李密，男，博士，研究员，博士生导师，国家自然科学基金委员会优秀青年科学基金项目获得者。2006年6月毕业于华中科技大学软件工程专业，获工学学士学位；2008年12月毕业于华中科技大学模式识别与智能系统专业，获工学硕士学位；2015年1月毕业于中国科学院沈阳自动化研究所模式识别与智能系统专业，获工学博士学位。2016年9月至2017年8月在美国俄亥俄州立大学(The Ohio State University)做访问学者，2020年10月至2021年3月在瑞士苏黎世联邦理工学院(ETH Zurich)做访问学者。2014年3月提前留所至今在中国科学院沈阳自动化研究所工作，历任助理研究员、副研究员、研究员，自2021年起担任博士生导师。

        从事微纳生物操控机器人学基础理论与应用研究工作，主要研究兴趣和方向包括面向生物医学应用的微纳机器人与自动化系统，基于原子力显微镜(AFM)的微纳生物操控与检测，以及微纳生物图像数据智能分析等，目前发表第一作者/通讯作者SCI期刊论文60余篇，论文发表在Nano Letters，Nano Research，Nanoscale，Langmuir，IEEE Trans汇刊等国内外知名刊物，在Elsevier/Springer出版独立著作英文学术专著2部(ISBN: 978-0-323-95360-3; ISBN: 978-981-10-6828-7)，主持国家自然科学基金项目4项(优青1项，面上2项，青年1项)，先后获得国家自然科学基金委员会优秀青年科学基金项目、中国科学院院长特别奖、中国科学院优秀博士学位论文、中国自动化学会优秀博士学位论文、德国施普林格优秀博士学位论文(Springer Theses)、IEEE高级会员(IEEE Senior Member)、辽宁省“兴辽英才计划”青年拔尖人才、辽宁省“百千万人才工程”千层次人选等学术荣誉奖励。

       每年招收博士研究生2名，硕士研究生1名，非常欢迎具有自动控制、人工智能、机械电子、电气工程、光学工程等相关学科专业背景的推免生和考研生/考博生报名加入攻读硕士/博士研究生学位，成绩优异者有出国学习深造机会，目前在读研究生中有多人次先后获得研究生国家奖学金、研究生学业奖学金、中国科学院大学三好学生等荣誉奖励，有意者可直接与我邮件 (E-mail: limi@sia.cn)联系。

   

081104-模式识别与智能系统
081102-检测技术与自动化装置
081101-控制理论与控制工程

微纳生物操控机器人系统
原子力显微镜生物探测
微纳生物图像智能分析

2009-03--2015-01   中国科学院沈阳自动化研究所   获得工学博士学位
2006-09--2008-12   华中科技大学   获得工学硕士学位
2002-09--2006-06   华中科技大学   获得工学学士学位

   

2020-10~2021-03,ETH Zurich, Switzerland, 访问学者
2019-01~现在, 中国科学院沈阳自动化研究所, 研究员
2016-09~2017-08,The Ohio State University, USA, 访问学者
2016-01~2018-12,中国科学院沈阳自动化研究所, 副研究员
2015-02~2015-12,中国科学院沈阳自动化研究所, 助理研究员
2014-03~2015-01,中国科学院沈阳自动化研究所, 助理研究员(博士生提前留所)

2020-06-01-今,IEEE, IEEE Senior Member

   

（1） 沈阳市高层次人才“拔尖人才”, 特等奖, 市地级, 2020
（2） 辽宁省“百千万人才工程”千层次, 特等奖, 省级, 2020
（3） 国家自然科学基金优秀青年基金, 特等奖, 国家级, 2019
（4） 辽宁省“兴辽英才计划”青年拔尖人才, 特等奖, 省级, 2019
（5） 中国科学院沈阳分院优秀青年科技人才奖, 特等奖, 院级, 2018
（6） 中国科学院沈阳自动化研究所创新2020新人新锐奖, 特等奖, 研究所（学校）, 2018
（7） 德国施普林格优秀博士学位论文(Springer Theses), 特等奖, 其他, 2018
（8） 2017 Acta Pharmacologica Sinica Outstanding Paper Award, 特等奖, 其他, 2017
（9） 中国自动化学会优秀博士学位论文, 特等奖, 国家级, 2017
（10） 中国科学院青年创新促进会会员, 特等奖, 院级, 2017
（11） 中国科学院优秀博士学位论文, 特等奖, 院级, 2016
（12） 辽宁省“百千万人才工程”万层次, 特等奖, 省级, 2016
（13） 中国科学院院长特别奖, 特等奖, 院级, 2015
（14） 中国科学院沈阳自动化研究所创新2020前沿研究奖, 特等奖, 研究所（学校）, 2015
（15） 中国科学院朱李月华优秀博士生奖, 特等奖, 院级, 2014
（16） 中国科学院大学三好学生, 特等奖, 院级, 2013
（17） 博士研究生国家奖学金, 特等奖, 国家级, 2013
（18） 中国科学院院长优秀奖, 特等奖, 院级, 2012
（19） 中国科学院研究生院三好学生, 特等奖, 院级, 2012
（20） 中国科学院大学必和必拓(BHPB)奖学金, 特等奖, 院级, 2012
（21） 博士研究生国家奖学金, 特等奖, 国家级, 2012
（22） 辽宁省自然科学学术成果奖, 二等奖, 省级, 2011
（23） 中国科学院研究生院三好学生标兵, 特等奖, 院级, 2011

