrines as the initial test for pheochromocytoma and paraganglioma: Which is the best diagnostic strategy. CLINICAL ENDOCRINOLOGY. 2021, [9] Huang, Chuncui, Tan, Zeshun, Zhao, Keli, Zou, Wenjun, Wang, Hui, Gao, Huanyu, Sun, Shiwei, Bu, Dongbo, Chai, Wengang, Li, Yan. The effect of N-glycosylation of SARS-CoV-2 spike protein on the virus interaction with the host cell ACE2 receptor. ISCIENCE[J]. 2021, 24(11): http://dx.doi.org/10.1016/j.isci.2021.103272.
[10] Wang, Junyan, Huang, Chuncui, Zhou, Jinyu, Zhao, Keli, Li, Yan. Causal link between immunoglobulin G glycosylation and cancer: A potential glycobiomarker for early tumor detection. CELLULAR IMMUNOLOGYnull. 2021, 361: http://dx.doi.org/10.1016/j.cellimm.2021.104282.
[11] Song, Zhijing, Gao, Huanyu, Xie, Wenchun, Sun, Qing, Liang, Kai, Li, Yan. Quantitative MALDI-MS assay of steroid hormones in plasma based on hydroxylamine derivatization. ANALYTICAL BIOCHEMISTRY[J]. 2021, 616: http://dx.doi.org/10.1016/j.ab.2020.114089.
[12] 孙青, 梁锴, 李岩. 血液样本质量的评估技术进展. 生物化学与生物物理进展[J]. 2021, 48(8): 938-946, http://lib.cqvip.com/Qikan/Article/Detail?id=7105583252.
[13] Sun Qing, Liang Kai, Li Yan. Quality Assessment for The Blood Samples. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2021, 48(8): 938-946, http://dx.doi.org/10.16476/j.pibb.2020.0375.
[14] Wang, Hui, Wang, Yu, Hou, Meijie, Zhang, Chunming, Wang, Yaojun, Guo, Zhendong, Bu, Dongbo, Li, Yan, Huang, Chuncui, Sun, Shiwei. HepParser: An Intelligent Software Program for Deciphering Low-Molecular-Weight Heparin Based on Mass Spectrometry. FRONTIERS IN CHEMISTRY[J]. 2021, 9: http://dx.doi.org/10.3389/fchem.2021.723149.
[15] Huang, Chuncui, Sun, Shiwei, Yan, Jingyu, Wang, Hui, Zhou, Jinyu, Gao, Huanyu, Xie, Wenchun, Li, Yan, Chai, Wengang. Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry. CARBOHYDRATE POLYMERS[J]. 2020, 229: http://dx.doi.org/10.1016/j.carbpol.2019.115528.
[16] 段云峰, 王升跃, 陈禹保, 杨瑞馥, 李后开, 朱怀球, 童贻刚, 杜文斌, 付钰, 胡松年, 王军, 辛玉华, 赵方庆, 鲍一明, 张雯, 李娟, 曾明, 牛海涛, 周欣, 李岩, 崔生辉, 袁静, 李俊桦, 王加义, 刘东来, 倪铭, 孙青, 邓晔, 朱宝利. 微生物组测序与分析专家共识. 生物工程学报[J]. 2020, 36(12): 2516-2524, http://lib.cqvip.com/Qikan/Article/Detail?id=7103610501.
[17] Zhou, Jinyu, Gao, Huanyu, Xie, Wenchun, Li, Yan. Bovine serum albumin affects N-glycoforms of murine IgG monoclonal antibody purified from hybridoma supernatants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2020, 104(4): 1583-1594, https://www.webofscience.com/wos/woscc/full-record/WOS:000519153800016.
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[19] Wang, Hui, Zhang, Jingwei, Dong, Junchuan, Hou, Meijie, Pan, Weiyi, Bu, Dongbo, Zhou, Jinyu, Zhang, Qi, Wang, Yaojun, Zhao, Keli, Li, Yan, Huang, Chuncui, Sun, Shiwei. Identification of glycan branching patterns using multistage mass spectrometry with spectra tree analysis. JOURNAL OF PROTEOMICS[J]. 2020, 217: http://dx.doi.org/10.1016/j.jprot.2020.103649.
[20] Hou, Meijie, Wang, Yu, Wang, Hui, Wang, Yaojun, Zhao, Keli, Li, Yan, Bu, Dongbo, Huang, Chuncui, Sun, Shiwei. Toward automated identification of glycan mixtures using multistage mass spectrometry. GLYCOBIOLOGY[J]. 2020, 30(12): 1061-1061, https://www.webofscience.com/wos/woscc/full-record/WOS:000606577100089.
