应用液体核磁共振波谱技术研究生物大分子的结构、动力学性质和相互作用，研究方向包括：基因转录过程中蛋白质-DNA、蛋白质-蛋白质复合物的组装和调控机制；磷酸化修饰调控蛋白质构象变化和蛋白质-蛋白质间相互作用；致病基因突变诱发蛋白质结构和功能改变的机理等。

   

070302-分析化学
0703Z1-化学生物学
071010-生物化学与分子生物学

蛋白质结构与功能
液体核磁共振

2006-09--2014-06   武汉大学   理学博士
2001-09--2005-06   武汉大学   理学学士

研究生

博士

   

2020-12~现在, 中国科学院精密测量科学与技术创新研究院, 副研究员
2017-10~2020-11,中国科学院精密测量科学与技术创新研究院（原武汉物理与数学研究所）, 助理研究员
2015-07~2017-09,中国科学院武汉物理与数学研究所, 博士后

   

（ 1 ） 一种具有高特异性识别黄曲霉毒素B1的纳米抗体2018AFB-N11及其应用, 发明, 2020, 第 3 作者, 专利号: ZL201910129322.9
（ 2 ） 一种黄曲霉毒素B1纳米抗体的时间分辨荧光免疫层析试剂盒及其制备方法和应用, 发明, 2019, 第 4 作者, 专利号: 201910137204.2
（ 3 ） 一种杀菌摇床, 实用新型, 2018, 第 1 作者, 专利号: ZL201720743173.1

   

[1] Shan, Yubao, He, Xiaoling, Wang, Zi, Yue, Xiali, Zhu, Jiang, Yang, Yunhuang, Liu, Maili. Structural Investigations on the SH3b Domains of Clostridium perfringens Autolysin through NMR Spectroscopy and Structure Simulation Enlighten the Cell Wall Binding Function. MOLECULES[J]. 2021, 26(18): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470621/.
[2] Xiaoling He, Yao Nie, Heng Zhou, Rui Hu, Ying Li, Ting He, Jiang Zhu, Yunhuang Yang, Maili Liu. Structural Insight into the Binding of TGIF1 to SIN3A PAH2 Domain through a C-Terminal Amphipathic Helix. International Journal of Molecular Sciences[J]. 2021, 22(23): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657803/.
[3] Heng Zhou, Xiali Yue, Zi Wang, Shuangli Li, Jiang Zhu, Yunhuang Yang, Maili Liu. Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli. Protein Expression and Purification[J]. 2021, 180: http://dx.doi.org/10.1016/j.pep.2021.105821.
[4] Gong, Yixuan, Li, Shuangli, Li, Ying, Zhu, Jiang, Yang, Yunhuang, Liu, Maili. Mutation of leucine 20 causes a change of local conformation indirectly impairing the DNA binding of SP_0782 from Streptococcus pneumoniae. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2020, 524(1): 103-108, http://dx.doi.org/10.1016/j.bbrc.2020.01.057.
[5] Cai, Cong, Nie, Yao, Gong, Yixuan, Li, Shuangli, Ramelot, Theresa A, Kennedy, Michael A, Yue, Xiali, Zhu, Jiang, Liu, Maili, Yang, Yunhuang. Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2020, 88(1): 237-241, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901738/.
[6] Li, Shuangli, Lu, Guoliang, Fang, Xiang, Ramelot, Theresa A, Kennedy, Michael A, Zhou, Xin, Gong, Peng, Zhang, Xu, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Structural insight into the length-dependent binding of ssDNA by SP_0782 from Streptococcus pneumoniae, reveals a divergence in the DNA-binding interface of PC4-like proteins. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(1): 432-444, https://www.webofscience.com/wos/woscc/full-record/WOS:000524741700037.
[7] Zhu, Wenkai, Li, Ying, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity. JOURNAL OF PHYSICAL CHEMISTRY LETTERS[J]. 2019, 10(6): 1272-1278, https://pubs.acs.org/doi/10.1021/acs.jpclett.9b00052.
[8] Wang, Zi, Nie, Yao, Zhang, Kunxiao, Xu, Henghao, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Solution structure of SHIP2 SH2 domain and its interaction with a phosphotyrosine peptide from c-MET. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 656: 31-37, http://dx.doi.org/10.1016/j.abb.2018.08.012.
[9] Zhu, Jiang, Li, Shuangli, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Yang, Yunhuang. Structural insights into the impact of two holoprosencephaly-related mutations on human TGIF1 homeodomain. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 496(2): 575-581, http://dx.doi.org/10.1016/j.bbrc.2018.01.099.
[10] Li, Shuangli, Hu, Rui, Yao, Haijie, Long, Dong, Luo, Fan, Zhou, Xin, Zhang, Xu, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Characterization of the interaction interface and conformational dynamics of human TGIF1 homeodomain upon the binding of consensus DNA. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS[J]. 2018, 1866(10): 1021-1028, http://dx.doi.org/10.1016/j.bbapap.2018.07.005.
[11] Zhu, Jiang, Wang, Huapu, Ramelot, Theresa A, Kennedy, Michael A, Hu, Rui, Yue, Xiali, Liu, Maili, Yang, Yunhuang. Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2017, 85(5): 957-962, https://www.webofscience.com/wos/woscc/full-record/WOS:000399380400015.

   

（ 1 ） 神经内分泌调控蛋白INSM1锌指结构域的核磁共振结构和功能研究, 主持, 国家级, 2018-01--2020-12
（ 2 ） 核磁共振波谱研究转录抑制复合物Sin3A-TGIF1-Smad2组装的分子机制, 参与, 国家级, 2021-01--2024-12
（ 3 ） 细胞中原子分辨的功能分子构象变化、相互作用和微环境效应的测定, 主持, 国家级, 2020-01--2024-12
（ 4 ） 生物磁共振波谱分析中的原子标记和非标记方法研究, 主持, 国家级, 2018-01--2022-12
（ 5 ） 基于蛋白质液晶化定向排列芯片的RDC核磁样品制备新方法研究, 参与, 国家级, 2018-01--2020-12