​

[1] 魏佳佳, 杨艳琪, 李密. Single-cell force spectroscopy of fluid flow-tuned cell adhesion for dissecting hemodynamics in tumor metastasis. Nanoscale[J]. 2024, https://doi.org/10.1039/D3NR04439D.
[2] 杨艳琪, 冯雅琦, 魏佳佳, 李密. Multiparametric imaging and nanomechanical analysis of single native virus particles under aqueous conditions by atomic force microscopy. 生物化学与生物物理进展[J]. 2023, 50(7): 1728-1741, https://kns.cnki.net/kcms2/article/abstract?v=FbDnLXx9-bmzAYsB99Z4922hswuv2Dtb7bamrzBsqBkUkxk-Crzp1zb4z-UIhZIHU0NEk9v0EUWQBCC9-O7rdYFFtuKhU_f1ceiWdT47lGLufzxFWlkh-w==&uniplatform=NZKPT.
[3] 魏佳佳, 李密. Interplay of fluid mechanics and matrix stiffness in tuning the mechanical behaviors of single cells probed by atomic force microscopy. LANGMUIR[J]. 2023, 39(3): 1309-1319, https://pubs.acs.org/doi/10.1021/acs.langmuir.2c03137.
[4] 吕晓龙, 魏佳佳, 张志慧, 李密. Combining atomic force microscopy with optical image recognition for rapid measurements of single-cell mechanical properties. 生物化学与生物物理进展[J]. 2023, 50(8): 2018-2029, https://kns.cnki.net/kcms2/article/abstract?v=rNTUGOLzFSE2viTUG1p6n0J-jVktHvahMZ_IUE8McvDBeh4h15jvq06b1cUpwie_eqp_HSl9J0BsCecDSTdDh_oYp5E-QvSmRSE2T5N_PmVES0p_HOVkAg==&uniplatform=NZKPT.
[5] 冯雅琦, 李密. Micropipette-assisted atomic force microscopy for single-cell 3D manipulations and nanomechanical measurements. Nanoscale[J]. 2023, 15(32): 13346-13358, https://pubs.rsc.org/en/content/articlelanding/2023/nr/d3nr02404k.
[6] 吕晓龙, 李密. Deep Learning Image Recognition-assisted Atomic Force Microscopy for Precise and Efficient Detection of Single-cell Mechanical Properties. 生物化学与生物物理进展[J]. 2023, https://kns.cnki.net/kcms2/article/abstract?v=quEz3ePNgzbsKV_rLAlAdiyTMz4t3gSHtlnWSv-Hw9kfDz-QSIZZyu-8FspP98h_PIDFA0bwI-CMizLVMmdWQV1wAKF0f6P6eJjAj39tygWIXPH3tktfW6TwF6G32Gni&uniplatform=NZKPT.
[7] 李密. Combining atomic force microscopy with complementary techniques for multidimensional single-cell analysis. Journal of Microscopy[J]. 2023, 290(2): 69-96, https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.13183.
[8] 冯雅琦, 刘美辰, 李鑫鑫, 李密, 邢晓静, 刘连庆. Nanomechanical Signatures of Extracellular Vesicles from Hematologic Cancer Patients Unraveled by Atomic Force Microscopy for Liquid Biopsy. Nano Letters[J]. 2023, 23(4): 1591-1599, https://pubs.acs.org/doi/10.1021/acs.nanolett.3c00093.
[9] Li, Mi, Liu, Lianqing, Zambelli, Tomaso. FluidFM for single-cell biophysics. NANO RESEARCH[J]. 2022, 15(2): 773-786, https://link.springer.com/article/10.1007/s12274-021-3573-y.
[10] 魏佳佳, 李密, 冯雅琦, 刘连庆. Measuring the mechanical properties of cancerous cells in fluidic environments by atomic force microscopy. 生物化学与生物物理进展[J]. 2022, 49(10): 2041-2053, https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&dbname=CJFDAUTO&filename=SHSW202210018&uniplatform=NZKPT&v=22j_zxwzYyYxqvjII1F3fd5pRT2c3CvCCojQkn2r1vY2px3H_6o7fiAabOr2H4oL.
[11] 冯雅琦, 于鹏, 施佳林, 李密. Combining micropipette and atomic force microscopy for single-cell drug delivery and simultaneous cell mechanics measurement. 生物化学与生物物理进展[J]. 2022, 49(2): 420-430, https://t.cnki.net/kcms/article/abstract?v=qneGCXB6_yoPOXVBHqBqNHOeI5o1tg-Jkf1C2U6nbzwdVjZPOgkdGLmoQNSuf2590Uh7mvw-C5XsgyfM_EdO1rnMibr0kI9k-cXATvWH58xZWhhJ3GGS18mbzV21c3-G&uniplatform=NZKPT.
[12] Mi Li, Xinning Xu, Ning Xi, Wenxue Wang, Xiaojing Xing, Lianqing Liu. Multiparametric atomic force microscopy imaging of single native exosomes. ACTA BIOCHIMICA ET BIOPHYSICA SINICA[J]. 2021, 53(3): 385-388, http://dx.doi.org/10.1093/abbs/gmaa172.
[13] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Atomic force microscopy for revealing micro/nanoscale mechanics in tumor metastasis: from single cells to microenvironmental cues. ACTA PHARMACOLOGICA SINICAnull. 2021, 42(3): 323-339, http://lib.cqvip.com/Qikan/Article/Detail?id=7104498256.