[21] Huang, Chuncui, Wang, Hui, Bu, Dongbo, Zhou, Jinyu, Dong, Junchuan, Zhang, Jingwei, Gao, Huanyu, Wang, Yaojun, Chai, Wengang, Sun, Shiwei, Li, Yan. Multistage mass spectrometry with intelligent precursor selection for N-glycan branching pattern analysis. CARBOHYDRATE POLYMERS[J]. 2020, 237: http://dx.doi.org/10.1016/j.carbpol.2020.116122.
[22] Huang, Chuncui, Yan, Jingyu, Zhan, Lingpeng, Zhao, Min, Zhou, Jinyu, Gao, Huanyu, Xie, Wenchun, Li, Yan, Chai, Wengang. Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation. ANALYTICA CHIMICA ACTA[J]. 2019, 1071: 25-35, http://cas-ir.dicp.ac.cn/handle/321008/171871.
[23] Jinyu Zhou, Huanyu Gao, Wenchun Xie, Yan Li. FcγR-binding affinity of monoclonal murine IgG1s carrying different N-linked Fc oligosaccharides. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2019, 520(1): 8-13, http://dx.doi.org/10.1016/j.bbrc.2019.09.068.
[24] Wang, Yaojun, Bu, Dongbo, Huang, Chuncui, Wang, Hui, Zhou, Jinyu, Dong, Junchuan, Pan, Weiyi, Zhang, Jingwei, Zhang, Qi, Li, Yan, Sun, Shiwei. Best-first search guided multistage mass spectrometry-based glycan identification. BIOINFORMATICS[J]. 2019, 35(17): 2991-2997, https://www.webofscience.com/wos/woscc/full-record/WOS:000487323400014.
[25] Ju, Fusong, Zhang, Jingwei, Bu, Dongbo, Li, Yan, Zhou, Jinyu, Wang, Hui, Wang, Yaojun, Huang, Chuncui, Sun, Shiwei. De novo glycan structural identification from mass spectra using tree merging strategy. COMPUTATIONAL BIOLOGY AND CHEMISTRY[J]. 2019, 80: 217-224, http://dx.doi.org/10.1016/j.compbiolchem.2019.03.015.
[26] Zhang WeiWei, Gao HuanYu, Sun Qing, Liang Kai, Xie WenChun, Li Yan. LC-MS/MS Studies on Sample Quality Control of Fabry Disease. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2019, 46(8): 823-834, https://www.webofscience.com/wos/woscc/full-record/WOS:000482545900009.
[27] Sun, Dehui, Hu, Fanlei, Gao, Huanyu, Song, Zhijing, Xie, Wenchun, Wang, Ping, Shi, Lianjie, Wang, Ke, Li, Yan, Huang, Chuncui, Li, Zhanguo. Distribution of abnormal IgG glycosylation patterns from rheumatoid arthritis and osteoarthritis patients by MALDI-TOF-MSn. ANALYST[J]. 2019, 144(6): 2042-2051, [28] 张卫威, 高环宇, 孙青, 梁锴, 谢雯春, 李岩. 液相色谱串联质谱法检测法布雷病样本的质量控制研究（英文）. 生物化学与生物物理进展[J]. 2019, 823-834, http://lib.cqvip.com/Qikan/Article/Detail?id=83728387504849574856484857.
[29] Zhou, Jinyu, Gao, Huanyu, Xie, Wenchun, Li, Yan. Fc gamma R-binding affinity of monoclonal murine IgG1 s carrying different N-linked Fc oligosaccharides. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2019, 520(1): 8-13, https://www.webofscience.com/wos/woscc/full-record/WOS:000506410000002.
[30] 袁凤喜, 薛雄燕, 陈彩凤, 赖月红, 梁泳仪, 李岩, 谢雯春, 杨绍杰, 吴燕杰. 溶血对液相色谱串联质谱检测血浆中儿茶酚胺及其代谢产物的影响. 检验医学[J]. 2018, 33(12): 1103-1107, http://lib.cqvip.com/Qikan/Article/Detail?id=7000993949.
[31] Liang, Kai, Gao, Huanyu, Gu, Yajun, Yang, Shaojie, Zhang, Jinling, Li, Jiejie, Wang, Yilong, Wang, Yongjun, Li, Yan. Graphene oxide aggregate-assisted LDI-MS for the direct analysis of triacylglycerol in complex biological samples. ANALYTICA CHIMICA ACTA[J]. 2018, 1035: 108-118, https://www.webofscience.com/wos/woscc/full-record/WOS:000444918000012.
[32] Wang, Xiaoning, Deng, Zaian, Huang, Chuncui, Zhu, Tong, Lou, Jiatao, Wang, Lin, Li, Yan. Differential N-glycan patterns identified in lung adenocarcinoma by N-glycan profiling of formalin-fixed paraffin-embedded (FFPE) tissue sections. JOURNAL OF PROTEOMICS[J]. 2018, 172: 1-10, http://dx.doi.org/10.1016/j.jprot.2017.11.010.
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[34] Liu, Chang, Lyon, Christopher J, Bu, Yang, Deng, Zaian, Walters, Elisabetta, Li, Yan, Zhang, Liqun, Hesseling, Anneke C, Graviss, Edward A, Hu, Ye. Clinical Evaluation of a Blood Assay to Diagnose Paucibacillary Tuberculosis via Bacterial Antigens. CLINICAL CHEMISTRY[J]. 2018, 64(5): 791-800, https://www.webofscience.com/wos/woscc/full-record/WOS:000448290000008.
[35] Li Yan. Toward Automated Identification of Glycan Branching Patterns Using Multistage Mass Spectrometry with Intelligent Precursor Selection. Analytical Chemistry. 2018, [36] Zhao, Qianqian, Zhan, Tiancheng, Deng, Zaian, Li, Qianqian, Liu, Yaming, Yang, Shaojie, Ji, Dengbo, Li, Yan. Glycan analysis of colorectal cancer samples reveals stage-dependent changes in CEA glycosylation patterns. CLINICAL PROTEOMICS[J]. 2018, 15(1): https://doaj.org/article/3cee7744ba744420bc99eb559e294ee2.
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[38] 李炜煊, 李倩倩, 杨绍杰, 李岩. 液相色谱-串联质谱联用法检测人体类固醇激素的研究进展. 中华检验医学杂志[J]. 2017, 40(10): 816-822, https://d.wanfangdata.com.cn/periodical/zhyxjy201710016.
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[47] Huang, Chuncui, Liu, Yaming, Wu, Hongmei, Sun, Dehui, Li, Yan. Characterization of IgG glycosylation in rheumatoid arthritis patients by MALDI-TOF-MSn and capillary electrophoresis. ANALYTICAL AND BIOANALYTICAL CHEMISTRY[J]. 2017, 409(15): 3731-3739, https://www.webofscience.com/wos/woscc/full-record/WOS:000401407900004.
[48] Wang YaoJun, Huang ChunCui, Gao Feng, Zhang JingWei, Li Yan, Bu DongBo, Sun ShiWei. Survey on Algorithms for Tandem Mass Spectrometry Oligosaccharide Identification. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2017, 44(10): 830-847, https://www.webofscience.com/wos/woscc/full-record/WOS:000413991500003.
[49] Zhou JinYu, Li QianQian, Huang ChunCui, Wu HongMei, Li Yan. Recent Advances in The Structural Analysis of Bacterial Lipopolysaccharide and Its Oligosaccharide Chains. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2017, 44(1): 49-57, https://www.webofscience.com/wos/woscc/full-record/WOS:000392575600007.
[50] Kai Liang, Fei Liu, Jia Fan, Dali Sun, Chang Liu, Christopher J Lyon, David W Bernard, Yan Li, Kenji Yokoi, Matthew H Katz, Eugene J Koay, Zhen Zhao, Ye Hu. Nanoplasmonic Quantification of Tumor-derived Extracellular Vesicles in Plasma Microsamples for Diagnosis and Treatment Monitoring. NATURE BIOMEDICAL ENGINEERING. 2017, 1: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543996/.
[51] Liang, Kai, Wu, Hongmei, Li, Yan. Immune-enrichment of insulin in bio-fluids on gold-nanoparticle decorated target plate and in situ detection by MALDI MS. CLINICAL PROTEOMICS[J]. 2017, 14(1): http://dx.doi.org/10.1186/s12014-017-9139-z.
[52] 李岩. 基于质谱技术的糖链结构解析研究进展. 生物化学与生物物理进展. 2017, [53] Liang, Kai, Wu, Hongmei, Hu, Tony Y, Li, Yan. Mesoporous silica chip: enabled peptide profiling as an effective platform for controlling bio-sample quality and optimizing handling procedure. CLINICAL PROTEOMICS[J]. 2016, 13(1): http://dx.doi.org/10.1186/s12014-016-9134-9.