[14] 李密, 席宁, 刘连庆. 基于液相AFM的植物黏液功能界面超微结构及其机械特性研究. 中国科学:技术科学[J]. 2021, 51(5): 543-553, http://lib.cqvip.com/Qikan/Article/Detail?id=7105048505.
[15] Li, Mi, Xi, Ning, Liu, Lianqing. Peak force tapping atomic force microscopy for advancing cell and molecular biology. NANOSCALE[J]. 2021, 13(18): 8358-8375, http://dx.doi.org/10.1039/d1nr01303c.
[16] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Progress in Nanorobotics for Advancing Biomedicine. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING[J]. 2021, 68(1): 130-147, https://www.webofscience.com/wos/woscc/full-record/WOS:000602691200014.
[17] Mi Li, XinNing Xu, Ning Xi, WenXue Wang, XiaoJing Xing, Lianqing Liu. Nanostructures and Mechanics of Living Exosomes Probed by Atomic Force Microscopy. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2021, 48(1): 100-110, https://www.webofscience.com/wos/woscc/full-record/WOS:000612473100009.
[18] Li, Mi, Xi, Ning, Liu, Lianqing. Hierarchical Micro-/Nanotopography for Tuning Structures and Mechanics of Cells Probed by Atomic Force Microscopy. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2021, 20(4): 543-553, http://dx.doi.org/10.1109/TNB.2021.3096056.
[19] 李密, 许新宁, 席宁, 王文学, 邢晓静, 刘连庆. 基于AFM的活体状态外泌体纳米结构及机械特性研究（英文）. 生物化学与生物物理进展[J]. 2020, 100-110, https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKMypi3qVj28LEUDxQXHYyS3fbyEOrIfBzH9VRyF39XUyG_rIm8tS3eVbzDanryNW2Nl0XKIGaQYm&uniplatform=NZKPT.
[20] 李密, 席宁, 王越超, 刘连庆. 基于AFM的食虫植物天然水凝胶黏液纳米结构原位成像与分析. 中国科学:生命科学[J]. 2020, 50(6): 650-660, http://lib.cqvip.com/Qikan/Article/Detail?id=7102303929.
[21] Liu, Bin, Dang, Dan, Yang, Yang, Yu, Peng, Li, Mi. Nanoscale imaging and mechanical analysis of viral infection on cells by atomic force microscopy. ACTA BIOCHIMICA ET BIOPHYSICA SINICA[J]. 2020, 52(11): 1289-1292, http://lib.cqvip.com/Qikan/Article/Detail?id=7104154192.
[22] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Nanoscale Organization and Functional Analysis of Carnivorous Plant Mucilage by Atomic Force Microscopy. IEEE TRANSACTIONS ON NANOTECHNOLOGY[J]. 2020, 19: 579-593, https://www.webofscience.com/wos/woscc/full-record/WOS:000554886000003.
[23] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2020, 19(1): 78-99, http://dx.doi.org/10.1109/TNB.2019.2954099.
[24] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. In Situ High-Resolution AFM Imaging and Force Probing of Cell Culture Medium-Forming Nanogranular Surfaces for Cell Growth. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2020, 19(3): 385-393, https://www.webofscience.com/wos/woscc/full-record/WOS:000545423500007.
[25] Dang Dan, Xiang RongWu, Liu Bin, Liu XiaoFei, Li Mi. Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy. PROGRESSINBIOCHEMISTRYANDBIOPHYSICS[J]. 2019, 46(1): 89-98, http://ir.sia.cn/handle/173321/24133.
[26] Li Mi, Xi Ning, Wang Yuechao, Liu Lianqing. Advances in atomic force microscopy for single-cell analysis. NANO RESEARCH[J]. 2019, 12(4): 703-718, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=6455349&detailType=1.
[27] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Nanoscale Multiparametric Imaging of Peptide-Assembled Nanofibrillar Hydrogels by Atomic Force Microscopy. IEEE TRANSACTIONS ON NANOTECHNOLOGY[J]. 2019, 18: 315-328, http://ir.sia.cn/handle/173321/24589.
[28] 党丹, 项荣武, 刘斌, 刘小菲, 李密. 基于AFM单细胞力谱技术的淋巴瘤细胞黏附力测量. 生物化学与生物物理进展[J]. 2019, 89-98, http://lib.cqvip.com/Qikan/Article/Detail?id=83728387504849574849484948.
[29] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Tunable Hybrid Biopolymeric Hydrogel Scaffolds Based on Atomic Force Microscopy Characterizations for Tissue Engineering. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2019, 18(4): 597-610, https://www.webofscience.com/wos/woscc/full-record/WOS:000498049700011.
[30] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Composite Nanostructures and Adhesion Analysis of Natural Plant Hydrogels Investigated by Atomic Force Microscopy. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2019, 18(3): 448-455, http://dx.doi.org/10.1109/TNB.2019.2911044.
[31] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Nanotopographical Surfaces for Regulating Cellular Mechanical Behaviors Investigated by Atomic Force Microscopy. ACS BIOMATERIALS SCIENCE & ENGINEERING[J]. 2019, 5(10): 5036-5050, https://www.webofscience.com/wos/woscc/full-record/WOS:000490658800015.
[32] Li, Mi, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Atomic Force Microscopy in Probing Tumor Physics for Nanomedicine. IEEE TRANSACTIONS ON NANOTECHNOLOGY[J]. 2019, 18: 83-113, http://ir.sia.cn/handle/173321/23676.
[33] 党丹, 李密, 项荣武. 基于原子力显微镜（AFM）的细胞黏弹特性测量与分析. 科学通报[J]. 2019, 64(15): 1-10, http://lib.cqvip.com/Qikan/Article/Detail?id=7002173215.
[34] Mi Li, Ning Xi, Yuechao Wang, Lianqing Liu. Advances in atomic force microscopy for single-cell analysis. 纳米研究:英文版[J]. 2019, 12(4): 703-718, http://lib.cqvip.com/Qikan/Article/Detail?id=7001880724.
[35] 李密, 席宁, 王越超, 刘连庆. 基于多参数成像AFM的细胞及分子力学特性探测研究进展. 生物化学与生物物理进展[J]. 2018, 45(11): 1106-1114, http://ir.sia.cn/handle/173321/23581.
[36] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Atomic force microscopy studies on cellular elastic and viscoelastic properties. SCIENCE CHINA-LIFE SCIENCES[J]. 2018, 61(1): 57-67, http://dx.doi.org/10.1007/s11427-016-9041-9.
[37] 李密. Investigations of cellular and molecular biophysical properties by atomic force microscopy nanorobotics. 2018, 135p-, http://ir.sia.cn/handle/173321/23754.
[38] Mi Li, Lianqing Liu, Ning Xi, Yuechao Wang. Atomic force microscopy studies on cellular elastic and viscoelastic properties. SCIENCE CHINA LIFE SCIENCES,[J]. 2018, 61(1): 57-67, https://www.webofscience.com/wos/woscc/full-record/WOS:000423730100007.
[39] Li, Mi, Dang, Dan, Xi, Ning, Wang, Yuechao, Liu, Lianqing. A Review of Nanoscale Characterizing Individual DNA Behaviors Using Atomic Force Microscopy. IEEE TRANSACTIONS ON NANOTECHNOLOGY[J]. 2018, 17(5): 920-933, https://www.webofscience.com/wos/woscc/full-record/WOS:000443975800008.
[40] Li, Mi, Liu, Lianqing, Xu, Xinning, Xing, Xiaojing, Dang, Dan, Xi, Ning, Wang, Yuechao. Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS[J]. 2018, 82: 193-201, http://dx.doi.org/10.1016/j.jmbbm.2018.03.036.
[41] Li Mi, Xi Ning, Wang YueChao, Liu LianQing. Applications of Multiparametric Imaging Atomic Force Microscopy in Probing Cellular and Molecular Mechanics. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 45(11): 1106-1114, https://www.webofscience.com/wos/woscc/full-record/WOS:000451650100002.
[42] Li, Mi, Li, Haichang, Li, Xiangguang, Zhu, Hua, Xu, Zihui, Liu, Lianqing, Ma, Jianjie, Zhang, Mingjun. A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing. ACS APPLIED MATERIALS & INTERFACES[J]. 2017, 9(27): 22160-22175, http://dx.doi.org/10.1021/acsami.7b04428.
[43] Li, Mi, Dang, Dan, Xi, Ning, Wang, Yuechao, Liu, Lianqing. Nanoscale imaging and force probing of biomolecular systems using atomic force microscopy: from single molecules to living cells. NANOSCALE[J]. 2017, 9(45): 17643-17666, https://www.webofscience.com/wos/woscc/full-record/WOS:000416825000001.
[44] Li, Mi, Liu, Lianqing, Xiao, Xiubin, Xi, Ning, Wang, Yuechao. The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy. SCIENCE CHINA-MATERIALS[J]. 2017, 60(3): 269-278, https://www.sciengine.com/doi/10.1007/s40843-016-5152-2.
[45] Li, Mi, Dang, Dan, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Imaging and Force Recognition of Single Molecular Behaviors Using Atomic Force Microscopy. SENSORS[J]. 2017, 17(1): http://www.irgrid.ac.cn/handle/1471x/1161345.