[54] Huang, Chuncui, Zhan, Tiancheng, Liu, Yaming, Wu, Hongmei, Li, Yan. Characterization of IgG glycosylation in rheumatoid arthritis patients by MALDI-TOF-MSn and Capillary Electrophoresis. GLYCOBIOLOGYnull. 2016, 26(12): 1486-1487, https://www.webofscience.com/wos/woscc/full-record/WOS:000392935600277.
[55] Sun, Shiwei, Huang, Chuncui, Wang, Yaojun, Liu, Naming, Chai, Wengang, Yang, Fei, Zhang, Jingwei, Gao, Feng, Chen, Runsheng, Li, Yan, Bu, Dongbo. Towards automated identification of glycan branching patterns using multistage mass spectrometry with intelligent precursor selection. GLYCOBIOLOGYnull. 2016, 26(12): 1458-1458, https://www.webofscience.com/wos/woscc/full-record/WOS:000392935600208.
[56] 张自强, 李岩. 质谱技术在临床生化检测中的应用. 检验医学[J]. 2015, 30(5): 407-409, http://lib.cqvip.com/Qikan/Article/Detail?id=664857665.
[57] Li, Yan, Shah, Punit, De Marzo, Angelo M, Van Eyk, Jennifer E, Lo, Qianqian, Chan, Daniel W, Zhang, Hui. Identification of Glycoproteins Containing Specific Glycans Using a Lectin-Chemical Method. ANALYTICAL CHEMISTRY[J]. 2015, 87(9): 4683-4687, https://www.webofscience.com/wos/woscc/full-record/WOS:000354153600016.
[58] Shanshan Yuan, Qianqian Li, Yang Zhang, Chuncui Huang, Hongmei Wu, Yan Li, Yalei Liu, Nan Yu, Hong Zhang, Guizhi Lu, Yanming Gao, Ying Gao, Xiaohui Guo. Changes in Anti-Thyroglobulin IgG Glycosylation Patterns in Hashimotoʼs Thyroiditis Patients. THE JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM. 2015, 100(2): [59] Deng, Zaian, Li, Yaojun, Fan, Jia, Wang, Guohui, Li, Yan, Zhang, Yaou, Cai, Guoping, Shen, Haifa, Ferrari, Mauro, Hu, Tony Y. Circulating Peptidome to Indicate the Tumor-resident Proteolysis. SCIENTIFIC REPORTS[J]. 2015, 5: http://www.corc.org.cn/handle/1471x/2212512.
[60] 赵倩倩, 张自强, 李岩. 质谱技术在肿瘤蛋白质标志物研究中的应用与发展. 中华检验医学杂志[J]. 2015, 38(2): 82-84, https://d.wanfangdata.com.cn/periodical/zhyxjy201502004.
[61] Huang, Chuncui, Zhan, Tiancheng, Liu, Yaming, Li, Qianqian, Wu, Hongmei, Ji, Dengbo, Li, Yan. Glycomic profiling of carcinoembryonic antigen isolated from human tumor tissue. CLINICAL PROTEOMICS[J]. 2015, 12(1): http://dx.doi.org/10.1186/s12014-015-9088-3.
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（1） 人群样本库血液质量评估体系的建立与应用，主持，院级级，2015-07--2017-06
（2） 医学检测服务与技术研发，参与，院级级，2014-01--2015-12
（3） 代谢物组与非编码RNA的系统识别与鉴定关键技术研发，参与，国家级，2014-01--2016-12
（4） 中国人群前瞻性生物样本库建设，主持，院级级，2014-01--2017-12
（5） 胁迫环境中细胞代谢的调控机制，参与，国家级，2013-01--2017-12
（6） 可替代细菌血清学鉴定的高通量分子甄别技术的研发，参与，国家级，2013-01--2015-12
（7） 蛋白质糖基化修饰的高通量质谱检测技术，主持，国家级，2012-12--2016-12

   

北京肿瘤医院：结直肠癌分子标志物的发现；
国家纳米科学中心：糖组学二维分析芯片及配套仪器的开发；
中国科学院光电研究院、北京国科世纪激光技术有限公司：病理诊断新型仪器开发。

个人主页网站：http://www.ibp.cas.cn/ktzz/ktzz_L/201305/t20130506_3833253.html