[46] 李密, 刘连庆, 席宁, 王越超. 基于AFM的细胞弹性及黏弹性研究. 中国科学:生命科学[J]. 2017, 629-639, [47] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Applications of Micro/Nano Automation Technology in Detecting Cancer Cells for Personalized Medicine. IEEE TRANSACTIONS ON NANOTECHNOLOGY[J]. 2017, 16(2): 217-229, http://dx.doi.org/10.1109/TNANO.2017.2654320.
[48] Li, Mi, Dang, Dan, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2017, 16(6): 523-540, http://dx.doi.org/10.1109/TNB.2017.2714462.
[49] 刘斌, 李密, 刘柱, 王博, 刘连庆. 原子力显微术应用于单细胞水平肿瘤研究的进展. 电子显微学报[J]. 2017, 36(6): 610-623, http://lib.cqvip.com/Qikan/Article/Detail?id=673975146.
[50] 李密, 刘连庆, 肖秀斌, 席宁, 王越超. 基于AFM的化疗药物与质粒DNA间动态相互作用观测. 中国科学：材料科学（英文版）[J]. 2017, 60(3): 269-278, http://lib.cqvip.com/Qikan/Article/Detail?id=671482068.
[51] Ma Jianjie, Li Xiangguang, Zhang Mingjun, 李密, Zhu Hua, Li Haichang, Xu Zihui, 刘连庆. A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing. ACS APPLIED MATERIALS AND INTERFACES[J]. 2017, 9(27): 22160-22175, https://www.webofscience.com/wos/woscc/full-record/WOS:000405764700011.
[52] Li, Mi, Xiao, Xiubin, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Rapid recognition and functional analysis of membrane proteins on human cancer cells using atomic force microscopy. JOURNAL OF IMMUNOLOGICAL METHODS[J]. 2016, 436: 41-49, http://dx.doi.org/10.1016/j.jim.2016.06.006.
[53] Li Mi, Liu Lianqing, Xi Ning, Wang Yuechao. Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale. BIONANOSCIENCE[J]. 2016, 6(1): 22-32, http://www.irgrid.ac.cn/handle/1471x/1035159.
[54] Li, Mi, Liu, Lianqing, Xiao, Xiubin, Xi, Ning, Wang, Yuechao. Effects of methotrexate on the viscoelastic properties of single cells probed by atomic force microscopy. JOURNAL OF BIOLOGICAL PHYSICS[J]. 2016, 42(4): 551-569, https://www.webofscience.com/wos/woscc/full-record/WOS:000385191200004.
[55] Li, Mi, Xiao, Xiubin, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Nanoscale Quantifying the Effects of Targeted Drug on Chemotherapy in Lymphoma Treatment Using Atomic Force Microscopy. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING[J]. 2016, 63(10): 2187-2199, http://www.irgrid.ac.cn/handle/1471x/1142426.
[56] Li, Mi, Liu, Lianqing, Xiao, Xiubin, Xi, Ning, Wang, Yuechao. Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2016, 15(5): 398-411, http://www.irgrid.ac.cn/handle/1471x/1142427.
[57] 李密, 刘连庆, 席宁, 王越超. 基于AFM的临床原代细胞机械特性测量研究进展. 中国科学：生命科学[J]. 2016, 46(12): 1370-1381, http://lib.cqvip.com/Qikan/Article/Detail?id=670876743.
[58] 李密, 刘连庆, 席宁, 王越超. 基于AFM的细胞表面超微形貌成像与机械特性测量研究进展. 生物化学与生物物理进展[J]. 2015, 42(8): 697-712, http://www.irgrid.ac.cn/handle/1471x/978845.
[59] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Xiao XiuBin, Zhang WeiJing. Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy. SCIENCE CHINA-LIFE SCIENCES[J]. 2015, 58(9): 889-901, http://www.irgrid.ac.cn/handle/1471x/1008388.
[60] Li Mi, Liu Lianqing, Xi Ning, Wang Yuechao. Biological Applications of a Nanomanipulator Based on AFM In situ visualization and quantification of cellular behaviors at the single-molecule level. IEEE NANOTECHNOLOGY MAGAZINE. 2015, 9(3): 25-35, http://www.irgrid.ac.cn/handle/1471x/975972.
[61] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy. ACTA PHARMACOLOGICA SINICA[J]. 2015, 36(7): 769-782, http://lib.cqvip.com/Qikan/Article/Detail?id=665568148.
[62] Li Mi, Liu LianQing, Xi Ning, Wang YueChao. In situ Imaging The Cellular Ultra-microstructures and Measuring The Cellular Mechanical Properties Using Atomic Force Microscopy. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2015, 42(8): 697-712, https://www.webofscience.com/wos/woscc/full-record/WOS:000359967100002.
[63] Mi LI, Lian-qing LIU, Ning Xi, Yue-chao WANG. Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy. 中国药理学报：英文版[J]. 2015, 769-782, http://lib.cqvip.com/Qikan/Article/Detail?id=665568148.
[64] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Xiao, Xiubin, Zhang, Weijing. Quantitative Analysis of Drug-Induced Complement-Mediated Cytotoxic Effect on Single Tumor Cells Using Atomic Force Microscopy and Fluorescence Microscopy. IEEE TRANSACTIONS ON NANOBIOSCIENCE[J]. 2015, 14(1): 84-94, http://www.irgrid.ac.cn/handle/1471x/934156.
[65] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Xiao, Xiubin, Zhang, Weijing. Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells. LANGMUIR[J]. 2014, 30(6): 1609-1621, http://www.irgrid.ac.cn/handle/1471x/834582.
[66] 李密, 刘连庆, 席宁, 王越超. AFM单分子力谱技术测量膜蛋白力学特性的研究进展. 科学通报[J]. 2014, 59(13): 1198-1208, http://www.irgrid.ac.cn/handle/1471x/852515.
[67] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy. CHINESE SCIENCE BULLETIN[J]. 2014, 59(31): 4020-4029, http://www.irgrid.ac.cn/handle/1471x/900835.
[68] Li, Mi, Xiao, Xiubin, Zhang, Weijing, Liu, Lianqing, Xi, Ning, Wang, Yuechao. AFM analysis of the multiple types of molecular interactions involved in rituximab lymphoma therapy on patient tumor cells and NK cells. CELLULAR IMMUNOLOGY[J]. 2014, 290(2): 233-244, http://dx.doi.org/10.1016/j.cellimm.2014.07.003.
[69] Li, M, Xiao, X, Zhang, W, Liu, L, Xi, N, Wang, Y. Nanoscale distribution of CD20 on B-cell lymphoma tumour cells and its potential role in the clinical efficacy of rituximab. JOURNAL OF MICROSCOPY[J]. 2014, 254(1): 19-30, http://www.irgrid.ac.cn/handle/1471x/842239.
[70] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao. Progress in measuring biophysical properties of membrane proteins with AFM single-molecule force spectroscopy. CHINESE SCIENCE BULLETIN[J]. 2014, 59(22): 2717-2725, http://www.irgrid.ac.cn/handle/1471x/842241.
[71] Li, Mi, Xiao, Xiubin, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Dong, Zaili, Zhang, Weijing. Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients. EXPERIMENTAL CELL RESEARCH[J]. 2013, 319(18): 2812-2821, http://dx.doi.org/10.1016/j.yexcr.2013.07.020.
[72] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Xiao XiuBin, Zhang WeiJing. Progress of AFM single-cell and single-molecule morphology imaging. CHINESE SCIENCE BULLETIN[J]. 2013, 58(26): 3177-3182, http://www.irgrid.ac.cn/handle/1471x/720193.
[73] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 基于AFM的哺乳动物活细胞成像. 中国科学:生命科学[J]. 2013, 43(9): 770-777, http://www.irgrid.ac.cn/handle/1471x/756142.
[74] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Xiao, Xiubin, Zhang, Weijing. Imaging and measuring the biophysical properties of Fc gamma receptors on single macrophages using atomic force microscopy. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2013, 438(4): 709-714, http://www.irgrid.ac.cn/handle/1471x/756174.
[75] Li, Mi, Xiao, Xiubin, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Dong, Zaili, Zhang, Weijing. Imaging and Measuring the Molecular Force of Lymphoma Pathological Cells Using Atomic Force Microscopy. SCANNING[J]. 2013, 35(1): 40-46, http://www.irgrid.ac.cn/handle/1471x/622922.
[76] Li, Mi, Xiao, Xiubin, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Dong, Zaili, Zhang, Weijing. Atomic force microscopy study of the antigen-antibody binding force on patient cancer cells based on ROR1 fluorescence recognition. JOURNAL OF MOLECULAR RECOGNITION[J]. 2013, 26(9): 432-438, http://dx.doi.org/10.1002/jmr.2287.
[77] Li, Mi, Zhang, Changlin, Wang, Liu, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Dong, Zaili. Investigating the morphology and mechanical properties of blastomeres with atomic force microscopy. SURFACE AND INTERFACE ANALYSIS[J]. 2013, 45(8): 1193-1196, http://www.irgrid.ac.cn/handle/1471x/622924.
[78] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Xiao XiuBin, Zhang WeiJing. Atomic force microscopy imaging of live mammalian cells. SCIENCE CHINA-LIFE SCIENCES[J]. 2013, 56(9): 811-817, http://www.irgrid.ac.cn/handle/1471x/720180.
[79] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Xiao XiuBin, Zhang WeiJing. Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy. CHINESE SCIENCE BULLETIN[J]. 2013, 58(13): 1516-1519, http://www.irgrid.ac.cn/handle/1471x/720185.
[80] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. AFM单细胞单分子形貌成像的研究进展. 科学通报[J]. 2013, 58(18): 1711-1718, http://www.irgrid.ac.cn/handle/1471x/720179.
[81] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. Investigation of protein-protein interactions in cancer targeted therapy using nanorobots. SELECTED TOPICS IN MICRO/NANO-ROBOTICS FOR BIOMEDICAL APPLICATIONS. 2012, 125-158, http://www.irgrid.ac.cn/handle/1471x/531663.
[82] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Xiao XiuBin, Zhang WeiJing. Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells. SCIENCE CHINA-LIFE SCIENCES[J]. 2012, 55(11): 968-973, http://www.irgrid.ac.cn/handle/1471x/531606.
[83] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Xiao XiuBin, Zhang WeiJing. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy. ACTA PHYSICO-CHIMICA SINICA[J]. 2012, 28(6): 1502-1508, http://www.irgrid.ac.cn/handle/1471x/509365.
[84] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 基于AFM的红细胞及不同侵袭程度癌细胞的成像及机械特性测量. 中国科学. 生命科学[J]. 2012, 42(11): 919-925, https://www.sciengine.com/doi/10.1360/zc2012-42-11-919.
[85] Li Mi, Liu Lianqing, Xi Ning, Wang Yuechao, Dong Zaili, Xiao Xiubin, Zhang Weijing. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy. 物理化学学报[J]. 2012, 28(6): 1502-1508, http://www.irgrid.ac.cn/handle/1471x/509365.
[86] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化（英文）. 物理化学学报[J]. 2012, 28(6): 1502-1508, http://lib.cqvip.com/Qikan/Article/Detail?id=42067046.
[87] LI Mi, LIU LianQing, XI Ning, WANG YueChao, DONG ZaiLi, LI GuangYong, XIAO XiuBin, ZHANG WeiJing. Detecting CD20-Rituximab interaction forces using AFM single-molecule force spectroscopy. 中国科学通报：英文版[J]. 2011, 56(35): 3829-3835, http://lib.cqvip.com/Qikan/Article/Detail?id=40118775.
[88] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Li GuangYong, Xiao XiuBin, Zhang WeiJing. Detecting CD20-Rituximab interaction forces using AFM single-molecule force spectroscopy. CHINESE SCIENCE BULLETIN[J]. 2011, 56(35): 3829-3835, http://lib.cqvip.com/Qikan/Article/Detail?id=40118775.
[89] Li, Mi, Liu, Lianqing, Xi, Ning, Wang, Yuechao, Dong, Zaili, Tabata, Osamu, Xiao, Xiubin, Zhang, Weijing. Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2011, 404(2): 689-694, http://www.irgrid.ac.cn/handle/1471x/443018.
[90] 李密, 刘连庆, 席宁, 王越超, 董再励, 李广勇, 肖秀斌, 张伟京. 基于AFM单分子力谱技术的CD20．Rituximab间相互作用力测量. 科学通报[J]. 2011, 56(32): 2681-2688, http://lib.cqvip.com/Qikan/Article/Detail?id=39914417.
[91] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 基于AFM的淋巴瘤细胞成像及其机械特性测定. 科学通报[J]. 2010, 2188-2196, http://lib.cqvip.com/Qikan/Article/Detail?id=34807589.
[92] Li Mi, Liu LianQing, Xi Ning, Wang YueChao, Dong ZaiLi, Li GuangYong, Xiao XiuBin, Zhang WeiJing. Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy. SCIENCE CHINA-LIFE SCIENCES[J]. 2010, 53(10): 1189-1195, http://www.irgrid.ac.cn/handle/1471x/442967.
[93] 李密, 刘连庆, 席宁, 王越超, 董再励, 李广勇, 肖秀斌, 张伟京. 利用AFM探测淋巴瘤细胞表面CD20抗原与其抗体的相互作用. 中国科学：生命科学[J]. 2010, 1047-1054, http://lib.cqvip.com/Qikan/Article/Detail?id=36070994.
[94] 李密, 刘连庆, 席宁, 王越超, 董再励, 肖秀斌, 张伟京. 基于AFM的淋巴瘤细胞成像及其机械特性测定. 科学通报[J]. 2010, 2188-2196, http://lib.cqvip.com/Qikan/Article/Detail?id=34807589.
[95] Li, Mi, Liu, LianQing, Xi, Ning, Wang, YueChao, Dong, ZaiLi, Li, GuangYong, Xiao, XiuBin, Zhang, WeiJing. Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy. SCIENCE CHINA LIFE SCIENCES,[J]. 2010, 53(10): 1189-1195, http://www.irgrid.ac.cn/handle/1471x/442967.

（1） Investigation of protein-protein interactions in cancer targeted therapy using nanorobots, Springer, 2013-09, 第 1 作者
（2） Investigating the molecular specific interactions on cell surface using atomic force microscopy, Wiley, 2015-10, 第 1 作者
（3） Investigations of cellular and molecular biophysical properties by atomic force microscopy nanorobotics, Springer, 2018-01, 第 1 作者
（4） Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells, Elsevier, 2023-02, 第 1 作者
（5） Nanorobotics for investigating cell mechanics based on atomic force microscopy, Elsevier, 2023-04, 第 1 作者

   

（ 1 ） 国家自然科学基金青年基金 “基于纳米操作机器人的癌细胞机械特征建模与检测方法研究”, 负责人, 国家任务, 2016-01--2018-12
（ 2 ） 中国科学院青年创新促进会人才专项经费, 负责人, 中国科学院计划, 2017-01--2020-12
（ 3 ） 机器人学国家重点实验室自主青年课题, 负责人, 研究所自选, 2015-01--2016-12
（ 4 ） 国家自然科学基金面上项目 "基于微纳机器人技术的癌症转移过程中细胞动态力学特性研究", 负责人, 国家任务, 2019-01--2022-12
（ 5 ） 国家自然科学基金优秀青年基金 "微纳机器人系统及其生物医学应用", 负责人, 国家任务, 2020-01--2022-12
（ 6 ） 中国科学院前沿科学重点研究计划 "微纳机器人多尺度生理特征探测及癌症应用", 负责人, 中国科学院计划, 2019-09--2024-08
（ 7 ） 辽宁省“兴辽英才计划”青年拔尖人才专项经费, 负责人, 地方任务, 2020-01--2022-12
（ 8 ） 国家自然科学基金面上项目 "面向肿瘤个性化诊疗的微纳机器人单细胞精准激励与流变特性同步检测", 负责人, 国家任务, 2023-01--2026-12

已指导学生

魏佳佳  硕士研究生  081101-控制理论与控制工程  

现指导学生

杨艳琪  硕士研究生  081102-检测技术与自动化装置  

冯雅琦  博士研究生  081104-模式识别与智能系统  

孟子洋  硕士研究生  081104-模式识别与智能系统